Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003546

Physical Location & Seq

Genomic Coordinates Reverse (-)

35510691 .. 35511287

Gene Structure 597 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1244200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 597 bp

ATGAACACGTTTGAAGCCCTTAAGCTTGTCATCCCACCTCCTGTTTTTTCTCTTCTTTCTTTCATAAGCACTATGATTTTCGTATATTTCTGCATCATAGCATTCTCCATCATACTTACGTTGATTGTCATCGCCCTCTTTTATGACATCTGCGAGTTCGTCAGGACGCCCTATTACGAGAGGAGTAGTAACCCTGATACTGAAGCCGGGGATCAACAACGATTGTTTCGACTAGACAACGGTTCAGAATGGAACGACCAAGCTACATTTTATCAAGCAACCCTCCTTCATAATTTAAGGGTTTTGGAAATTTTGGACTCGATTGTTGAGGCATTGGAGGAAAGAAGAGACGAGAGGCAGCAACGAGAAAGGGAACAAGTTTTGGAGAGCAAAATGCCACCAGCTATAGCTTATGGAAGCCAAGAAATTAAAATTAACTGTAAAGATTGTGTTATTTGCATGGAGGGTTTTGAGACCGATCAATTGTGTCAAGTTTTTCCCTCATGCAATCATACGTTCCATTCCAACTGCATGAACACTTGGTTGAAGAAGAGCCTAACTTGCCCGATTTGTCGTAATTGTGTGTTGGATTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

199

Amino Acids

22.99

Weight (kDa)

4.83

Isoelectric Point (pI)

47.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	147 - 192	2e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	149 - 192	7.4e-13	Ring finger domain
zf-RING_11	PF17123	149 - 177	2.1e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	150 - 191	1.2e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17450 AT4G35480
fragaria_vesca	FvH4_3g20200 FvH4_5g05151
malus_domestica	MD03G1226600.v1.1 MD03G1226700.v1.1 MD06G1176900.v1.1 MD11G1244200.v1.1 MD11G1244300.v1.1 MD11G1244400.v1.1 MD14G1183100.v1.1
prunus_persica	Prupe.4G182700_v2.0.a1 Prupe.4G182800_v2.0.a1 Prupe.5G175600_v2.0.a1 Prupe.6G077100_v2.0.a1
pyrus_communis	pycom06g15700
rosa_chinensis	RchiOBHm_Chr5g0034021 RchiOBHm_Chr7g0193811
rosa_laevigata	RLG00000004243
rosa_multiflora	Rmu_sc0001436.1_g000029
rosa_roxburghii	Rroxscaffold_1G00046210 Rroxscaffold_3G00261810
rosa_rugosa	Rorug07G0012200
rosa_samantha	Rh5AG235200 Rh5BG235900 Rh5CG265700 Rh5CG265800 Rh5DG242400 Rh7AG138800 Rh7BG139100 Rh7CG142800 Rh7DG140400
rosa_wichuraiana	Rw5G021230 Rw5G021450 Rw7G011830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	219
AcsI	RAATTY	1 cut(s)	309
AcuI	CTGAAG	1 cut(s)	222
AcyI	GRCGYC	1 cut(s)	167
AflII	CTTAAG	1 cut(s)	20
AflIII	ACRYGT	1 cut(s)	6
AgsI	TTSAA	2 cut(s)	14, 547
AluBI	AGCT	4 cut(s)	25, 263, 404, 410
AluI	AGCT	4 cut(s)	25, 263, 404, 410
Alw26I	GTCTC	2 cut(s)	342, 467
AlwI	GGATC	1 cut(s)	219
ApeKI	GCWGC	1 cut(s)	358
ApoI	RAATTY	1 cut(s)	309
AsuC2I	CCSGG	1 cut(s)	208
BbvI	GCAGC	1 cut(s)	370
BccI	CCATC	1 cut(s)	116
BcnI	CCSGG	1 cut(s)	208
BcoDI	GTCTC	2 cut(s)	342, 467
BfaI	CTAG	1 cut(s)	233
BfmI	CTRYAG	1 cut(s)	405
BfrI	CTTAAG	1 cut(s)	20
BisI	GCNGC	1 cut(s)	359
BlsI	GCNGC	1 cut(s)	360
Bme1390I	CCNGG	1 cut(s)	208
BmrFI	CCNGG	1 cut(s)	208
BmsI	GCATC	1 cut(s)	102
BpuMI	CCSGG	1 cut(s)	208
BsaBI	GATNNNNATC	1 cut(s)	128
BsaHI	GRCGYC	1 cut(s)	167
BsaI	GGTCTC	1 cut(s)	467
BsaJI	CCNNGG	1 cut(s)	207
Bse8I	GATNNNNATC	1 cut(s)	128
BseDI	CCNNGG	1 cut(s)	207
BseGI	GGATG	1 cut(s)	30
BseJI	GATNNNNATC	1 cut(s)	128
BseRI	GAGGAG	1 cut(s)	196
BseXI	GCAGC	1 cut(s)	370
BsiSI	CCGG	1 cut(s)	207
BsmAI	GTCTC	2 cut(s)	342, 467
BsmBI	CGTCTC	1 cut(s)	342
BsmI	GAATGC	1 cut(s)	101
Bso31I	GGTCTC	1 cut(s)	467
Bsp143I	GATC	2 cut(s)	211, 478
BspPI	GGATC	1 cut(s)	219
BspQI	GCTCTTC	1 cut(s)	545
BspTI	CTTAAG	1 cut(s)	20
BspTNI	GGTCTC	1 cut(s)	467
BssECI	CCNNGG	1 cut(s)	207
BssMI	GATC	2 cut(s)	211, 478
BssNI	GRCGYC	1 cut(s)	167
Bst4CI	ACNGT	2 cut(s)	242, 440
Bst6I	CTCTTC	3 cut(s)	57, 340, 545
BstACI	GRCGYC	1 cut(s)	167
BstAFI	CTTAAG	1 cut(s)	20
BstF5I	GGATG	1 cut(s)	30
BstKTI	GATC	2 cut(s)	214, 481
BstMAI	GTCTC	2 cut(s)	342, 467
BstMBI	GATC	2 cut(s)	211, 478
BstMWI	GCNNNNNNNGC	1 cut(s)	561
BstSCI	CCNGG	1 cut(s)	206
BstSFI	CTRYAG	1 cut(s)	405
BstV1I	GCAGC	1 cut(s)	370
BtgZI	GCGATG	1 cut(s)	115
BtsCI	GGATG	1 cut(s)	30
CseI	GACGC	1 cut(s)	175
CviAII	CATG	3 cut(s)	460, 504, 532
CviJI	RGCY	8 cut(s)	17, 25, 206, 263, 404, 410, 420, 555
CviKI_1	RGCY	8 cut(s)	17, 25, 206, 263, 404, 410, 420, 555
DpnI	GATC	2 cut(s)	213, 480
DpnII	GATC	2 cut(s)	211, 478
Eam1104I	CTCTTC	3 cut(s)	57, 340, 545
EarI	CTCTTC	3 cut(s)	57, 340, 545
Eco31I	GGTCTC	1 cut(s)	467
Eco57I	CTGAAG	1 cut(s)	222
Esp3I	CGTCTC	1 cut(s)	342
FaeI	CATG	3 cut(s)	463, 507, 535
FatI	CATG	3 cut(s)	459, 503, 531
Fnu4HI	GCNGC	1 cut(s)	359
FokI	GGATG	1 cut(s)	17
Fsp4HI	GCNGC	1 cut(s)	359
FspBI	CTAG	1 cut(s)	233
GluI	GCNGC	1 cut(s)	359
HapII	CCGG	1 cut(s)	207
HgaI	GACGC	1 cut(s)	175
Hin1I	GRCGYC	1 cut(s)	167
Hin1II	CATG	3 cut(s)	463, 507, 535
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	1 cut(s)	317
HpaII	CCGG	1 cut(s)	207
Hpy188I	TCNGA	1 cut(s)	247
Hpy188III	TCNNGA	1 cut(s)	163
HpyAV	CCTTC	1 cut(s)	296
HpyCH4III	ACNGT	2 cut(s)	242, 440
HpyCH4IV	ACGT	3 cut(s)	8, 119, 515
HpyCH4V	TGCA	4 cut(s)	93, 459, 507, 531
HpyF10VI	GCNNNNNNNGC	1 cut(s)	561
HpySE526I	ACGT	3 cut(s)	8, 119, 515
Hsp92I	GRCGYC	1 cut(s)	167
Hsp92II	CATG	3 cut(s)	463, 507, 535
Kzo9I	GATC	2 cut(s)	211, 478
LguI	GCTCTTC	1 cut(s)	545
LpnPI	CCDG	5 cut(s)	54, 148, 207, 220, 414
Lsp1109I	GCAGC	1 cut(s)	370
LweI	GCATC	1 cut(s)	102
MaeI	CTAG	1 cut(s)	233
MaeII	ACGT	3 cut(s)	8, 119, 515
MaeIII	GTNAC	1 cut(s)	188
MalI	GATC	2 cut(s)	213, 480
MboI	GATC	2 cut(s)	211, 478
MboII	GAAGA	4 cut(s)	44, 357, 559, 562
MfeI	CAATTG	1 cut(s)	482
MluCI	AATT	6 cut(s)	292, 309, 426, 432, 482, 577
MlyI	GAGTC	1 cut(s)	311
MmeI	TCCRAC	2 cut(s)	549, 567
MnlI	CCTC	9 cut(s)	48, 146, 174, 293, 322, 331, 348, 457, 511
MseI	TTAA	4 cut(s)	21, 296, 429, 435
MspCI	CTTAAG	1 cut(s)	20
MspI	CCGG	1 cut(s)	207
MspR9I	CCNGG	1 cut(s)	208
MunI	CAATTG	1 cut(s)	482
Mva1269I	GAATGC	1 cut(s)	101
MwoI	GCNNNNNNNGC	1 cut(s)	561
NciI	CCSGG	1 cut(s)	208
NdeII	GATC	2 cut(s)	211, 478
NlaIII	CATG	3 cut(s)	463, 507, 535
PciSI	GCTCTTC	1 cut(s)	545
PcsI	WCGNNNNNNNCGW	1 cut(s)	226
PctI	GAATGC	1 cut(s)	101
PkrI	GCNGC	1 cut(s)	360
PleI	GAGTC	1 cut(s)	311
PpsI	GAGTC	1 cut(s)	311
SapI	GCTCTTC	1 cut(s)	545
SaqAI	TTAA	4 cut(s)	21, 296, 429, 435
SatI	GCNGC	1 cut(s)	359
Sau3AI	GATC	2 cut(s)	211, 478
SchI	GAGTC	1 cut(s)	311
ScrFI	CCNGG	1 cut(s)	208
SetI	ASST	8 cut(s)	11, 27, 40, 122, 265, 406, 412, 518
SfaNI	GCATC	1 cut(s)	102
SfcI	CTRYAG	1 cut(s)	405
SmlI	CTYRAG	1 cut(s)	20
SmoI	CTYRAG	1 cut(s)	20
Sse9I	AATT	6 cut(s)	292, 309, 426, 432, 482, 577
SspMI	CTAG	1 cut(s)	233
StyD4I	CCNGG	1 cut(s)	206
TaaI	ACNGT	2 cut(s)	242, 440
TaiI	ACGT	3 cut(s)	11, 122, 518
TaqI	TCGA	2 cut(s)	229, 320
TaqII	GACCGA	1 cut(s)	491
TasI	AATT	6 cut(s)	292, 309, 426, 432, 482, 577
Tru1I	TTAA	4 cut(s)	21, 296, 429, 435
Tru9I	TTAA	4 cut(s)	21, 296, 429, 435
TseI	GCWGC	1 cut(s)	358
TspDTI	ATGAA	4 cut(s)	17, 52, 278, 548
Vha464I	CTTAAG	1 cut(s)	20
XapI	RAATTY	1 cut(s)	309
XspI	CTAG	1 cut(s)	233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.