MD03G1226600.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003546
Physical Location & Seq
Forward (+)
31191302 .. 31191844
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1226600.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTTTTATGAAGCCTTCGTTTCGGAACCCGGTCCTCCTCTATTTTCAGAGCTTCCAGCTAAGATCATTGCATGCATCTCAATAGTCATTTTGTTATTCTTGATTTTCAATCACATTCTCAATGTGTCCCTTGCCTGGTTCCTAGAAATGGTCCCAGAACATGATCTTGAGGGAGGATACGTCATGTACGGGCCTCGAATAAACACCATAGGTTCAAGTCCCTTTCGGATAGCTATAGTACAAAATATGCAGCGGCTGGGAGTGATAAAGAGACTCATGCCGGATTTCGATGAAGGAAGAGGGCCGAGGCTTAGGGCTTTGAAGAAACTGCCACCATTAGTGAATTATGGAAGCTGTGAAATAAGATCATCAAGTTCATCAAGGTGTACTGCTTGTGCAATCTGTTTGGAGGATTTTGCAGATGGTGATTCATGTCAAATTTTTTCACTTTGTAACCACATTTTCCATTCGAATTGCATTGATAATTGGTTGATGAACAAGCCAACTTGCCCAGTTTGTCGAAACTGTATCATCGATACATGA

Protein Analysis

181

Amino Acids

20.21

Weight (kDa)

6.06

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 131 - 174 2e-14 Ring finger domain
zf-RING_11 PF17123 132 - 160 5.5e-08 RING-like zinc finger
zf-C3HC4 PF00097 132 - 173 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 173 3.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 133 - 174 2.1e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 253
AcsI RAATTY 1 cut(s) 438
AfaI GTAC 3 cut(s) 188, 240, 388
AfiI CCNNNNNNNGG 2 cut(s) 135, 148
AgsI TTSAA 3 cut(s) 109, 216, 322
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 4 cut(s) 52, 59, 233, 354
AluI AGCT 4 cut(s) 52, 59, 233, 354
Alw26I GTCTC 1 cut(s) 265
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 2 cut(s) 191, 302
ApeKI GCWGC 1 cut(s) 250
ApoI RAATTY 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 32, 151, 191, 302
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 437
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 2 cut(s) 32, 151
BbvI GCAGC 1 cut(s) 262
BccI CCATC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 2 cut(s) 251, 254
BlsI GCNGC 2 cut(s) 252, 255
Bme1390I CCNGG 2 cut(s) 30, 136
Bme18I GGWCC 2 cut(s) 32, 151
BmgT120I GGNCC 4 cut(s) 32, 151, 191, 302
BmiI GGNNCC 3 cut(s) 27, 140, 153
BmrFI CCNGG 2 cut(s) 30, 136
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 84
BmuI ACTGGG 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 30
Bsa29I ATCGAT 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 305
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 148
Bse1I ACTGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 136
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 135, 148
BseMI GCAATG 1 cut(s) 66
BseNI ACTGG 1 cut(s) 512
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 256
BshFI GGCC 2 cut(s) 193, 304
BshVI ATCGAT 1 cut(s) 534
BsiSI CCGG 2 cut(s) 30, 281
BslFI GGGAC 3 cut(s) 112, 137, 204
BslI CCNNNNNNNGG 2 cut(s) 135, 148
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 3 cut(s) 112, 137, 204
BsnI GGCC 2 cut(s) 193, 304
Bsp119I TTCGAA 1 cut(s) 470
Bsp143I GATC 3 cut(s) 63, 163, 365
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 193, 304
BspDI ATCGAT 1 cut(s) 534
BspLI GGNNCC 3 cut(s) 27, 140, 153
BspT104I TTCGAA 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 66
BsrI ACTGG 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 3 cut(s) 63, 163, 365
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 527
Bst6I CTCTTC 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 60, 311
BstKTI GATC 3 cut(s) 66, 166, 368
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 3 cut(s) 63, 163, 365
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 2 cut(s) 28, 134
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 534
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 193, 304
BsuTUI ATCGAT 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 73
CaiI CAGNNNCTG 1 cut(s) 256
Cfr13I GGNCC 4 cut(s) 32, 151, 191, 302
ClaI ATCGAT 1 cut(s) 534
Csp6I GTAC 3 cut(s) 187, 239, 387
CviAII CATG 6 cut(s) 72, 161, 184, 277, 432, 540
CviQI GTAC 3 cut(s) 187, 239, 387
DdeI CTNAG 2 cut(s) 60, 311
DpnI GATC 3 cut(s) 65, 165, 367
DpnII GATC 3 cut(s) 63, 163, 365
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 32, 151
EcoRII CCWGG 1 cut(s) 134
EcoT22I ATGCAT 1 cut(s) 77
FaeI CATG 6 cut(s) 75, 164, 187, 280, 435, 543
FaqI GGGAC 3 cut(s) 112, 137, 204
FatI CATG 6 cut(s) 71, 160, 183, 276, 431, 539
Fnu4HI GCNGC 2 cut(s) 251, 254
Fsp4HI GCNGC 2 cut(s) 251, 254
FspBI CTAG 1 cut(s) 143
GluI GCNGC 2 cut(s) 251, 254
GsaI CCCAGC 1 cut(s) 260
HaeIII GGCC 2 cut(s) 193, 304
HapII CCGG 2 cut(s) 30, 281
Hin1II CATG 6 cut(s) 75, 164, 187, 280, 435, 543
HinfI GANTC 2 cut(s) 273, 428
HpaII CCGG 2 cut(s) 30, 281
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 3 cut(s) 25, 49, 228
Hpy188III TCNNGA 2 cut(s) 100, 167
Hpy8I GTNNAC 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 25, 287
HpyCH4III ACNGT 1 cut(s) 527
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 6 cut(s) 71, 75, 250, 398, 419, 477
HpyF3I CTNAG 2 cut(s) 60, 311
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 6 cut(s) 75, 164, 187, 280, 435, 543
Kzo9I GATC 3 cut(s) 63, 163, 365
LpnPI CCDG 8 cut(s) 43, 69, 121, 148, 168, 242, 294, 525
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 143
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 452
MalI GATC 3 cut(s) 65, 165, 367
MboI GATC 3 cut(s) 63, 163, 365
MboII GAAGA 2 cut(s) 309, 334
MluCI AATT 4 cut(s) 343, 438, 472, 484
MlyI GAGTC 1 cut(s) 267
MnlI CCTC 8 cut(s) 45, 48, 163, 167, 204, 293, 300, 403
Mph1103I ATGCAT 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 2 cut(s) 30, 281
MspR9I CCNGG 2 cut(s) 30, 136
MvaI CCWGG 1 cut(s) 136
NciI CCSGG 1 cut(s) 30
NdeII GATC 3 cut(s) 63, 163, 365
NlaIII CATG 6 cut(s) 75, 164, 187, 280, 435, 543
NlaIV GGNNCC 3 cut(s) 27, 140, 153
NmeAIII GCCGAG 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 77
NspI RCATGY 1 cut(s) 75
NspV TTCGAA 1 cut(s) 470
PaeI GCATGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 2 cut(s) 252, 255
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 3 cut(s) 27, 140, 153
PspPI GGNCC 4 cut(s) 32, 151, 191, 302
PstNI CAGNNNCTG 1 cut(s) 256
RsaI GTAC 3 cut(s) 188, 240, 388
RsaNI GTAC 3 cut(s) 187, 239, 387
RseI CAYNNNNRTG 1 cut(s) 382
SatI GCNGC 2 cut(s) 251, 254
Sau3AI GATC 3 cut(s) 63, 163, 365
Sau96I GGNCC 4 cut(s) 32, 151, 191, 302
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 30, 136
SetI ASST 7 cut(s) 54, 61, 183, 214, 235, 356, 386
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 2 cut(s) 32, 151
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SphI GCATGC 1 cut(s) 75
Sse9I AATT 4 cut(s) 343, 438, 472, 484
SsiI CCGC 1 cut(s) 253
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 28, 134
TaaI ACNGT 1 cut(s) 527
TaiI ACGT 1 cut(s) 183
TaqI TCGA 5 cut(s) 196, 288, 470, 520, 534
TasI AATT 4 cut(s) 343, 438, 472, 484
TatI WGTACW 2 cut(s) 238, 386
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 1 cut(s) 428
TseI GCWGC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 24, 306, 366, 420, 509
VpaK11BI GGWCC 2 cut(s) 32, 151
XapI RAATTY 1 cut(s) 438
XceI RCATGY 1 cut(s) 75
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.