Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003546

Physical Location & Seq

Genomic Coordinates Forward (+)

31194495 .. 31195085

Gene Structure 591 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1226700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 591 bp

ATGTTTGAAGCCCTGAAGGTCGTCACCCCACCTCCTGTTTTTTCTCTTCTTTCTTTGATCAGCAAGATAATTTTCGTATATGTCTGCATCATAGCATTCTCCATCATGCTTTTGTTGATCGTCATTGCCCTCCTTTACGTCATCTGCGAGTTTGTTTGGACGCCCTTTTACGAGAGGAGAAGTAATCCTGATCCCGAAGCCGAGGATCAACAACAATTATCTCGCCCAAACAATGGTGCAGAATGGAATGACCAAGCTACATTTTATCAAGCAGCCGTCCTTCATAATTTGAGGGTTTTGGAAATTTTGGACTCGATTGTTGAGGTTTTGGAGGAAAGAAGAGACGAAAGGCAGCAGCGAGTAAGGGAACAAGTTTTGGAGAGCAAAATGCCACCACCTATAGCTTATGGAAGCCAAGAAACTGAAATTAATTGCAAGGATTGTGTTATTTGCATGGAGGATTTTGAGAACGACCAATTGTGTCAAGTTTTTCCCTCATGCAATCATACGTTCCATTCCAATTGTATGAACACTTGGTTGAAGAAGAGCCTAACTTGCCCGATTTGTCGTAATTCTGTATTGGACTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

197

Amino Acids

22.71

Weight (kDa)

4.75

Isoelectric Point (pI)

52.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	146 - 190	1.8e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	147 - 190	3e-13	Ring finger domain
zf-RING_11	PF17123	147 - 175	3.2e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	148 - 189	6.5e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	148 - 187	1.4e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0003546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17450 AT4G35480
fragaria_vesca	FvH4_3g20200 FvH4_5g05151
malus_domestica	MD03G1226600.v1.1 MD03G1226700.v1.1 MD06G1176900.v1.1 MD11G1244200.v1.1 MD11G1244300.v1.1 MD11G1244400.v1.1 MD14G1183100.v1.1
prunus_persica	Prupe.4G182700_v2.0.a1 Prupe.4G182800_v2.0.a1 Prupe.5G175600_v2.0.a1 Prupe.6G077100_v2.0.a1
pyrus_communis	pycom06g15700
rosa_chinensis	RchiOBHm_Chr5g0034021 RchiOBHm_Chr7g0193811
rosa_laevigata	RLG00000004243
rosa_multiflora	Rmu_sc0001436.1_g000029
rosa_roxburghii	Rroxscaffold_1G00046210 Rroxscaffold_3G00261810
rosa_rugosa	Rorug07G0012200
rosa_samantha	Rh5AG235200 Rh5BG235900 Rh5CG265700 Rh5CG265800 Rh5DG242400 Rh7AG138800 Rh7BG139100 Rh7CG142800 Rh7DG140400
rosa_wichuraiana	Rw5G021230 Rw5G021450 Rw7G011830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	233
AclWI	GGATC	2 cut(s)	185, 213
AcsI	RAATTY	1 cut(s)	303
AcuI	CTGAAG	1 cut(s)	35
AcyI	GRCGYC	1 cut(s)	161
AfiI	CCNNNNNNNGG	1 cut(s)	233
AgsI	TTSAA	2 cut(s)	8, 541
AluBI	AGCT	2 cut(s)	257, 404
AluI	AGCT	2 cut(s)	257, 404
Alw26I	GTCTC	1 cut(s)	336
AlwI	GGATC	2 cut(s)	185, 213
ApeKI	GCWGC	3 cut(s)	272, 352, 355
ApoI	RAATTY	1 cut(s)	303
AseI	ATTAAT	1 cut(s)	429
AsuHPI	GGTGA	1 cut(s)	16
BbvI	GCAGC	3 cut(s)	284, 364, 367
BccI	CCATC	1 cut(s)	110
BceAI	ACGGC	1 cut(s)	260
BclI	TGATCA	1 cut(s)	57
BcoDI	GTCTC	1 cut(s)	336
BfmI	CTRYAG	1 cut(s)	399
BisI	GCNGC	3 cut(s)	273, 353, 356
BlsI	GCNGC	3 cut(s)	274, 354, 357
BmsI	GCATC	1 cut(s)	96
BsaHI	GRCGYC	1 cut(s)	161
BsaJI	CCNNGG	1 cut(s)	201
BsaXI	ACNNNNNCTCC	2 cut(s)	16, 46
Bsc4I	CCNNNNNNNGG	1 cut(s)	233
Bse3DI	GCAATG	1 cut(s)	123
BseDI	CCNNGG	1 cut(s)	201
BseLI	CCNNNNNNNGG	1 cut(s)	233
BseMI	GCAATG	1 cut(s)	123
BseRI	GAGGAG	1 cut(s)	190
BseXI	GCAGC	3 cut(s)	284, 364, 367
BsgI	GTGCAG	1 cut(s)	258
BslI	CCNNNNNNNGG	1 cut(s)	233
BsmAI	GTCTC	1 cut(s)	336
BsmBI	CGTCTC	1 cut(s)	336
BsmI	GAATGC	1 cut(s)	95
Bsp143I	GATC	4 cut(s)	57, 117, 190, 205
BspPI	GGATC	2 cut(s)	185, 213
BspQI	GCTCTTC	1 cut(s)	539
BsrDI	GCAATG	1 cut(s)	123
BssECI	CCNNGG	1 cut(s)	201
BssMI	GATC	4 cut(s)	57, 117, 190, 205
BssNI	GRCGYC	1 cut(s)	161
Bst6I	CTCTTC	3 cut(s)	51, 334, 539
BstACI	GRCGYC	1 cut(s)	161
BstKTI	GATC	4 cut(s)	60, 120, 193, 208
BstMAI	GTCTC	1 cut(s)	336
BstMBI	GATC	4 cut(s)	57, 117, 190, 205
BstMWI	GCNNNNNNNGC	1 cut(s)	555
BstSFI	CTRYAG	1 cut(s)	399
BstV1I	GCAGC	3 cut(s)	284, 364, 367
CseI	GACGC	1 cut(s)	169
CviAII	CATG	3 cut(s)	106, 454, 498
CviJI	RGCY	7 cut(s)	11, 200, 257, 275, 404, 414, 549
CviKI_1	RGCY	7 cut(s)	11, 200, 257, 275, 404, 414, 549
DpnI	GATC	4 cut(s)	59, 119, 192, 207
DpnII	GATC	4 cut(s)	57, 117, 190, 205
Eam1104I	CTCTTC	3 cut(s)	51, 334, 539
EarI	CTCTTC	3 cut(s)	51, 334, 539
Eco57I	CTGAAG	1 cut(s)	35
Esp3I	CGTCTC	1 cut(s)	336
FaeI	CATG	3 cut(s)	109, 457, 501
FatI	CATG	3 cut(s)	105, 453, 497
FbaI	TGATCA	1 cut(s)	57
Fnu4HI	GCNGC	3 cut(s)	273, 353, 356
Fsp4HI	GCNGC	3 cut(s)	273, 353, 356
GluI	GCNGC	3 cut(s)	273, 353, 356
HgaI	GACGC	1 cut(s)	169
Hin1I	GRCGYC	1 cut(s)	161
Hin1II	CATG	3 cut(s)	109, 457, 501
HinfI	GANTC	1 cut(s)	311
HphI	GGTGA	1 cut(s)	16
Hpy188III	TCNNGA	2 cut(s)	188, 194
HpyAV	CCTTC	2 cut(s)	10, 290
HpyCH4IV	ACGT	2 cut(s)	138, 509
HpyCH4V	TGCA	5 cut(s)	87, 239, 435, 453, 501
HpyF10VI	GCNNNNNNNGC	1 cut(s)	555
HpySE526I	ACGT	2 cut(s)	138, 509
Hsp92I	GRCGYC	1 cut(s)	161
Hsp92II	CATG	3 cut(s)	109, 457, 501
Ksp22I	TGATCA	1 cut(s)	57
Kzo9I	GATC	4 cut(s)	57, 117, 190, 205
LguI	GCTCTTC	1 cut(s)	539
LpnPI	CCDG	3 cut(s)	26, 48, 201
Lsp1109I	GCAGC	3 cut(s)	284, 364, 367
LweI	GCATC	1 cut(s)	96
MaeII	ACGT	2 cut(s)	138, 509
MaeIII	GTNAC	1 cut(s)	22
MalI	GATC	4 cut(s)	59, 119, 192, 207
MboI	GATC	4 cut(s)	57, 117, 190, 205
MboII	GAAGA	4 cut(s)	38, 351, 553, 556
MfeI	CAATTG	2 cut(s)	476, 520
MluCI	AATT	9 cut(s)	69, 215, 286, 303, 426, 430, 476, 520, 571
MlyI	GAGTC	1 cut(s)	305
MnlI	CCTC	9 cut(s)	42, 140, 168, 196, 285, 316, 325, 451, 505
MseI	TTAA	1 cut(s)	429
MunI	CAATTG	2 cut(s)	476, 520
Mva1269I	GAATGC	1 cut(s)	95
MwoI	GCNNNNNNNGC	1 cut(s)	555
NdeII	GATC	4 cut(s)	57, 117, 190, 205
NlaIII	CATG	3 cut(s)	109, 457, 501
NmeAIII	GCCGAG	1 cut(s)	226
NmuCI	GTSAC	1 cut(s)	22
PciSI	GCTCTTC	1 cut(s)	539
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PctI	GAATGC	1 cut(s)	95
PflMI	CCANNNNNTGG	1 cut(s)	233
PkrI	GCNGC	3 cut(s)	274, 354, 357
PleI	GAGTC	1 cut(s)	305
PpsI	GAGTC	1 cut(s)	305
PshBI	ATTAAT	1 cut(s)	429
SapI	GCTCTTC	1 cut(s)	539
SaqAI	TTAA	1 cut(s)	429
SatI	GCNGC	3 cut(s)	273, 353, 356
Sau3AI	GATC	4 cut(s)	57, 117, 190, 205
SchI	GAGTC	1 cut(s)	305
SetI	ASST	8 cut(s)	21, 34, 141, 259, 327, 400, 406, 512
SfaNI	GCATC	1 cut(s)	96
SfcI	CTRYAG	1 cut(s)	399
Sse9I	AATT	9 cut(s)	69, 215, 286, 303, 426, 430, 476, 520, 571
TaiI	ACGT	2 cut(s)	141, 512
TaqI	TCGA	1 cut(s)	314
TasI	AATT	9 cut(s)	69, 215, 286, 303, 426, 430, 476, 520, 571
Tru1I	TTAA	1 cut(s)	429
Tru9I	TTAA	1 cut(s)	429
TseFI	GTSAC	1 cut(s)	22
TseI	GCWGC	3 cut(s)	272, 352, 355
Tsp45I	GTSAC	1 cut(s)	22
TspDTI	ATGAA	2 cut(s)	272, 542
Van91I	CCANNNNNTGG	1 cut(s)	233
VspI	ATTAAT	1 cut(s)	429
XapI	RAATTY	1 cut(s)	303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.