Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003546

Physical Location & Seq

Genomic Coordinates Reverse (-)

35531380 .. 35531985

Gene Structure 606 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1244400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 606 bp

ATGGCTTCCCCACATGACCTCCCAATGGACAATCAAAGTTTGTTCACACAACGAATGGTCCTCGTGTACAACATCTTTTGTGAAGCCTTCGTTTCAGAACCCGATCCTCCAGAGCTTCCAGCTAAGATCATTGCATGCATCTCAATCGTCACCTTCTTGTTCTTGATTTGCAACCACATTCTCAATGTGACCCTTGCCTGGCTCCTAGGAGTCGTCCCAGAACATGATCTTGAGGGGGGAGACATCATTTACGGGCCTCGAATTGACATTCTCAATTCGAGTCCCTTTCGGATAGCTATAATGCAAAATACGCAGAGGCTGGGAGTGATGACGAGAGTCATGCAGGATTTCGATGAAGGAAGAGGGCCGAGGCTTAGGGCTTTGAAGAAATTGCCACCATTACTGAATTATGGAAGCTGTGAAATAAGATCAACGAGTTCATCAAGGTGTACTGCATGTGCAATTTGTTTGGAGGATTTTGCAGATGGTGATTCGTGTCAAATTTTTTCACTTTGCAACCACATGTTCCATTCGAATTGCATCGACCATTGGTTGATGAACAAGCCAACTTGCCCAGTTTGTCGAAATTGTATCATGGATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

202

Amino Acids

22.6

Weight (kDa)

5.42

Isoelectric Point (pI)

51.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	152 - 195	1.1e-13	Ring finger domain
zf-RING_11	PF17123	153 - 181	4.4e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	153 - 194	1.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	153 - 194	4.1e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	154 - 195	7.3e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17450 AT4G35480
fragaria_vesca	FvH4_3g20200 FvH4_5g05151
malus_domestica	MD03G1226600.v1.1 MD03G1226700.v1.1 MD06G1176900.v1.1 MD11G1244200.v1.1 MD11G1244300.v1.1 MD11G1244400.v1.1 MD14G1183100.v1.1
prunus_persica	Prupe.4G182700_v2.0.a1 Prupe.4G182800_v2.0.a1 Prupe.5G175600_v2.0.a1 Prupe.6G077100_v2.0.a1
pyrus_communis	pycom06g15700
rosa_chinensis	RchiOBHm_Chr5g0034021 RchiOBHm_Chr7g0193811
rosa_laevigata	RLG00000004243
rosa_multiflora	Rmu_sc0001436.1_g000029
rosa_roxburghii	Rroxscaffold_1G00046210 Rroxscaffold_3G00261810
rosa_rugosa	Rorug07G0012200
rosa_samantha	Rh5AG235200 Rh5BG235900 Rh5CG265700 Rh5CG265800 Rh5DG242400 Rh7AG138800 Rh7BG139100 Rh7CG142800 Rh7DG140400
rosa_wichuraiana	Rw5G021230 Rw5G021450 Rw7G011830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	98
AcsI	RAATTY	1 cut(s)	501
AfaI	GTAC	2 cut(s)	68, 451
AfiI	CCNNNNNNNGG	2 cut(s)	25, 198
AflIII	ACRYGT	1 cut(s)	522
AgsI	TTSAA	1 cut(s)	385
AjnI	CCWGG	1 cut(s)	197
AluBI	AGCT	4 cut(s)	115, 122, 296, 417
AluI	AGCT	4 cut(s)	115, 122, 296, 417
Alw26I	GTCTC	1 cut(s)	234
AlwI	GGATC	1 cut(s)	98
AlwNI	CAGNNNCTG	1 cut(s)	319
AoxI	GGCC	2 cut(s)	254, 365
ApoI	RAATTY	1 cut(s)	501
AspA2I	CCTAGG	1 cut(s)	205
AspS9I	GGNCC	3 cut(s)	58, 254, 365
AsuHPI	GGTGA	2 cut(s)	142, 500
AsuII	TTCGAA	1 cut(s)	533
AvaII	GGWCC	1 cut(s)	58
AvrII	CCTAGG	1 cut(s)	205
BauI	CACGAG	1 cut(s)	62
BccI	CCATC	1 cut(s)	479
BcgI	CGANNNNNNTGC	4 cut(s)	127, 161, 322, 356
BciT130I	CCWGG	1 cut(s)	199
BcoDI	GTCTC	1 cut(s)	234
BfaI	CTAG	1 cut(s)	206
BlnI	CCTAGG	1 cut(s)	205
Bme1390I	CCNGG	1 cut(s)	199
Bme18I	GGWCC	1 cut(s)	58
BmgT120I	GGNCC	3 cut(s)	58, 254, 365
BmiI	GGNNCC	1 cut(s)	203
BmrFI	CCNGG	1 cut(s)	199
BmrI	ACTGGG	1 cut(s)	569
BmsI	GCATC	2 cut(s)	147, 549
BmuI	ACTGGG	1 cut(s)	569
BoxI	GACNNNNGTC	1 cut(s)	335
BpmI	CTGGAG	1 cut(s)	93
Bpu10I	CCTNAGC	1 cut(s)	374
Bpu14I	TTCGAA	1 cut(s)	533
BpuEI	CTTGAG	1 cut(s)	251
BsaJI	CCNNGG	2 cut(s)	205, 368
BsaXI	ACNNNNNCTCC	1 cut(s)	33
Bsc4I	CCNNNNNNNGG	2 cut(s)	25, 198
Bse1I	ACTGG	1 cut(s)	575
Bse3DI	GCAATG	1 cut(s)	129
BseBI	CCWGG	1 cut(s)	199
BseDI	CCNNGG	2 cut(s)	205, 368
BseGI	GGATG	1 cut(s)	604
BseLI	CCNNNNNNNGG	2 cut(s)	25, 198
BseMI	GCAATG	1 cut(s)	129
BseNI	ACTGG	1 cut(s)	575
BseYI	CCCAGC	1 cut(s)	319
BshFI	GGCC	2 cut(s)	256, 367
BslFI	GGGAC	2 cut(s)	200, 267
BslI	CCNNNNNNNGG	2 cut(s)	25, 198
BsmAI	GTCTC	1 cut(s)	234
BsmFI	GGGAC	2 cut(s)	200, 267
BsnI	GGCC	2 cut(s)	256, 367
Bsp119I	TTCGAA	1 cut(s)	533
Bsp1407I	TGTACA	1 cut(s)	66
Bsp143I	GATC	4 cut(s)	103, 126, 226, 428
BspANI	GGCC	2 cut(s)	256, 367
BspLI	GGNNCC	1 cut(s)	203
BspPI	GGATC	1 cut(s)	98
BspT104I	TTCGAA	1 cut(s)	533
BsrDI	GCAATG	1 cut(s)	129
BsrGI	TGTACA	1 cut(s)	66
BsrI	ACTGG	1 cut(s)	575
BssECI	CCNNGG	2 cut(s)	205, 368
BssMI	GATC	4 cut(s)	103, 126, 226, 428
BssSI	CACGAG	1 cut(s)	62
BssT1I	CCWWGG	1 cut(s)	205
Bst2BI	CACGAG	1 cut(s)	62
Bst2UI	CCWGG	1 cut(s)	199
Bst6I	CTCTTC	1 cut(s)	355
BstAUI	TGTACA	1 cut(s)	66
BstBI	TTCGAA	1 cut(s)	533
BstC8I	GCNNGC	1 cut(s)	136
BstDEI	CTNAG	2 cut(s)	123, 374
BstF5I	GGATG	1 cut(s)	604
BstKTI	GATC	4 cut(s)	106, 129, 229, 431
BstMAI	GTCTC	1 cut(s)	234
BstMBI	GATC	4 cut(s)	103, 126, 226, 428
BstMWI	GCNNNNNNNGC	1 cut(s)	310
BstNI	CCWGG	1 cut(s)	199
BstNSI	RCATGY	3 cut(s)	138, 459, 526
BstPAI	GACNNNNGTC	1 cut(s)	335
BstSCI	CCNGG	1 cut(s)	197
BsuRI	GGCC	2 cut(s)	256, 367
BtsCI	GGATG	1 cut(s)	604
Cac8I	GCNNGC	1 cut(s)	136
CaiI	CAGNNNCTG	1 cut(s)	319
Cfr13I	GGNCC	3 cut(s)	58, 254, 365
Csp6I	GTAC	2 cut(s)	67, 450
CviAII	CATG	7 cut(s)	14, 135, 224, 340, 456, 523, 595
CviQI	GTAC	2 cut(s)	67, 450
DdeI	CTNAG	2 cut(s)	123, 374
DpnI	GATC	4 cut(s)	105, 128, 228, 430
DpnII	GATC	4 cut(s)	103, 126, 226, 428
Eam1104I	CTCTTC	1 cut(s)	355
EarI	CTCTTC	1 cut(s)	355
Eco130I	CCWWGG	1 cut(s)	205
Eco47I	GGWCC	1 cut(s)	58
EcoRII	CCWGG	1 cut(s)	197
EcoT14I	CCWWGG	1 cut(s)	205
EcoT22I	ATGCAT	1 cut(s)	140
ErhI	CCWWGG	1 cut(s)	205
FaeI	CATG	7 cut(s)	17, 138, 227, 343, 459, 526, 598
FaqI	GGGAC	2 cut(s)	200, 267
FatI	CATG	7 cut(s)	13, 134, 223, 339, 455, 522, 594
FspBI	CTAG	1 cut(s)	206
GsaI	CCCAGC	1 cut(s)	323
GsuI	CTGGAG	1 cut(s)	93
HaeIII	GGCC	2 cut(s)	256, 367
Hin1II	CATG	7 cut(s)	17, 138, 227, 343, 459, 526, 598
HinfI	GANTC	4 cut(s)	210, 280, 336, 491
HphI	GGTGA	2 cut(s)	142, 500
Hpy166II	GTNNAC	3 cut(s)	45, 67, 450
Hpy188I	TCNGA	2 cut(s)	97, 291
Hpy188III	TCNNGA	3 cut(s)	110, 163, 230
Hpy8I	GTNNAC	3 cut(s)	45, 67, 450
HpyAV	CCTTC	3 cut(s)	97, 163, 350
HpyF10VI	GCNNNNNNNGC	1 cut(s)	310
HpyF3I	CTNAG	2 cut(s)	123, 374
Hsp92II	CATG	7 cut(s)	17, 138, 227, 343, 459, 526, 598
Kzo9I	GATC	4 cut(s)	103, 126, 226, 428
LmnI	GCTCC	1 cut(s)	207
LpnPI	CCDG	8 cut(s)	123, 132, 184, 211, 231, 305, 329, 588
LweI	GCATC	2 cut(s)	147, 549
MaeI	CTAG	1 cut(s)	206
MaeIII	GTNAC	2 cut(s)	148, 187
MalI	GATC	4 cut(s)	105, 128, 228, 430
MboI	GATC	4 cut(s)	103, 126, 226, 428
MboII	GAAGA	2 cut(s)	372, 397
MluCI	AATT	8 cut(s)	261, 274, 389, 406, 462, 501, 535, 586
MlyI	GAGTC	3 cut(s)	219, 289, 345
MnlI	CCTC	9 cut(s)	29, 71, 117, 226, 267, 309, 356, 363, 466
Mph1103I	ATGCAT	1 cut(s)	140
MslI	CAYNNNNRTG	1 cut(s)	445
MspR9I	CCNGG	1 cut(s)	199
MvaI	CCWGG	1 cut(s)	199
MwoI	GCNNNNNNNGC	1 cut(s)	310
NdeII	GATC	4 cut(s)	103, 126, 226, 428
NlaIII	CATG	7 cut(s)	17, 138, 227, 343, 459, 526, 598
NlaIV	GGNNCC	1 cut(s)	203
NmeAIII	GCCGAG	1 cut(s)	393
NmuCI	GTSAC	2 cut(s)	148, 187
NsiI	ATGCAT	1 cut(s)	140
NspI	RCATGY	3 cut(s)	138, 459, 526
NspV	TTCGAA	1 cut(s)	533
PaeI	GCATGC	1 cut(s)	138
PciI	ACATGT	1 cut(s)	522
PfeI	GAWTC	1 cut(s)	491
PleI	GAGTC	3 cut(s)	218, 288, 344
PpsI	GAGTC	3 cut(s)	218, 288, 344
PscI	ACATGT	1 cut(s)	522
PshAI	GACNNNNGTC	1 cut(s)	335
Psp6I	CCWGG	1 cut(s)	197
PspFI	CCCAGC	1 cut(s)	319
PspGI	CCWGG	1 cut(s)	197
PspN4I	GGNNCC	1 cut(s)	203
PspPI	GGNCC	3 cut(s)	58, 254, 365
PstNI	CAGNNNCTG	1 cut(s)	319
RsaI	GTAC	2 cut(s)	68, 451
RsaNI	GTAC	2 cut(s)	67, 450
RseI	CAYNNNNRTG	1 cut(s)	445
Sau3AI	GATC	4 cut(s)	103, 126, 226, 428
Sau96I	GGNCC	3 cut(s)	58, 254, 365
SchI	GAGTC	3 cut(s)	219, 289, 345
ScrFI	CCNGG	1 cut(s)	199
SetI	ASST	7 cut(s)	21, 117, 124, 155, 298, 419, 449
SfaNI	GCATC	2 cut(s)	147, 549
SfuI	TTCGAA	1 cut(s)	533
SinI	GGWCC	1 cut(s)	58
SmiMI	CAYNNNNRTG	1 cut(s)	445
SmlI	CTYRAG	1 cut(s)	230
SmoI	CTYRAG	1 cut(s)	230
SphI	GCATGC	1 cut(s)	138
Sse9I	AATT	8 cut(s)	261, 274, 389, 406, 462, 501, 535, 586
SspMI	CTAG	1 cut(s)	206
StyD4I	CCNGG	1 cut(s)	197
StyI	CCWWGG	1 cut(s)	205
TaqI	TCGA	6 cut(s)	259, 278, 351, 533, 543, 583
TasI	AATT	8 cut(s)	261, 274, 389, 406, 462, 501, 535, 586
TatI	WGTACW	2 cut(s)	66, 449
TfiI	GAWTC	1 cut(s)	491
TseFI	GTSAC	2 cut(s)	148, 187
Tsp45I	GTSAC	2 cut(s)	148, 187
TspDTI	ATGAA	3 cut(s)	369, 429, 572
VpaK11BI	GGWCC	1 cut(s)	58
XapI	RAATTY	1 cut(s)	501
XceI	RCATGY	3 cut(s)	138, 459, 526
XmaJI	CCTAGG	1 cut(s)	205
XspI	CTAG	1 cut(s)	206
Zsp2I	ATGCAT	1 cut(s)	140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.