AT5G08350

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003141
Physical Location & Seq
Reverse (-)
2685712 .. 2687476
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08350.1

Sequence Viewer

Length: 669 bp
ATGAACATGAGCAGAGTTGAACAACAAGTCATTAGATATCCGGCCGCAAAGGCTACTCCCGTGGGTTACTTACCTGATCCGGCTTCATTTAACAAGTTCCGAGTTCCGGCTTCTTCCAAGAAATCTGAACAAAGCAACGTAAAATCGATTCTTAAAAGGAAGAAAACTGATGGGTTCACCAACGGAGTTAGAGACCAGAGCAAGATAAGACCAAAGCTAACCGAAACTGTGAAGAGAAAGCTATCTTTGGGCGCTAGGATTCTTCAGGTTGGAGGTTTAGAGAAGATCTTCAAGCGGCTCTTTAGAGTTAGTGAAGGTGAGAAACTGTTCAAAATGTACCAGTGTTACTTGTCGACAACCGCGGGTCCCATAGCCGGCCTTCTCTTCATCTCTTCCAAGAAAATGGCGTTCTGCAGCGAGAGATCAATAAAGGTGGATTCCCCTCAAGGAGACATAATCCGGGTTCACTACAAAGTATCAATCCCCTTGTGCAAGATCGACAGAGTGAACCAGAGTCAGAACACAAAGAAACCATCACAGAAGTACCTAGAAGTAGTCACGGTCGATGGTTTTGACTTCTGGTTCATGGGGTTCTTGAGCTACCAGAAAGCTTTCAATTGCCTTGAGAAGGCTCTTTCACTGAGCTTTGAAGATAACAAAGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

222

Amino Acids

25.25

Weight (kDa)

9.91

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 96 - 213 1.5e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 362
AciI CCGC 4 cut(s) 45, 295, 360, 362
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 2 cut(s) 338, 545
AfiI CCNNNNNNNGG 3 cut(s) 106, 374, 628
AgsI TTSAA 5 cut(s) 20, 292, 331, 616, 650
AluBI AGCT 5 cut(s) 217, 241, 600, 611, 645
AluI AGCT 5 cut(s) 217, 241, 600, 611, 645
Alw26I GTCTC 2 cut(s) 186, 444
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 42, 376
ApeKI GCWGC 1 cut(s) 414
Asp700I GAANNNNTTC 3 cut(s) 287, 326, 611
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 2 cut(s) 169, 329
AvaII GGWCC 1 cut(s) 365
BaeI ACNNNNGTAYC 2 cut(s) 527, 560
BbvI GCAGC 1 cut(s) 426
BccI CCATC 3 cut(s) 164, 541, 560
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 2 cut(s) 186, 444
BfaI CTAG 2 cut(s) 255, 548
BfmI CTRYAG 1 cut(s) 412
BfoI RGCGCY 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 1 cut(s) 285
BisI GCNGC 3 cut(s) 45, 296, 415
BlsI GCNGC 3 cut(s) 46, 297, 416
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 2 cut(s) 366, 367
BmrFI CCNGG 1 cut(s) 461
BpuEI CTTGAG 3 cut(s) 429, 616, 644
BpuMI CCSGG 1 cut(s) 461
Bsa29I ATCGAT 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 60, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 374, 628
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 340
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 60, 360
BseLI CCNNNNNNNGG 3 cut(s) 106, 374, 628
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 1 cut(s) 340
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 2 cut(s) 45, 564
BshFI GGCC 2 cut(s) 44, 378
BshVI ATCGAT 1 cut(s) 146
BsiEI CGRYCG 2 cut(s) 45, 564
BsiSI CCGG 5 cut(s) 41, 80, 107, 375, 460
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 106, 374, 628
BsmAI GTCTC 2 cut(s) 186, 444
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 2 cut(s) 44, 378
Bso31I GGTCTC 1 cut(s) 186
Bsp143I GATC 4 cut(s) 76, 285, 422, 495
BspACI CCGC 4 cut(s) 45, 295, 360, 362
BspANI GGCC 2 cut(s) 44, 378
BspCNI CTCAG 1 cut(s) 633
BspDI ATCGAT 1 cut(s) 146
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 2 cut(s) 366, 367
BspMAI CTGCAG 1 cut(s) 416
BspPI GGATC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 60, 360
BssMI GATC 4 cut(s) 76, 285, 422, 495
Bst4CI ACNGT 3 cut(s) 229, 327, 562
Bst6I CTCTTC 3 cut(s) 227, 389, 397
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 1 cut(s) 641
BstDSI CCRYGG 2 cut(s) 60, 360
BstENI CCTNNNNNAGG 1 cut(s) 626
BstFNI CGCG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 4 cut(s) 79, 288, 425, 498
BstMAI GTCTC 2 cut(s) 186, 444
BstMBI GATC 4 cut(s) 76, 285, 422, 495
BstMCI CGRYCG 2 cut(s) 45, 564
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 459
BstSFI CTRYAG 1 cut(s) 412
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 285
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 1 cut(s) 285
BstZI CGGCCG 1 cut(s) 42
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 2 cut(s) 44, 378
BsuTUI ATCGAT 1 cut(s) 146
BtgI CCRYGG 2 cut(s) 60, 360
BtsIMutI CAGTG 2 cut(s) 347, 638
Cac8I GCNNGC 1 cut(s) 376
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 365
Cfr42I CCGCGG 1 cut(s) 363
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 2 cut(s) 337, 544
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviAII CATG 2 cut(s) 7, 586
CviQI GTAC 2 cut(s) 337, 544
DdeI CTNAG 1 cut(s) 641
DpnI GATC 4 cut(s) 78, 287, 424, 497
DpnII GATC 4 cut(s) 76, 285, 422, 495
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 3 cut(s) 227, 389, 397
EarI CTCTTC 3 cut(s) 227, 389, 397
EclXI CGGCCG 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 365
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 365
EcoRV GATATC 1 cut(s) 38
FaeI CATG 2 cut(s) 10, 589
FaiI YATR 4 cut(s) 8, 371, 455, 587
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 1 cut(s) 351
FatI CATG 2 cut(s) 6, 585
FauI CCCGC 1 cut(s) 355
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 3 cut(s) 45, 296, 415
Fsp4HI GCNGC 3 cut(s) 45, 296, 415
FspBI CTAG 2 cut(s) 255, 548
GlaI GCGC 1 cut(s) 253
GluI GCNGC 3 cut(s) 45, 296, 415
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 2 cut(s) 44, 378
HapII CCGG 5 cut(s) 41, 80, 107, 375, 460
HhaI GCGC 1 cut(s) 254
Hin1II CATG 2 cut(s) 10, 589
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 4 cut(s) 148, 259, 437, 514
HpaII CCGG 5 cut(s) 41, 80, 107, 375, 460
HphI GGTGA 2 cut(s) 169, 329
Hpy166II GTNNAC 4 cut(s) 177, 354, 466, 508
Hpy188I TCNGA 3 cut(s) 101, 127, 519
Hpy188III TCNNGA 1 cut(s) 595
Hpy8I GTNNAC 4 cut(s) 177, 354, 466, 508
HpyAV CCTTC 3 cut(s) 308, 389, 622
HpyCH4III ACNGT 3 cut(s) 229, 327, 562
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 414, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 641
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 2 cut(s) 10, 589
HspAI GCGC 1 cut(s) 252
KflI GGGWCCC 1 cut(s) 365
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
KspI CCGCGG 1 cut(s) 363
Kzo9I GATC 4 cut(s) 76, 285, 422, 495
Lsp1109I GCAGC 1 cut(s) 426
MaeI CTAG 2 cut(s) 255, 548
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 3 cut(s) 65, 344, 556
MalI GATC 4 cut(s) 78, 287, 424, 497
MboI GATC 4 cut(s) 76, 285, 422, 495
MboII GAAGA 9 cut(s) 105, 172, 244, 254, 280, 295, 376, 384, 662
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 1 cut(s) 285
MluCI AATT 1 cut(s) 616
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 2 cut(s) 266, 453
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 3 cut(s) 287, 326, 611
MseI TTAA 2 cut(s) 90, 153
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 5 cut(s) 41, 80, 107, 375, 460
MspR9I CCNGG 1 cut(s) 461
MunI CAATTG 1 cut(s) 616
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 1 cut(s) 461
NdeII GATC 4 cut(s) 76, 285, 422, 495
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 2 cut(s) 10, 589
NlaIV GGNNCC 2 cut(s) 366, 367
NmuCI GTSAC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 3 cut(s) 287, 326, 611
PfeI GAWTC 3 cut(s) 148, 259, 437
PkrI GCNGC 3 cut(s) 46, 297, 416
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
PpuMI RGGWCCY 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 365
PspN4I GGNNCC 2 cut(s) 366, 367
PspPI GGNCC 1 cut(s) 365
PspPPI RGGWCCY 1 cut(s) 365
PstI CTGCAG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 2 cut(s) 338, 545
RsaNI GTAC 2 cut(s) 337, 544
SacII CCGCGG 1 cut(s) 363
SalI GTCGAC 1 cut(s) 352
SaqAI TTAA 2 cut(s) 90, 153
SatI GCNGC 3 cut(s) 45, 296, 415
Sau3AI GATC 4 cut(s) 76, 285, 422, 495
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 461
SfcI CTRYAG 1 cut(s) 412
Sfr303I CCGCGG 1 cut(s) 363
SgrBI CCGCGG 1 cut(s) 363
SinI GGWCC 1 cut(s) 365
SmlI CTYRAG 3 cut(s) 444, 595, 623
SmoI CTYRAG 3 cut(s) 444, 595, 623
Sse9I AATT 1 cut(s) 616
SsiI CCGC 4 cut(s) 45, 295, 360, 362
SspMI CTAG 2 cut(s) 255, 548
StyD4I CCNGG 1 cut(s) 459
TaaI ACNGT 3 cut(s) 229, 327, 562
TaiI ACGT 1 cut(s) 141
TaqI TCGA 4 cut(s) 146, 353, 498, 564
TasI AATT 1 cut(s) 616
TauI GCSGC 2 cut(s) 47, 298
TfiI GAWTC 3 cut(s) 148, 259, 437
Tru1I TTAA 2 cut(s) 90, 153
Tru9I TTAA 2 cut(s) 90, 153
TscAI CASTG 2 cut(s) 347, 645
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 17, 75, 376, 574
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 2 cut(s) 347, 645
VpaK11BI GGWCC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 3 cut(s) 287, 326, 611
XspI CTAG 2 cut(s) 255, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.