FvH4_4g37190

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003141
Physical Location & Seq
Reverse (-)
33770982 .. 33772396
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37190.t1

Sequence Viewer

Length: 672 bp
ATGAAGGCTTCATTTCAGGTTCAGCTTGGAGGACAAGTTATTAGTTTCCCAGTCAATTCAGCAGCAGCCGCAGCAGCAACATCATGCACATTTCAGAGGTCTCCAATTAATGCAAAGAGACTCCTACTACCTGCTCAGCATCATGTTCCCTCATCTGATCATCGTCGCGATCAAACTAGCCCTTCAAAACGGACACAAATGAAACTGGGGCCTAAAATTAGTGAGGCAGTGAAGGGAAAGTTGAGACTTGGGGCTAAGATTCTTAAAGCAGGTGGAACAATGGGGAAGGTGTTCAAGCATTTGTTTAGCAATAATGCTGAAGCAGCAGGAGAGAAGCTTTTGAAGACTTCCCACTGTTTTCTATCAACCACAGCAGGTCCTATTGCAGGCCTCCTCTTCATCTCCACACACAAGATTGCCTTCTGCAGTGACAAGCCAATCAAACTATCATCATCTCCAAATCCAAACGGAGAACTCATCAGAGTCCACTACAAGGTTGTGATCCCACTCAATAAGATAAACAGAGTAAACCAAAGTGAGGACGTGAAGAAGCCCTCGCAGAAGTACATAGAAATAGTTACTGTGGATAATTTTGACTTCTGGTTCATGGGTTTCTTCAATTACCAGAAAGCTCACAAATATCTCCAACAGGCATTAATTTCTCAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

224

Amino Acids

24.55

Weight (kDa)

10.1

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 98 - 219 8.7e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
Acc36I ACCTGC 3 cut(s) 139, 260, 365
AccII CGCG 1 cut(s) 168
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 3 cut(s) 386, 493, 538
AgsI TTSAA 4 cut(s) 186, 295, 343, 619
AjiI CACGTC 1 cut(s) 544
AluBI AGCT 3 cut(s) 25, 337, 632
AluI AGCT 3 cut(s) 25, 337, 632
Alw26I GTCTC 3 cut(s) 105, 112, 238
AlwI GGATC 1 cut(s) 496
AoxI GGCC 2 cut(s) 209, 388
ApeKI GCWGC 5 cut(s) 62, 65, 71, 74, 323
AseI ATTAAT 2 cut(s) 108, 656
Asp700I GAANNNNTTC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 209, 377
AvaII GGWCC 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 5 cut(s) 74, 77, 83, 86, 335
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 3 cut(s) 105, 112, 238
BfaI CTAG 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 424
BfuAI ACCTGC 3 cut(s) 139, 260, 365
BisI GCNGC 6 cut(s) 63, 66, 69, 72, 75, 324
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 6 cut(s) 64, 67, 70, 73, 76, 325
Bme18I GGWCC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 209, 377
BmiI GGNNCC 1 cut(s) 210
BmrI ACTGGG 2 cut(s) 44, 215
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 2 cut(s) 44, 215
BpiI GAAGAC 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 493, 538
Bse1I ACTGG 2 cut(s) 50, 210
BseLI CCNNNNNNNGG 3 cut(s) 386, 493, 538
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 2 cut(s) 50, 210
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 5 cut(s) 74, 77, 83, 86, 335
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 211, 390
BslI CCNNNNNNNGG 3 cut(s) 386, 493, 538
BsmAI GTCTC 3 cut(s) 105, 112, 238
BsnI GGCC 2 cut(s) 211, 390
Bso31I GGTCTC 1 cut(s) 105
Bsp143I GATC 3 cut(s) 157, 169, 501
Bsp1720I GCTNAGC 1 cut(s) 135
Bsp68I TCGCGA 1 cut(s) 168
BspACI CCGC 1 cut(s) 69
BspANI GGCC 2 cut(s) 211, 390
BspCNI CTCAG 1 cut(s) 148
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 428
BspMI ACCTGC 3 cut(s) 139, 260, 365
BspPI GGATC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 105
BsrI ACTGG 2 cut(s) 50, 210
BssMI GATC 3 cut(s) 157, 169, 501
Bst4CI ACNGT 2 cut(s) 356, 583
Bst6I CTCTTC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 135, 255, 663
BstENI CCTNNNNNAGG 1 cut(s) 384
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 3 cut(s) 160, 172, 504
BstMAI GTCTC 3 cut(s) 105, 112, 238
BstMBI GATC 3 cut(s) 157, 169, 501
BstMWI GCNNNNNNNGC 4 cut(s) 68, 71, 74, 323
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 5 cut(s) 74, 77, 83, 86, 335
BstV2I GAAGAC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 211, 390
BtrI CACGTC 1 cut(s) 544
BtsI GCAGTG 2 cut(s) 234, 433
BtsIMutI CAGTG 3 cut(s) 234, 352, 433
BtuMI TCGCGA 1 cut(s) 168
BveI ACCTGC 3 cut(s) 139, 260, 365
Cac8I GCNNGC 1 cut(s) 388
Cfr13I GGNCC 2 cut(s) 209, 377
Csp6I GTAC 1 cut(s) 565
CviAII CATG 3 cut(s) 84, 143, 607
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 3 cut(s) 135, 255, 663
DpnI GATC 3 cut(s) 159, 171, 503
DpnII GATC 3 cut(s) 157, 169, 501
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco147I AGGCCT 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoO109I RGGNCCY 2 cut(s) 209, 377
FaeI CATG 3 cut(s) 87, 146, 610
FaiI YATR 5 cut(s) 85, 144, 569, 608, 670
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FatI CATG 3 cut(s) 83, 142, 606
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 6 cut(s) 63, 66, 69, 72, 75, 324
Fsp4HI GCNGC 6 cut(s) 63, 66, 69, 72, 75, 324
FspBI CTAG 1 cut(s) 177
GluI GCNGC 6 cut(s) 63, 66, 69, 72, 75, 324
HaeIII GGCC 2 cut(s) 211, 390
Hin1II CATG 3 cut(s) 87, 146, 610
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 3 cut(s) 120, 259, 483
Hpy166II GTNNAC 2 cut(s) 487, 529
Hpy188I TCNGA 3 cut(s) 96, 157, 482
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 2 cut(s) 487, 529
Hpy99I CGWCG 1 cut(s) 168
HpyAV CCTTC 4 cut(s) 192, 226, 280, 430
HpyCH4III ACNGT 2 cut(s) 356, 583
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 4 cut(s) 87, 113, 386, 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 71, 74, 323
HpyF3I CTNAG 3 cut(s) 135, 255, 663
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 87, 146, 610
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 157, 169, 501
Lsp1109I GCAGC 5 cut(s) 74, 77, 83, 86, 335
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 2 cut(s) 428, 577
MalI GATC 3 cut(s) 159, 171, 503
MboI GATC 3 cut(s) 157, 169, 501
MboII GAAGA 4 cut(s) 355, 388, 559, 607
MluCI AATT 6 cut(s) 55, 105, 216, 589, 619, 657
MlyI GAGTC 2 cut(s) 114, 492
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 8 cut(s) 23, 90, 160, 217, 401, 404, 532, 565
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 3 cut(s) 108, 264, 656
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 68, 71, 74, 323
NdeII GATC 3 cut(s) 157, 169, 501
NlaIII CATG 3 cut(s) 87, 146, 610
NlaIV GGNNCC 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 428
NruI TCGCGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 260
PceI AGGCCT 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 6 cut(s) 64, 67, 70, 73, 76, 325
PleI GAGTC 2 cut(s) 114, 491
PpsI GAGTC 2 cut(s) 114, 491
PpuMI RGGWCCY 1 cut(s) 377
PshBI ATTAAT 2 cut(s) 108, 656
Psp5II RGGWCCY 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 2 cut(s) 209, 377
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 1 cut(s) 428
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
SaqAI TTAA 3 cut(s) 108, 264, 656
SatI GCNGC 6 cut(s) 63, 66, 69, 72, 75, 324
Sau3AI GATC 3 cut(s) 157, 169, 501
Sau96I GGNCC 2 cut(s) 209, 377
SchI GAGTC 2 cut(s) 114, 492
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 1 cut(s) 377
Sse9I AATT 6 cut(s) 55, 105, 216, 589, 619, 657
SseBI AGGCCT 1 cut(s) 390
SsiI CCGC 1 cut(s) 69
SspMI CTAG 1 cut(s) 177
StuI AGGCCT 1 cut(s) 390
TaaI ACNGT 2 cut(s) 356, 583
TaiI ACGT 1 cut(s) 546
TasI AATT 6 cut(s) 55, 105, 216, 589, 619, 657
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 3 cut(s) 108, 264, 656
Tru9I TTAA 3 cut(s) 108, 264, 656
TscAI CASTG 3 cut(s) 234, 359, 433
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 5 cut(s) 62, 65, 71, 74, 323
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 4 cut(s) 17, 215, 388, 595
TspGWI ACGGA 2 cut(s) 205, 483
TspRI CASTG 3 cut(s) 234, 359, 433
VpaK11BI GGWCC 1 cut(s) 377
VspI ATTAAT 2 cut(s) 108, 656
XagI CCTNNNNNAGG 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.