Rh4BG447000

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003141
Physical Location & Seq
Reverse (-)
58559416 .. 58560302
887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG447000.1

Sequence Viewer

Length: 684 bp
ATGAAGGCTTCATTTCAGGTTCAGCTTGGAGGACAAGTTCTTGGTTTCCCAATCAATTCAGCAGCAGCAGCATCATGCACATTTGAGAGGTCCCCTATGAATGCAAAGAGACTCCTGCTACCTGCTCAGCATCATGTTGCTATTCAGTATGATCATGTTCCATCATCTGATCATCGTCGTGATCAAATTGGGTCTTCAAAACGGACACAAATGAAACTGAGGCCTAAAATCAGTGAGACAGTGAAGGGAAAGTTGAGACTTGGGGCTAAGATTCTTAGAGCAGGTGGAACAATGGAGAAGGTGTTCAAGCATTTGTTTAGTAATAATGATAAAGCAGCAGGAGAGAAGCTTTTGAAGACTTCTCACTGTTATCTATCAACCACAGCAGGTCCTATTGCAGGCCTCCTTTTCATCTCCACACACAAGATTGCCTTCTGCAGTGACAAGCCAATCAAACTGTCATCATCGCCAAATCCAACTGGACAACTCATCAGAGTCCACTACAAGGTTGTGATCCCACTCAATAAGATAAACAGAGTAAACCAGAGTGAGGATGTGAAGAAGCCATCACAGAAGTACATAGAAATAGTTACCGTGGATAATTATGACTTCTGGTTTATGGGTTTCTTCAATTACCAGAAAGCTCACAAATATCTCCAACAGGCATTAATTTCTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

227

Amino Acids

25.4

Weight (kDa)

10.01

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 102 - 223 4.5e-16 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 272
Acc36I ACCTGC 3 cut(s) 130, 272, 377
AclWI GGATC 1 cut(s) 508
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 3 cut(s) 398, 505, 550
AgsI TTSAA 4 cut(s) 198, 307, 355, 631
AloI GAACNNNNNNTCC 4 cut(s) 21, 53, 287, 319
AluBI AGCT 3 cut(s) 25, 349, 644
AluI AGCT 3 cut(s) 25, 349, 644
Alw26I GTCTC 3 cut(s) 103, 230, 250
AlwI GGATC 1 cut(s) 508
AoxI GGCC 2 cut(s) 221, 400
ApeKI GCWGC 4 cut(s) 62, 65, 68, 335
AseI ATTAAT 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 90, 389
AvaII GGWCC 2 cut(s) 90, 389
BbsI GAAGAC 2 cut(s) 186, 362
BbvI GCAGC 4 cut(s) 74, 77, 80, 347
BccI CCATC 2 cut(s) 169, 574
BclI TGATCA 3 cut(s) 151, 169, 181
BcoDI GTCTC 3 cut(s) 103, 230, 250
BfmI CTRYAG 1 cut(s) 436
BfuAI ACCTGC 3 cut(s) 130, 272, 377
BisI GCNGC 4 cut(s) 63, 66, 69, 336
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 4 cut(s) 64, 67, 70, 337
Bme18I GGWCC 2 cut(s) 90, 389
BmgT120I GGNCC 2 cut(s) 90, 389
BmiI GGNNCC 1 cut(s) 92
BmsI GCATC 2 cut(s) 80, 139
BpiI GAAGAC 2 cut(s) 186, 362
Bpu1102I GCTNAGC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 594
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 505, 550
Bse1I ACTGG 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 398, 505, 550
BseMII CTCAG 2 cut(s) 140, 209
BseNI ACTGG 1 cut(s) 484
BseXI GCAGC 4 cut(s) 74, 77, 80, 347
BshFI GGCC 2 cut(s) 223, 402
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 3 cut(s) 398, 505, 550
BsmAI GTCTC 3 cut(s) 103, 230, 250
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 2 cut(s) 223, 402
Bsp143I GATC 4 cut(s) 151, 169, 181, 513
Bsp1720I GCTNAGC 1 cut(s) 126
BspANI GGCC 2 cut(s) 223, 402
BspCNI CTCAG 2 cut(s) 139, 210
BspLI GGNNCC 1 cut(s) 92
BspMAI CTGCAG 1 cut(s) 440
BspMI ACCTGC 3 cut(s) 130, 272, 377
BspPI GGATC 1 cut(s) 508
BsrI ACTGG 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 4 cut(s) 151, 169, 181, 513
Bst4CI ACNGT 4 cut(s) 241, 368, 459, 595
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 6 cut(s) 126, 218, 267, 275, 675, 681
BstDSI CCRYGG 1 cut(s) 594
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 559
BstKTI GATC 4 cut(s) 154, 172, 184, 516
BstMAI GTCTC 3 cut(s) 103, 230, 250
BstMBI GATC 4 cut(s) 151, 169, 181, 513
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 4 cut(s) 74, 77, 80, 347
BstV2I GAAGAC 2 cut(s) 186, 362
BsuRI GGCC 2 cut(s) 223, 402
BtgI CCRYGG 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 1 cut(s) 559
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 4 cut(s) 238, 246, 364, 445
BveI ACCTGC 3 cut(s) 130, 272, 377
Cac8I GCNNGC 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 90, 389
Csp6I GTAC 1 cut(s) 577
CviAII CATG 3 cut(s) 75, 134, 155
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 6 cut(s) 126, 218, 267, 275, 675, 681
DpnI GATC 4 cut(s) 153, 171, 183, 515
DpnII GATC 4 cut(s) 151, 169, 181, 513
Eco147I AGGCCT 2 cut(s) 223, 402
Eco47I GGWCC 2 cut(s) 90, 389
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 90, 389
FaeI CATG 3 cut(s) 78, 137, 158
FaiI YATR 8 cut(s) 76, 98, 135, 150, 156, 581, 606, 620
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 76
FatI CATG 3 cut(s) 74, 133, 154
FbaI TGATCA 3 cut(s) 151, 169, 181
Fnu4HI GCNGC 4 cut(s) 63, 66, 69, 336
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 4 cut(s) 63, 66, 69, 336
GluI GCNGC 4 cut(s) 63, 66, 69, 336
HaeIII GGCC 2 cut(s) 223, 402
Hin1II CATG 3 cut(s) 78, 137, 158
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 111, 271, 495
Hpy166II GTNNAC 2 cut(s) 499, 541
Hpy188I TCNGA 2 cut(s) 169, 494
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 2 cut(s) 499, 541
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 238, 292, 442
HpyCH4III ACNGT 4 cut(s) 241, 368, 459, 595
HpyCH4V TGCA 4 cut(s) 78, 104, 398, 438
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 6 cut(s) 126, 218, 267, 275, 675, 681
Hsp92II CATG 3 cut(s) 78, 137, 158
Ksp22I TGATCA 3 cut(s) 151, 169, 181
Kzo9I GATC 4 cut(s) 151, 169, 181, 513
Lsp1109I GCAGC 4 cut(s) 74, 77, 80, 347
LweI GCATC 2 cut(s) 80, 139
MaeIII GTNAC 2 cut(s) 440, 589
MalI GATC 4 cut(s) 153, 171, 183, 515
MboI GATC 4 cut(s) 151, 169, 181, 513
MboII GAAGA 4 cut(s) 186, 367, 571, 619
MluCI AATT 5 cut(s) 55, 186, 601, 631, 669
MlyI GAGTC 2 cut(s) 105, 504
MmeI TCCRAC 2 cut(s) 500, 682
MnlI CCTC 5 cut(s) 23, 81, 213, 413, 544
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 1 cut(s) 668
MslI CAYNNNNRTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 4 cut(s) 151, 169, 181, 513
NlaIII CATG 3 cut(s) 78, 137, 158
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 440
PaqCI CACCTGC 1 cut(s) 272
PceI AGGCCT 2 cut(s) 223, 402
PctI GAATGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 4 cut(s) 64, 67, 70, 337
PleI GAGTC 2 cut(s) 105, 503
PpsI GAGTC 2 cut(s) 105, 503
PpuMI RGGWCCY 2 cut(s) 90, 389
PshBI ATTAAT 1 cut(s) 668
Psp5II RGGWCCY 2 cut(s) 90, 389
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 90, 389
PspPPI RGGWCCY 2 cut(s) 90, 389
PstI CTGCAG 1 cut(s) 440
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 1 cut(s) 668
SatI GCNGC 4 cut(s) 63, 66, 69, 336
Sau3AI GATC 4 cut(s) 151, 169, 181, 513
Sau96I GGNCC 2 cut(s) 90, 389
SchI GAGTC 2 cut(s) 105, 504
SfaNI GCATC 2 cut(s) 80, 139
SfcI CTRYAG 1 cut(s) 436
SinI GGWCC 2 cut(s) 90, 389
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 5 cut(s) 55, 186, 601, 631, 669
SseBI AGGCCT 2 cut(s) 223, 402
StuI AGGCCT 2 cut(s) 223, 402
TaaI ACNGT 4 cut(s) 241, 368, 459, 595
TasI AATT 5 cut(s) 55, 186, 601, 631, 669
TatI WGTACW 1 cut(s) 576
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 668
Tru9I TTAA 1 cut(s) 668
TscAI CASTG 4 cut(s) 238, 246, 371, 445
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 4 cut(s) 62, 65, 68, 335
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 4 cut(s) 17, 113, 227, 400
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 4 cut(s) 238, 246, 371, 445
VpaK11BI GGWCC 2 cut(s) 90, 389
VspI ATTAAT 1 cut(s) 668
XagI CCTNNNNNAGG 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.