AT5G23370

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003141
Physical Location & Seq
Reverse (-)
7863216 .. 7864291
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23370.1

Sequence Viewer

Length: 660 bp
ATGACATTGAGCAGAGTTCACCAACAACTCATTACATTTCCTGCCGTCAAGACTTCTCCGGCGGGTTACTTGCCTGATCCAGCTTCCATCAACAAGCTTCAAATCCCAACTTCTTCAAAGTTTTCTTTTCTAACAGGCAAAGGAAAATCGATGCTACGAAAGAAGAAAAACGATAGCTTCACAAACGGAGTTAGAGATCAGGACAAGTTAGGACCAAAGCTAACCGAAACAGTCAAGAGAAAACTATCCTTGGGAGCTAGGATCCTTCAAATGGGAGGCTTAGAGAAGATCTATAAACGACTCTTTAAGGTCAGCGATGAAGAGAAACTCTTCAAGGCGTACCAATGCTACCTATCCACAACCGCAGGTCCCATCGCTGGCCTACTCTTCATATCATCAAAGAAAATTGCATTCTGCAGCGAAAGATCGATCAAGGTGGCTTCTCCTCAAGGAGAGCTCAATAGGGTTCACTACAAAGTCTCAATCCCCTTGTGCAAGATCAATGGAGTGAACCAGAGTCAAAACACAACGAAGCCATCTCAGAAGTACCTTGAAGTAGTCACGGTCGACGGCTTTGACTTCTGGTTCATGGGATTCTTGAGCTACCAAAAAGCTTTCAACTGCCTCGAGCAAGCGCTTTCTCTTAGCTTCAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

219

Amino Acids

24.55

Weight (kDa)

9.89

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 99 - 214 6.8e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 567
AciI CCGC 2 cut(s) 62, 363
AclWI GGATC 3 cut(s) 71, 256, 269
AfaI GTAC 2 cut(s) 341, 548
AfeI AGCGCT 1 cut(s) 636
AfiI CCNNNNNNNGG 2 cut(s) 271, 377
AgsI TTSAA 7 cut(s) 101, 117, 269, 334, 554, 619, 652
AluBI AGCT 9 cut(s) 83, 97, 177, 220, 257, 457, 603, 614, 648
AluI AGCT 9 cut(s) 83, 97, 177, 220, 257, 457, 603, 614, 648
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 1 cut(s) 484
AlwI GGATC 3 cut(s) 71, 256, 269
Ama87I CYCGRG 1 cut(s) 626
Aor51HI AGCGCT 1 cut(s) 636
AoxI GGCC 1 cut(s) 379
ApeKI GCWGC 1 cut(s) 417
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 212, 368
AsuHPI GGTGA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 2 cut(s) 212, 368
BamHI GGATCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 1 cut(s) 429
BccI CCATC 3 cut(s) 95, 380, 544
BceAI ACGGC 2 cut(s) 29, 586
BcoDI GTCTC 1 cut(s) 484
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 415
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 356
BglII AGATCT 1 cut(s) 288
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme18I GGWCC 2 cut(s) 212, 368
BmeT110I CYCGRG 1 cut(s) 626
BmgT120I GGNCC 2 cut(s) 212, 368
BmiI GGNNCC 2 cut(s) 263, 370
BmsI GCATC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 432, 619
Bsa29I ATCGAT 2 cut(s) 149, 428
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 377
BseCI ATCGAT 2 cut(s) 149, 428
BseDI CCNNGG 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 271, 377
BseMII CTCAG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 381
BshVI ATCGAT 2 cut(s) 149, 428
BsiEI CGRYCG 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 626
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 271, 377
BsmAI GTCTC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 1 cut(s) 381
BsoBI CYCGRG 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
BspACI CCGC 2 cut(s) 62, 363
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 1 cut(s) 553
BspDI ATCGAT 2 cut(s) 149, 428
BspLI GGNNCC 2 cut(s) 263, 370
BspMAI CTGCAG 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 3 cut(s) 71, 256, 269
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 232, 565
Bst6I CTCTTC 3 cut(s) 315, 335, 392
BstC8I GCNNGC 2 cut(s) 379, 633
BstDEI CTNAG 3 cut(s) 280, 540, 644
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 7 cut(s) 79, 199, 264, 291, 428, 432, 501
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
BstMCI CGRYCG 1 cut(s) 567
BstSFI CTRYAG 1 cut(s) 415
BstV1I GCAGC 1 cut(s) 429
BstX2I RGATCY 2 cut(s) 261, 288
BstYI RGATCY 2 cut(s) 261, 288
Bsu15I ATCGAT 2 cut(s) 149, 428
BsuRI GGCC 1 cut(s) 381
BsuTUI ATCGAT 2 cut(s) 149, 428
BtgZI GCGATG 2 cut(s) 330, 358
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 379, 633
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 212, 368
ClaI ATCGAT 2 cut(s) 149, 428
Csp6I GTAC 2 cut(s) 340, 547
CviAII CATG 1 cut(s) 589
CviQI GTAC 2 cut(s) 340, 547
DdeI CTNAG 3 cut(s) 280, 540, 644
DpnI GATC 7 cut(s) 78, 198, 263, 290, 427, 431, 500
DpnII GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
Eam1104I CTCTTC 3 cut(s) 315, 335, 392
EarI CTCTTC 3 cut(s) 315, 335, 392
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 212, 368
Eco47III AGCGCT 1 cut(s) 636
Eco53kI GAGCTC 1 cut(s) 457
Eco88I CYCGRG 1 cut(s) 626
EcoICRI GAGCTC 1 cut(s) 457
EcoO109I RGGNCCY 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 592
FaiI YATR 3 cut(s) 294, 392, 590
FaqI GGGAC 1 cut(s) 354
FatI CATG 1 cut(s) 588
FauI CCCGC 1 cut(s) 55
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 1 cut(s) 418
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 1 cut(s) 418
FspBI CTAG 1 cut(s) 258
GlaI GCGC 1 cut(s) 636
GluI GCNGC 1 cut(s) 418
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 1 cut(s) 381
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 637
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 95, 612
HinfI GANTC 3 cut(s) 300, 517, 594
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 11
Hpy166II GTNNAC 4 cut(s) 19, 469, 511, 568
Hpy188I TCNGA 1 cut(s) 543
Hpy188III TCNNGA 4 cut(s) 49, 200, 235, 598
Hpy8I GTNNAC 4 cut(s) 19, 469, 511, 568
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 2 cut(s) 232, 565
HpyCH4V TGCA 3 cut(s) 410, 417, 495
HpyF3I CTNAG 3 cut(s) 280, 540, 644
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 1 cut(s) 141
MaeI CTAG 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 65, 559
MalI GATC 7 cut(s) 78, 198, 263, 290, 427, 431, 500
MboI GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
MboII GAAGA 6 cut(s) 105, 175, 298, 322, 332, 379
MflI RGATCY 2 cut(s) 261, 288
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 1 cut(s) 405
MlyI GAGTC 2 cut(s) 294, 526
MnlI CCTC 3 cut(s) 269, 456, 635
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 1 cut(s) 306
MspI CCGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 410
NdeII GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 2 cut(s) 263, 370
NmuCI GTSAC 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 626
PctI GAATGC 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 1 cut(s) 419
PleI GAGTC 2 cut(s) 294, 525
PpsI GAGTC 2 cut(s) 294, 525
PpuMI RGGWCCY 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 368
PspN4I GGNNCC 2 cut(s) 263, 370
PspPI GGNCC 2 cut(s) 212, 368
PspPPI RGGWCCY 1 cut(s) 368
PspXI VCTCGAGB 1 cut(s) 626
PstI CTGCAG 1 cut(s) 419
PsuI RGATCY 2 cut(s) 261, 288
RsaI GTAC 2 cut(s) 341, 548
RsaNI GTAC 2 cut(s) 340, 547
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 566
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 7 cut(s) 76, 196, 261, 288, 425, 429, 498
Sau96I GGNCC 2 cut(s) 212, 368
SchI GAGTC 2 cut(s) 294, 526
SduI GDGCHC 1 cut(s) 459
SfaNI GCATC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 415
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 2 cut(s) 212, 368
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 3 cut(s) 447, 598, 626
SmoI CTYRAG 3 cut(s) 447, 598, 626
Sse9I AATT 1 cut(s) 405
SsiI CCGC 2 cut(s) 62, 363
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 459
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 232, 565
TaqI TCGA 4 cut(s) 149, 428, 567, 627
TasI AATT 1 cut(s) 405
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 333, 379, 577
TspGWI ACGGA 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 212, 368
XhoI CTCGAG 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.