Rroxscaffold_5G00387300

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003141
Physical Location & Seq
Reverse (-)
65989274 .. 65990751
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387300.1

Sequence Viewer

Length: 687 bp
ATGAAGGCTTCATTTCAGGTTCAGCTTGGAGGACAAGTTCTTGGTTTCCCAATCAATTCAGCAGCAGCAGCAGCATCATGCACATTTGAGAGGTCCCCTATGAATGCAAAGAGACTCCTGCTACCTGCTCAGCATCATGTTGCTATTCAGTATGATCATGTTCCATCATCTGATCATCGTCGTGATCAAATTGGGTCTTCAAAACGGACACAAATGAAACTGAGGCCTAAAATCAGTGAGACAGTGAAGGGAAAGTTGAGACTTGGGGCTAAGATTCTTAGAGCAGGTGGAACAATGGAGAAGGTGTTCAAGCATTTGTTTAGTAATAATGATAAAGCAGCAGGAGAGAAGCTTTTGAAGACTTCTCACTGTTATCTATCAACCACAGCAGGTCCTATTGCAGGCCTCCTTTTCATCTCCACGCACAAGGTTGCCTTCTGCAGTGACAAGCCAATCAAACTGTCATCATCGCCAAATCCAAATGGACAACTCATCAGAGTCCACTACAAGGTTGTGATCCCACTCAATAAGATAAACAGAGTAAACCAGAGTGAGGATGTGAAGAAGCCATCACAGAAGTACATAGAAATAGTTACTGTGGATAATTATGACTTCTGGTTTATGGGTTTCTTCAATTACCAGAAAGCTCACAAATATCTCCAACAGGCATTAATTTCTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

228

Amino Acids

25.47

Weight (kDa)

10.01

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 103 - 224 4.1e-16 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 275
Acc36I ACCTGC 3 cut(s) 133, 275, 380
AclWI GGATC 1 cut(s) 511
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 3 cut(s) 401, 508, 553
AgsI TTSAA 4 cut(s) 201, 310, 358, 634
AloI GAACNNNNNNTCC 4 cut(s) 21, 53, 290, 322
AluBI AGCT 3 cut(s) 25, 352, 647
AluI AGCT 3 cut(s) 25, 352, 647
Alw26I GTCTC 3 cut(s) 106, 233, 253
AlwI GGATC 1 cut(s) 511
AoxI GGCC 2 cut(s) 224, 403
ApeKI GCWGC 5 cut(s) 62, 65, 68, 71, 338
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 93, 392
AvaII GGWCC 2 cut(s) 93, 392
BbsI GAAGAC 2 cut(s) 189, 365
BbvI GCAGC 5 cut(s) 74, 77, 80, 83, 350
BccI CCATC 2 cut(s) 172, 577
BclI TGATCA 3 cut(s) 154, 172, 184
BcoDI GTCTC 3 cut(s) 106, 233, 253
BfmI CTRYAG 1 cut(s) 439
BfuAI ACCTGC 3 cut(s) 133, 275, 380
BisI GCNGC 5 cut(s) 63, 66, 69, 72, 339
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 5 cut(s) 64, 67, 70, 73, 340
Bme18I GGWCC 2 cut(s) 93, 392
BmgT120I GGNCC 2 cut(s) 93, 392
BmiI GGNNCC 1 cut(s) 95
BmsI GCATC 2 cut(s) 83, 142
BpiI GAAGAC 2 cut(s) 189, 365
Bpu1102I GCTNAGC 1 cut(s) 129
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 290, 320
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 508, 553
BseGI GGATG 1 cut(s) 562
BseLI CCNNNNNNNGG 3 cut(s) 401, 508, 553
BseMII CTCAG 2 cut(s) 143, 212
BseXI GCAGC 5 cut(s) 74, 77, 80, 83, 350
BshFI GGCC 2 cut(s) 226, 405
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 3 cut(s) 401, 508, 553
BsmAI GTCTC 3 cut(s) 106, 233, 253
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 2 cut(s) 226, 405
Bsp143I GATC 4 cut(s) 154, 172, 184, 516
Bsp1720I GCTNAGC 1 cut(s) 129
BspANI GGCC 2 cut(s) 226, 405
BspCNI CTCAG 2 cut(s) 142, 213
BspLI GGNNCC 1 cut(s) 95
BspMAI CTGCAG 1 cut(s) 443
BspMI ACCTGC 3 cut(s) 133, 275, 380
BspPI GGATC 1 cut(s) 511
BssMI GATC 4 cut(s) 154, 172, 184, 516
Bst4CI ACNGT 4 cut(s) 244, 371, 462, 598
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 6 cut(s) 129, 221, 270, 278, 678, 684
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 562
BstKTI GATC 4 cut(s) 157, 175, 187, 519
BstMAI GTCTC 3 cut(s) 106, 233, 253
BstMBI GATC 4 cut(s) 154, 172, 184, 516
BstMWI GCNNNNNNNGC 2 cut(s) 68, 71
BstSFI CTRYAG 1 cut(s) 439
BstV1I GCAGC 5 cut(s) 74, 77, 80, 83, 350
BstV2I GAAGAC 2 cut(s) 189, 365
BsuRI GGCC 2 cut(s) 226, 405
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 562
BtsI GCAGTG 1 cut(s) 448
BtsIMutI CAGTG 4 cut(s) 241, 249, 367, 448
BveI ACCTGC 3 cut(s) 133, 275, 380
Cac8I GCNNGC 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 93, 392
Csp6I GTAC 1 cut(s) 580
CviAII CATG 3 cut(s) 78, 137, 158
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 6 cut(s) 129, 221, 270, 278, 678, 684
DpnI GATC 4 cut(s) 156, 174, 186, 518
DpnII GATC 4 cut(s) 154, 172, 184, 516
Eco147I AGGCCT 2 cut(s) 226, 405
Eco47I GGWCC 2 cut(s) 93, 392
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoO109I RGGNCCY 2 cut(s) 93, 392
FaeI CATG 3 cut(s) 81, 140, 161
FaiI YATR 8 cut(s) 79, 101, 138, 153, 159, 584, 609, 623
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 79
FatI CATG 3 cut(s) 77, 136, 157
FbaI TGATCA 3 cut(s) 154, 172, 184
Fnu4HI GCNGC 5 cut(s) 63, 66, 69, 72, 339
FokI GGATG 1 cut(s) 569
Fsp4HI GCNGC 5 cut(s) 63, 66, 69, 72, 339
GluI GCNGC 5 cut(s) 63, 66, 69, 72, 339
HaeIII GGCC 2 cut(s) 226, 405
Hin1II CATG 3 cut(s) 81, 140, 161
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 3 cut(s) 114, 274, 498
Hpy166II GTNNAC 2 cut(s) 502, 544
Hpy188I TCNGA 2 cut(s) 172, 497
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 2 cut(s) 502, 544
Hpy99I CGWCG 1 cut(s) 183
HpyAV CCTTC 3 cut(s) 241, 295, 445
HpyCH4III ACNGT 4 cut(s) 244, 371, 462, 598
HpyCH4V TGCA 4 cut(s) 81, 107, 401, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 71
HpyF3I CTNAG 6 cut(s) 129, 221, 270, 278, 678, 684
Hsp92II CATG 3 cut(s) 81, 140, 161
Ksp22I TGATCA 3 cut(s) 154, 172, 184
Kzo9I GATC 4 cut(s) 154, 172, 184, 516
Lsp1109I GCAGC 5 cut(s) 74, 77, 80, 83, 350
LweI GCATC 2 cut(s) 83, 142
MaeIII GTNAC 2 cut(s) 443, 592
MalI GATC 4 cut(s) 156, 174, 186, 518
MboI GATC 4 cut(s) 154, 172, 184, 516
MboII GAAGA 4 cut(s) 189, 370, 574, 622
MluCI AATT 5 cut(s) 55, 189, 604, 634, 672
MlyI GAGTC 2 cut(s) 108, 507
MmeI TCCRAC 1 cut(s) 685
MnlI CCTC 5 cut(s) 23, 84, 216, 416, 547
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 1 cut(s) 671
MslI CAYNNNNRTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 68, 71
NdeII GATC 4 cut(s) 154, 172, 184, 516
NlaIII CATG 3 cut(s) 81, 140, 161
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 275
PceI AGGCCT 2 cut(s) 226, 405
PctI GAATGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 5 cut(s) 64, 67, 70, 73, 340
PleI GAGTC 2 cut(s) 108, 506
PpsI GAGTC 2 cut(s) 108, 506
PpuMI RGGWCCY 2 cut(s) 93, 392
PshBI ATTAAT 1 cut(s) 671
Psp5II RGGWCCY 2 cut(s) 93, 392
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 2 cut(s) 93, 392
PspPPI RGGWCCY 2 cut(s) 93, 392
PstI CTGCAG 1 cut(s) 443
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 1 cut(s) 671
SatI GCNGC 5 cut(s) 63, 66, 69, 72, 339
Sau3AI GATC 4 cut(s) 154, 172, 184, 516
Sau96I GGNCC 2 cut(s) 93, 392
SchI GAGTC 2 cut(s) 108, 507
SfaNI GCATC 2 cut(s) 83, 142
SfcI CTRYAG 1 cut(s) 439
SinI GGWCC 2 cut(s) 93, 392
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 5 cut(s) 55, 189, 604, 634, 672
SseBI AGGCCT 2 cut(s) 226, 405
StuI AGGCCT 2 cut(s) 226, 405
TaaI ACNGT 4 cut(s) 244, 371, 462, 598
TasI AATT 5 cut(s) 55, 189, 604, 634, 672
TatI WGTACW 1 cut(s) 579
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 1 cut(s) 671
Tru9I TTAA 1 cut(s) 671
TscAI CASTG 4 cut(s) 241, 249, 374, 448
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 5 cut(s) 62, 65, 68, 71, 338
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 4 cut(s) 17, 116, 230, 403
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 4 cut(s) 241, 249, 374, 448
VpaK11BI GGWCC 2 cut(s) 93, 392
VspI ATTAAT 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.