RLG00000005616

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003141
Physical Location & Seq
Forward (+)
305992 .. 307192
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005616

Sequence Viewer

Length: 483 bp
ATGTGTGCAGTGAAACTGAGGCCTAAAATCAGTGAGACAGTGAAGGGAAAGTTGAGACTTGGGGCTAAGATTCTTAGAGCAGGTGGAACAATGGAGAAGGTGTTCAAGCATTTGTTTAGTAATAATGATAAAGCAGCAGGAGAGAAGCTTTTGAAGACTTCTCACTGTTATCTATCAACCACAGCAGGTCCTATTGCAGGCCTCCTTTTCATCTCCACACACAAGATTGCCTTCTGCAGTGACAAGCCAATCAAACTGTCATCATCGCCAAATCCAAATGGACAACTCATCAGAGTCCACTACAAGGTTGTGATCCCACTCAATAAGATAAACAGAGTAAACCAGAGTGAGGATGTGAAGAAGCCATCACAGAAGTACATAGAAATAGTTACTGTGGATAATTATGACTTCTGGTTTATGGGTTTCTTCAATTACCAGAAAGCTCACAAATATCTCCAACGGGCATTAATTTCTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

161

Amino Acids

18.04

Weight (kDa)

9.95

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 35 - 156 1.8e-16 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 71
Acc36I ACCTGC 2 cut(s) 71, 176
AclWI GGATC 1 cut(s) 307
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 3 cut(s) 197, 304, 349
AgsI TTSAA 3 cut(s) 106, 154, 430
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
AluBI AGCT 2 cut(s) 148, 443
AluI AGCT 2 cut(s) 148, 443
Alw26I GTCTC 2 cut(s) 29, 49
AlwI GGATC 1 cut(s) 307
AoxI GGCC 2 cut(s) 20, 199
ApeKI GCWGC 1 cut(s) 134
AseI ATTAAT 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 188
AvaII GGWCC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 29, 49
BfmI CTRYAG 1 cut(s) 235
BfuAI ACCTGC 2 cut(s) 71, 176
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 304, 349
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 3 cut(s) 197, 304, 349
BseMII CTCAG 1 cut(s) 8
BseXI GCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 27
BshFI GGCC 2 cut(s) 22, 201
BslI CCNNNNNNNGG 3 cut(s) 197, 304, 349
BsmAI GTCTC 2 cut(s) 29, 49
BsnI GGCC 2 cut(s) 22, 201
Bsp143I GATC 1 cut(s) 312
BspANI GGCC 2 cut(s) 22, 201
BspCNI CTCAG 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 239
BspMI ACCTGC 2 cut(s) 71, 176
BspPI GGATC 1 cut(s) 307
BssMI GATC 1 cut(s) 312
Bst4CI ACNGT 4 cut(s) 40, 167, 258, 394
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 5 cut(s) 17, 66, 74, 474, 480
BstENI CCTNNNNNAGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 358
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 29, 49
BstMBI GATC 1 cut(s) 312
BstSFI CTRYAG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 22, 201
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 2 cut(s) 15, 244
BtsIMutI CAGTG 5 cut(s) 15, 37, 45, 163, 244
BveI ACCTGC 2 cut(s) 71, 176
Cac8I GCNNGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 376
CviJI RGCY 8 cut(s) 22, 65, 148, 201, 247, 364, 443, 479
CviKI_1 RGCY 8 cut(s) 22, 65, 148, 201, 247, 364, 443, 479
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 5 cut(s) 17, 66, 74, 474, 480
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eco147I AGGCCT 2 cut(s) 22, 201
Eco47I GGWCC 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 188
FaiI YATR 3 cut(s) 380, 405, 419
FalI AAGNNNNNCTT 2 cut(s) 215, 247
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 2 cut(s) 22, 201
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 2 cut(s) 70, 294
Hpy166II GTNNAC 2 cut(s) 298, 340
Hpy188I TCNGA 1 cut(s) 293
Hpy8I GTNNAC 2 cut(s) 298, 340
HpyAV CCTTC 3 cut(s) 37, 91, 241
HpyCH4III ACNGT 4 cut(s) 40, 167, 258, 394
HpyCH4V TGCA 3 cut(s) 8, 197, 237
HpyF3I CTNAG 5 cut(s) 17, 66, 74, 474, 480
Kzo9I GATC 1 cut(s) 312
LpnPI CCDG 8 cut(s) 66, 123, 171, 183, 356, 397, 449, 461
Lsp1109I GCAGC 1 cut(s) 146
MaeIII GTNAC 2 cut(s) 239, 388
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 3 cut(s) 166, 370, 418
MluCI AATT 3 cut(s) 400, 430, 468
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 3 cut(s) 12, 212, 343
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 1 cut(s) 467
NdeII GATC 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 239
PaqCI CACCTGC 1 cut(s) 71
PceI AGGCCT 2 cut(s) 22, 201
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 188
PspPI GGNCC 1 cut(s) 188
PspPPI RGGWCCY 1 cut(s) 188
PstI CTGCAG 1 cut(s) 239
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 303
SetI ASST 6 cut(s) 85, 102, 150, 190, 309, 445
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 188
Sse9I AATT 3 cut(s) 400, 430, 468
SseBI AGGCCT 2 cut(s) 22, 201
StuI AGGCCT 2 cut(s) 22, 201
TaaI ACNGT 4 cut(s) 40, 167, 258, 394
TasI AATT 3 cut(s) 400, 430, 468
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 5 cut(s) 15, 37, 45, 170, 244
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 199
TspRI CASTG 5 cut(s) 15, 37, 45, 170, 244
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.