AT5G23350

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003141
Physical Location & Seq
Reverse (-)
7858545 .. 7859201
657 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23350.1

Sequence Viewer

Length: 657 bp
ATGACACTGAGCAGAGTTCACCAACAAGTTATTGCATTTCCTGCAGCCAAGACTGATACGATGAGTTACTTGCCTGACCCAGCTTCCATCAACAACCACCAAATCCCAACTTCTTCAAAGGTTTCATATTTGACAGGCAAAGGAAAATCGATGCTGCGAAAGAAGAAAACCGATAGCTTCACAAACGGAGCTAGAGATCAGGACAAACTAGGACCAAAGCTAACTGAAACAGTCAAGAGAAAGCTATCCTTGGGAGCTAAGATCCTTCAAATGGGAGGCTTAGAGAAGATTTATAAGCGACTCTTCAAGGTCTGCGATCAAGAGAAACTCTTCAAGGCGTACCAATGCTATCTATCCACAACAGCAGGTCCCATCGCAGGCCTCCTCTTCATCTCATCAAAGAAGATTGCATTTTGCAGTGAAAGATCGATCAAGGTTGCTTCTCCTCAAGGAGTTCTCAGTAGGGTTCACTACAAAGTCTCAATCCCATTGTGCAAGATCAATGGAGTGAACCAGAGTCAGAACACAAAGAAGCCATCTCAAAAGTACCTTGAAATAGTCACAATCGATAACTTCGACTTCTGGTTCATGGGATTCGTGAGCTACCAGAAAGCTTTCAACTGTCTCGAGAAAGCGCTAAACAATGATGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

218

Amino Acids

24.47

Weight (kDa)

9.76

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 99 - 215 2.1e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 356
AclWI GGATC 1 cut(s) 256
AfaI GTAC 2 cut(s) 341, 548
AfeI AGCGCT 1 cut(s) 636
AfiI CCNNNNNNNGG 2 cut(s) 271, 377
AgsI TTSAA 6 cut(s) 117, 269, 307, 334, 554, 619
AluBI AGCT 8 cut(s) 83, 177, 191, 220, 244, 257, 603, 614
AluI AGCT 8 cut(s) 83, 177, 191, 220, 244, 257, 603, 614
Alw26I GTCTC 2 cut(s) 484, 629
AlwI GGATC 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 626
Aor51HI AGCGCT 1 cut(s) 636
AoxI GGCC 1 cut(s) 379
ApeKI GCWGC 2 cut(s) 44, 154
Asp700I GAANNNNTTC 2 cut(s) 329, 614
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 212, 368
AsuHPI GGTGA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 2 cut(s) 212, 368
BbvI GCAGC 2 cut(s) 56, 141
BccI CCATC 3 cut(s) 95, 380, 544
BcoDI GTCTC 2 cut(s) 484, 629
BfaI CTAG 2 cut(s) 192, 209
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 356
BisI GCNGC 2 cut(s) 45, 155
BlsI GCNGC 2 cut(s) 46, 156
Bme18I GGWCC 2 cut(s) 212, 368
BmeT110I CYCGRG 1 cut(s) 626
BmgT120I GGNCC 2 cut(s) 212, 368
BmiI GGNNCC 1 cut(s) 370
BmsI GCATC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 432
Bsa29I ATCGAT 3 cut(s) 149, 428, 567
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 377
BseCI ATCGAT 3 cut(s) 149, 428, 567
BseDI CCNNGG 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 271, 377
BseMII CTCAG 1 cut(s) 472
BseRI GAGGAG 2 cut(s) 374, 435
BseXI GCAGC 2 cut(s) 56, 141
BseYI CCCAGC 1 cut(s) 79
BshFI GGCC 1 cut(s) 381
BshVI ATCGAT 3 cut(s) 149, 428, 567
BsiHKCI CYCGRG 1 cut(s) 626
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 271, 377
BsmAI GTCTC 2 cut(s) 484, 629
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 1 cut(s) 381
BsoBI CYCGRG 1 cut(s) 626
Bsp143I GATC 6 cut(s) 196, 261, 316, 425, 429, 498
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 1 cut(s) 471
BspDI ATCGAT 3 cut(s) 149, 428, 567
BspLI GGNNCC 1 cut(s) 370
BspMAI CTGCAG 1 cut(s) 46
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 6 cut(s) 196, 261, 316, 425, 429, 498
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 232, 623
Bst6I CTCTTC 3 cut(s) 308, 335, 392
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 4 cut(s) 8, 258, 280, 458
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 6 cut(s) 199, 264, 319, 428, 432, 501
BstMAI GTCTC 2 cut(s) 484, 629
BstMBI GATC 6 cut(s) 196, 261, 316, 425, 429, 498
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 56, 141
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
Bsu15I ATCGAT 3 cut(s) 149, 428, 567
BsuRI GGCC 1 cut(s) 381
BsuTUI ATCGAT 3 cut(s) 149, 428, 567
BtgZI GCGATG 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 2 cut(s) 5, 424
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 1 cut(s) 379
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 212, 368
ClaI ATCGAT 3 cut(s) 149, 428, 567
Csp6I GTAC 2 cut(s) 340, 547
CviAII CATG 1 cut(s) 589
CviQI GTAC 2 cut(s) 340, 547
DdeI CTNAG 4 cut(s) 8, 258, 280, 458
DpnI GATC 6 cut(s) 198, 263, 318, 427, 431, 500
DpnII GATC 6 cut(s) 196, 261, 316, 425, 429, 498
Eam1104I CTCTTC 3 cut(s) 308, 335, 392
EarI CTCTTC 3 cut(s) 308, 335, 392
Eco130I CCWWGG 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 212, 368
Eco47III AGCGCT 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 592
FaiI YATR 3 cut(s) 127, 294, 590
FalI AAGNNNNNCTT 4 cut(s) 233, 265, 287, 319
FaqI GGGAC 1 cut(s) 354
FatI CATG 1 cut(s) 588
Fnu4HI GCNGC 2 cut(s) 45, 155
Fsp4HI GCNGC 2 cut(s) 45, 155
FspBI CTAG 2 cut(s) 192, 209
GlaI GCGC 1 cut(s) 636
GluI GCNGC 2 cut(s) 45, 155
GsaI CCCAGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 1 cut(s) 381
HhaI GCGC 1 cut(s) 637
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 3 cut(s) 300, 517, 594
HphI GGTGA 1 cut(s) 11
Hpy166II GTNNAC 3 cut(s) 19, 469, 511
Hpy188I TCNGA 1 cut(s) 522
Hpy188III TCNNGA 6 cut(s) 200, 235, 320, 598, 626, 628
Hpy8I GTNNAC 3 cut(s) 19, 469, 511
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 2 cut(s) 232, 623
HpyCH4V TGCA 5 cut(s) 35, 44, 410, 417, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 4 cut(s) 8, 258, 280, 458
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 6 cut(s) 196, 261, 316, 425, 429, 498
LmnI GCTCC 2 cut(s) 188, 254
Lsp1109I GCAGC 2 cut(s) 56, 141
LweI GCATC 1 cut(s) 141
MaeI CTAG 2 cut(s) 192, 209
MaeIII GTNAC 2 cut(s) 65, 559
MalI GATC 6 cut(s) 198, 263, 318, 427, 431, 500
MboI GATC 6 cut(s) 196, 261, 316, 425, 429, 498
MboII GAAGA 7 cut(s) 105, 175, 295, 298, 322, 379, 415
MflI RGATCY 1 cut(s) 261
MlyI GAGTC 2 cut(s) 294, 526
MnlI CCTC 4 cut(s) 269, 392, 395, 456
MroXI GAANNNNTTC 2 cut(s) 329, 614
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 6 cut(s) 196, 261, 316, 425, 429, 498
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 626
PceI AGGCCT 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PdmI GAANNNNTTC 2 cut(s) 329, 614
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 2 cut(s) 46, 156
PleI GAGTC 2 cut(s) 294, 525
PpsI GAGTC 2 cut(s) 294, 525
PpuMI RGGWCCY 1 cut(s) 368
PsiI TTATAA 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 212, 368
PspPPI RGGWCCY 1 cut(s) 368
PstI CTGCAG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 2 cut(s) 341, 548
RsaNI GTAC 2 cut(s) 340, 547
SatI GCNGC 2 cut(s) 45, 155
Sau3AI GATC 6 cut(s) 196, 261, 316, 425, 429, 498
Sau96I GGNCC 2 cut(s) 212, 368
SchI GAGTC 2 cut(s) 294, 526
SfaNI GCATC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 2 cut(s) 212, 368
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 2 cut(s) 447, 626
SmoI CTYRAG 2 cut(s) 447, 626
SseBI AGGCCT 1 cut(s) 381
SspMI CTAG 2 cut(s) 192, 209
StuI AGGCCT 1 cut(s) 381
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 232, 623
TaqI TCGA 5 cut(s) 149, 428, 567, 576, 627
TfiI GAWTC 1 cut(s) 594
TscAI CASTG 2 cut(s) 12, 424
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 44, 154
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 114, 379, 577
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 2 cut(s) 12, 424
VpaK11BI GGWCC 2 cut(s) 212, 368
XhoI CTCGAG 1 cut(s) 626
XmnI GAANNNNTTC 2 cut(s) 329, 614
XspI CTAG 2 cut(s) 192, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.