MD00G1126700.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003141
Physical Location & Seq
Reverse (-)
26919135 .. 26920092
958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1126700.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAAGGCCTCACTTCAAGGACAAGTTATAGGAATTCCAATCAACTCCGCAGCCGCGTACTCGTTTAAGAGTGCAGATCATCGTGCTGCTATCCAATATCATCATAATATTCCAGCAACGGCTCCTAGTGATGCTACTGAACCTTCAAAGCTCATCAGACAAAGCAGACTGCATTCAGTGCTTAACAGGATGAACAAGCTTACCAACAAGGCTGAAAGTTTTGCCCATGGAGTCAAAGAACATGTGAGACTAGGGCCCAAGATCACAGAGACAGTGAAGGGGAAGCTGAGGCTTGGGGCTAAGATTGTAAAAGCAGGAGGGACGTTAGAGGTGTTCAAGCACGCATTCAGCGTTAACGCAGGAGAGAAGCTGCTCAAGGCATCCCAGTGTTATCTGTCGACCACAGCGGGTCCTATGGCAGGCCTCCTCTTCATGTCGACGGACAAGATTGCGTTTTGCAGCGAGAGATCGATCAAACTTCCATCGTCAGATTCAGACGGACAACAGTTGGTCAGCAGAGTCCACTACAAGGTGGTGATCCCAGTGAAGAAGATCAAGGCGGTCAACCAGAGCGAGAACAGGAAGAAGCCGTCGCAAAAGTACGTAGAAATAGTTACTGCGGATGATTTTGACTTCTGGTTTATGGGGATCTTGAATTACCAGAAAACTTTGAAATATCTCCAGCAGGCTATTAATATTCCATCTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

238

Amino Acids

26.12

Weight (kDa)

9.9

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 111 - 231 4.2e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 398, 437
AccII CGCG 1 cut(s) 56
AciI CCGC 5 cut(s) 48, 54, 407, 560, 620
AclWI GGATC 2 cut(s) 532, 656
AcsI RAATTY 1 cut(s) 33
AfaI GTAC 2 cut(s) 59, 602
AfiI CCNNNNNNNGG 2 cut(s) 419, 529
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 5 cut(s) 17, 147, 337, 655, 673
AluBI AGCT 5 cut(s) 151, 199, 286, 370, 710
AluI AGCT 5 cut(s) 151, 199, 286, 370, 710
Alw26I GTCTC 2 cut(s) 241, 263
AlwI GGATC 2 cut(s) 532, 656
AoxI GGCC 3 cut(s) 6, 254, 421
ApaI GGGCCC 1 cut(s) 258
ApeKI GCWGC 4 cut(s) 50, 86, 370, 459
ApoI RAATTY 1 cut(s) 33
AseI ATTAAT 1 cut(s) 693
AspS9I GGNCC 3 cut(s) 254, 255, 410
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 4 cut(s) 62, 73, 357, 471
BccI CCATC 2 cut(s) 490, 709
BceAI ACGGC 2 cut(s) 135, 574
BcoDI GTCTC 2 cut(s) 241, 263
BfaI CTAG 2 cut(s) 126, 251
BisI GCNGC 5 cut(s) 51, 54, 87, 371, 460
BlsI GCNGC 5 cut(s) 52, 55, 88, 372, 461
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 3 cut(s) 254, 255, 410
BmiI GGNNCC 3 cut(s) 123, 256, 411
BmrI ACTGGG 2 cut(s) 379, 536
BmsI GCATC 2 cut(s) 121, 389
BmuI ACTGGG 2 cut(s) 379, 536
BpmI CTGGAG 1 cut(s) 665
Bpu10I CCTNAGC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 359, 690
Bsa29I ATCGAT 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 604
BsaJI CCNNGG 1 cut(s) 226
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 529
Bse1I ACTGG 2 cut(s) 385, 542
BseCI ATCGAT 1 cut(s) 470
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 3 cut(s) 195, 380, 628
BseLI CCNNNNNNNGG 2 cut(s) 419, 529
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 2 cut(s) 385, 542
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 4 cut(s) 62, 73, 357, 471
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 8, 256, 423
BshVI ATCGAT 1 cut(s) 470
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 2 cut(s) 419, 529
BsmAI GTCTC 2 cut(s) 241, 263
BsmFI GGGAC 1 cut(s) 334
BsmI GAATGC 2 cut(s) 172, 344
BsnI GGCC 3 cut(s) 8, 256, 423
Bsp120I GGGCCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 5 cut(s) 48, 54, 407, 560, 620
BspANI GGCC 3 cut(s) 8, 256, 423
BspCNI CTCAG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 470
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 3 cut(s) 123, 256, 411
BspPI GGATC 2 cut(s) 532, 656
BsrI ACTGG 2 cut(s) 385, 542
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
BssT1I CCWWGG 1 cut(s) 226
Bst4CI ACNGT 2 cut(s) 274, 507
Bst6I CTCTTC 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 178
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 342, 421, 687
BstDEI CTNAG 2 cut(s) 287, 300
BstDSI CCRYGG 1 cut(s) 226
BstENI CCTNNNNNAGG 1 cut(s) 417
BstF5I GGATG 3 cut(s) 195, 380, 628
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 7 cut(s) 79, 264, 470, 474, 540, 555, 651
BstMAI GTCTC 2 cut(s) 241, 263
BstMBI GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNSI RCATGY 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 258
BstSNI TACGTA 1 cut(s) 604
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 4 cut(s) 62, 73, 357, 471
BstX2I RGATCY 1 cut(s) 648
BstYI RGATCY 1 cut(s) 648
Bsu15I ATCGAT 1 cut(s) 470
BsuRI GGCC 3 cut(s) 8, 256, 423
BsuTUI ATCGAT 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 3 cut(s) 195, 380, 628
BtsIMutI CAGTG 4 cut(s) 183, 279, 392, 549
Cac8I GCNNGC 3 cut(s) 342, 421, 687
Cfr13I GGNCC 3 cut(s) 254, 255, 410
ClaI ATCGAT 1 cut(s) 470
Csp6I GTAC 2 cut(s) 58, 601
CviAII CATG 3 cut(s) 227, 242, 433
CviQI GTAC 2 cut(s) 58, 601
DdeI CTNAG 2 cut(s) 287, 300
DpnI GATC 7 cut(s) 78, 263, 469, 473, 539, 554, 650
DpnII GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Eco105I TACGTA 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 226
Eco147I AGGCCT 2 cut(s) 8, 423
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 417
EcoO109I RGGNCCY 2 cut(s) 254, 410
EcoRI GAATTC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 3 cut(s) 230, 245, 436
FaiI YATR 7 cut(s) 29, 105, 228, 243, 416, 434, 644
FaqI GGGAC 1 cut(s) 334
FatI CATG 3 cut(s) 226, 241, 432
FauI CCCGC 1 cut(s) 400
FblI GTMKAC 2 cut(s) 398, 437
Fnu4HI GCNGC 5 cut(s) 51, 54, 87, 371, 460
FokI GGATG 3 cut(s) 202, 367, 635
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 5 cut(s) 51, 54, 87, 371, 460
FspBI CTAG 2 cut(s) 126, 251
GluI GCNGC 5 cut(s) 51, 54, 87, 371, 460
GsuI CTGGAG 1 cut(s) 665
HaeIII GGCC 3 cut(s) 8, 256, 423
Hin1II CATG 3 cut(s) 230, 245, 436
HincII GTYRAC 4 cut(s) 355, 399, 438, 565
HindII GTYRAC 4 cut(s) 355, 399, 438, 565
HindIII AAGCTT 2 cut(s) 197, 708
HinfI GANTC 3 cut(s) 231, 491, 519
HpaI GTTAAC 1 cut(s) 355
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 5 cut(s) 355, 399, 438, 523, 565
Hpy188I TCNGA 3 cut(s) 158, 490, 496
Hpy188III TCNNGA 1 cut(s) 652
Hpy8I GTNNAC 5 cut(s) 355, 399, 438, 523, 565
Hpy99I CGWCG 2 cut(s) 442, 595
HpyAV CCTTC 2 cut(s) 153, 271
HpyCH4III ACNGT 2 cut(s) 274, 507
HpyCH4IV ACGT 2 cut(s) 323, 603
HpyCH4V TGCA 3 cut(s) 74, 172, 459
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 2 cut(s) 287, 300
HpySE526I ACGT 2 cut(s) 323, 603
Hsp92II CATG 3 cut(s) 230, 245, 436
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 4 cut(s) 62, 73, 357, 471
LweI GCATC 2 cut(s) 121, 389
MaeI CTAG 2 cut(s) 126, 251
MaeII ACGT 2 cut(s) 323, 603
MaeIII GTNAC 1 cut(s) 613
MalI GATC 7 cut(s) 78, 263, 469, 473, 539, 554, 650
MboI GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
MboII GAAGA 4 cut(s) 421, 559, 562, 595
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 2 cut(s) 33, 655
MlyI GAGTC 2 cut(s) 240, 528
MnlI CCTC 6 cut(s) 19, 282, 311, 322, 434, 437
MseI TTAA 5 cut(s) 66, 183, 354, 693, 712
MslI CAYNNNNRTG 1 cut(s) 385
MspA1I CMGCKG 1 cut(s) 407
Mva1269I GAATGC 2 cut(s) 172, 344
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 178
NcoI CCATGG 1 cut(s) 226
NdeII GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
NlaIII CATG 3 cut(s) 230, 245, 436
NlaIV GGNNCC 3 cut(s) 123, 256, 411
NspI RCATGY 1 cut(s) 245
PceI AGGCCT 2 cut(s) 8, 423
PciI ACATGT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PctI GAATGC 2 cut(s) 172, 344
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 5 cut(s) 52, 55, 88, 372, 461
PleI GAGTC 2 cut(s) 239, 527
PpsI GAGTC 2 cut(s) 239, 527
Ppu21I YACGTR 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 410
PscI ACATGT 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 410
PspN4I GGNNCC 3 cut(s) 123, 256, 411
PspOMI GGGCCC 1 cut(s) 254
PspPI GGNCC 3 cut(s) 254, 255, 410
PspPPI RGGWCCY 1 cut(s) 410
PsuI RGATCY 1 cut(s) 648
RsaI GTAC 2 cut(s) 59, 602
RsaNI GTAC 2 cut(s) 58, 601
RseI CAYNNNNRTG 1 cut(s) 385
SalI GTCGAC 2 cut(s) 397, 436
SaqAI TTAA 5 cut(s) 66, 183, 354, 693, 712
SatI GCNGC 5 cut(s) 51, 54, 87, 371, 460
Sau3AI GATC 7 cut(s) 76, 261, 467, 471, 537, 552, 648
Sau96I GGNCC 3 cut(s) 254, 255, 410
SchI GAGTC 2 cut(s) 240, 528
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 2 cut(s) 121, 389
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 385
SmlI CTYRAG 2 cut(s) 374, 705
SmoI CTYRAG 2 cut(s) 374, 705
SnaBI TACGTA 1 cut(s) 604
Sse9I AATT 2 cut(s) 33, 655
SseBI AGGCCT 2 cut(s) 8, 423
SsiI CCGC 5 cut(s) 48, 54, 407, 560, 620
SspI AATATT 2 cut(s) 109, 697
SspMI CTAG 2 cut(s) 126, 251
StuI AGGCCT 2 cut(s) 8, 423
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 2 cut(s) 274, 507
TaiI ACGT 2 cut(s) 326, 606
TaqI TCGA 3 cut(s) 398, 437, 470
TasI AATT 2 cut(s) 33, 655
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 5 cut(s) 66, 183, 354, 693, 712
Tru9I TTAA 5 cut(s) 66, 183, 354, 693, 712
TscAI CASTG 4 cut(s) 183, 279, 392, 549
TseI GCWGC 4 cut(s) 50, 86, 370, 459
TspDTI ATGAA 3 cut(s) 17, 206, 421
TspGWI ACGGA 2 cut(s) 455, 513
TspRI CASTG 4 cut(s) 183, 279, 392, 549
VpaK11BI GGWCC 1 cut(s) 410
VspI ATTAAT 1 cut(s) 693
XagI CCTNNNNNAGG 1 cut(s) 417
XapI RAATTY 1 cut(s) 33
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 398, 437
XspI CTAG 2 cut(s) 126, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.