MD14G1246200.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003141
Physical Location & Seq
Forward (+)
32392146 .. 32393518
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1246200.v1.1.491

Sequence Viewer

Length: 780 bp
ATGAATGAGAAATTTGCAGATCAATTTGTTGCCAGTTGCAGTCCGTACATGATGCACTCGTATGATTATGATAGTGATACAACACCAGAAAGGCAAAGCCAAAGCCAAAGGCAGGCATCTTCTTTATCTGAACCTATCAGCTTATGCCATAGTCCTTCTTCTTCTGCACACTCATCTTCCTCCTTCAAAACCACCTCCACCTATGGAGATGATTCGAGGAAGGGCCTCAACAAAAAATTAGGGAAGAAAAGTAGCAGTTTCGCATACAGGATACGCGACCACGTGAAACTTGGGCACAAGCTGTCAGAAACAGTGAAGGGCAAGTTGAGTTTGGGGGCAAGAATCATTCAAGAGGGTGGGAGGGCCAATATTTTTAAACAGATATTCGGTGTCAGCCAAGGAGAAGAGCTCTTGAAGGCCTCCCAATGCTACCTATCCACCACCGCCGGTCCCATTCCTGGCCTCCTTTTCATTTCTACCCAAAAGCTTGCTTTTTGCAGCGAAAGACCCATCACTCTCCCTTCTCCTCCTGCTTCTGGTCCTGCTGCTGCTGGACAACTGCAACTCCTCAGAACGCATTACAAAGTTCGGATACCAATAAGGAAGATCAAAACAGTGAACCAAAGCGAGAATGTCAACAAACCACAACAGAAGTACATTGAAATAGTTACCCACGATGATTTCGACTTCTGGTTCATGGGATTCTTGCGCTACGAGAAAGCTTTCAGAAATCTTCACAAGGCCCTTTCCATTGCCAACCACAACCAGTCCCACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

260

Amino Acids

28.93

Weight (kDa)

9.61

Isoelectric Point (pI)

59.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 125 - 250 1.2e-11 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 276
AciI CCGC 1 cut(s) 444
AcsI RAATTY 1 cut(s) 11
AcvI CACGTG 1 cut(s) 283
AfaI GTAC 2 cut(s) 47, 656
AfiI CCNNNNNNNGG 3 cut(s) 112, 458, 536
AgsI TTSAA 4 cut(s) 187, 350, 415, 662
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 5 cut(s) 141, 301, 409, 487, 722
AluI AGCT 5 cut(s) 141, 301, 409, 487, 722
Alw21I GWGCWC 1 cut(s) 411
AoxI GGCC 5 cut(s) 223, 363, 417, 460, 741
ApeKI GCWGC 3 cut(s) 498, 545, 548
ApoI RAATTY 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 5 cut(s) 223, 363, 449, 539, 742
AvaII GGWCC 2 cut(s) 449, 539
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 3 cut(s) 510, 532, 535
BccI CCATC 1 cut(s) 518
BciT130I CCWGG 1 cut(s) 459
BciVI GTATCC 2 cut(s) 264, 585
BfaI CTAG 1 cut(s) 778
BfuI GTATCC 2 cut(s) 264, 585
BisI GCNGC 3 cut(s) 499, 546, 549
BlsI GCNGC 3 cut(s) 500, 547, 550
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 449, 539
BmgT120I GGNCC 5 cut(s) 223, 363, 449, 539, 742
BmiI GGNNCC 1 cut(s) 451
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 42, 125
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BsaAI YACGTR 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 458, 536
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 33, 766
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 397
BseLI CCNNNNNNNGG 3 cut(s) 112, 458, 536
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 1 cut(s) 583
BseNI ACTGG 2 cut(s) 33, 766
BseRI GAGGAG 2 cut(s) 516, 557
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 3 cut(s) 510, 532, 535
BsgI GTGCAG 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 5 cut(s) 225, 365, 419, 462, 743
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 1 cut(s) 447
BslFI GGGAC 2 cut(s) 435, 754
BslI CCNNNNNNNGG 3 cut(s) 112, 458, 536
BsmFI GGGAC 2 cut(s) 435, 754
BsnI GGCC 5 cut(s) 225, 365, 419, 462, 743
Bsp1286I GDGCHC 2 cut(s) 297, 411
Bsp143I GATC 2 cut(s) 19, 606
BspACI CCGC 1 cut(s) 444
BspANI GGCC 5 cut(s) 225, 365, 419, 462, 743
BspCNI CTCAG 1 cut(s) 582
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 2 cut(s) 33, 766
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 2 cut(s) 19, 606
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 313, 616
Bst6I CTCTTC 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 283
BstC8I GCNNGC 2 cut(s) 114, 489
BstDEI CTNAG 1 cut(s) 569
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 2 cut(s) 22, 609
BstMBI GATC 2 cut(s) 19, 606
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 457
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 510, 532, 535
BsuI GTATCC 2 cut(s) 264, 585
BsuRI GGCC 5 cut(s) 225, 365, 419, 462, 743
BtsIMutI CAGTG 2 cut(s) 318, 621
Cac8I GCNNGC 2 cut(s) 114, 489
CfoI GCGC 1 cut(s) 711
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 223, 363, 449, 539, 742
Csp6I GTAC 2 cut(s) 46, 655
CviAII CATG 2 cut(s) 49, 697
CviQI GTAC 2 cut(s) 46, 655
DdeI CTNAG 1 cut(s) 569
DpnI GATC 2 cut(s) 21, 608
DpnII GATC 2 cut(s) 19, 606
DraI TTTAAA 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 449, 539
Eco53kI GAGCTC 1 cut(s) 409
Eco72I CACGTG 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 409
EcoO109I RGGNCCY 2 cut(s) 223, 742
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 2 cut(s) 52, 700
FaiI YATR 8 cut(s) 50, 63, 69, 145, 150, 204, 265, 698
FaqI GGGAC 2 cut(s) 435, 754
FatI CATG 2 cut(s) 48, 696
Fnu4HI GCNGC 3 cut(s) 499, 546, 549
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 3 cut(s) 499, 546, 549
FspBI CTAG 1 cut(s) 778
GlaI GCGC 1 cut(s) 710
GluI GCNGC 3 cut(s) 499, 546, 549
HaeIII GGCC 5 cut(s) 225, 365, 419, 462, 743
HapII CCGG 1 cut(s) 447
HhaI GCGC 1 cut(s) 711
Hin1II CATG 2 cut(s) 52, 700
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 2 cut(s) 485, 720
HinfI GANTC 3 cut(s) 212, 342, 702
HpaII CCGG 1 cut(s) 447
Hpy166II GTNNAC 2 cut(s) 619, 637
Hpy188I TCNGA 5 cut(s) 130, 307, 572, 591, 728
Hpy188III TCNNGA 2 cut(s) 350, 412
Hpy8I GTNNAC 2 cut(s) 619, 637
HpyAV CCTTC 6 cut(s) 165, 193, 214, 310, 409, 531
HpyCH4III ACNGT 2 cut(s) 313, 616
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 6 cut(s) 17, 39, 55, 167, 498, 562
HpyF3I CTNAG 1 cut(s) 569
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 2 cut(s) 52, 700
HspAI GCGC 1 cut(s) 709
Kzo9I GATC 2 cut(s) 19, 606
LguI GCTCTTC 1 cut(s) 399
Lsp1109I GCAGC 3 cut(s) 510, 532, 535
LweI GCATC 2 cut(s) 42, 125
MaeI CTAG 1 cut(s) 778
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 667
MalI GATC 2 cut(s) 21, 608
MboI GATC 2 cut(s) 19, 606
MboII GAAGA 8 cut(s) 111, 150, 153, 168, 256, 416, 616, 725
MhlI GDGCHC 2 cut(s) 297, 411
MluCI AATT 3 cut(s) 11, 23, 236
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 60
MspI CCGG 1 cut(s) 447
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 276
NdeII GATC 2 cut(s) 19, 606
NlaIII CATG 2 cut(s) 52, 700
NlaIV GGNNCC 1 cut(s) 451
PceI AGGCCT 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 681
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 3 cut(s) 212, 342, 702
PkrI GCNGC 3 cut(s) 500, 547, 550
PmaCI CACGTG 1 cut(s) 283
PmlI CACGTG 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 283
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 457
PspCI CACGTG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 1 cut(s) 451
PspPI GGNCC 5 cut(s) 223, 363, 449, 539, 742
RsaI GTAC 2 cut(s) 47, 656
RsaNI GTAC 2 cut(s) 46, 655
RseI CAYNNNNRTG 1 cut(s) 60
SacI GAGCTC 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 3 cut(s) 499, 546, 549
Sau3AI GATC 2 cut(s) 19, 606
Sau96I GGNCC 5 cut(s) 223, 363, 449, 539, 742
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 2 cut(s) 297, 411
SfaNI GCATC 2 cut(s) 42, 125
SinI GGWCC 2 cut(s) 449, 539
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 3 cut(s) 11, 23, 236
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 1 cut(s) 444
SspI AATATT 1 cut(s) 370
SspMI CTAG 1 cut(s) 778
SstI GAGCTC 1 cut(s) 411
StuI AGGCCT 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 457
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 2 cut(s) 313, 616
TaiI ACGT 1 cut(s) 285
TaqI TCGA 2 cut(s) 215, 684
TasI AATT 3 cut(s) 11, 23, 236
TatI WGTACW 1 cut(s) 654
TfiI GAWTC 3 cut(s) 212, 342, 702
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 318, 621
TseI GCWGC 3 cut(s) 498, 545, 548
TspDTI ATGAA 3 cut(s) 17, 460, 685
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 2 cut(s) 318, 621
VpaK11BI GGWCC 2 cut(s) 449, 539
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.