Rw7G000200

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003141
Physical Location & Seq
Reverse (-)
205363 .. 206521
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000200.1

Sequence Viewer

Length: 732 bp
ATGAGGGAGAGATTTGGAGATGAATTTGTTGCGAGTCCATATATGAACTCATATGACACTACCCCAGAGAGAGAAGGGATGCAGCAGCAGCACTCTTCTCTATCAGAACCTGTGAGCTTGTGGCACAGCCCATCATCTTCTTCAGTTTCAGAGGGCTCTTCCTCCTTCAAAACCAGTGGTGATTCAAGTTCAAGGAAAGGCCTCAAGAAAAAATTTGGTGACAGGAAAAGCAGCAGTTTTGCATACAGGATCCGCGAGCACGTGAAACTCGGGCCAAAGTTGTCTGAAACTGTCAAGGGAAAGTTGAGTTTGGGGAAAAGAATTATTCAAGAAGGTGGAAGGGGAAATATATTTAAACAGATATTTGGTGTGAATGAAGGTGAAGAGCTGTTGAAGGCCTCACAGTGCTACCTCTCAACTACTGCTGGTCCTATTTCTGGCCTCCTTTTCATTTCTACTGAAAAGCTTGCTTTCTGCAGTGAAAGATCCATCACCGTCCCTTCTGCAACAAATACCAACCAACTTATCAGAACTCCTTACAAGGTTGTGATACCTATGACAAAGATCAAAGTAGCTAGCCCGAGTGAGAATGTGGACAAACCACAACAGAAGTACATAGAGATAGTTACTGATGATGATTTCGAGTTCTGGTTTATGGGATTCTTACGGTATGAAAAAGCTTTGAGAAATCTTCAGAAGGCCATTTCTATGAGTATGAGCAATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

243

Amino Acids

27.21

Weight (kDa)

9.3

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 118 - 235 1.5e-11 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 1 cut(s) 253
AclWI GGATC 3 cut(s) 244, 257, 480
AcsI RAATTY 2 cut(s) 23, 212
AcuI CTGAAG 2 cut(s) 126, 677
AcvI CACGTG 1 cut(s) 262
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 5 cut(s) 169, 186, 192, 329, 394
AluBI AGCT 5 cut(s) 117, 388, 466, 575, 680
AluI AGCT 5 cut(s) 117, 388, 466, 575, 680
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 3 cut(s) 244, 257, 480
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 2 cut(s) 269, 580
AoxI GGCC 5 cut(s) 199, 272, 396, 439, 699
ApeKI GCWGC 4 cut(s) 82, 85, 88, 231
ApoI RAATTY 2 cut(s) 23, 212
AspS9I GGNCC 2 cut(s) 272, 428
AsuHPI GGTGA 4 cut(s) 191, 230, 392, 484
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 2 cut(s) 269, 580
AvaII GGWCC 1 cut(s) 428
BamHI GGATCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 158
BbrPI CACGTG 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 94, 97, 100, 243
BccI CCATC 2 cut(s) 139, 497
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 1 cut(s) 475
BisI GCNGC 4 cut(s) 83, 86, 89, 232
BlsI GCNGC 4 cut(s) 84, 87, 90, 233
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 269, 580
BmgT120I GGNCC 2 cut(s) 272, 428
BmiI GGNNCC 1 cut(s) 251
BmsI GCATC 1 cut(s) 69
BmtI GCTAGC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 1 cut(s) 174
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 174
BseXI GCAGC 4 cut(s) 94, 97, 100, 243
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 5 cut(s) 201, 274, 398, 441, 701
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 2 cut(s) 269, 580
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 5 cut(s) 201, 274, 398, 441, 701
BsoBI CYCGRG 2 cut(s) 269, 580
Bsp1286I GDGCHC 2 cut(s) 158, 261
Bsp143I GATC 3 cut(s) 249, 485, 564
BspACI CCGC 1 cut(s) 253
BspANI GGCC 5 cut(s) 201, 274, 398, 441, 701
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 251
BspMAI CTGCAG 1 cut(s) 479
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 3 cut(s) 244, 257, 480
BspQI GCTCTTC 2 cut(s) 163, 378
BsrI ACTGG 1 cut(s) 174
BssMI GATC 3 cut(s) 249, 485, 564
Bst4CI ACNGT 4 cut(s) 292, 405, 496, 669
Bst6I CTCTTC 3 cut(s) 100, 163, 378
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 3 cut(s) 257, 468, 577
BstF5I GGATG 1 cut(s) 84
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 3 cut(s) 252, 488, 567
BstMBI GATC 3 cut(s) 249, 485, 564
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 94, 97, 100, 243
BstX2I RGATCY 2 cut(s) 249, 485
BstYI RGATCY 2 cut(s) 249, 485
BsuRI GGCC 5 cut(s) 201, 274, 398, 441, 701
BtsCI GGATG 1 cut(s) 84
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 3 cut(s) 181, 410, 484
Cac8I GCNNGC 3 cut(s) 257, 468, 577
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 272, 428
Csp6I GTAC 1 cut(s) 613
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviQI GTAC 1 cut(s) 613
DpnI GATC 3 cut(s) 251, 487, 566
DpnII GATC 3 cut(s) 249, 485, 564
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 100, 163, 378
EarI CTCTTC 3 cut(s) 100, 163, 378
Eco147I AGGCCT 2 cut(s) 201, 398
Eco24I GRGCYC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 126, 677
Eco72I CACGTG 1 cut(s) 262
Eco88I CYCGRG 2 cut(s) 269, 580
EcoT38I GRGCYC 1 cut(s) 158
FaqI GGGAC 1 cut(s) 482
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 83, 86, 89, 232
FokI GGATG 1 cut(s) 91
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 4 cut(s) 83, 86, 89, 232
FspBI CTAG 1 cut(s) 576
GluI GCNGC 4 cut(s) 83, 86, 89, 232
HaeIII GGCC 5 cut(s) 201, 274, 398, 441, 701
HindIII AAGCTT 2 cut(s) 464, 678
HinfI GANTC 3 cut(s) 34, 182, 660
HphI GGTGA 4 cut(s) 191, 230, 392, 484
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 5 cut(s) 106, 151, 286, 530, 696
Hpy188III TCNNGA 3 cut(s) 205, 329, 725
Hpy8I GTNNAC 1 cut(s) 595
HpyAV CCTTC 8 cut(s) 68, 175, 326, 333, 371, 388, 510, 691
HpyCH4III ACNGT 4 cut(s) 292, 405, 496, 669
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 82, 242, 477, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpySE526I ACGT 1 cut(s) 261
Kzo9I GATC 3 cut(s) 249, 485, 564
LguI GCTCTTC 2 cut(s) 163, 378
LpnPI CCDG 8 cut(s) 78, 123, 187, 208, 232, 411, 423, 634
Lsp1109I GCAGC 4 cut(s) 94, 97, 100, 243
LweI GCATC 1 cut(s) 69
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 218, 625
MalI GATC 3 cut(s) 251, 487, 566
MboI GATC 3 cut(s) 249, 485, 564
MboII GAAGA 6 cut(s) 87, 129, 132, 150, 395, 683
MflI RGATCY 2 cut(s) 249, 485
MhlI GDGCHC 2 cut(s) 158, 261
MluCI AATT 3 cut(s) 23, 212, 321
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 6 cut(s) 145, 172, 212, 409, 422, 452
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 707
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 88
NdeI CATATG 1 cut(s) 52
NdeII GATC 3 cut(s) 249, 485, 564
NheI GCTAGC 1 cut(s) 575
NlaIV GGNNCC 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 218
PceI AGGCCT 2 cut(s) 201, 398
PciSI GCTCTTC 2 cut(s) 163, 378
PfeI GAWTC 2 cut(s) 182, 660
PkrI GCNGC 4 cut(s) 84, 87, 90, 233
PleI GAGTC 1 cut(s) 42
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 1 cut(s) 42
Ppu21I YACGTR 1 cut(s) 262
PspCI CACGTG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 251
PspPI GGNCC 2 cut(s) 272, 428
PstI CTGCAG 1 cut(s) 479
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 249, 485
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
RseI CAYNNNNRTG 1 cut(s) 707
SapI GCTCTTC 2 cut(s) 163, 378
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 4 cut(s) 83, 86, 89, 232
Sau3AI GATC 3 cut(s) 249, 485, 564
Sau96I GGNCC 2 cut(s) 272, 428
SchI GAGTC 1 cut(s) 43
SduI GDGCHC 2 cut(s) 158, 261
SfaNI GCATC 1 cut(s) 69
SfcI CTRYAG 1 cut(s) 475
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 3 cut(s) 23, 212, 321
SseBI AGGCCT 2 cut(s) 201, 398
SsiI CCGC 1 cut(s) 253
SspMI CTAG 1 cut(s) 576
StuI AGGCCT 2 cut(s) 201, 398
TaaI ACNGT 4 cut(s) 292, 405, 496, 669
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 642
TasI AATT 3 cut(s) 23, 212, 321
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 2 cut(s) 182, 660
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 181, 410, 484
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 4 cut(s) 82, 85, 88, 231
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 5 cut(s) 36, 59, 390, 439, 687
TspRI CASTG 3 cut(s) 181, 410, 484
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 2 cut(s) 23, 212
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.