AT5G23360

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003141
Physical Location & Seq
Forward (+)
7861737 .. 7862746
1010 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23360.1

Sequence Viewer

Length: 633 bp
ATGACATTGAGCAGAGTTCATCAACAAGTTATTGCATTTCCTGCTGTCAATACTGCTCCGGTGGGTTACTTGCCTGATCCAGCTTCCATCAACAAGCTTCAAATCCCAACTTCTTCAAAGGTTTCGATGCTTCAAAAGAAGAAAACCGATAGCTTCACAAACGGAGCAAGAGATCAGGACAAGTTAGGACCCAAGCTAACCGAAACAGTAAAGAGAAAGCTATCCTTGGGAGCTAAGATCCTTCAAATGGGAGGCTTAGAGAAGATCTATAAGCGACTCTTCAAGGTCTGCGATAAAGAGAAACTCTTCAAGGCGTACCAATGTTACCTATCGACAACAGAAGGTTCCATTGCGGGCCTGCTCTTCATCTCATCAAAGAAGATTGCCTTCTGTAGCGAAAGATCGATCAAGGTGACTTCTCCTCAAGGAGATCTCACTAGGGTTCACTACAAAGTCTCAATCCCCTTGTGCAAGATCAATGGAGTGAACCAGAGTCAGAACACGAAGAAACCATCTCAGAGGTACCTAGAAGTAGTCACAGTCGATAACTATGACTTCTGGTTCATGGGATTCGTGAGCTACCAGAAAGCTTTCAACTGTCTCGAGAAAGCGCTAAACGAGGATGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

210

Amino Acids

23.64

Weight (kDa)

9.58

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 91 - 206 1.4e-11 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 522
AciI CCGC 1 cut(s) 353
AclWI GGATC 2 cut(s) 71, 232
AfaI GTAC 2 cut(s) 317, 524
AfeI AGCGCT 1 cut(s) 612
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 7 cut(s) 101, 117, 134, 245, 283, 310, 595
AluBI AGCT 8 cut(s) 83, 97, 153, 196, 220, 233, 579, 590
AluI AGCT 8 cut(s) 83, 97, 153, 196, 220, 233, 579, 590
Alw26I GTCTC 2 cut(s) 460, 605
AlwI GGATC 2 cut(s) 71, 232
Ama87I CYCGRG 1 cut(s) 602
Aor51HI AGCGCT 1 cut(s) 612
AoxI GGCC 1 cut(s) 355
Asp700I GAANNNNTTC 2 cut(s) 305, 590
Asp718I GGTACC 1 cut(s) 522
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 2 cut(s) 188, 355
AsuHPI GGTGA 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 522
BccI CCATC 2 cut(s) 95, 520
BcoDI GTCTC 2 cut(s) 460, 605
BfaI CTAG 2 cut(s) 438, 527
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 1 cut(s) 614
BglII AGATCT 2 cut(s) 264, 430
Bme18I GGWCC 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 188, 355
BmiI GGNNCC 3 cut(s) 190, 346, 524
BmsI GCATC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 348
BseCI ATCGAT 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 357
BshNI GGYRCC 1 cut(s) 522
BshVI ATCGAT 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 2 cut(s) 460, 605
BsnI GGCC 1 cut(s) 357
BsoBI CYCGRG 1 cut(s) 602
Bsp143I GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
BspACI CCGC 1 cut(s) 353
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 1 cut(s) 529
BspDI ATCGAT 1 cut(s) 404
BspLI GGNNCC 3 cut(s) 190, 346, 524
BspPI GGATC 2 cut(s) 71, 232
BspQI GCTCTTC 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 522
BsrDI GCAATG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 208, 541, 599
Bst6I CTCTTC 3 cut(s) 284, 311, 368
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 355, 359
BstDEI CTNAG 3 cut(s) 234, 256, 516
BstF5I GGATG 1 cut(s) 628
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 8 cut(s) 79, 175, 240, 267, 404, 408, 433, 477
BstMAI GTCTC 2 cut(s) 460, 605
BstMBI GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 391
BstX2I RGATCY 3 cut(s) 237, 264, 430
BstYI RGATCY 3 cut(s) 237, 264, 430
Bsu15I ATCGAT 1 cut(s) 404
BsuRI GGCC 1 cut(s) 357
BsuTUI ATCGAT 1 cut(s) 404
BtsCI GGATG 1 cut(s) 628
Cac8I GCNNGC 2 cut(s) 355, 359
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 2 cut(s) 188, 355
ClaI ATCGAT 1 cut(s) 404
Csp6I GTAC 2 cut(s) 316, 523
CviAII CATG 1 cut(s) 565
CviQI GTAC 2 cut(s) 316, 523
DdeI CTNAG 3 cut(s) 234, 256, 516
DpnI GATC 8 cut(s) 78, 174, 239, 266, 403, 407, 432, 476
DpnII GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
Eam1104I CTCTTC 3 cut(s) 284, 311, 368
EarI CTCTTC 3 cut(s) 284, 311, 368
Eco130I CCWWGG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 188
Eco47III AGCGCT 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 1 cut(s) 568
FaiI YATR 3 cut(s) 270, 552, 566
FalI AAGNNNNNCTT 6 cut(s) 209, 241, 263, 295, 371, 403
FatI CATG 1 cut(s) 564
FauI CCCGC 1 cut(s) 346
FspBI CTAG 2 cut(s) 438, 527
GlaI GCGC 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 1 cut(s) 357
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 613
Hin1II CATG 1 cut(s) 568
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HindIII AAGCTT 2 cut(s) 95, 588
HinfI GANTC 3 cut(s) 276, 493, 570
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 445, 487
Hpy188I TCNGA 2 cut(s) 498, 519
Hpy188III TCNNGA 4 cut(s) 176, 574, 602, 604
Hpy8I GTNNAC 2 cut(s) 445, 487
HpyAV CCTTC 3 cut(s) 251, 335, 397
HpyCH4III ACNGT 3 cut(s) 208, 541, 599
HpyCH4V TGCA 2 cut(s) 35, 471
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 234, 256, 516
Hsp92II CATG 1 cut(s) 568
HspAI GCGC 1 cut(s) 611
KpnI GGTACC 1 cut(s) 526
Kzo9I GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 3 cut(s) 61, 164, 230
LpnPI CCDG 9 cut(s) 54, 72, 87, 93, 161, 371, 503, 544, 596
LweI GCATC 1 cut(s) 117
MaeI CTAG 2 cut(s) 438, 527
MaeIII GTNAC 4 cut(s) 65, 323, 412, 535
MalI GATC 8 cut(s) 78, 174, 239, 266, 403, 407, 432, 476
MboI GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
MboII GAAGA 8 cut(s) 105, 151, 271, 274, 298, 355, 391, 517
MflI RGATCY 3 cut(s) 237, 264, 430
MlyI GAGTC 2 cut(s) 270, 502
MnlI CCTC 4 cut(s) 245, 432, 513, 613
MroXI GAANNNNTTC 2 cut(s) 305, 590
MspI CCGG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
NlaIII CATG 1 cut(s) 568
NlaIV GGNNCC 3 cut(s) 190, 346, 524
NmuCI GTSAC 2 cut(s) 412, 535
PaeR7I CTCGAG 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 368
PdmI GAANNNNTTC 2 cut(s) 305, 590
PfeI GAWTC 1 cut(s) 570
PleI GAGTC 2 cut(s) 270, 501
PpsI GAGTC 2 cut(s) 270, 501
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
PspN4I GGNNCC 3 cut(s) 190, 346, 524
PspPI GGNCC 2 cut(s) 188, 355
PspPPI RGGWCCY 1 cut(s) 188
PsuI RGATCY 3 cut(s) 237, 264, 430
RsaI GTAC 2 cut(s) 317, 524
RsaNI GTAC 2 cut(s) 316, 523
SapI GCTCTTC 1 cut(s) 368
Sau3AI GATC 8 cut(s) 76, 172, 237, 264, 401, 405, 430, 474
Sau96I GGNCC 2 cut(s) 188, 355
SchI GAGTC 2 cut(s) 270, 502
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 391
Sfr274I CTCGAG 1 cut(s) 602
SinI GGWCC 1 cut(s) 188
SlaI CTCGAG 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 423, 602
SmoI CTYRAG 2 cut(s) 423, 602
SsiI CCGC 1 cut(s) 353
SspMI CTAG 2 cut(s) 438, 527
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 3 cut(s) 208, 541, 599
TaqI TCGA 5 cut(s) 125, 332, 404, 543, 603
TfiI GAWTC 1 cut(s) 570
TseFI GTSAC 2 cut(s) 412, 535
Tsp45I GTSAC 2 cut(s) 412, 535
TspDTI ATGAA 3 cut(s) 8, 355, 553
TspGWI ACGGA 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 188
XhoI CTCGAG 1 cut(s) 602
XmnI GAANNNNTTC 2 cut(s) 305, 590
XspI CTAG 2 cut(s) 438, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.