Rw4G037470

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003141
Physical Location & Seq
Reverse (-)
64057147 .. 64058038
892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037470.1

Sequence Viewer

Length: 642 bp
ATGAAGGCTTCATTTCAGGTTCAGCTTGGAGGACAAGTTCTTGGTTATTTCCCAATCAATTCAGCAGCAGCAGCATCATGCACATTTGAGAGGTCCCCTATGAATGCAAAGAGACTCCTGCTACCTGCTCAGCATCATGTTGCTATTCAGTATGATCATGTTCCATCATCTGATCATCGTCGTGATCAAATTGGGTCTTCAAAACGGACGCAAATGAAACTGAGGCCTAAAATCAGTGAGACAGTGAAGGGAAAGTTGAGACTTGGGGCTAAGATTCTTAGAGCAGGTGGAACAATGGAGAAGAAGCTTTTGAAGACTTCTCACTGTTACCTATCAACCACAGCAGGTCCTATTGCAGGCCTCCTTTTCATCTCCACACACAAGATAGCCTTCTGCAGTGACAAGCCAATCAAACTGTCAACATCGCCAAATCCAAATGGACAACTCATCAGAGTCCACTACAAGGTTGTGATCCCACTCAATAAGATAAACAGAGTAAACCAGAGTGAGGATGTGAAGAAGCCATCACAGAAGTACATAGAAATAGTTACTGTGGATAATTATGACTTCTGGTTTATGGGTTTCTTCAATTACCAGAAAGCTCACAAATATCTCCAACAGGCATTAATTTCTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

213

Amino Acids

23.93

Weight (kDa)

10.04

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 100 - 209 8.8e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 275
Acc36I ACCTGC 3 cut(s) 133, 275, 335
AclWI GGATC 1 cut(s) 466
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 3 cut(s) 356, 463, 508
AgsI TTSAA 3 cut(s) 201, 313, 589
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
AluBI AGCT 3 cut(s) 25, 307, 602
AluI AGCT 3 cut(s) 25, 307, 602
Alw26I GTCTC 3 cut(s) 106, 233, 253
AlwI GGATC 1 cut(s) 466
AoxI GGCC 2 cut(s) 224, 358
ApeKI GCWGC 3 cut(s) 65, 68, 71
AseI ATTAAT 1 cut(s) 626
AspS9I GGNCC 2 cut(s) 93, 347
AvaII GGWCC 2 cut(s) 93, 347
BbsI GAAGAC 2 cut(s) 189, 320
BbvI GCAGC 3 cut(s) 77, 80, 83
BccI CCATC 2 cut(s) 172, 532
BclI TGATCA 3 cut(s) 154, 172, 184
BcoDI GTCTC 3 cut(s) 106, 233, 253
BfmI CTRYAG 1 cut(s) 394
BfuAI ACCTGC 3 cut(s) 133, 275, 335
BisI GCNGC 3 cut(s) 66, 69, 72
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 3 cut(s) 67, 70, 73
Bme18I GGWCC 2 cut(s) 93, 347
BmgT120I GGNCC 2 cut(s) 93, 347
BmiI GGNNCC 1 cut(s) 95
BmsI GCATC 2 cut(s) 83, 142
BpiI GAAGAC 2 cut(s) 189, 320
Bpu1102I GCTNAGC 1 cut(s) 129
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 463, 508
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 3 cut(s) 356, 463, 508
BseMII CTCAG 2 cut(s) 143, 212
BseXI GCAGC 3 cut(s) 77, 80, 83
BshFI GGCC 2 cut(s) 226, 360
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 3 cut(s) 356, 463, 508
BsmAI GTCTC 3 cut(s) 106, 233, 253
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 2 cut(s) 226, 360
Bsp143I GATC 4 cut(s) 154, 172, 184, 471
Bsp1720I GCTNAGC 1 cut(s) 129
BspANI GGCC 2 cut(s) 226, 360
BspCNI CTCAG 2 cut(s) 142, 213
BspLI GGNNCC 1 cut(s) 95
BspMAI CTGCAG 1 cut(s) 398
BspMI ACCTGC 3 cut(s) 133, 275, 335
BspPI GGATC 1 cut(s) 466
BssMI GATC 4 cut(s) 154, 172, 184, 471
Bst4CI ACNGT 4 cut(s) 244, 326, 417, 553
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 6 cut(s) 129, 221, 270, 278, 633, 639
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 517
BstKTI GATC 4 cut(s) 157, 175, 187, 474
BstMAI GTCTC 3 cut(s) 106, 233, 253
BstMBI GATC 4 cut(s) 154, 172, 184, 471
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 394
BstV1I GCAGC 3 cut(s) 77, 80, 83
BstV2I GAAGAC 2 cut(s) 189, 320
BsuRI GGCC 2 cut(s) 226, 360
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 4 cut(s) 241, 249, 322, 403
BveI ACCTGC 3 cut(s) 133, 275, 335
Cac8I GCNNGC 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 93, 347
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 535
CviAII CATG 3 cut(s) 78, 137, 158
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 6 cut(s) 129, 221, 270, 278, 633, 639
DpnI GATC 4 cut(s) 156, 174, 186, 473
DpnII GATC 4 cut(s) 154, 172, 184, 471
Eco147I AGGCCT 2 cut(s) 226, 360
Eco47I GGWCC 2 cut(s) 93, 347
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 2 cut(s) 93, 347
FaeI CATG 3 cut(s) 81, 140, 161
FaiI YATR 8 cut(s) 79, 101, 138, 153, 159, 539, 564, 578
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 79
FatI CATG 3 cut(s) 77, 136, 157
FbaI TGATCA 3 cut(s) 154, 172, 184
Fnu4HI GCNGC 3 cut(s) 66, 69, 72
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 3 cut(s) 66, 69, 72
GluI GCNGC 3 cut(s) 66, 69, 72
HaeIII GGCC 2 cut(s) 226, 360
HgaI GACGC 1 cut(s) 217
Hin1II CATG 3 cut(s) 81, 140, 161
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 3 cut(s) 114, 274, 453
Hpy166II GTNNAC 3 cut(s) 420, 457, 499
Hpy188I TCNGA 2 cut(s) 172, 452
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 3 cut(s) 420, 457, 499
Hpy99I CGWCG 1 cut(s) 183
HpyAV CCTTC 2 cut(s) 241, 400
HpyCH4III ACNGT 4 cut(s) 244, 326, 417, 553
HpyCH4V TGCA 4 cut(s) 81, 107, 356, 396
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 6 cut(s) 129, 221, 270, 278, 633, 639
Hsp92II CATG 3 cut(s) 81, 140, 161
Ksp22I TGATCA 3 cut(s) 154, 172, 184
Kzo9I GATC 4 cut(s) 154, 172, 184, 471
Lsp1109I GCAGC 3 cut(s) 77, 80, 83
LweI GCATC 2 cut(s) 83, 142
MaeIII GTNAC 3 cut(s) 326, 398, 547
MalI GATC 4 cut(s) 156, 174, 186, 473
MboI GATC 4 cut(s) 154, 172, 184, 471
MboII GAAGA 5 cut(s) 189, 313, 325, 529, 577
MluCI AATT 5 cut(s) 58, 189, 559, 589, 627
MlyI GAGTC 2 cut(s) 108, 462
MmeI TCCRAC 1 cut(s) 640
MnlI CCTC 5 cut(s) 23, 84, 216, 371, 502
MseI TTAA 1 cut(s) 626
MslI CAYNNNNRTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 4 cut(s) 154, 172, 184, 471
NlaIII CATG 3 cut(s) 81, 140, 161
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 398
PaqCI CACCTGC 1 cut(s) 275
PceI AGGCCT 2 cut(s) 226, 360
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 3 cut(s) 67, 70, 73
PleI GAGTC 2 cut(s) 108, 461
PpsI GAGTC 2 cut(s) 108, 461
PpuMI RGGWCCY 2 cut(s) 93, 347
PshBI ATTAAT 1 cut(s) 626
Psp5II RGGWCCY 2 cut(s) 93, 347
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 2 cut(s) 93, 347
PspPPI RGGWCCY 2 cut(s) 93, 347
PstI CTGCAG 1 cut(s) 398
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 1 cut(s) 626
SatI GCNGC 3 cut(s) 66, 69, 72
Sau3AI GATC 4 cut(s) 154, 172, 184, 471
Sau96I GGNCC 2 cut(s) 93, 347
SchI GAGTC 2 cut(s) 108, 462
SfaNI GCATC 2 cut(s) 83, 142
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 2 cut(s) 93, 347
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 5 cut(s) 58, 189, 559, 589, 627
SseBI AGGCCT 2 cut(s) 226, 360
StuI AGGCCT 2 cut(s) 226, 360
TaaI ACNGT 4 cut(s) 244, 326, 417, 553
TasI AATT 5 cut(s) 58, 189, 559, 589, 627
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 1 cut(s) 626
Tru9I TTAA 1 cut(s) 626
TscAI CASTG 4 cut(s) 241, 249, 329, 403
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 3 cut(s) 65, 68, 71
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 4 cut(s) 17, 116, 230, 358
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 4 cut(s) 241, 249, 329, 403
VpaK11BI GGWCC 2 cut(s) 93, 347
VspI ATTAAT 1 cut(s) 626
XagI CCTNNNNNAGG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.