MD13G1002900.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003141
Physical Location & Seq
Forward (+)
176696 .. 177903
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1002900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGAAGGCCTCACGTCAAGGACAAGTTATCGGAATTCCAATGAACTCGGCAGCAGCCGCCGCAGCTGCGTATTCGTTTAAGAGTGCAGATCATACTGCTGCTATCCAATATCATCATAACATTCCAGCAGCGGCTCCTAATGATCGTGAAATCACTACTGGATCTTCAAAGCTCATCGGACAAAGCAGAGTGCATTCAGTGCTTAACAGGATGAACAAGCTTACCAAGAAGGCTGACAGTTTTGCCCATGGAGTCAAAGAACACGTGAGACTGGGGTCCAAGATCACAGAGACGGTGAAGTGGAAGCTGAGGCTGGGGGCTAAAATTGTGAAAGTAGGAGGGACGTTAGAGAAGGTGTTTAAGCATGCATTCAGCGTCAACGCAGGAGAGAGGCTGCTCAAGGCATCCCAGTGTTATCTGTCGACCACGGCGGGTCCTATGGCAGGCCTCCTCTTCATCTCGACGGACAAGATTGCGTTTTGCAGCGAGAGATCGATCAAACTGCCATCGTCAGATTCAGAGGGACAACAGCTGGTAAGAAGAGTCCACTACAAGGTGGTGATCCCAGTGAACAAGATCAACAGGGTCAACCAGAGCGAGAACGTGAAGAAGCCGTCGCAAAAGTACGTAGAAATAGTTACTGTGGATGACTTTGACTTCTGGTTTATGGGGTTCTTGAATTACCAGAAAACTTTGAAATATCTCCAGCAGGCTATTAATATTCCATCTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

246

Amino Acids

27.07

Weight (kDa)

9.98

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 119 - 239 6.1e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 422
AciI CCGC 4 cut(s) 57, 60, 131, 431
AclWI GGATC 2 cut(s) 169, 556
AcsI RAATTY 1 cut(s) 33
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 2 cut(s) 443, 553
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 3 cut(s) 168, 679, 697
AjiI CACGTC 1 cut(s) 14
AluBI AGCT 6 cut(s) 65, 172, 220, 307, 532, 734
AluI AGCT 6 cut(s) 65, 172, 220, 307, 532, 734
Alw26I GTCTC 2 cut(s) 262, 284
AlwI GGATC 2 cut(s) 169, 556
AoxI GGCC 2 cut(s) 6, 445
ApeKI GCWGC 8 cut(s) 50, 53, 62, 65, 98, 128, 394, 483
ApoI RAATTY 1 cut(s) 33
AseI ATTAAT 1 cut(s) 717
AspS9I GGNCC 2 cut(s) 276, 434
AsuHPI GGTGA 2 cut(s) 307, 571
AvaII GGWCC 2 cut(s) 276, 434
BbrPI CACGTG 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 8 cut(s) 52, 62, 65, 74, 85, 140, 381, 495
BccI CCATC 2 cut(s) 514, 733
BceAI ACGGC 2 cut(s) 444, 598
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BcoDI GTCTC 2 cut(s) 262, 284
Bme18I GGWCC 2 cut(s) 276, 434
BmgBI CACGTC 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 276, 434
BmiI GGNNCC 3 cut(s) 135, 277, 435
BmrI ACTGGG 3 cut(s) 281, 403, 560
BmsI GCATC 1 cut(s) 413
BmuI ACTGGG 3 cut(s) 281, 403, 560
BoxI GACNNNNGTC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 689
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 383, 714
Bsa29I ATCGAT 1 cut(s) 494
BsaAI YACGTR 2 cut(s) 265, 628
BsaJI CCNNGG 2 cut(s) 247, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 553
Bse1I ACTGG 4 cut(s) 163, 276, 409, 566
BseCI ATCGAT 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 247, 426
BseGI GGATG 3 cut(s) 216, 404, 652
BseLI CCNNNNNNNGG 2 cut(s) 443, 553
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 4 cut(s) 163, 276, 409, 566
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 8 cut(s) 52, 62, 65, 74, 85, 140, 381, 495
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 105
BshFI GGCC 2 cut(s) 8, 447
BshVI ATCGAT 1 cut(s) 494
BslFI GGGAC 2 cut(s) 355, 537
BslI CCNNNNNNNGG 2 cut(s) 443, 553
BsmAI GTCTC 2 cut(s) 262, 284
BsmBI CGTCTC 1 cut(s) 284
BsmFI GGGAC 2 cut(s) 355, 537
BsmI GAATGC 2 cut(s) 193, 368
BsnI GGCC 2 cut(s) 8, 447
Bsp143I GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 4 cut(s) 57, 60, 131, 431
BspANI GGCC 2 cut(s) 8, 447
BspCNI CTCAG 1 cut(s) 300
BspDI ATCGAT 1 cut(s) 494
BspLI GGNNCC 3 cut(s) 135, 277, 435
BspPI GGATC 2 cut(s) 169, 556
BsrI ACTGG 4 cut(s) 163, 276, 409, 566
BssECI CCNNGG 2 cut(s) 247, 426
BssMI GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
BssT1I CCWWGG 1 cut(s) 247
Bst4CI ACNGT 3 cut(s) 239, 295, 643
Bst6I CTCTTC 2 cut(s) 458, 535
BstAPI GCANNNNNTGC 1 cut(s) 199
BstBAI YACGTR 2 cut(s) 265, 628
BstC8I GCNNGC 3 cut(s) 366, 445, 711
BstDEI CTNAG 1 cut(s) 308
BstDSI CCRYGG 2 cut(s) 247, 426
BstENI CCTNNNNNAGG 1 cut(s) 441
BstF5I GGATG 3 cut(s) 216, 404, 652
BstKTI GATC 8 cut(s) 91, 145, 164, 285, 494, 498, 564, 579
BstMAI GTCTC 2 cut(s) 262, 284
BstMBI GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
BstMWI GCNNNNNNNGC 5 cut(s) 56, 59, 62, 65, 199
BstNSI RCATGY 1 cut(s) 368
BstPAI GACNNNNGTC 1 cut(s) 274
BstSNI TACGTA 1 cut(s) 628
BstV1I GCAGC 8 cut(s) 52, 62, 65, 74, 85, 140, 381, 495
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 494
BsuRI GGCC 2 cut(s) 8, 447
BsuTUI ATCGAT 1 cut(s) 494
BtgI CCRYGG 2 cut(s) 247, 426
BtrI CACGTC 1 cut(s) 14
BtsCI GGATG 3 cut(s) 216, 404, 652
BtsIMutI CAGTG 3 cut(s) 204, 416, 573
Cac8I GCNNGC 3 cut(s) 366, 445, 711
Cfr13I GGNCC 2 cut(s) 276, 434
ClaI ATCGAT 1 cut(s) 494
CseI GACGC 1 cut(s) 364
Csp6I GTAC 1 cut(s) 625
CviAII CATG 2 cut(s) 248, 365
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 1 cut(s) 308
DpnI GATC 8 cut(s) 90, 144, 163, 284, 493, 497, 563, 578
DpnII GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
Eam1104I CTCTTC 2 cut(s) 458, 535
EarI CTCTTC 2 cut(s) 458, 535
Eco105I TACGTA 1 cut(s) 628
Eco130I CCWWGG 1 cut(s) 247
Eco147I AGGCCT 2 cut(s) 8, 447
Eco47I GGWCC 2 cut(s) 276, 434
Eco72I CACGTG 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 434
EcoRI GAATTC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 284
FaeI CATG 2 cut(s) 251, 368
FaiI YATR 6 cut(s) 93, 117, 249, 366, 440, 668
FaqI GGGAC 2 cut(s) 355, 537
FatI CATG 2 cut(s) 247, 364
FauI CCCGC 1 cut(s) 424
FblI GTMKAC 1 cut(s) 422
FokI GGATG 3 cut(s) 223, 391, 659
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 2 cut(s) 8, 447
HgaI GACGC 1 cut(s) 364
Hin1II CATG 2 cut(s) 251, 368
HincII GTYRAC 3 cut(s) 379, 423, 589
HindII GTYRAC 3 cut(s) 379, 423, 589
HindIII AAGCTT 2 cut(s) 218, 732
HinfI GANTC 3 cut(s) 252, 515, 543
HphI GGTGA 2 cut(s) 307, 571
Hpy166II GTNNAC 5 cut(s) 379, 423, 547, 571, 589
Hpy188I TCNGA 4 cut(s) 32, 179, 514, 520
Hpy188III TCNNGA 3 cut(s) 146, 460, 676
Hpy8I GTNNAC 5 cut(s) 379, 423, 547, 571, 589
Hpy99I CGWCG 2 cut(s) 466, 619
HpyAV CCTTC 2 cut(s) 223, 346
HpyCH4III ACNGT 3 cut(s) 239, 295, 643
HpyCH4IV ACGT 5 cut(s) 13, 264, 344, 603, 627
HpyCH4V TGCA 4 cut(s) 86, 193, 368, 483
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 59, 62, 65, 199
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 5 cut(s) 13, 264, 344, 603, 627
Hsp92II CATG 2 cut(s) 251, 368
Kzo9I GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
LmnI GCTCC 1 cut(s) 139
Lsp1109I GCAGC 8 cut(s) 52, 62, 65, 74, 85, 140, 381, 495
LweI GCATC 1 cut(s) 413
MaeII ACGT 5 cut(s) 13, 264, 344, 603, 627
MaeIII GTNAC 1 cut(s) 637
MalI GATC 8 cut(s) 90, 144, 163, 284, 493, 497, 563, 578
MboI GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
MboII GAAGA 4 cut(s) 156, 445, 552, 619
MflI RGATCY 1 cut(s) 161
MluCI AATT 3 cut(s) 33, 324, 679
MlyI GAGTC 2 cut(s) 261, 552
MnlI CCTC 7 cut(s) 19, 303, 332, 384, 458, 461, 514
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 5 cut(s) 78, 204, 360, 717, 736
MslI CAYNNNNRTG 1 cut(s) 409
MspA1I CMGCKG 3 cut(s) 65, 131, 532
Mva1269I GAATGC 2 cut(s) 193, 368
MwoI GCNNNNNNNGC 5 cut(s) 56, 59, 62, 65, 199
NcoI CCATGG 1 cut(s) 247
NdeII GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
NlaIII CATG 2 cut(s) 251, 368
NlaIV GGNNCC 3 cut(s) 135, 277, 435
NmeAIII GCCGAG 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PceI AGGCCT 2 cut(s) 8, 447
PctI GAATGC 2 cut(s) 193, 368
PfeI GAWTC 1 cut(s) 515
PleI GAGTC 2 cut(s) 260, 551
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 2 cut(s) 260, 551
Ppu21I YACGTR 2 cut(s) 265, 628
PpuMI RGGWCCY 1 cut(s) 434
PshAI GACNNNNGTC 1 cut(s) 274
PshBI ATTAAT 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 434
PspCI CACGTG 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 135, 277, 435
PspPI GGNCC 2 cut(s) 276, 434
PspPPI RGGWCCY 1 cut(s) 434
PsuI RGATCY 1 cut(s) 161
PvuII CAGCTG 2 cut(s) 65, 532
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 409
SalI GTCGAC 1 cut(s) 421
SaqAI TTAA 5 cut(s) 78, 204, 360, 717, 736
Sau3AI GATC 8 cut(s) 88, 142, 161, 282, 491, 495, 561, 576
Sau96I GGNCC 2 cut(s) 276, 434
SchI GAGTC 2 cut(s) 261, 552
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 2 cut(s) 276, 434
SmiMI CAYNNNNRTG 1 cut(s) 409
SmlI CTYRAG 2 cut(s) 398, 729
SmoI CTYRAG 2 cut(s) 398, 729
SnaBI TACGTA 1 cut(s) 628
SphI GCATGC 1 cut(s) 368
Sse9I AATT 3 cut(s) 33, 324, 679
SseBI AGGCCT 2 cut(s) 8, 447
SsiI CCGC 4 cut(s) 57, 60, 131, 431
SspI AATATT 1 cut(s) 721
StuI AGGCCT 2 cut(s) 8, 447
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 3 cut(s) 239, 295, 643
TaiI ACGT 5 cut(s) 16, 267, 347, 606, 630
TaqI TCGA 3 cut(s) 422, 461, 494
TasI AATT 3 cut(s) 33, 324, 679
TauI GCSGC 3 cut(s) 59, 62, 134
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 5 cut(s) 78, 204, 360, 717, 736
Tru9I TTAA 5 cut(s) 78, 204, 360, 717, 736
TscAI CASTG 3 cut(s) 204, 416, 573
TseI GCWGC 8 cut(s) 50, 53, 62, 65, 98, 128, 394, 483
TspDTI ATGAA 4 cut(s) 17, 56, 227, 445
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 3 cut(s) 204, 416, 573
VpaK11BI GGWCC 2 cut(s) 276, 434
VspI ATTAAT 1 cut(s) 717
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 1 cut(s) 33
XceI RCATGY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.