RLG00000005563

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003141
Physical Location & Seq
Forward (+)
68559191 .. 68560423
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005563

Sequence Viewer

Length: 735 bp
ATGAGGGAGAGATTTGGAGATGAATTTGTTGCGAGTCCATATATGAACTCATATGACACTACCCCAGAGAGAGAAGGGCTGCAGCAGCAGCAGCACTCTTCTCTATCAGAACCTGTGAGCTTGTGGCACAGCCCATCATCTTCTTCAGTTTCAGAGGGCTCTTCCTCCTTCAAAACCAGTGGTGATTCAAGTTCAAGGAAAGGCCTCAAGAAAAAATTTGGTGACAGGAAAAGCAGCAGTTTTGCATACAGGATCCGCGAGCACGTGAAACTTGGGCCAAAGTTGTCTGAAACTGTCAAGGGAAAGTTGAGTTTGGGGAAGAGAATTATTCAAGAAGGTGGAAGGGGAAATATATTTAAACAGATATTTGGTGTGAATGAAGGTGAAGAGCTGTTGAAGGCCTCACAGTGCTACCTCTCAACAACTGCTGGTCCTATTTCTGGCCTCCTTTTCATTTCTACTCAAAAGCTTGCCTTCTGCAGTGAAAGATCCATCACTGTCCCTTCTGCAACTAATACCAACCAACTTATCAGAACTCCTTACAAAGTTGTAATACCTATAACAAAGATCAAAATAGCTAGCCAGAGTGAGAATGTGGACAAACCACAACAGAAGTACATAGAGATAGTTACTGATGATGATTTCGAGTTCTGGTTTATGGGATTCTTACGGTATGAAAAAGCTTTGAGAAATCTTCAGAAGGCCGTTTCTATGAGTATGAGCAATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

245

Amino Acids

27.33

Weight (kDa)

9.39

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 119 - 236 5.6e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 258
AciI CCGC 1 cut(s) 256
AclWI GGATC 3 cut(s) 247, 260, 483
AcsI RAATTY 2 cut(s) 23, 215
AcuI CTGAAG 2 cut(s) 129, 680
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 5 cut(s) 172, 189, 195, 332, 397
AluBI AGCT 5 cut(s) 120, 391, 469, 578, 683
AluI AGCT 5 cut(s) 120, 391, 469, 578, 683
Alw21I GWGCWC 1 cut(s) 264
AlwI GGATC 3 cut(s) 247, 260, 483
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 5 cut(s) 202, 275, 399, 442, 702
ApeKI GCWGC 6 cut(s) 79, 82, 85, 88, 91, 234
ApoI RAATTY 2 cut(s) 23, 215
AspS9I GGNCC 2 cut(s) 275, 431
AsuHPI GGTGA 3 cut(s) 194, 233, 395
AsuNHI GCTAGC 1 cut(s) 578
AvaII GGWCC 1 cut(s) 431
BamHI GGATCC 1 cut(s) 252
BanII GRGCYC 1 cut(s) 161
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 6 cut(s) 66, 94, 97, 100, 103, 246
BccI CCATC 2 cut(s) 142, 500
BceAI ACGGC 1 cut(s) 689
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 2 cut(s) 80, 478
BisI GCNGC 6 cut(s) 80, 83, 86, 89, 92, 235
BlsI GCNGC 6 cut(s) 81, 84, 87, 90, 93, 236
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 275, 431
BmiI GGNNCC 1 cut(s) 254
BmtI GCTAGC 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 177
BseXI GCAGC 6 cut(s) 66, 94, 97, 100, 103, 246
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 5 cut(s) 204, 277, 401, 444, 704
BsiHKAI GWGCWC 1 cut(s) 264
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 5 cut(s) 204, 277, 401, 444, 704
Bsp1286I GDGCHC 2 cut(s) 161, 264
Bsp143I GATC 3 cut(s) 252, 488, 567
BspACI CCGC 1 cut(s) 256
BspANI GGCC 5 cut(s) 204, 277, 401, 444, 704
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 254
BspMAI CTGCAG 2 cut(s) 84, 482
BspOI GCTAGC 1 cut(s) 582
BspPI GGATC 3 cut(s) 247, 260, 483
BspQI GCTCTTC 2 cut(s) 166, 381
BsrI ACTGG 1 cut(s) 177
BssMI GATC 3 cut(s) 252, 488, 567
Bst4CI ACNGT 4 cut(s) 295, 408, 499, 672
Bst6I CTCTTC 4 cut(s) 103, 166, 314, 381
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 3 cut(s) 260, 471, 580
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 3 cut(s) 255, 491, 570
BstMBI GATC 3 cut(s) 252, 488, 567
BstMWI GCNNNNNNNGC 3 cut(s) 85, 88, 91
BstSFI CTRYAG 2 cut(s) 80, 478
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 6 cut(s) 66, 94, 97, 100, 103, 246
BstX2I RGATCY 2 cut(s) 252, 488
BstYI RGATCY 2 cut(s) 252, 488
BsuRI GGCC 5 cut(s) 204, 277, 401, 444, 704
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 4 cut(s) 184, 413, 487, 495
Cac8I GCNNGC 3 cut(s) 260, 471, 580
CaiI CAGNNNCTG 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 275, 431
Csp6I GTAC 1 cut(s) 616
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviQI GTAC 1 cut(s) 616
DpnI GATC 3 cut(s) 254, 490, 569
DpnII GATC 3 cut(s) 252, 488, 567
DraI TTTAAA 1 cut(s) 358
Eam1104I CTCTTC 4 cut(s) 103, 166, 314, 381
EarI CTCTTC 4 cut(s) 103, 166, 314, 381
Eco147I AGGCCT 2 cut(s) 204, 401
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 2 cut(s) 129, 680
Eco72I CACGTG 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 161
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 1 cut(s) 485
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 6 cut(s) 80, 83, 86, 89, 92, 235
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 6 cut(s) 80, 83, 86, 89, 92, 235
FspBI CTAG 1 cut(s) 579
GluI GCNGC 6 cut(s) 80, 83, 86, 89, 92, 235
HaeIII GGCC 5 cut(s) 204, 277, 401, 444, 704
HindIII AAGCTT 2 cut(s) 467, 681
HinfI GANTC 3 cut(s) 34, 185, 663
HphI GGTGA 3 cut(s) 194, 233, 395
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 5 cut(s) 109, 154, 289, 533, 699
Hpy188III TCNNGA 3 cut(s) 208, 332, 728
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 9 cut(s) 68, 178, 329, 336, 374, 391, 484, 513, 694
HpyCH4III ACNGT 4 cut(s) 295, 408, 499, 672
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 82, 245, 480, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 88, 91
HpySE526I ACGT 1 cut(s) 264
Kzo9I GATC 3 cut(s) 252, 488, 567
LguI GCTCTTC 2 cut(s) 166, 381
LpnPI CCDG 9 cut(s) 78, 126, 190, 211, 235, 414, 426, 596, 637
Lsp1109I GCAGC 6 cut(s) 66, 94, 97, 100, 103, 246
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 221, 628
MalI GATC 3 cut(s) 254, 490, 569
MboI GATC 3 cut(s) 252, 488, 567
MboII GAAGA 7 cut(s) 90, 132, 135, 153, 331, 398, 686
MflI RGATCY 2 cut(s) 252, 488
MhlI GDGCHC 2 cut(s) 161, 264
MluCI AATT 3 cut(s) 23, 215, 324
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 6 cut(s) 148, 175, 215, 412, 425, 455
MseI TTAA 1 cut(s) 357
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 3 cut(s) 85, 88, 91
NdeI CATATG 1 cut(s) 52
NdeII GATC 3 cut(s) 252, 488, 567
NheI GCTAGC 1 cut(s) 578
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 221
PceI AGGCCT 2 cut(s) 204, 401
PciSI GCTCTTC 2 cut(s) 166, 381
PfeI GAWTC 2 cut(s) 185, 663
PkrI GCNGC 6 cut(s) 81, 84, 87, 90, 93, 236
PleI GAGTC 1 cut(s) 42
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 42
Ppu21I YACGTR 1 cut(s) 265
PspCI CACGTG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 2 cut(s) 275, 431
PstI CTGCAG 2 cut(s) 84, 482
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 252, 488
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
SapI GCTCTTC 2 cut(s) 166, 381
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 6 cut(s) 80, 83, 86, 89, 92, 235
Sau3AI GATC 3 cut(s) 252, 488, 567
Sau96I GGNCC 2 cut(s) 275, 431
SchI GAGTC 1 cut(s) 43
SduI GDGCHC 2 cut(s) 161, 264
SfcI CTRYAG 2 cut(s) 80, 478
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 3 cut(s) 23, 215, 324
SseBI AGGCCT 2 cut(s) 204, 401
SsiI CCGC 1 cut(s) 256
SspMI CTAG 1 cut(s) 579
StuI AGGCCT 2 cut(s) 204, 401
TaaI ACNGT 4 cut(s) 295, 408, 499, 672
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 645
TasI AATT 3 cut(s) 23, 215, 324
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 2 cut(s) 185, 663
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 4 cut(s) 184, 413, 487, 502
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 6 cut(s) 79, 82, 85, 88, 91, 234
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 5 cut(s) 36, 59, 393, 442, 690
TspRI CASTG 4 cut(s) 184, 413, 487, 502
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 2 cut(s) 23, 215
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.