Rh7DG002700

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003141
Physical Location & Seq
Reverse (-)
252920 .. 255242
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG002700.1

Sequence Viewer

Length: 555 bp
ATGAGGGAGAGATTTGGAGATGAATTTGTTGCGAGTCCATATATGAACTCATATGACACTACCCCAGAGAGAGAAGGGCTGCAGCAGCAGCACTCTTCTCTATCAGAACCTGTGAGCTTGTGGCACAGCCCATCATCTTCTTCAGTTTCAGAGGGCTCTTCCTCCTTCAAAACCAGTGGTGATTCAAGTTCAAGGAAAGGCCTCAAGAAAAAATTTGGTGACAGGAAAAGCAGCAGTTTTGCATACAGGATCCGCGAGCACGTGAAACTCGGGCCAAAGTTGTCTGAAACTGTCAAGGGAAAGTTGAGTTTGGGGAAAAGAATTATTCAAGAAGGTGGAAGGGGAAATATATTTAAACAGATATTTGGTGTGAATGAAGGTGAAGAGCTGTTGAAGGCCTCACAGTGCTACCTCTCAACAACTGCTGGTCCTATTTCTGGCCTCCTTTTCATTTCTACTGAAAAGCTTGCTTTCTGCAGTGAAAGATCCATCACCGTCCCTTCTGCAACAAATACCAACCAACTTATCAGAACTCCTTACAAGGTAAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

184

Amino Acids

20.28

Weight (kDa)

9.64

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 118 - 161 5.7e-07 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 1 cut(s) 253
AclWI GGATC 3 cut(s) 244, 257, 480
AcsI RAATTY 2 cut(s) 23, 212
AcuI CTGAAG 1 cut(s) 126
AcvI CACGTG 1 cut(s) 262
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 5 cut(s) 169, 186, 192, 329, 394
AluBI AGCT 3 cut(s) 117, 388, 466
AluI AGCT 3 cut(s) 117, 388, 466
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 3 cut(s) 244, 257, 480
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 4 cut(s) 199, 272, 396, 439
ApeKI GCWGC 5 cut(s) 79, 82, 85, 88, 231
ApoI RAATTY 2 cut(s) 23, 212
AspS9I GGNCC 2 cut(s) 272, 428
AsuHPI GGTGA 4 cut(s) 191, 230, 392, 484
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 428
BamHI GGATCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 158
BbrPI CACGTG 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 66, 94, 97, 100, 243
BccI CCATC 2 cut(s) 139, 497
BfaI CTAG 1 cut(s) 553
BfmI CTRYAG 2 cut(s) 80, 475
BisI GCNGC 5 cut(s) 80, 83, 86, 89, 232
BlsI GCNGC 5 cut(s) 81, 84, 87, 90, 233
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 272, 428
BmiI GGNNCC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 174
BseXI GCAGC 5 cut(s) 66, 94, 97, 100, 243
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 4 cut(s) 201, 274, 398, 441
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 4 cut(s) 201, 274, 398, 441
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 158, 261
Bsp143I GATC 2 cut(s) 249, 485
BspACI CCGC 1 cut(s) 253
BspANI GGCC 4 cut(s) 201, 274, 398, 441
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 251
BspMAI CTGCAG 2 cut(s) 84, 479
BspPI GGATC 3 cut(s) 244, 257, 480
BspQI GCTCTTC 2 cut(s) 163, 378
BsrI ACTGG 1 cut(s) 174
BssMI GATC 2 cut(s) 249, 485
Bst4CI ACNGT 3 cut(s) 292, 405, 496
Bst6I CTCTTC 3 cut(s) 100, 163, 378
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 257, 468
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 2 cut(s) 252, 488
BstMBI GATC 2 cut(s) 249, 485
BstMWI GCNNNNNNNGC 2 cut(s) 85, 88
BstSFI CTRYAG 2 cut(s) 80, 475
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 5 cut(s) 66, 94, 97, 100, 243
BstX2I RGATCY 2 cut(s) 249, 485
BstYI RGATCY 2 cut(s) 249, 485
BsuRI GGCC 4 cut(s) 201, 274, 398, 441
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 3 cut(s) 181, 410, 484
Cac8I GCNNGC 2 cut(s) 257, 468
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 272, 428
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
DpnI GATC 2 cut(s) 251, 487
DpnII GATC 2 cut(s) 249, 485
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 100, 163, 378
EarI CTCTTC 3 cut(s) 100, 163, 378
Eco147I AGGCCT 2 cut(s) 201, 398
Eco24I GRGCYC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 126
Eco72I CACGTG 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 158
FaiI YATR 7 cut(s) 40, 42, 44, 52, 54, 244, 350
FaqI GGGAC 1 cut(s) 482
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 80, 83, 86, 89, 232
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 5 cut(s) 80, 83, 86, 89, 232
FspBI CTAG 1 cut(s) 553
GluI GCNGC 5 cut(s) 80, 83, 86, 89, 232
HaeIII GGCC 4 cut(s) 201, 274, 398, 441
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 2 cut(s) 34, 182
HphI GGTGA 4 cut(s) 191, 230, 392, 484
Hpy188I TCNGA 4 cut(s) 106, 151, 286, 530
Hpy188III TCNNGA 2 cut(s) 205, 329
HpyAV CCTTC 7 cut(s) 68, 175, 326, 333, 371, 388, 510
HpyCH4III ACNGT 3 cut(s) 292, 405, 496
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 82, 242, 477, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 88
HpySE526I ACGT 1 cut(s) 261
Kzo9I GATC 2 cut(s) 249, 485
LguI GCTCTTC 2 cut(s) 163, 378
LpnPI CCDG 7 cut(s) 78, 123, 187, 208, 232, 411, 423
Lsp1109I GCAGC 5 cut(s) 66, 94, 97, 100, 243
MaeI CTAG 1 cut(s) 553
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 218
MalI GATC 2 cut(s) 251, 487
MboI GATC 2 cut(s) 249, 485
MboII GAAGA 5 cut(s) 87, 129, 132, 150, 395
MflI RGATCY 2 cut(s) 249, 485
MhlI GDGCHC 2 cut(s) 158, 261
MluCI AATT 3 cut(s) 23, 212, 321
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 6 cut(s) 145, 172, 212, 409, 422, 452
MseI TTAA 1 cut(s) 354
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 85, 88
NdeI CATATG 1 cut(s) 52
NdeII GATC 2 cut(s) 249, 485
NlaIV GGNNCC 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 218
PceI AGGCCT 2 cut(s) 201, 398
PciSI GCTCTTC 2 cut(s) 163, 378
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 5 cut(s) 81, 84, 87, 90, 233
PleI GAGTC 1 cut(s) 42
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 1 cut(s) 42
Ppu21I YACGTR 1 cut(s) 262
PspCI CACGTG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 251
PspPI GGNCC 2 cut(s) 272, 428
PstI CTGCAG 2 cut(s) 84, 479
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 249, 485
SapI GCTCTTC 2 cut(s) 163, 378
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 5 cut(s) 80, 83, 86, 89, 232
Sau3AI GATC 2 cut(s) 249, 485
Sau96I GGNCC 2 cut(s) 272, 428
SchI GAGTC 1 cut(s) 43
SduI GDGCHC 2 cut(s) 158, 261
SetI ASST 9 cut(s) 112, 119, 264, 337, 382, 390, 414, 468, 546
SfcI CTRYAG 2 cut(s) 80, 475
SinI GGWCC 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 3 cut(s) 23, 212, 321
SseBI AGGCCT 2 cut(s) 201, 398
SsiI CCGC 1 cut(s) 253
SspMI CTAG 1 cut(s) 553
StuI AGGCCT 2 cut(s) 201, 398
TaaI ACNGT 3 cut(s) 292, 405, 496
TaiI ACGT 1 cut(s) 264
TasI AATT 3 cut(s) 23, 212, 321
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 181, 410, 484
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 5 cut(s) 79, 82, 85, 88, 231
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 4 cut(s) 36, 59, 390, 439
TspRI CASTG 3 cut(s) 181, 410, 484
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 2 cut(s) 23, 212
XspI CTAG 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.