FvH4_5g18490

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003141
Physical Location & Seq
Forward (+)
10647812 .. 10649376
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18490.t1

Sequence Viewer

Length: 729 bp
ATGAGGGAGAGATATGGAGAATTTGTAGCGAGTCCATATATGAACTCATATGATACCACACCAGAGAGAGAAGGGCTGCAGCAGCAGCACTCTTCCTTATCTGAACCTGGGAGCTTGTGGCACAGCCCTTCATCTTCTACTGTTTCAGAGAGCTCTTCCTCCTTCAAAACCAGTGGTGATTCGAGTTCAAGGAAAAGCCTCAAGAAAAAATTTGGTGACAGGAAAAGCAGCAGTTTTGCATACAGAATACGCGAGCACGTGAAACTTGGGTCAAAGTTGTCTGAAACTGTCAAGGGAAAGTTGAGTTTGGGGAAAAGAATTATTCAAGAAGGTGGAAGGGGAAATATATTTAAGCAGATATTTGGTGTGAATGAAGGAGAAGAGCTGTTGAAGGCCTCGCAGTGCTACCTATCAACAACTGCTGGTCCTATTTCTGGCCTCCTTTTTATTTCTACTCAAAAGCTTGCATTCTGCAGTGAAAGATCTATCACTGTTCCTTCTGCAACTAATACCACCCAAGTTGTTAGAACTCCCTACAAGGTTGTGATACCTATAACGAAGATCAAAATAGCTAGCCAGAGTGAGAATGAGGATAAACCACAACAGAAGTACATAGAGATAGTTACTGATGATGATTTCGAGTTCTGGTTTATGGGATTCTTACGGTATGAAAAAGCTTTCAGAAATCTTCAGAAGGCCATTTCTATGAGTTTGAGCAATCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

243

Amino Acids

27.15

Weight (kDa)

9.38

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 117 - 234 6.2e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 252
AcsI RAATTY 2 cut(s) 20, 209
AcuI CTGAAG 1 cut(s) 674
AcvI CACGTG 1 cut(s) 259
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 1 cut(s) 434
AgsI TTSAA 4 cut(s) 166, 189, 326, 391
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 6 cut(s) 114, 153, 385, 463, 572, 677
AluI AGCT 6 cut(s) 114, 153, 385, 463, 572, 677
Alw21I GWGCWC 2 cut(s) 155, 258
AoxI GGCC 3 cut(s) 393, 436, 696
ApeKI GCWGC 5 cut(s) 76, 79, 82, 85, 228
ApoI RAATTY 2 cut(s) 20, 209
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 188, 227
AsuNHI GCTAGC 1 cut(s) 572
AvaII GGWCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 155
BbrPI CACGTG 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 155, 258
BbvI GCAGC 5 cut(s) 63, 91, 94, 97, 240
BciT130I CCWGG 1 cut(s) 108
BfaI CTAG 1 cut(s) 573
BfmI CTRYAG 2 cut(s) 77, 472
BglII AGATCT 1 cut(s) 482
BisI GCNGC 5 cut(s) 77, 80, 83, 86, 229
BlsI GCNGC 5 cut(s) 78, 81, 84, 87, 230
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 108
BmtI GCTAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 107
BseLI CCNNNNNNNGG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 171
BseXI GCAGC 5 cut(s) 63, 91, 94, 97, 240
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 395, 438, 698
BsiHKAI GWGCWC 2 cut(s) 155, 258
BslI CCNNNNNNNGG 1 cut(s) 434
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 3 cut(s) 395, 438, 698
Bsp1286I GDGCHC 2 cut(s) 155, 258
Bsp143I GATC 2 cut(s) 482, 561
BspANI GGCC 3 cut(s) 395, 438, 698
BspFNI CGCG 1 cut(s) 252
BspMAI CTGCAG 2 cut(s) 81, 476
BspOI GCTAGC 1 cut(s) 576
BspQI GCTCTTC 2 cut(s) 160, 375
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 2 cut(s) 482, 561
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 4 cut(s) 142, 289, 493, 666
Bst6I CTCTTC 3 cut(s) 97, 160, 375
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 3 cut(s) 254, 465, 574
BstFNI CGCG 1 cut(s) 252
BstKTI GATC 2 cut(s) 485, 564
BstMBI GATC 2 cut(s) 482, 561
BstMWI GCNNNNNNNGC 2 cut(s) 82, 85
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 2 cut(s) 77, 472
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 5 cut(s) 63, 91, 94, 97, 240
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 3 cut(s) 395, 438, 698
BtsI GCAGTG 2 cut(s) 407, 481
BtsIMutI CAGTG 4 cut(s) 178, 407, 481, 489
Cac8I GCNNGC 3 cut(s) 254, 465, 574
Cfr13I GGNCC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 610
CspCI CAANNNNNGTGG 4 cut(s) 154, 189, 502, 537
CviQI GTAC 1 cut(s) 610
DpnI GATC 2 cut(s) 484, 563
DpnII GATC 2 cut(s) 482, 561
Eam1104I CTCTTC 3 cut(s) 97, 160, 375
EarI CTCTTC 3 cut(s) 97, 160, 375
Ecl136II GAGCTC 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 674
Eco72I CACGTG 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 155
FauNDI CATATG 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 77, 80, 83, 86, 229
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 5 cut(s) 77, 80, 83, 86, 229
FspBI CTAG 1 cut(s) 573
GluI GCNGC 5 cut(s) 77, 80, 83, 86, 229
HaeIII GGCC 3 cut(s) 395, 438, 698
HindIII AAGCTT 2 cut(s) 461, 675
HinfI GANTC 3 cut(s) 31, 179, 657
HphI GGTGA 2 cut(s) 188, 227
Hpy188I TCNGA 5 cut(s) 103, 148, 283, 683, 693
Hpy188III TCNNGA 3 cut(s) 202, 326, 722
HpyAV CCTTC 9 cut(s) 65, 138, 172, 323, 330, 368, 385, 507, 688
HpyCH4III ACNGT 4 cut(s) 142, 289, 493, 666
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 5 cut(s) 79, 239, 467, 474, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 85
HpySE526I ACGT 1 cut(s) 258
Kzo9I GATC 2 cut(s) 482, 561
LguI GCTCTTC 2 cut(s) 160, 375
LmnI GCTCC 1 cut(s) 111
LpnPI CCDG 9 cut(s) 75, 93, 120, 184, 205, 408, 420, 590, 631
Lsp1109I GCAGC 5 cut(s) 63, 91, 94, 97, 240
MaeI CTAG 1 cut(s) 573
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 215, 622
MalI GATC 2 cut(s) 484, 563
MboI GATC 2 cut(s) 482, 561
MboII GAAGA 6 cut(s) 84, 126, 147, 392, 571, 680
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 155, 258
MluCI AATT 3 cut(s) 20, 209, 318
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 5 cut(s) 169, 209, 406, 449, 583
MseI TTAA 2 cut(s) 351, 727
MslI CAYNNNNRTG 1 cut(s) 704
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 82, 85
NdeI CATATG 1 cut(s) 49
NdeII GATC 2 cut(s) 482, 561
NheI GCTAGC 1 cut(s) 572
NmuCI GTSAC 1 cut(s) 215
PceI AGGCCT 1 cut(s) 395
PciSI GCTCTTC 2 cut(s) 160, 375
PctI GAATGC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 179, 657
PkrI GCNGC 5 cut(s) 78, 81, 84, 87, 230
PleI GAGTC 1 cut(s) 39
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 1 cut(s) 39
Ppu21I YACGTR 1 cut(s) 259
Psp124BI GAGCTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 106
PspCI CACGTG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 106
PspPI GGNCC 1 cut(s) 425
PstI CTGCAG 2 cut(s) 81, 476
PsuI RGATCY 1 cut(s) 482
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 1 cut(s) 155
SapI GCTCTTC 2 cut(s) 160, 375
SaqAI TTAA 2 cut(s) 351, 727
SatI GCNGC 5 cut(s) 77, 80, 83, 86, 229
Sau3AI GATC 2 cut(s) 482, 561
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 2 cut(s) 155, 258
SfcI CTRYAG 2 cut(s) 77, 472
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 3 cut(s) 20, 209, 318
SseBI AGGCCT 1 cut(s) 395
SspMI CTAG 1 cut(s) 573
SstI GAGCTC 1 cut(s) 155
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 4 cut(s) 142, 289, 493, 666
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 182, 639
TasI AATT 3 cut(s) 20, 209, 318
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 2 cut(s) 179, 657
Tru1I TTAA 2 cut(s) 351, 727
Tru9I TTAA 2 cut(s) 351, 727
TscAI CASTG 4 cut(s) 178, 407, 481, 496
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 5 cut(s) 76, 79, 82, 85, 228
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 4 cut(s) 56, 120, 387, 684
TspRI CASTG 4 cut(s) 178, 407, 481, 496
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 2 cut(s) 20, 209
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.