AT5G10400

Histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000501
Physical Location & Seq
Reverse (-)
3270172 .. 3270957
786 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10400.1

Sequence Viewer

Length: 411 bp
ATGGCTCGTACCAAGCAAACAGCTCGTAAATCCACCGGAGGAAAGGCACCAAGGAAGCAACTCGCGACCAAAGCTGCGAGGAAATCAGCTCCAGCCACCGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACGGTGGCGTTGAGAGAAATCAGGAAGTATCAGAAGAGTACTGAGCTTTTGATCCGTAAGCTTCCGTTCCAGAGATTGGTTCGTGAGATTGCTCAAGATTTCAAGACGGATCTGAGGTTCCAGAGCAGTGCCGTCGCAGCTCTTCAGGAAGCAGCTGAGGCTTATCTTGTTGGGCTCTTTGAAGATACTAATCTCTGTGCGATTCATGCTAAGAGGGTTACGATTATGCCTAAGGATATCCAGCTTGCGAGGAGAATCAGAGGAGAGAGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

136

Amino Acids

15.27

Weight (kDa)

11.29

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 8.7e-39 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09200 AT3G27360 AT5G10390 AT5G10400 AT5G65360
fragaria_vesca FvH4_3g08401 FvH4_3g35371 FvH4_3g39201 FvH4_3g43404 FvH4_5g38881 FvH4_6g43231 FvH4_6g43231
malus_domestica MD00G1200300.v1.1 MD03G1020400.v1.1 MD03G1023100.v1.1 MD05G1308500.v1.1 MD10G1287500.v1.1 MD11G1026900.v1.1
prunus_persica Prupe.4G054300_v2.0.a1 Prupe.6G016200_v2.0.a1 Prupe.6G021100_v2.0.a1 Prupe.6G021700_v2.0.a1
pyrus_communis pycom10g24050 pycom11g01750
rosa_chinensis RchiOBHm_Chr2g0159531 RchiOBHm_Chr5g0009481 RchiOBHm_Chr5g0063481 RchiOBHm_Chr5g0070661 RchiOBHm_Chr5g0077691 RchiOBHm_Chr5g0077731 RchiOBHm_Chr5g0077781 RchiOBHm_Chr5g0077851
rosa_laevigata RLG00000021163 RLG00000031665 RLG00000035639 RLG00000036148 RLG00000036709
rosa_multiflora Rmu_sc0002711.1_g000014 Rmu_sc0002944.1_g000060 Rmu_sc0004168.1_g000003 Rmu_sc0004168.1_g000010 Rmu_sc0004277.1_g000010 Rmu_sc0004277.1_g000017 Rmu_sc0004277.1_g000019 Rmu_sc0005197.1_g000016 Rmu_sc0007856.1_g000002 Rmu_sc0012414.1_g000011 Rmu_sc0016394.1_g000002 Rmu_ssc0000309.1_g000059
rosa_roxburghii Rroxscaffold_1G00004350 Rroxscaffold_1G00010570 Rroxscaffold_1G00017230 Rroxscaffold_2G00090420
rosa_rugosa Rorug02G0476400 Rorug04G0443400 Rorug05G0357600 Rorug05G0407400 Rorug05G0456200 Rorug05G0456600
rosa_samantha Rh2BG556100 Rh2BG556600 Rh2CG526800 Rh2DG565600 Rh5AG510400 Rh5AG510600 Rh5AG510800 Rh5BG070300 Rh5BG431900 Rh5BG481100 Rh5BG532700 Rh5BG532900 Rh5BG533400 Rh5BG533500 Rh5CG082700 Rh5CG455200 Rh5CG505300 Rh5CG556400 Rh5CG556600 Rh5CG556900 Rh5CG557000
rosa_wichuraiana Rw2G044950 Rw5G006900 Rw5G039150 Rw5G043100 Rw5G047360 Rw5G047380 Rw5G047410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 211
AccII CGCG 1 cut(s) 65
AclWI GGATC 2 cut(s) 181, 252
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 2 cut(s) 10, 175
AfiI CCNNNNNNNGG 2 cut(s) 98, 211
AgsI TTSAA 2 cut(s) 238, 317
AjnI CCWGG 1 cut(s) 130
AloI GAACNNNNNNTCC 2 cut(s) 236, 268
AluBI AGCT 8 cut(s) 23, 74, 89, 181, 196, 275, 290, 379
AluI AGCT 8 cut(s) 23, 74, 89, 181, 196, 275, 290, 379
AlwI GGATC 2 cut(s) 181, 252
ApeKI GCWGC 3 cut(s) 74, 272, 287
AxyI CCTNAGG 1 cut(s) 366
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 312
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 61, 284, 299
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 4 cut(s) 5, 39, 250, 284
BciT130I CCWGG 1 cut(s) 132
BisI GCNGC 3 cut(s) 75, 273, 288
BlsI GCNGC 3 cut(s) 76, 274, 289
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 132
BmiI GGNNCC 2 cut(s) 48, 254
BmrFI CCNGG 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 2 cut(s) 35, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 211
Bse21I CCTNAGG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 50
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 98, 211
BseMII CTCAG 3 cut(s) 168, 239, 282
BseRI GAGGAG 3 cut(s) 117, 400, 411
BseXI GCAGC 3 cut(s) 61, 284, 299
Bsh1236I CGCG 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 46
BsiSI CCGG 2 cut(s) 36, 99
BslI CCNNNNNNNGG 2 cut(s) 98, 211
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 186, 244
Bsp68I TCGCGA 1 cut(s) 65
BspCNI CTCAG 3 cut(s) 169, 240, 283
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 48, 254
BspPI GGATC 2 cut(s) 181, 252
BspQI GCTCTTC 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 2 cut(s) 186, 244
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 2 cut(s) 164, 282
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 5 cut(s) 177, 248, 291, 345, 366
BstFNI CGCG 1 cut(s) 65
BstKTI GATC 2 cut(s) 189, 247
BstMBI GATC 2 cut(s) 186, 244
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 130
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 3 cut(s) 61, 284, 299
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
Bsu36I CCTNAGG 1 cut(s) 366
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
BtuMI TCGCGA 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 381
Csp6I GTAC 2 cut(s) 9, 174
CviAII CATG 1 cut(s) 341
CviQI GTAC 2 cut(s) 9, 174
DdeI CTNAG 5 cut(s) 177, 248, 291, 345, 366
DpnI GATC 2 cut(s) 188, 246
DpnII GATC 2 cut(s) 186, 244
Eam1104I CTCTTC 2 cut(s) 164, 282
EarI CTCTTC 2 cut(s) 164, 282
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 263
Eco81I CCTNAGG 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 344
FaiI YATR 2 cut(s) 342, 362
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 3 cut(s) 75, 273, 288
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 3 cut(s) 75, 273, 288
GluI GCNGC 3 cut(s) 75, 273, 288
GsuI CTGGAG 1 cut(s) 75
HapII CCGG 2 cut(s) 36, 99
Hin1II CATG 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 3 cut(s) 123, 337, 390
HpaII CCGG 2 cut(s) 36, 99
Hpy188I TCNGA 3 cut(s) 168, 249, 395
Hpy188III TCNNGA 8 cut(s) 64, 157, 205, 218, 230, 238, 256, 281
Hpy99I CGWCG 1 cut(s) 272
HpyCH4III ACNGT 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 5 cut(s) 177, 248, 291, 345, 366
Hsp92II CATG 1 cut(s) 344
Kzo9I GATC 2 cut(s) 186, 244
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 3 cut(s) 61, 284, 299
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 188, 246
MboI GATC 2 cut(s) 186, 244
MboII GAAGA 4 cut(s) 121, 181, 269, 329
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 312
MnlI CCTC 9 cut(s) 32, 72, 95, 243, 286, 342, 378, 389, 396
MseI TTAA 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 36, 99
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NdeII GATC 2 cut(s) 186, 244
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 48, 254
NruI TCGCGA 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 123, 337, 390
PflMI CCANNNNNTGG 1 cut(s) 211
PfoI TCCNGGA 1 cut(s) 130
PkrI GCNGC 3 cut(s) 76, 274, 289
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 48, 254
PsuI RGATCY 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 290
RruI TCGCGA 1 cut(s) 65
RsaI GTAC 2 cut(s) 10, 175
RsaNI GTAC 2 cut(s) 9, 174
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 3 cut(s) 75, 273, 288
Sau3AI GATC 2 cut(s) 186, 244
ScaI AGTACT 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 312
SetI ASST 9 cut(s) 25, 76, 91, 183, 198, 254, 277, 292, 381
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 139
TatI WGTACW 1 cut(s) 173
TfiI GAWTC 3 cut(s) 123, 337, 390
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 268
TseI GCWGC 3 cut(s) 74, 272, 287
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 4 cut(s) 116, 179, 189, 257
TspRI CASTG 1 cut(s) 268
Van91I CCANNNNNTGG 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.