MD03G1020400.v1.1

Histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000501
Physical Location & Seq
Reverse (-)
1577631 .. 1578041
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1020400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCTCGCACCAAGCAGACTGCTCGCAAGTCCACCGGAGGCAAAGCCCCAAGGAAGCAGCTGGCCACCAAGGCTGCCCGGAAGTCGGCTCCGGCGACCGGAGGAGTGAAGAAGCCCCACCGCTTCAGGCCCGGAACTGTGGCGCTGAGGGAGATCAGGAAGTACCAGAAGAGCACTGAGCTCCTGATCCGGAAGCTTCCGTTCCAGAGGCTCGTGAGGGAGATCGCCCAGGACTTCAAGACCGATCTGAGGTTCCAGAGCAGCGCTGTGGCGGCCCTTCAGGAGGCGGCGGAGGCGTACTTGGTGGGTCTGTTCGAGGATACCAACCTGTGTGCGATTCATGCCAAGAGGGTCACAATCATGCCCAAGGACATCCAGCTCGCTCGTAGGATCAGAGGCGAGAGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

137

Amino Acids

15.27

Weight (kDa)

11.29

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 8.7e-39 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09200 AT3G27360 AT5G10390 AT5G10400 AT5G65360
fragaria_vesca FvH4_3g08401 FvH4_3g35371 FvH4_3g39201 FvH4_3g43404 FvH4_5g38881 FvH4_6g43231 FvH4_6g43231
malus_domestica MD00G1200300.v1.1 MD03G1020400.v1.1 MD03G1023100.v1.1 MD05G1308500.v1.1 MD10G1287500.v1.1 MD11G1026900.v1.1
prunus_persica Prupe.4G054300_v2.0.a1 Prupe.6G016200_v2.0.a1 Prupe.6G021100_v2.0.a1 Prupe.6G021700_v2.0.a1
pyrus_communis pycom10g24050 pycom11g01750
rosa_chinensis RchiOBHm_Chr2g0159531 RchiOBHm_Chr5g0009481 RchiOBHm_Chr5g0063481 RchiOBHm_Chr5g0070661 RchiOBHm_Chr5g0077691 RchiOBHm_Chr5g0077731 RchiOBHm_Chr5g0077781 RchiOBHm_Chr5g0077851
rosa_laevigata RLG00000021163 RLG00000031665 RLG00000035639 RLG00000036148 RLG00000036709
rosa_multiflora Rmu_sc0002711.1_g000014 Rmu_sc0002944.1_g000060 Rmu_sc0004168.1_g000003 Rmu_sc0004168.1_g000010 Rmu_sc0004277.1_g000010 Rmu_sc0004277.1_g000017 Rmu_sc0004277.1_g000019 Rmu_sc0005197.1_g000016 Rmu_sc0007856.1_g000002 Rmu_sc0012414.1_g000011 Rmu_sc0016394.1_g000002 Rmu_ssc0000309.1_g000059
rosa_roxburghii Rroxscaffold_1G00004350 Rroxscaffold_1G00010570 Rroxscaffold_1G00017230 Rroxscaffold_2G00090420
rosa_rugosa Rorug02G0476400 Rorug04G0443400 Rorug05G0357600 Rorug05G0407400 Rorug05G0456200 Rorug05G0456600
rosa_samantha Rh2BG556100 Rh2BG556600 Rh2CG526800 Rh2DG565600 Rh5AG510400 Rh5AG510600 Rh5AG510800 Rh5BG070300 Rh5BG431900 Rh5BG481100 Rh5BG532700 Rh5BG532900 Rh5BG533400 Rh5BG533500 Rh5CG082700 Rh5CG455200 Rh5CG505300 Rh5CG556400 Rh5CG556600 Rh5CG556900 Rh5CG557000
rosa_wichuraiana Rw2G044950 Rw5G006900 Rw5G039150 Rw5G043100 Rw5G047360 Rw5G047380 Rw5G047410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 4 cut(s) 121, 272, 287, 290
AclWI GGATC 2 cut(s) 181, 398
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 2 cut(s) 109, 263
AfaI GTAC 2 cut(s) 164, 299
AfeI AGCGCT 1 cut(s) 265
AfiI CCNNNNNNNGG 4 cut(s) 85, 98, 249, 283
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 4 cut(s) 61, 181, 196, 379
AluI AGCT 4 cut(s) 61, 181, 196, 379
Alw21I GWGCWC 2 cut(s) 176, 183
AlwI GGATC 2 cut(s) 181, 398
Aor13HI TCCGGA 1 cut(s) 189
Aor51HI AGCGCT 1 cut(s) 265
AoxI GGCC 3 cut(s) 63, 128, 273
ApeKI GCWGC 3 cut(s) 58, 74, 261
AspLEI GCGC 2 cut(s) 145, 266
AspS9I GGNCC 2 cut(s) 129, 274
AsuC2I CCSGG 2 cut(s) 79, 132
BalI TGGCCA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 183
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 176, 183
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 61, 70, 273
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 230
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 2 cut(s) 79, 132
BfoI RGCGCY 2 cut(s) 146, 267
BfuI GTATCC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 5 cut(s) 59, 75, 262, 273, 288
BlsI GCNGC 5 cut(s) 60, 76, 263, 274, 289
Bme1390I CCNGG 3 cut(s) 79, 132, 230
BmgT120I GGNCC 2 cut(s) 129, 274
BmiI GGNNCC 2 cut(s) 90, 254
BmrFI CCNGG 3 cut(s) 79, 132, 230
Bpu10I CCTNAGC 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 79, 132
BsaJI CCNNGG 4 cut(s) 50, 69, 228, 366
BsaWI WCCGGW 3 cut(s) 35, 98, 189
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 98, 249, 283
BseAI TCCGGA 1 cut(s) 189
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 4 cut(s) 50, 69, 228, 366
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 4 cut(s) 85, 98, 249, 283
BseMII CTCAG 3 cut(s) 137, 168, 239
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 61, 70, 273
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 65, 130, 275
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 176, 183
BsiSI CCGG 6 cut(s) 36, 79, 92, 99, 132, 190
BslI CCNNNNNNNGG 4 cut(s) 85, 98, 249, 283
BsnI GGCC 3 cut(s) 65, 130, 275
Bsp1286I GDGCHC 2 cut(s) 176, 183
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 5 cut(s) 153, 186, 222, 244, 390
BspACI CCGC 4 cut(s) 121, 272, 287, 290
BspANI GGCC 3 cut(s) 65, 130, 275
BspCNI CTCAG 3 cut(s) 138, 169, 240
BspEI TCCGGA 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 90, 254
BspPI GGATC 2 cut(s) 181, 398
BspQI GCTCTTC 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 50, 69, 228, 366
BssMI GATC 5 cut(s) 153, 186, 222, 244, 390
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 50, 69, 366
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 7, 25, 63, 381
BstDEI CTNAG 3 cut(s) 146, 177, 248
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 372
BstH2I RGCGCY 2 cut(s) 146, 267
BstHHI GCGC 2 cut(s) 145, 266
BstKTI GATC 5 cut(s) 156, 189, 225, 247, 393
BstMBI GATC 5 cut(s) 153, 186, 222, 244, 390
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 77, 130, 228
BstV1I GCAGC 3 cut(s) 61, 70, 273
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 3 cut(s) 65, 130, 275
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 4 cut(s) 7, 25, 63, 381
CfoI GCGC 2 cut(s) 145, 266
Cfr13I GGNCC 2 cut(s) 129, 274
Csp6I GTAC 2 cut(s) 163, 298
CviAII CATG 2 cut(s) 341, 361
CviQI GTAC 2 cut(s) 163, 298
DdeI CTNAG 3 cut(s) 146, 177, 248
DpnI GATC 5 cut(s) 155, 188, 224, 246, 392
DpnII GATC 5 cut(s) 153, 186, 222, 244, 390
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 3 cut(s) 50, 69, 366
Eco24I GRGCYC 1 cut(s) 183
Eco47III AGCGCT 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 109, 263
EcoICRI GAGCTC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 3 cut(s) 50, 69, 366
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 3 cut(s) 50, 69, 366
FaeI CATG 2 cut(s) 344, 364
FaiI YATR 2 cut(s) 342, 362
FatI CATG 2 cut(s) 340, 360
Fnu4HI GCNGC 5 cut(s) 59, 75, 262, 273, 288
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 5 cut(s) 59, 75, 262, 273, 288
GlaI GCGC 2 cut(s) 144, 265
GluI GCNGC 5 cut(s) 59, 75, 262, 273, 288
HaeII RGCGCY 2 cut(s) 146, 267
HaeIII GGCC 3 cut(s) 65, 130, 275
HapII CCGG 6 cut(s) 36, 79, 92, 99, 132, 190
HhaI GCGC 2 cut(s) 145, 266
Hin1II CATG 2 cut(s) 344, 364
Hin6I GCGC 2 cut(s) 143, 264
HinP1I GCGC 2 cut(s) 143, 264
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 1 cut(s) 337
HpaII CCGG 6 cut(s) 36, 79, 92, 99, 132, 190
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 249, 395
Hpy188III TCNNGA 8 cut(s) 157, 184, 190, 205, 214, 238, 256, 281
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 3 cut(s) 146, 177, 248
Hsp92II CATG 2 cut(s) 344, 364
HspAI GCGC 2 cut(s) 143, 264
Kpn2I TCCGGA 1 cut(s) 189
Kzo9I GATC 5 cut(s) 153, 186, 222, 244, 390
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 94, 186
Lsp1109I GCAGC 3 cut(s) 61, 70, 273
MaeIII GTNAC 1 cut(s) 352
MalI GATC 5 cut(s) 155, 188, 224, 246, 392
MboI GATC 5 cut(s) 153, 186, 222, 244, 390
MboII GAAGA 2 cut(s) 121, 181
MhlI GDGCHC 2 cut(s) 176, 183
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
Mox20I TGGCCA 1 cut(s) 65
MroI TCCGGA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 65
MseI TTAA 1 cut(s) 409
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 6 cut(s) 36, 79, 92, 99, 132, 190
MspR9I CCNGG 3 cut(s) 79, 132, 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NciI CCSGG 2 cut(s) 79, 132
NdeII GATC 5 cut(s) 153, 186, 222, 244, 390
NlaIII CATG 2 cut(s) 344, 364
NlaIV GGNNCC 2 cut(s) 90, 254
NmuCI GTSAC 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 1 cut(s) 337
PkrI GCNGC 5 cut(s) 60, 76, 263, 274, 289
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 90, 254
PspPI GGNCC 2 cut(s) 129, 274
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 2 cut(s) 164, 299
RsaNI GTAC 2 cut(s) 163, 298
RseI CAYNNNNRTG 1 cut(s) 359
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 5 cut(s) 59, 75, 262, 273, 288
Sau3AI GATC 5 cut(s) 153, 186, 222, 244, 390
Sau96I GGNCC 2 cut(s) 129, 274
ScrFI CCNGG 3 cut(s) 79, 132, 230
SduI GDGCHC 2 cut(s) 176, 183
SetI ASST 6 cut(s) 63, 183, 198, 254, 330, 381
SmiMI CAYNNNNRTG 1 cut(s) 359
SsiI CCGC 4 cut(s) 121, 272, 287, 290
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 77, 130, 228
StyI CCWWGG 3 cut(s) 50, 69, 366
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 315
TaqII GACCGA 1 cut(s) 257
TauI GCSGC 2 cut(s) 275, 290
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 3 cut(s) 58, 74, 261
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.