AT5G13200

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004816
Physical Location & Seq
Forward (+)
4206738 .. 4208289
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13200.2

Sequence Viewer

Length: 819 bp
ATGACAGGATCACAAGAAGACCAACCTAAGATCATCATTGATCAAGAGCAACCCAAAACTCTAGAAACAGAGCACCAACCAGAACCTTCTTCATCGTCTCCGGATCAGAAGAAATGGGGTACTCACGTGATGGGAGCTCCGGCAGCTCCAGTTGCTCATCCCGATAACCAACAGGCGGCGGCGTGGGTCGCTGGAGATAACCAGCAGACGCAGTACCAACCGTACGTCATCTACTCTCCTGTCGAACATCCAACAACTAACAACCCTCTCGAGCCAGTCATCGGAATGTTCCATACCTGGAGTCGCAAGGCAGAAACCGTCGCACGTAACCTCTGGCACAATCTGAAGACAGGACCGTCTATGTCGGAAACGGCGTGGGGGAAGGTTAATTTGACGGCCAAAGCGATAACAAAAGGAGGATTCGAGTCGCTTTTCAGACAGATTTTCGGAACAGAGCCAAACGAGACGCTGAAGAAAACTTTCGCTTGTTATCTCTCGACGACGACAGGTCCTGTTGCTGGAACTGTCTATCTTTCGAATGCTCGTGTCGCTTTTTGTAGCGATCGTCCTCTGTACTTCACAGCACCTTCTGGTCAAGAATCTTGGAGCTACTACAGGGTGGTTGTACCTTTGGCGAATGTAGCGACGGTGAATCCGGTGGTGGTGAAAGAGACTCCACCTGAGAAGTATATTCAGTTGACGACGGTGGATGGTCATGACTTCTGGTTCATGGGTTTTGTGAATTATGAGAAGGCTACGCATCATCTGCTGACCAGTGTCTCCGATTTTCAAACCGCACACGGCTCTGTGTCTGGTTAA

Protein Analysis

272

Amino Acids

30.07

Weight (kDa)

6.05

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 144 - 190 7.6e-10 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 355
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 3 cut(s) 176, 179, 795
AclWI GGATC 2 cut(s) 16, 111
AcoI YGGCCR 1 cut(s) 396
AcuI CTGAAG 2 cut(s) 365, 491
AcvI CACGTG 1 cut(s) 127
AfaI GTAC 5 cut(s) 121, 215, 224, 575, 627
AfiI CCNNNNNNNGG 3 cut(s) 175, 281, 518
AgsI TTSAA 1 cut(s) 791
AhdI GACNNNNNGTC 1 cut(s) 507
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 3 cut(s) 137, 146, 609
AluI AGCT 3 cut(s) 137, 146, 609
Alw21I GWGCWC 2 cut(s) 75, 139
Alw26I GTCTC 4 cut(s) 102, 458, 665, 784
AlwI GGATC 2 cut(s) 16, 111
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 269
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 1 cut(s) 396
ApeKI GCWGC 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 353, 509
AsuHPI GGTGA 2 cut(s) 661, 676
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 2 cut(s) 353, 509
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BanII GRGCYC 1 cut(s) 139
BauI CACGAG 1 cut(s) 543
BbrPI CACGTG 1 cut(s) 127
BbsI GAAGAC 2 cut(s) 24, 353
Bbv12I GWGCWC 2 cut(s) 75, 139
BbvI GCAGC 1 cut(s) 155
BccI CCATC 2 cut(s) 124, 704
BceAI ACGGC 3 cut(s) 387, 411, 817
BciT130I CCWGG 1 cut(s) 298
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 4 cut(s) 102, 458, 665, 784
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 613
BisI GCNGC 3 cut(s) 144, 177, 180
BlsI GCNGC 3 cut(s) 145, 178, 181
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 2 cut(s) 353, 509
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 353, 509
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 769
BoxI GACNNNNGTC 1 cut(s) 776
BpiI GAAGAC 2 cut(s) 24, 353
BpmI CTGGAG 3 cut(s) 132, 213, 319
Bpu14I TTCGAA 1 cut(s) 536
BsaAI YACGTR 2 cut(s) 127, 326
BsaWI WCCGGW 2 cut(s) 100, 655
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 281, 518
Bse1I ACTGG 3 cut(s) 149, 275, 774
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 1 cut(s) 298
BseGI GGATG 3 cut(s) 157, 247, 715
BseLI CCNNNNNNNGG 3 cut(s) 175, 281, 518
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 3 cut(s) 149, 275, 774
BseXI GCAGC 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 75, 139
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 3 cut(s) 101, 140, 656
BsiWI CGTACG 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 175, 281, 518
BsmAI GTCTC 4 cut(s) 102, 458, 665, 784
BsmBI CGTCTC 2 cut(s) 102, 458
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 75, 139
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 5 cut(s) 8, 30, 40, 103, 562
BspACI CCGC 3 cut(s) 176, 179, 795
BspANI GGCC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 673
BspEI TCCGGA 1 cut(s) 100
BspHI TCATGA 1 cut(s) 715
BspPI GGATC 2 cut(s) 16, 111
BspT104I TTCGAA 1 cut(s) 536
BsrI ACTGG 3 cut(s) 149, 275, 774
BssMI GATC 5 cut(s) 8, 30, 40, 103, 562
BssSI CACGAG 1 cut(s) 543
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 6 cut(s) 222, 319, 357, 526, 649, 706
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 2 cut(s) 127, 326
BstBI TTCGAA 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 27, 681
BstF5I GGATG 3 cut(s) 157, 247, 715
BstKTI GATC 5 cut(s) 11, 33, 43, 106, 565
BstMAI GTCTC 4 cut(s) 102, 458, 665, 784
BstMBI GATC 5 cut(s) 8, 30, 40, 103, 562
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 6 cut(s) 143, 152, 188, 548, 641, 766
BstNI CCWGG 1 cut(s) 298
BstPAI GACNNNNGTC 1 cut(s) 776
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 613
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 2 cut(s) 24, 353
BsuRI GGCC 1 cut(s) 398
BtsCI GGATG 3 cut(s) 157, 247, 715
BtsIMutI CAGTG 1 cut(s) 781
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 1 cut(s) 715
Cfr13I GGNCC 2 cut(s) 353, 509
CseI GACGC 2 cut(s) 217, 475
Csp6I GTAC 5 cut(s) 120, 214, 223, 574, 626
CviAII CATG 2 cut(s) 716, 730
CviJI RGCY 8 cut(s) 137, 146, 274, 398, 457, 609, 755, 804
CviKI_1 RGCY 8 cut(s) 137, 146, 274, 398, 457, 609, 755, 804
CviQI GTAC 5 cut(s) 120, 214, 223, 574, 626
DdeI CTNAG 2 cut(s) 27, 681
DpnI GATC 5 cut(s) 10, 32, 42, 105, 564
DpnII GATC 5 cut(s) 8, 30, 40, 103, 562
DrdI GACNNNNNNGTC 1 cut(s) 355
DriI GACNNNNNGTC 1 cut(s) 507
DseDI GACNNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 396
Eam1105I GACNNNNNGTC 1 cut(s) 507
Ecl136II GAGCTC 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 353, 509
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 365, 491
Eco72I CACGTG 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 139
Esp3I CGTCTC 2 cut(s) 102, 458
FaeI CATG 2 cut(s) 719, 733
FaiI YATR 6 cut(s) 294, 362, 690, 717, 731, 747
FatI CATG 2 cut(s) 715, 729
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 144, 177, 180
FokI GGATG 3 cut(s) 144, 234, 722
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 3 cut(s) 144, 177, 180
FspBI CTAG 1 cut(s) 62
GluI GCNGC 3 cut(s) 144, 177, 180
GsuI CTGGAG 3 cut(s) 132, 213, 319
HaeIII GGCC 1 cut(s) 398
HapII CCGG 3 cut(s) 101, 140, 656
HgaI GACGC 2 cut(s) 217, 475
Hin1II CATG 2 cut(s) 719, 733
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HinfI GANTC 6 cut(s) 301, 420, 425, 599, 652, 673
HpaII CCGG 3 cut(s) 101, 140, 656
HphI GGTGA 2 cut(s) 661, 676
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 7 cut(s) 108, 284, 345, 367, 437, 449, 784
Hpy188III TCNNGA 8 cut(s) 44, 62, 101, 161, 269, 496, 596, 716
Hpy8I GTNNAC 1 cut(s) 699
Hpy99I CGWCG 5 cut(s) 323, 502, 505, 649, 706
HpyAV CCTTC 4 cut(s) 96, 376, 597, 745
HpyCH4III ACNGT 6 cut(s) 222, 319, 357, 526, 649, 706
HpyCH4IV ACGT 3 cut(s) 126, 225, 325
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 152, 188, 548, 641, 766
HpyF3I CTNAG 2 cut(s) 27, 681
HpySE526I ACGT 3 cut(s) 126, 225, 325
Hsp92II CATG 2 cut(s) 719, 733
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 5 cut(s) 8, 30, 40, 103, 562
LmnI GCTCC 4 cut(s) 134, 142, 151, 606
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 62
MaeII ACGT 3 cut(s) 126, 225, 325
MaeIII GTNAC 1 cut(s) 326
MalI GATC 5 cut(s) 10, 32, 42, 105, 564
MboI GATC 5 cut(s) 8, 30, 40, 103, 562
MboII GAAGA 5 cut(s) 29, 81, 121, 358, 484
MhlI GDGCHC 2 cut(s) 75, 139
MluCI AATT 2 cut(s) 388, 742
MlyI GAGTC 3 cut(s) 310, 434, 667
MmeI TCCRAC 2 cut(s) 275, 345
MnlI CCTC 4 cut(s) 276, 341, 410, 579
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 2 cut(s) 387, 817
MslI CAYNNNNRTG 1 cut(s) 284
MspI CCGG 3 cut(s) 101, 140, 656
MspR9I CCNGG 1 cut(s) 298
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 6 cut(s) 143, 152, 188, 548, 641, 766
NdeII GATC 5 cut(s) 8, 30, 40, 103, 562
NlaIII CATG 2 cut(s) 719, 733
NspV TTCGAA 1 cut(s) 536
PaeR7I CTCGAG 1 cut(s) 269
PagI TCATGA 1 cut(s) 715
PcsI WCGNNNNNNNCGW 2 cut(s) 371, 401
PctI GAATGC 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 420, 599, 652
Pfl23II CGTACG 1 cut(s) 222
PkrI GCNGC 3 cut(s) 145, 178, 181
Ple19I CGATCG 1 cut(s) 565
PleI GAGTC 3 cut(s) 309, 433, 667
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 3 cut(s) 309, 433, 667
Ppu21I YACGTR 2 cut(s) 127, 326
PpuMI RGGWCCY 1 cut(s) 509
PshAI GACNNNNGTC 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 296
PspCI CACGTG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 296
PspLI CGTACG 1 cut(s) 222
PspPI GGNCC 2 cut(s) 353, 509
PspPPI RGGWCCY 1 cut(s) 509
PstNI CAGNNNCTG 1 cut(s) 512
PvuI CGATCG 1 cut(s) 565
RsaI GTAC 5 cut(s) 121, 215, 224, 575, 627
RsaNI GTAC 5 cut(s) 120, 214, 223, 574, 626
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 387, 817
SatI GCNGC 3 cut(s) 144, 177, 180
Sau3AI GATC 5 cut(s) 8, 30, 40, 103, 562
Sau96I GGNCC 2 cut(s) 353, 509
SchI GAGTC 3 cut(s) 310, 434, 667
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 2 cut(s) 75, 139
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 1 cut(s) 613
Sfr274I CTCGAG 1 cut(s) 269
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 2 cut(s) 353, 509
SlaI CTCGAG 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 2 cut(s) 388, 742
SsiI CCGC 3 cut(s) 176, 179, 795
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 6 cut(s) 222, 319, 357, 526, 649, 706
TaiI ACGT 3 cut(s) 129, 228, 328
TaqI TCGA 5 cut(s) 243, 270, 423, 497, 536
TasI AATT 2 cut(s) 388, 742
TatI WGTACW 1 cut(s) 573
TauI GCSGC 2 cut(s) 179, 182
TfiI GAWTC 3 cut(s) 420, 599, 652
Tru1I TTAA 2 cut(s) 387, 817
Tru9I TTAA 2 cut(s) 387, 817
TscAI CASTG 1 cut(s) 781
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 81, 718
TspRI CASTG 1 cut(s) 781
VpaK11BI GGWCC 2 cut(s) 353, 509
XbaI TCTAGA 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.