Rh4BG296300

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004816
Physical Location & Seq
Forward (+)
46804851 .. 46808027
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG296300.1

Sequence Viewer

Length: 879 bp
ATGTCGAGCAGACCGGAAGACCAGCTTTCTCCCCGGGAGTCTTATCCTCCAACCACAACCGTCCCCGACATTCACGCCTCGCAACCACCGCCGCCGCCTCCGCCGTCTGAGGAGGACGCCAAGAAATGGGGGACCCACATAATGGGAACTCCCGCAGCTCCGAACGTCCACCCGGATAACCAGAAAGCTGCTCTATGGAAAGCCGCCGACCACCAGCAAATCCCGCAGCAACCGCTCGAAACACAACAGCAACAACTGCCGTACGTTCAGTACTCTCCGATCAACAAGCCGACCAACAACCCCTTCGAGCCGGTGATCAACCTCTTCAACTCGTGGGGGAACAAGGCCGAGACCATCGCCCGGAACATCTGGCACAACCTCAAAACAGGGCCCTCTGTTTCCGAGACCGCCTGGGGAAAAGTGAACTTGACGGCCAAGGCGATCACGAAAGGCGGGTTTGAGTCTCTGTTTAAGCAGATTTTCGCTACTGATCCCAACGAGAGGTTGAAGAAGACTTTCGCTTGCTATCTTTCCACTTCTACCGGACCTGTGGCCGGGACTCTGTATCTGTCGACGGCAAGAATCGGTTTTTGCAGCGACCGGCCGTTGACTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATACCTATGACGAATGTAGGGACAGTCAATCCTGTGGTGATTACGGAGACCCCGACGCCGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTCGAGAAAGCTTCGCATCACGTACTAGACAGTGTGTCGAATTTCAGAGCAGTTGGGACCAATGAAGTGCAACAAGTGCAACCTGTTGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

292

Amino Acids

32.34

Weight (kDa)

6.71

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 156 - 265 1.4e-14 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 126, 142
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 572
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 3 cut(s) 432, 552, 602
AcsI RAATTY 1 cut(s) 823
AcuI CTGAAG 1 cut(s) 716
AcyI GRCGYC 2 cut(s) 117, 716
AfaI GTAC 4 cut(s) 263, 272, 728, 807
AfiI CCNNNNNNNGG 6 cut(s) 126, 142, 360, 501, 554, 718
AgsI TTSAA 2 cut(s) 328, 508
AhdI GACNNNNNGTC 1 cut(s) 817
AjnI CCWGG 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 5 cut(s) 25, 158, 188, 645, 794
AluI AGCT 5 cut(s) 25, 158, 188, 645, 794
Alw26I GTCTC 4 cut(s) 344, 398, 468, 701
AlwI GGATC 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 5 cut(s) 345, 389, 432, 552, 602
ApaI GGGCCC 1 cut(s) 393
ApeKI GCWGC 4 cut(s) 155, 188, 226, 594
ApoI RAATTY 1 cut(s) 823
Asp700I GAANNNNTTC 1 cut(s) 515
AspS9I GGNCC 5 cut(s) 132, 389, 390, 545, 840
AsuC2I CCSGG 5 cut(s) 34, 35, 173, 361, 556
AsuHPI GGTGA 4 cut(s) 325, 667, 709, 733
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 3 cut(s) 132, 545, 840
BaeGI GKGCMC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 393
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 24, 518
BbvI GCAGC 4 cut(s) 167, 175, 238, 606
BccI CCATC 2 cut(s) 362, 743
BceAI ACGGC 5 cut(s) 88, 244, 447, 589, 591
BciT130I CCWGG 1 cut(s) 412
BclI TGATCA 1 cut(s) 315
BcnI CCSGG 5 cut(s) 34, 35, 173, 361, 556
BcoDI GTCTC 4 cut(s) 344, 398, 468, 701
BfaI CTAG 1 cut(s) 809
BisI GCNGC 7 cut(s) 92, 95, 156, 189, 204, 227, 595
BlsI GCNGC 7 cut(s) 93, 96, 157, 190, 205, 228, 596
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 6 cut(s) 34, 35, 173, 361, 412, 556
Bme18I GGWCC 3 cut(s) 132, 545, 840
BmeRI GACNNNNNGTC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 132, 389, 390, 545, 840
BmiI GGNNCC 4 cut(s) 133, 134, 391, 841
BmrFI CCNGG 6 cut(s) 34, 35, 173, 361, 412, 556
BmsI GCATC 1 cut(s) 808
BpiI GAAGAC 2 cut(s) 24, 518
BpuMI CCSGG 5 cut(s) 34, 35, 173, 361, 556
BsaAI YACGTR 1 cut(s) 805
BsaHI GRCGYC 2 cut(s) 117, 716
BsaI GGTCTC 3 cut(s) 344, 398, 701
BsaJI CCNNGG 4 cut(s) 32, 33, 411, 435
BsaWI WCCGGW 2 cut(s) 13, 542
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 142, 360, 501, 554, 718
Bse118I RCCGGY 2 cut(s) 310, 600
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 4 cut(s) 32, 33, 411, 435
BseLI CCNNNNNNNGG 6 cut(s) 126, 142, 360, 501, 554, 718
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 125
BseSI GKGCMC 1 cut(s) 393
BseX3I CGGCCG 1 cut(s) 602
BseXI GCAGC 4 cut(s) 167, 175, 238, 606
Bsh1285I CGRYCG 2 cut(s) 601, 605
BshFI GGCC 5 cut(s) 347, 391, 434, 554, 604
BsiEI CGRYCG 2 cut(s) 601, 605
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 9 cut(s) 14, 34, 173, 311, 361, 543, 555, 601, 719
BsiWI CGTACG 1 cut(s) 261
BslFI GGGAC 5 cut(s) 47, 145, 571, 694, 853
BslI CCNNNNNNNGG 6 cut(s) 126, 142, 360, 501, 554, 718
BsmAI GTCTC 4 cut(s) 344, 398, 468, 701
BsmFI GGGAC 5 cut(s) 47, 145, 571, 694, 853
BsnI GGCC 5 cut(s) 347, 391, 434, 554, 604
Bso31I GGTCTC 3 cut(s) 344, 398, 701
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 4 cut(s) 279, 315, 441, 490
BspANI GGCC 5 cut(s) 347, 391, 434, 554, 604
BspCNI CTCAG 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 133, 134, 391, 841
BspPI GGATC 1 cut(s) 485
BspTNI GGTCTC 3 cut(s) 344, 398, 701
BsrBI CCGCTC 1 cut(s) 235
BsrFI RCCGGY 2 cut(s) 310, 600
BssAI RCCGGY 2 cut(s) 310, 600
BssECI CCNNGG 4 cut(s) 32, 33, 411, 435
BssMI GATC 4 cut(s) 279, 315, 441, 490
BssNI GRCGYC 2 cut(s) 117, 716
BssSI CACGAG 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 3 cut(s) 61, 685, 815
Bst6I CTCTTC 1 cut(s) 329
BstACI GRCGYC 2 cut(s) 117, 716
BstAPI GCANNNNNTGC 2 cut(s) 256, 859
BstBAI YACGTR 1 cut(s) 805
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 1 cut(s) 108
BstKTI GATC 4 cut(s) 282, 318, 444, 493
BstMAI GTCTC 4 cut(s) 344, 398, 468, 701
BstMBI GATC 4 cut(s) 279, 315, 441, 490
BstMCI CGRYCG 2 cut(s) 601, 605
BstMWI GCNNNNNNNGC 6 cut(s) 88, 100, 223, 232, 256, 859
BstNI CCWGG 1 cut(s) 412
BstSCI CCNGG 6 cut(s) 32, 33, 171, 359, 410, 554
BstSLI GKGCMC 1 cut(s) 393
BstV1I GCAGC 4 cut(s) 167, 175, 238, 606
BstV2I GAAGAC 2 cut(s) 24, 518
BstZI CGGCCG 1 cut(s) 602
BsuRI GGCC 5 cut(s) 347, 391, 434, 554, 604
BtgZI GCGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 820
Cac8I GCNNGC 1 cut(s) 523
Cfr10I RCCGGY 2 cut(s) 310, 600
Cfr13I GGNCC 5 cut(s) 132, 389, 390, 545, 840
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 2 cut(s) 125, 724
Csp6I GTAC 4 cut(s) 262, 271, 727, 806
CviAII CATG 1 cut(s) 755
CviQI GTAC 4 cut(s) 262, 271, 727, 806
DdeI CTNAG 1 cut(s) 108
DpnI GATC 4 cut(s) 281, 317, 443, 492
DpnII GATC 4 cut(s) 279, 315, 441, 490
DriI GACNNNNNGTC 1 cut(s) 817
EaeI YGGCCR 3 cut(s) 432, 552, 602
EagI CGGCCG 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 329
Eam1105I GACNNNNNGTC 1 cut(s) 817
EarI CTCTTC 1 cut(s) 329
EciI GGCGGA 1 cut(s) 90
EclXI CGGCCG 1 cut(s) 602
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 3 cut(s) 344, 398, 701
Eco47I GGWCC 3 cut(s) 132, 545, 840
Eco52I CGGCCG 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 3 cut(s) 132, 389, 390
EcoRII CCWGG 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 1 cut(s) 758
FaiI YATR 5 cut(s) 140, 196, 668, 756, 770
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 5 cut(s) 47, 145, 571, 694, 853
FatI CATG 1 cut(s) 754
FauI CCCGC 3 cut(s) 160, 231, 446
FbaI TGATCA 1 cut(s) 315
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 7 cut(s) 92, 95, 156, 189, 204, 227, 595
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 7 cut(s) 92, 95, 156, 189, 204, 227, 595
FspBI CTAG 1 cut(s) 809
GluI GCNGC 7 cut(s) 92, 95, 156, 189, 204, 227, 595
HaeIII GGCC 5 cut(s) 347, 391, 434, 554, 604
HapII CCGG 9 cut(s) 14, 34, 173, 311, 361, 543, 555, 601, 719
HgaI GACGC 2 cut(s) 125, 724
Hin1I GRCGYC 2 cut(s) 117, 716
Hin1II CATG 1 cut(s) 758
HincII GTYRAC 3 cut(s) 573, 609, 781
HindII GTYRAC 3 cut(s) 573, 609, 781
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 4 cut(s) 38, 461, 559, 582
HpaI GTTAAC 1 cut(s) 781
HpaII CCGG 9 cut(s) 14, 34, 173, 311, 361, 543, 555, 601, 719
HphI GGTGA 4 cut(s) 325, 667, 709, 733
Hpy166II GTNNAC 5 cut(s) 169, 424, 573, 609, 781
Hpy188I TCNGA 6 cut(s) 109, 162, 279, 403, 735, 830
Hpy188III TCNNGA 3 cut(s) 445, 627, 787
Hpy8I GTNNAC 5 cut(s) 169, 424, 573, 609, 781
Hpy99I CGWCG 2 cut(s) 577, 718
HpyAV CCTTC 2 cut(s) 313, 740
HpyCH4III ACNGT 3 cut(s) 61, 685, 815
HpyCH4IV ACGT 3 cut(s) 165, 264, 804
HpyCH4V TGCA 3 cut(s) 594, 853, 862
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 100, 223, 232, 256, 859
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 3 cut(s) 165, 264, 804
Hsp92I GRCGYC 2 cut(s) 117, 716
Hsp92II CATG 1 cut(s) 758
KflI GGGWCCC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 315
KspAI GTTAAC 1 cut(s) 781
Kzo9I GATC 4 cut(s) 279, 315, 441, 490
LmnI GCTCC 3 cut(s) 163, 625, 642
Lsp1109I GCAGC 4 cut(s) 167, 175, 238, 606
LweI GCATC 1 cut(s) 808
MaeI CTAG 1 cut(s) 809
MaeII ACGT 3 cut(s) 165, 264, 804
MaeIII GTNAC 1 cut(s) 739
MalI GATC 4 cut(s) 281, 317, 443, 492
MbiI CCGCTC 1 cut(s) 235
MboI GATC 4 cut(s) 279, 315, 441, 490
MboII GAAGA 4 cut(s) 29, 316, 520, 523
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 1 cut(s) 823
MlyI GAGTC 3 cut(s) 47, 470, 553
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 9 cut(s) 57, 88, 103, 106, 108, 332, 389, 403, 495
MroXI GAANNNNTTC 1 cut(s) 515
MseI TTAA 2 cut(s) 471, 780
MspI CCGG 9 cut(s) 14, 34, 173, 311, 361, 543, 555, 601, 719
MspR9I CCNGG 6 cut(s) 34, 35, 173, 361, 412, 556
MvaI CCWGG 1 cut(s) 412
MwoI GCNNNNNNNGC 6 cut(s) 88, 100, 223, 232, 256, 859
NciI CCSGG 5 cut(s) 34, 35, 173, 361, 556
NdeII GATC 4 cut(s) 279, 315, 441, 490
NlaIII CATG 1 cut(s) 758
NlaIV GGNNCC 4 cut(s) 133, 134, 391, 841
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 739
PcsI WCGNNNNNNNCGW 2 cut(s) 437, 710
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 1 cut(s) 582
Pfl23II CGTACG 1 cut(s) 261
PflMI CCANNNNNTGG 2 cut(s) 126, 142
PkrI GCNGC 7 cut(s) 93, 96, 157, 190, 205, 228, 596
PleI GAGTC 3 cut(s) 46, 469, 553
PpsI GAGTC 3 cut(s) 46, 469, 553
Ppu21I YACGTR 1 cut(s) 805
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 4 cut(s) 133, 134, 391, 841
PspOMI GGGCCC 1 cut(s) 389
PspPI GGNCC 5 cut(s) 132, 389, 390, 545, 840
PspPPI RGGWCCY 1 cut(s) 132
RsaI GTAC 4 cut(s) 263, 272, 728, 807
RsaNI GTAC 4 cut(s) 262, 271, 727, 806
SalI GTCGAC 1 cut(s) 571
SaqAI TTAA 2 cut(s) 471, 780
SatI GCNGC 7 cut(s) 92, 95, 156, 189, 204, 227, 595
Sau3AI GATC 4 cut(s) 279, 315, 441, 490
Sau96I GGNCC 5 cut(s) 132, 389, 390, 545, 840
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 3 cut(s) 47, 470, 553
ScrFI CCNGG 6 cut(s) 34, 35, 173, 361, 412, 556
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 1 cut(s) 808
SinI GGWCC 3 cut(s) 132, 545, 840
SmaI CCCGGG 1 cut(s) 35
Sse9I AATT 1 cut(s) 823
SspMI CTAG 1 cut(s) 809
StyD4I CCNGG 6 cut(s) 32, 33, 171, 359, 410, 554
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 3 cut(s) 61, 685, 815
TaiI ACGT 3 cut(s) 168, 267, 807
TaqI TCGA 6 cut(s) 5, 237, 306, 572, 786, 821
TasI AATT 1 cut(s) 823
TatI WGTACW 1 cut(s) 270
TauI GCSGC 3 cut(s) 94, 97, 206
TfiI GAWTC 1 cut(s) 582
Tru1I TTAA 2 cut(s) 471, 780
Tru9I TTAA 2 cut(s) 471, 780
TscAI CASTG 1 cut(s) 820
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 4 cut(s) 155, 188, 226, 594
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 1 cut(s) 861
TspGWI ACGGA 2 cut(s) 612, 719
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 1 cut(s) 820
Van91I CCANNNNNTGG 2 cut(s) 126, 142
VpaK11BI GGWCC 3 cut(s) 132, 545, 840
XapI RAATTY 1 cut(s) 823
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 1 cut(s) 809
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.