FvH4_4g23040

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004816
Physical Location & Seq
Forward (+)
25643429 .. 25645188
1760 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23040.t1

Sequence Viewer

Length: 894 bp
ATGTCAAGCAGACCAGAAGACCAGCTTTCTCCCCGGGAGTCTAATTCCCCCACCACAACCGTCCCCGACATCCACGCCTCCCAACCTCCACCGCCGCCTCCGCCTCCGCCAGCGGAGGAGGACAACAAGAAATATGGGACCCACATAATGGGAGCTCCCGCAGCTCCGAACGTCCACCCGGACAACCAAAAAGCAGCTCTATGGAACGCGGAAGACCACCAGCAAATCCCGCAGCAACCACTCGAAACACAACCGCAACAACCGCCGTACGTTCAGTACTCTCCGATCAACAAGCCGACCAACAACCCCTTCGAGCCGGTGATCAACCTCTTCAATTCTTGGGGGAACAAGGCCGAGACCATCGCCCGGAACATCTGGCACAACCTCAAAACAGGGCCCTCTGTTTCCGAGACCGCCTGGGGAAAACTGAACTTGACGGCCAAGGCTGTAACGGAAGGAGGGTTTGAGGCTCTGTTTAAGCAGATTTTCGCTACTGATCCTAACGAGAAGTTGAAGAAGACTTTCGCTTGCTATCTTTCCACTTCTACCGGACCTGTGGCCGGAACTCTGTATCTGTCCACGGCTAGAGTCGCTTTTTGCAGCGACCGGCCGTTGACCTTTGCTGCTCCGTCTGGACAATCAGCTTGGAGCTACTACAAGGTGATGATACCTTTGACGAATGTTGGGAGAGTGAACCCTGTAGTGATTACGGCAAATCCGACGCCGGAGAAGTACCTTCAGATTGTCACCACGGACGGACATGATTTCTGGTTTATGGGGTTTGTTAATTTCGAGAAAGCTTCCCATCACGTACTGGAAAGTGTGTCGAATTTGAGAGCAGCTGGGGCTGCTGGGACCAATGAAGTGCAACCAGCGCAACATCCTGTCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

298

Amino Acids

32.47

Weight (kDa)

6.3

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 158 - 271 2.5e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccII CGCG 1 cut(s) 209
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 3 cut(s) 438, 558, 608
AcsI RAATTY 1 cut(s) 829
AcuI CTGAAG 1 cut(s) 722
AcyI GRCGYC 1 cut(s) 722
AfaI GTAC 4 cut(s) 269, 278, 734, 813
AfiI CCNNNNNNNGG 3 cut(s) 148, 366, 560
AgsI TTSAA 2 cut(s) 334, 514
AjnI CCWGG 1 cut(s) 416
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 8 cut(s) 25, 155, 164, 197, 644, 651, 800, 842
AluI AGCT 8 cut(s) 25, 155, 164, 197, 644, 651, 800, 842
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 350, 404
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 5 cut(s) 351, 395, 438, 558, 608
ApaI GGGCCC 1 cut(s) 399
ApeKI GCWGC 7 cut(s) 161, 194, 232, 600, 623, 839, 848
ApoI RAATTY 1 cut(s) 829
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 1 cut(s) 877
AspS9I GGNCC 5 cut(s) 138, 395, 396, 551, 855
AsuC2I CCSGG 4 cut(s) 34, 35, 179, 367
AsuHPI GGTGA 3 cut(s) 331, 673, 739
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 3 cut(s) 138, 551, 855
BaeGI GKGCMC 1 cut(s) 399
BanII GRGCYC 2 cut(s) 157, 399
BbsI GAAGAC 3 cut(s) 24, 219, 524
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 7 cut(s) 173, 206, 244, 610, 612, 835, 851
BccI CCATC 2 cut(s) 368, 813
BceAI ACGGC 5 cut(s) 250, 453, 595, 597, 726
BciT130I CCWGG 1 cut(s) 418
BclI TGATCA 1 cut(s) 321
BcnI CCSGG 4 cut(s) 34, 35, 179, 367
BcoDI GTCTC 2 cut(s) 350, 404
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 699
BisI GCNGC 8 cut(s) 95, 162, 195, 233, 601, 624, 840, 849
BlsI GCNGC 8 cut(s) 96, 163, 196, 234, 602, 625, 841, 850
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 5 cut(s) 34, 35, 179, 367, 418
Bme18I GGWCC 3 cut(s) 138, 551, 855
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 138, 395, 396, 551, 855
BmiI GGNNCC 4 cut(s) 139, 140, 397, 856
BmrFI CCNGG 5 cut(s) 34, 35, 179, 367, 418
BpiI GAAGAC 3 cut(s) 24, 219, 524
BpuMI CCSGG 4 cut(s) 34, 35, 179, 367
BsaAI YACGTR 1 cut(s) 811
BsaHI GRCGYC 1 cut(s) 722
BsaI GGTCTC 2 cut(s) 350, 404
BsaJI CCNNGG 6 cut(s) 32, 33, 417, 441, 579, 750
BsaWI WCCGGW 1 cut(s) 548
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 366, 560
Bse118I RCCGGY 2 cut(s) 316, 606
Bse1I ACTGG 1 cut(s) 819
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 6 cut(s) 32, 33, 417, 441, 579, 750
BseGI GGATG 2 cut(s) 69, 880
BseLI CCNNNNNNNGG 3 cut(s) 148, 366, 560
BseNI ACTGG 1 cut(s) 819
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 399
BseX3I CGGCCG 1 cut(s) 608
BseXI GCAGC 7 cut(s) 173, 206, 244, 610, 612, 835, 851
BseYI CCCAGC 2 cut(s) 842, 851
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 2 cut(s) 607, 611
BshFI GGCC 5 cut(s) 353, 397, 440, 560, 610
BsiEI CGRYCG 2 cut(s) 607, 611
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 8 cut(s) 34, 179, 317, 367, 549, 561, 607, 725
BsiWI CGTACG 1 cut(s) 267
BslFI GGGAC 3 cut(s) 47, 151, 868
BslI CCNNNNNNNGG 3 cut(s) 148, 366, 560
BsmAI GTCTC 2 cut(s) 350, 404
BsmFI GGGAC 3 cut(s) 47, 151, 868
BsnI GGCC 5 cut(s) 353, 397, 440, 560, 610
Bso31I GGTCTC 2 cut(s) 350, 404
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 157, 399
Bsp143I GATC 3 cut(s) 285, 321, 496
BspANI GGCC 5 cut(s) 353, 397, 440, 560, 610
BspFNI CGCG 1 cut(s) 209
BspLI GGNNCC 4 cut(s) 139, 140, 397, 856
BspPI GGATC 1 cut(s) 491
BspTNI GGTCTC 2 cut(s) 350, 404
BsrFI RCCGGY 2 cut(s) 316, 606
BsrI ACTGG 1 cut(s) 819
BssAI RCCGGY 2 cut(s) 316, 606
BssECI CCNNGG 6 cut(s) 32, 33, 417, 441, 579, 750
BssMI GATC 3 cut(s) 285, 321, 496
BssNI GRCGYC 1 cut(s) 722
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 1 cut(s) 722
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 2 cut(s) 111, 529
BstDSI CCRYGG 2 cut(s) 579, 750
BstF5I GGATG 2 cut(s) 69, 880
BstFNI CGCG 1 cut(s) 209
BstHHI GCGC 1 cut(s) 877
BstKTI GATC 3 cut(s) 288, 324, 499
BstMAI GTCTC 2 cut(s) 350, 404
BstMBI GATC 3 cut(s) 285, 321, 496
BstMCI CGRYCG 2 cut(s) 607, 611
BstMWI GCNNNNNNNGC 8 cut(s) 100, 161, 229, 262, 590, 845, 848, 874
BstNI CCWGG 1 cut(s) 418
BstSCI CCNGG 5 cut(s) 32, 33, 177, 365, 416
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 1 cut(s) 399
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 7 cut(s) 173, 206, 244, 610, 612, 835, 851
BstV2I GAAGAC 3 cut(s) 24, 219, 524
BstZI CGGCCG 1 cut(s) 608
BsuRI GGCC 5 cut(s) 353, 397, 440, 560, 610
BtgI CCRYGG 2 cut(s) 579, 750
BtgZI GCGATG 1 cut(s) 346
BtsCI GGATG 2 cut(s) 69, 880
Cac8I GCNNGC 2 cut(s) 111, 529
CfoI GCGC 1 cut(s) 877
Cfr10I RCCGGY 2 cut(s) 316, 606
Cfr13I GGNCC 5 cut(s) 138, 395, 396, 551, 855
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 1 cut(s) 730
Csp6I GTAC 4 cut(s) 268, 277, 733, 812
CviAII CATG 1 cut(s) 761
CviQI GTAC 4 cut(s) 268, 277, 733, 812
DpnI GATC 3 cut(s) 287, 323, 498
DpnII GATC 3 cut(s) 285, 321, 496
EaeI YGGCCR 3 cut(s) 438, 558, 608
EagI CGGCCG 1 cut(s) 608
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
EciI GGCGGA 2 cut(s) 90, 96
Ecl136II GAGCTC 1 cut(s) 155
EclXI CGGCCG 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 441
Eco24I GRGCYC 2 cut(s) 157, 399
Eco31I GGTCTC 2 cut(s) 350, 404
Eco47I GGWCC 3 cut(s) 138, 551, 855
Eco52I CGGCCG 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 722
Eco88I CYCGRG 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 155
EcoO109I RGGNCCY 3 cut(s) 138, 395, 396
EcoRII CCWGG 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 157, 399
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 1 cut(s) 764
FaiI YATR 5 cut(s) 135, 146, 202, 762, 776
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 3 cut(s) 47, 151, 868
FatI CATG 1 cut(s) 760
FauI CCCGC 2 cut(s) 166, 237
FbaI TGATCA 1 cut(s) 321
Fnu4HI GCNGC 8 cut(s) 95, 162, 195, 233, 601, 624, 840, 849
FokI GGATG 2 cut(s) 56, 867
FriOI GRGCYC 2 cut(s) 157, 399
Fsp4HI GCNGC 8 cut(s) 95, 162, 195, 233, 601, 624, 840, 849
FspBI CTAG 1 cut(s) 585
GlaI GCGC 1 cut(s) 876
GluI GCNGC 8 cut(s) 95, 162, 195, 233, 601, 624, 840, 849
GsaI CCCAGC 2 cut(s) 846, 855
HaeIII GGCC 5 cut(s) 353, 397, 440, 560, 610
HapII CCGG 8 cut(s) 34, 179, 317, 367, 549, 561, 607, 725
HgaI GACGC 1 cut(s) 730
HhaI GCGC 1 cut(s) 877
Hin1I GRCGYC 1 cut(s) 722
Hin1II CATG 1 cut(s) 764
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 2 cut(s) 38, 588
HpaII CCGG 8 cut(s) 34, 179, 317, 367, 549, 561, 607, 725
HphI GGTGA 3 cut(s) 331, 673, 739
Hpy166II GTNNAC 4 cut(s) 175, 579, 615, 694
Hpy188I TCNGA 5 cut(s) 168, 285, 409, 720, 741
Hpy188III TCNNGA 2 cut(s) 633, 793
Hpy8I GTNNAC 4 cut(s) 175, 579, 615, 694
Hpy99I CGWCG 1 cut(s) 724
HpyAV CCTTC 3 cut(s) 319, 449, 746
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 3 cut(s) 171, 270, 810
HpyCH4V TGCA 2 cut(s) 600, 868
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 161, 229, 262, 590, 845, 848, 874
HpySE526I ACGT 3 cut(s) 171, 270, 810
Hsp92I GRCGYC 1 cut(s) 722
Hsp92II CATG 1 cut(s) 764
HspAI GCGC 1 cut(s) 875
KflI GGGWCCC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 321
Kzo9I GATC 3 cut(s) 285, 321, 496
LmnI GCTCC 5 cut(s) 152, 160, 169, 631, 648
Lsp1109I GCAGC 7 cut(s) 173, 206, 244, 610, 612, 835, 851
MaeI CTAG 1 cut(s) 585
MaeII ACGT 3 cut(s) 171, 270, 810
MaeIII GTNAC 2 cut(s) 448, 745
MalI GATC 3 cut(s) 287, 323, 498
MboI GATC 3 cut(s) 285, 321, 496
MboII GAAGA 5 cut(s) 29, 224, 322, 526, 529
MhlI GDGCHC 2 cut(s) 157, 399
MluCI AATT 4 cut(s) 43, 334, 787, 829
MlyI GAGTC 2 cut(s) 47, 597
MmeI TCCRAC 1 cut(s) 743
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 2 cut(s) 477, 786
MspA1I CMGCKG 2 cut(s) 113, 842
MspI CCGG 8 cut(s) 34, 179, 317, 367, 549, 561, 607, 725
MspR9I CCNGG 5 cut(s) 34, 35, 179, 367, 418
MvaI CCWGG 1 cut(s) 418
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 8 cut(s) 100, 161, 229, 262, 590, 845, 848, 874
NciI CCSGG 4 cut(s) 34, 35, 179, 367
NdeII GATC 3 cut(s) 285, 321, 496
NlaIII CATG 1 cut(s) 764
NlaIV GGNNCC 4 cut(s) 139, 140, 397, 856
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 716
PdmI GAANNNNTTC 1 cut(s) 521
Pfl23II CGTACG 1 cut(s) 267
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 8 cut(s) 96, 163, 196, 234, 602, 625, 841, 850
PleI GAGTC 2 cut(s) 46, 596
PpsI GAGTC 2 cut(s) 46, 596
Ppu21I YACGTR 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 416
PspFI CCCAGC 2 cut(s) 842, 851
PspGI CCWGG 1 cut(s) 416
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 4 cut(s) 139, 140, 397, 856
PspOMI GGGCCC 1 cut(s) 395
PspPI GGNCC 5 cut(s) 138, 395, 396, 551, 855
PspPPI RGGWCCY 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 4 cut(s) 269, 278, 734, 813
RsaNI GTAC 4 cut(s) 268, 277, 733, 812
SacI GAGCTC 1 cut(s) 157
SaqAI TTAA 2 cut(s) 477, 786
SatI GCNGC 8 cut(s) 95, 162, 195, 233, 601, 624, 840, 849
Sau3AI GATC 3 cut(s) 285, 321, 496
Sau96I GGNCC 5 cut(s) 138, 395, 396, 551, 855
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 2 cut(s) 47, 597
ScrFI CCNGG 5 cut(s) 34, 35, 179, 367, 418
SduI GDGCHC 2 cut(s) 157, 399
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 3 cut(s) 138, 551, 855
SmaI CCCGGG 1 cut(s) 35
Sse9I AATT 4 cut(s) 43, 334, 787, 829
SspMI CTAG 1 cut(s) 585
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 5 cut(s) 32, 33, 177, 365, 416
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 3 cut(s) 174, 273, 813
TaqI TCGA 4 cut(s) 243, 312, 792, 827
TasI AATT 4 cut(s) 43, 334, 787, 829
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 97
Tru1I TTAA 2 cut(s) 477, 786
Tru9I TTAA 2 cut(s) 477, 786
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 7 cut(s) 161, 194, 232, 600, 623, 839, 848
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 1 cut(s) 876
TspGWI ACGGA 4 cut(s) 467, 618, 767, 771
TspMI CCCGGG 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 3 cut(s) 138, 551, 855
XapI RAATTY 1 cut(s) 829
XmaI CCCGGG 1 cut(s) 33
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 1 cut(s) 585
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.