MD17G1009200.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004816
Physical Location & Seq
Forward (+)
584507 .. 586602
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1009200.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGAACCACCTCAATAACGAAGCATCCCAACCTCCCCTCTACCCCTCCGTTTCTCCTCCCACACACCAAAACACAAACCCTTTTCTCCCAACACCTCCCGCTGAAGACATCAACCCTTTTCATCAAACACCTCCGTCAATGCCGCCATCCTCTTCCGAAGAAACACCTCCTCACGATGCATCCTCACCGCAGAATGAGAAAACCCAAGAACCAAAACAAGCTACTCATGATCAAGATATTCCATCCTCATCAAGCGGCTCATCTCCTCCGTCAATCCCACCAACTCCAAGTGAGGAAGAAAAATCAGCGCAGTGGGGGACTCACGTCATGGGGCACCCTGCCGTCCCAACTAGCCATCCGGACAACAAAAAAGCAGCTCTGTGGGGTGCTGCCGGAACCGAGCAAGCCCAGAATCCCTACCTCCAGTACAAACCCCTTGACAAGTCCAACAACACCAGTCCAATGGAATCCATGCTCCACGTCTTCAACTCCTGGACCAACAAGGCCGAGTCCATGGCCAACAACATCTGGCACAACTTGAAAACGGGGACATCGGTGTCTGGAGCTGCGTGGGCGAAGATGAACCTGACTGCGAAAGCAATAACAGGAGGAGGATTTGAGGCTCTGTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTGCATAAGTCATTTGCCAGTTATCTCTCCACATCCACAGGACCAGTTGCCGGAACTCTTTACCTCTCCAATTTCCATACAGCTTTCTGCAGCGACCGCCCTTTGTCCTTCACCGCCCCCTCCGGCCAGGTCACCTGGAGCTACTACAAAATAATGGTACCATTGGGAAATATTGCAACTATCAATCCAGTGGTGATGAGGGAAAATCCAAGGGAAAAGTACATCCAGATTGTGACGATCGACGGCCATGATTTCTGGTTCATGGGTTTTGTTAATTACGAAAAGGCGTCGAGGCATTTGACCCAAAGTATTTCAACCTTTGTGGCATCTGGAGTTGCCGTCAAACCAGCCGTTGCTGCTGACAATGACATTGCATACCAGAAGCCTGACAAGGCTGACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

356

Amino Acids

38.77

Weight (kDa)

6.14

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 211 - 322 6e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 556
Acc65I GGTACC 1 cut(s) 820
AccB1I GGYRCC 2 cut(s) 333, 820
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 6 cut(s) 99, 143, 188, 255, 760, 777
AcoI YGGCCR 3 cut(s) 516, 787, 907
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 950
AfaI GTAC 4 cut(s) 428, 629, 822, 884
AfiI CCNNNNNNNGG 1 cut(s) 713
AgsI TTSAA 3 cut(s) 487, 541, 978
AjiI CACGTC 2 cut(s) 325, 481
AjnI CCWGG 3 cut(s) 491, 789, 797
AluBI AGCT 6 cut(s) 221, 377, 566, 664, 746, 804
AluI AGCT 6 cut(s) 221, 377, 566, 664, 746, 804
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 4 cut(s) 504, 516, 787, 907
ApeKI GCWGC 6 cut(s) 374, 389, 566, 664, 753, 1019
Asp718I GGTACC 1 cut(s) 820
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 2 cut(s) 495, 704
AsuHPI GGTGA 4 cut(s) 177, 766, 787, 868
AvaII GGWCC 2 cut(s) 495, 704
BaeGI GKGCMC 1 cut(s) 336
BalI TGGCCA 1 cut(s) 518
BanI GGYRCC 2 cut(s) 333, 820
BbsI GAAGAC 2 cut(s) 111, 475
BbvI GCAGC 6 cut(s) 376, 386, 553, 651, 765, 1006
BccI CCATC 3 cut(s) 154, 250, 363
BceAI ACGGC 4 cut(s) 326, 922, 986, 998
BciT130I CCWGG 3 cut(s) 493, 791, 799
BclI TGATCA 1 cut(s) 229
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 751
BisI GCNGC 8 cut(s) 143, 256, 375, 390, 567, 665, 754, 1020
BlsI GCNGC 8 cut(s) 144, 257, 376, 391, 568, 666, 755, 1021
Bme1390I CCNGG 3 cut(s) 493, 791, 799
Bme18I GGWCC 2 cut(s) 495, 704
BmgBI CACGTC 2 cut(s) 325, 481
BmgT120I GGNCC 2 cut(s) 495, 704
BmiI GGNNCC 3 cut(s) 335, 397, 822
BmrFI CCNGG 3 cut(s) 493, 791, 799
BmsI GCATC 4 cut(s) 32, 166, 188, 998
BoxI GACNNNNGTC 1 cut(s) 323
BpiI GAAGAC 2 cut(s) 111, 475
BpmI CTGGAG 4 cut(s) 407, 582, 820, 1014
BsaHI GRCGYC 1 cut(s) 950
BsaJI CCNNGG 2 cut(s) 513, 872
BsaWI WCCGGW 1 cut(s) 358
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 1 cut(s) 713
Bse1I ACTGG 5 cut(s) 424, 456, 681, 707, 851
Bse3DI GCAATG 1 cut(s) 1032
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 3 cut(s) 493, 791, 799
BseDI CCNNGG 2 cut(s) 513, 872
BseGI GGATG 7 cut(s) 23, 146, 179, 242, 355, 695, 885
BseLI CCNNNNNNNGG 1 cut(s) 713
BseMI GCAATG 1 cut(s) 1032
BseNI ACTGG 5 cut(s) 424, 456, 681, 707, 851
BseRI GAGGAG 4 cut(s) 45, 159, 255, 624
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 6 cut(s) 376, 386, 553, 651, 765, 1006
Bsh1285I CGRYCG 2 cut(s) 760, 903
BshFI GGCC 4 cut(s) 506, 518, 789, 909
BshNI GGYRCC 2 cut(s) 333, 820
BsiEI CGRYCG 2 cut(s) 760, 903
BsiSI CCGG 4 cut(s) 359, 393, 714, 786
BslFI GGGAC 3 cut(s) 329, 331, 562
BslI CCNNNNNNNGG 1 cut(s) 713
BsmFI GGGAC 3 cut(s) 329, 331, 562
BsnI GGCC 4 cut(s) 506, 518, 789, 909
Bsp1286I GDGCHC 1 cut(s) 336
Bsp13I TCCGGA 1 cut(s) 358
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 2 cut(s) 229, 900
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 6 cut(s) 99, 143, 188, 255, 760, 777
BspANI GGCC 4 cut(s) 506, 518, 789, 909
BspEI TCCGGA 1 cut(s) 358
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 3 cut(s) 335, 397, 822
BspMAI CTGCAG 1 cut(s) 755
BspT107I GGYRCC 2 cut(s) 333, 820
BsrDI GCAATG 1 cut(s) 1032
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 5 cut(s) 424, 456, 681, 707, 851
BssECI CCNNGG 2 cut(s) 513, 872
BssMI GATC 2 cut(s) 229, 900
BssNI GRCGYC 1 cut(s) 950
BssT1I CCWWGG 2 cut(s) 513, 872
Bst2UI CCWGG 3 cut(s) 493, 791, 799
Bst6I CTCTTC 1 cut(s) 157
BstACI GRCGYC 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 513
BstEII GGTNACC 1 cut(s) 793
BstF5I GGATG 7 cut(s) 23, 146, 179, 242, 355, 695, 885
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 2 cut(s) 232, 903
BstMBI GATC 2 cut(s) 229, 900
BstMCI CGRYCG 2 cut(s) 760, 903
BstMWI GCNNNNNNNGC 3 cut(s) 572, 759, 1019
BstNI CCWGG 3 cut(s) 493, 791, 799
BstPAI GACNNNNGTC 1 cut(s) 323
BstPI GGTNACC 1 cut(s) 793
BstSCI CCNGG 3 cut(s) 491, 789, 797
BstSFI CTRYAG 1 cut(s) 751
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 6 cut(s) 376, 386, 553, 651, 765, 1006
BstV2I GAAGAC 2 cut(s) 111, 475
BstXI CCANNNNNNTGG 1 cut(s) 463
BsuRI GGCC 4 cut(s) 506, 518, 789, 909
BtgI CCRYGG 1 cut(s) 513
BtrI CACGTC 2 cut(s) 325, 481
BtsCI GGATG 7 cut(s) 23, 146, 179, 242, 355, 695, 885
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 2 cut(s) 317, 858
Cac8I GCNNGC 1 cut(s) 405
CciI TCATGA 1 cut(s) 226
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 495, 704
CseI GACGC 1 cut(s) 939
Csp6I GTAC 4 cut(s) 427, 628, 821, 883
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 6 cut(s) 227, 328, 472, 514, 911, 925
CviQI GTAC 4 cut(s) 427, 628, 821, 883
DpnI GATC 2 cut(s) 231, 902
DpnII GATC 2 cut(s) 229, 900
DrdI GACNNNNNNGTC 1 cut(s) 556
DseDI GACNNNNNNGTC 1 cut(s) 556
EaeI YGGCCR 3 cut(s) 516, 787, 907
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 513, 872
Eco47I GGWCC 2 cut(s) 495, 704
Eco57I CTGAAG 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 793
EcoO65I GGTNACC 1 cut(s) 793
EcoRII CCWGG 3 cut(s) 491, 789, 797
EcoT14I CCWWGG 2 cut(s) 513, 872
EcoT22I ATGCAT 1 cut(s) 181
ErhI CCWWGG 2 cut(s) 513, 872
FaeI CATG 6 cut(s) 230, 331, 475, 517, 914, 928
FaiI YATR 9 cut(s) 228, 329, 473, 515, 669, 741, 912, 926, 1039
FaqI GGGAC 3 cut(s) 329, 331, 562
FatI CATG 6 cut(s) 226, 327, 471, 513, 910, 924
FauI CCCGC 1 cut(s) 106
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 8 cut(s) 143, 256, 375, 390, 567, 665, 754, 1020
FokI GGATG 7 cut(s) 10, 133, 166, 229, 342, 682, 872
Fsp4HI GCNGC 8 cut(s) 143, 256, 375, 390, 567, 665, 754, 1020
FspBI CTAG 1 cut(s) 351
GlaI GCGC 1 cut(s) 309
GluI GCNGC 8 cut(s) 143, 256, 375, 390, 567, 665, 754, 1020
GsuI CTGGAG 4 cut(s) 407, 582, 820, 1014
HaeIII GGCC 4 cut(s) 506, 518, 789, 909
HapII CCGG 4 cut(s) 359, 393, 714, 786
HgaI GACGC 1 cut(s) 939
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 950
Hin1II CATG 6 cut(s) 230, 331, 475, 517, 914, 928
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HinfI GANTC 4 cut(s) 319, 412, 467, 509
HpaII CCGG 4 cut(s) 359, 393, 714, 786
HphI GGTGA 4 cut(s) 177, 766, 787, 868
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 7 cut(s) 173, 227, 233, 359, 561, 889, 993
Hpy99I CGWCG 2 cut(s) 908, 955
HpyAV CCTTC 1 cut(s) 781
HpyCH4IV ACGT 2 cut(s) 324, 480
HpyCH4V TGCA 5 cut(s) 179, 667, 753, 839, 1037
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 759, 1019
HpySE526I ACGT 2 cut(s) 324, 480
Hsp92I GRCGYC 1 cut(s) 950
Hsp92II CATG 6 cut(s) 230, 331, 475, 517, 914, 928
HspAI GCGC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 358
KpnI GGTACC 1 cut(s) 824
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 229, 900
LmnI GCTCC 3 cut(s) 480, 563, 801
Lsp1109I GCAGC 6 cut(s) 376, 386, 553, 651, 765, 1006
LweI GCATC 4 cut(s) 32, 166, 188, 998
MaeI CTAG 1 cut(s) 351
MaeII ACGT 2 cut(s) 324, 480
MaeIII GTNAC 2 cut(s) 793, 895
MalI GATC 2 cut(s) 231, 902
MboI GATC 2 cut(s) 229, 900
MboII GAAGA 6 cut(s) 116, 144, 170, 308, 475, 589
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 2 cut(s) 733, 937
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 2 cut(s) 313, 518
MmeI TCCRAC 1 cut(s) 471
Mox20I TGGCCA 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 181
MroI TCCGGA 1 cut(s) 358
MscI TGGCCA 1 cut(s) 518
MseI TTAA 1 cut(s) 936
Msp20I TGGCCA 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 4 cut(s) 359, 393, 714, 786
MspR9I CCNGG 3 cut(s) 493, 791, 799
MvaI CCWGG 3 cut(s) 493, 791, 799
MwoI GCNNNNNNNGC 3 cut(s) 572, 759, 1019
NcoI CCATGG 1 cut(s) 513
NdeII GATC 2 cut(s) 229, 900
NlaIII CATG 6 cut(s) 230, 331, 475, 517, 914, 928
NlaIV GGNNCC 3 cut(s) 335, 397, 822
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 2 cut(s) 793, 895
NsiI ATGCAT 1 cut(s) 181
PagI TCATGA 1 cut(s) 226
PfeI GAWTC 2 cut(s) 412, 467
PfoI TCCNGGA 1 cut(s) 491
PkrI GCNGC 8 cut(s) 144, 257, 376, 391, 568, 666, 755, 1021
Ple19I CGATCG 1 cut(s) 903
PleI GAGTC 2 cut(s) 313, 517
PpsI GAGTC 2 cut(s) 313, 517
PshAI GACNNNNGTC 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 491, 789, 797
PspEI GGTNACC 1 cut(s) 793
PspGI CCWGG 3 cut(s) 491, 789, 797
PspN4I GGNNCC 3 cut(s) 335, 397, 822
PspPI GGNCC 2 cut(s) 495, 704
PstI CTGCAG 1 cut(s) 755
PvuI CGATCG 1 cut(s) 903
RsaI GTAC 4 cut(s) 428, 629, 822, 884
RsaNI GTAC 4 cut(s) 427, 628, 821, 883
SaqAI TTAA 1 cut(s) 936
SatI GCNGC 8 cut(s) 143, 256, 375, 390, 567, 665, 754, 1020
Sau3AI GATC 2 cut(s) 229, 900
Sau96I GGNCC 2 cut(s) 495, 704
SchI GAGTC 2 cut(s) 313, 518
ScrFI CCNGG 3 cut(s) 493, 791, 799
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 4 cut(s) 32, 166, 188, 998
SfcI CTRYAG 1 cut(s) 751
SinI GGWCC 2 cut(s) 495, 704
Sse9I AATT 2 cut(s) 733, 937
SsiI CCGC 6 cut(s) 99, 143, 188, 255, 760, 777
SspI AATATT 1 cut(s) 835
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 491, 789, 797
StyI CCWWGG 2 cut(s) 513, 872
TaiI ACGT 2 cut(s) 327, 483
TaqI TCGA 2 cut(s) 903, 953
TasI AATT 2 cut(s) 733, 937
TatI WGTACW 3 cut(s) 426, 627, 882
TauI GCSGC 2 cut(s) 145, 258
TfiI GAWTC 2 cut(s) 412, 467
Tru1I TTAA 1 cut(s) 936
Tru9I TTAA 1 cut(s) 936
TscAI CASTG 2 cut(s) 317, 858
TseFI GTSAC 2 cut(s) 793, 895
TseI GCWGC 6 cut(s) 374, 389, 566, 664, 753, 1019
Tsp45I GTSAC 2 cut(s) 793, 895
TspDTI ATGAA 4 cut(s) 17, 110, 596, 913
TspGWI ACGGA 3 cut(s) 37, 123, 258
TspRI CASTG 2 cut(s) 317, 858
VpaK11BI GGWCC 2 cut(s) 495, 704
XspI CTAG 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.