MD09G1005200.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004816
Physical Location & Seq
Forward (+)
402082 .. 404041
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1005200.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGAACCACCCCAACAACCAAGCATCCCAACCTCCTCTATACCCCTCCATTTCTCCTCCCTCTCACCAAGACATAAACCCCTTCCTCCCAACTCCTCCGCCTGAAGATATCAACCCTTTTCATCAAACACCTGCGCCGATGCCACCGTCCTCGTCGCCGCTAATCCATTTCGAAACACCTCCTAGCGTATCCTCACCTCAGAAAGGGAAAACCGAAGAGCTAAAACACACTACTCCTGATCAAGATGTTCCGTCGTCGTCAAGCGGCGCATCTCCTCCGCCAATCCCACCAACTCCAAGTGAGGAAGAAAAATCAGCGCAGTGGGGGACTCACGTCATGGGGCACCCTGCCGTCCCAACATGCCATCCGGACAACAAAAAAGCAGCTCTGTGGGGTGCTGCCGGAACCGACCAAGCCCATAATCCCTACCTCCAGTACAAACCCCTTGACAAGTCCAACAACACCAGTCCATTGGAATCCATGCTCCACGTCTTCAACTCCTGGAGCAACAAGGCCGAGTCCATGGCCAACAACATCTGGCACAACCTGAAAACGGGGACATCGGTGTCTGGAGCAGCGTGGGCGAAGATGAACGTGACGGCGAAAGCAATAACAGGAGGAGGATTTGAGGCTCTGTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTGTACAAGTCATTTGCCTGTTATCTGTCCACGTCCACAGGACCAGTTGCCGGAACTCTTTACCTCTCTAATATCCATACAGCTTTCTGCAGCGACCGCCCTTTATCCTTCACCGCCCCCTCCGGCCAGGTCACCTGGAGCTACTACAAAATAATGGTACCATTGGGAAATATTGCAACTATCAATCCAGTGGTGATGAGGGAAAATCCAACGGAGAAGTACATCCAGATTGTGACGATCGACGGCCATGATTTCTGGTTCATGGGTTTTGTTAATTACGAAAAGGCGTCGAGGCATTTGAGCCAAAGTATTTCAACATTTGTGGCATCTGGAGTTGCCGTCGAACCAGTCGTTGCTGCAGACAAGGACATTGCATACCAGAAGCCTGACAAGGCTGACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

359

Amino Acids

39.0

Weight (kDa)

6.26

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 214 - 322 1.9e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 139
Acc65I GGTACC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 342, 829
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 6 cut(s) 98, 158, 264, 278, 769, 786
AcoI YGGCCR 3 cut(s) 525, 796, 916
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 959
AfaI GTAC 5 cut(s) 437, 638, 677, 831, 893
AfiI CCNNNNNNNGG 3 cut(s) 203, 553, 722
AgsI TTSAA 2 cut(s) 496, 987
AjiI CACGTC 3 cut(s) 334, 490, 705
AjnI CCWGG 3 cut(s) 500, 798, 806
AluBI AGCT 5 cut(s) 220, 386, 673, 755, 813
AluI AGCT 5 cut(s) 220, 386, 673, 755, 813
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 4 cut(s) 513, 525, 796, 916
ApeKI GCWGC 5 cut(s) 383, 398, 575, 762, 1028
Asp718I GGTACC 1 cut(s) 829
AspLEI GCGC 3 cut(s) 136, 269, 319
AspS9I GGNCC 1 cut(s) 713
AsuHPI GGTGA 5 cut(s) 56, 186, 775, 796, 877
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 2 cut(s) 342, 829
BbsI GAAGAC 1 cut(s) 484
BbvI GCAGC 5 cut(s) 385, 395, 587, 774, 1015
BccI CCATC 1 cut(s) 372
BceAI ACGGC 4 cut(s) 335, 615, 931, 995
BciT130I CCWGG 3 cut(s) 502, 800, 808
BciVI GTATCC 1 cut(s) 199
BclI TGATCA 1 cut(s) 238
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 2 cut(s) 760, 1029
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 199
BisI GCNGC 7 cut(s) 158, 265, 384, 399, 576, 763, 1029
BlsI GCNGC 7 cut(s) 159, 266, 385, 400, 577, 764, 1030
Bme1390I CCNGG 3 cut(s) 502, 800, 808
Bme18I GGWCC 1 cut(s) 713
BmgBI CACGTC 3 cut(s) 334, 490, 705
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 3 cut(s) 344, 406, 831
BmrFI CCNGG 3 cut(s) 502, 800, 808
BmsI GCATC 4 cut(s) 32, 129, 278, 1007
BoxI GACNNNNGTC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 484
BpmI CTGGAG 5 cut(s) 416, 523, 591, 829, 1023
Bpu14I TTCGAA 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 959
BsaJI CCNNGG 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 996, 1026
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 553, 722
Bse1I ACTGG 5 cut(s) 433, 465, 716, 860, 1019
Bse3DI GCAATG 1 cut(s) 1041
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 3 cut(s) 502, 800, 808
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 3 cut(s) 23, 364, 894
BseLI CCNNNNNNNGG 3 cut(s) 203, 553, 722
BseMI GCAATG 1 cut(s) 1041
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 5 cut(s) 433, 465, 716, 860, 1019
BseRI GAGGAG 5 cut(s) 24, 45, 84, 264, 633
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 5 cut(s) 385, 395, 587, 774, 1015
Bsh1285I CGRYCG 2 cut(s) 769, 912
BshFI GGCC 4 cut(s) 515, 527, 798, 918
BshNI GGYRCC 2 cut(s) 342, 829
BsiEI CGRYCG 2 cut(s) 769, 912
BsiSI CCGG 4 cut(s) 368, 402, 723, 795
BslFI GGGAC 3 cut(s) 338, 340, 571
BslI CCNNNNNNNGG 3 cut(s) 203, 553, 722
BsmFI GGGAC 3 cut(s) 338, 340, 571
BsnI GGCC 4 cut(s) 515, 527, 798, 918
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 345
Bsp13I TCCGGA 1 cut(s) 367
Bsp1407I TGTACA 2 cut(s) 636, 675
Bsp143I GATC 2 cut(s) 238, 909
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 6 cut(s) 98, 158, 264, 278, 769, 786
BspANI GGCC 4 cut(s) 515, 527, 798, 918
BspCNI CTCAG 1 cut(s) 211
BspEI TCCGGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 344, 406, 831
BspMAI CTGCAG 2 cut(s) 764, 1033
BspMI ACCTGC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 342, 829
BsrDI GCAATG 1 cut(s) 1041
BsrGI TGTACA 2 cut(s) 636, 675
BsrI ACTGG 5 cut(s) 433, 465, 716, 860, 1019
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 2 cut(s) 238, 909
BssNI GRCGYC 1 cut(s) 959
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 502, 800, 808
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 959
BstAUI TGTACA 2 cut(s) 636, 675
BstBI TTCGAA 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 522
BstEII GGTNACC 1 cut(s) 802
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 23, 364, 894
BstHHI GCGC 3 cut(s) 136, 269, 319
BstKTI GATC 2 cut(s) 241, 912
BstMBI GATC 2 cut(s) 238, 909
BstMCI CGRYCG 2 cut(s) 769, 912
BstMWI GCNNNNNNNGC 2 cut(s) 581, 768
BstNI CCWGG 3 cut(s) 502, 800, 808
BstNSI RCATGY 1 cut(s) 363
BstPAI GACNNNNGTC 1 cut(s) 332
BstPI GGTNACC 1 cut(s) 802
BstSCI CCNGG 3 cut(s) 500, 798, 806
BstSFI CTRYAG 2 cut(s) 760, 1029
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 5 cut(s) 385, 395, 587, 774, 1015
BstV2I GAAGAC 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 4 cut(s) 515, 527, 798, 918
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 3 cut(s) 334, 490, 705
BtsCI GGATG 3 cut(s) 23, 364, 894
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 2 cut(s) 326, 867
BveI ACCTGC 1 cut(s) 139
CfoI GCGC 3 cut(s) 136, 269, 319
Cfr13I GGNCC 1 cut(s) 713
CseI GACGC 1 cut(s) 948
Csp6I GTAC 5 cut(s) 436, 637, 676, 830, 892
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 6 cut(s) 337, 360, 481, 523, 920, 934
CviQI GTAC 5 cut(s) 436, 637, 676, 830, 892
DdeI CTNAG 1 cut(s) 198
DpnI GATC 2 cut(s) 240, 911
DpnII GATC 2 cut(s) 238, 909
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 3 cut(s) 525, 796, 916
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 2 cut(s) 87, 267
Eco130I CCWWGG 1 cut(s) 522
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 713
Eco57I CTGAAG 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 802
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 802
EcoRII CCWGG 3 cut(s) 500, 798, 806
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 6 cut(s) 340, 363, 484, 526, 923, 937
FaqI GGGAC 3 cut(s) 338, 340, 571
FatI CATG 6 cut(s) 336, 359, 480, 522, 919, 933
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 7 cut(s) 158, 265, 384, 399, 576, 763, 1029
FokI GGATG 3 cut(s) 10, 351, 881
Fsp4HI GCNGC 7 cut(s) 158, 265, 384, 399, 576, 763, 1029
FspBI CTAG 1 cut(s) 183
GlaI GCGC 3 cut(s) 135, 268, 318
GluI GCNGC 7 cut(s) 158, 265, 384, 399, 576, 763, 1029
GsuI CTGGAG 5 cut(s) 416, 523, 591, 829, 1023
HaeIII GGCC 4 cut(s) 515, 527, 798, 918
HapII CCGG 4 cut(s) 368, 402, 723, 795
HgaI GACGC 1 cut(s) 948
HhaI GCGC 3 cut(s) 136, 269, 319
Hin1I GRCGYC 1 cut(s) 959
Hin1II CATG 6 cut(s) 340, 363, 484, 526, 923, 937
Hin6I GCGC 3 cut(s) 134, 267, 317
HinP1I GCGC 3 cut(s) 134, 267, 317
HinfI GANTC 3 cut(s) 328, 476, 518
HpaII CCGG 4 cut(s) 368, 402, 723, 795
HphI GGTGA 5 cut(s) 56, 186, 775, 796, 877
Hpy166II GTNNAC 2 cut(s) 702, 708
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 6 cut(s) 236, 242, 368, 570, 898, 1002
Hpy8I GTNNAC 2 cut(s) 702, 708
Hpy99I CGWCG 6 cut(s) 157, 256, 259, 917, 964, 1016
HpyAV CCTTC 2 cut(s) 91, 790
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 4 cut(s) 333, 489, 594, 704
HpyCH4V TGCA 4 cut(s) 762, 848, 1031, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 768
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 4 cut(s) 333, 489, 594, 704
Hsp92I GRCGYC 1 cut(s) 959
Hsp92II CATG 6 cut(s) 340, 363, 484, 526, 923, 937
HspAI GCGC 3 cut(s) 134, 267, 317
Kpn2I TCCGGA 1 cut(s) 367
KpnI GGTACC 1 cut(s) 833
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 2 cut(s) 238, 909
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 4 cut(s) 489, 504, 572, 810
Lsp1109I GCAGC 5 cut(s) 385, 395, 587, 774, 1015
LweI GCATC 4 cut(s) 32, 129, 278, 1007
MaeI CTAG 1 cut(s) 183
MaeII ACGT 4 cut(s) 333, 489, 594, 704
MaeIII GTNAC 3 cut(s) 595, 802, 904
MalI GATC 2 cut(s) 240, 911
MboI GATC 2 cut(s) 238, 909
MboII GAAGA 5 cut(s) 116, 227, 317, 484, 598
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 1 cut(s) 946
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 2 cut(s) 322, 527
MmeI TCCRAC 2 cut(s) 480, 905
Mox20I TGGCCA 1 cut(s) 527
MroI TCCGGA 1 cut(s) 367
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 945
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 4 cut(s) 368, 402, 723, 795
MspR9I CCNGG 3 cut(s) 502, 800, 808
MvaI CCWGG 3 cut(s) 502, 800, 808
MwoI GCNNNNNNNGC 2 cut(s) 581, 768
NcoI CCATGG 1 cut(s) 522
NdeII GATC 2 cut(s) 238, 909
NlaIII CATG 6 cut(s) 340, 363, 484, 526, 923, 937
NlaIV GGNNCC 3 cut(s) 344, 406, 831
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 3 cut(s) 595, 802, 904
NspI RCATGY 1 cut(s) 363
NspV TTCGAA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PfeI GAWTC 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 7 cut(s) 159, 266, 385, 400, 577, 764, 1030
Ple19I CGATCG 1 cut(s) 912
PleI GAGTC 2 cut(s) 322, 526
PpsI GAGTC 2 cut(s) 322, 526
PshAI GACNNNNGTC 1 cut(s) 332
Psp6I CCWGG 3 cut(s) 500, 798, 806
PspEI GGTNACC 1 cut(s) 802
PspGI CCWGG 3 cut(s) 500, 798, 806
PspN4I GGNNCC 3 cut(s) 344, 406, 831
PspPI GGNCC 1 cut(s) 713
PstI CTGCAG 2 cut(s) 764, 1033
PvuI CGATCG 1 cut(s) 912
RsaI GTAC 5 cut(s) 437, 638, 677, 831, 893
RsaNI GTAC 5 cut(s) 436, 637, 676, 830, 892
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 945
SatI GCNGC 7 cut(s) 158, 265, 384, 399, 576, 763, 1029
Sau3AI GATC 2 cut(s) 238, 909
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 2 cut(s) 322, 527
ScrFI CCNGG 3 cut(s) 502, 800, 808
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 4 cut(s) 32, 129, 278, 1007
SfcI CTRYAG 2 cut(s) 760, 1029
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 713
Sse9I AATT 1 cut(s) 946
SsiI CCGC 6 cut(s) 98, 158, 264, 278, 769, 786
SspI AATATT 1 cut(s) 844
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 500, 798, 806
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 4 cut(s) 336, 492, 597, 707
TaqI TCGA 4 cut(s) 171, 912, 962, 1014
TasI AATT 1 cut(s) 946
TatI WGTACW 4 cut(s) 435, 636, 675, 891
TauI GCSGC 2 cut(s) 160, 267
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 945
Tru9I TTAA 1 cut(s) 945
TscAI CASTG 2 cut(s) 326, 867
TseFI GTSAC 3 cut(s) 595, 802, 904
TseI GCWGC 5 cut(s) 383, 398, 575, 762, 1028
Tsp45I GTSAC 3 cut(s) 595, 802, 904
TspDTI ATGAA 4 cut(s) 17, 110, 605, 922
TspGWI ACGGA 2 cut(s) 240, 899
TspRI CASTG 2 cut(s) 326, 867
VpaK11BI GGWCC 1 cut(s) 713
XagI CCTNNNNNAGG 1 cut(s) 201
XceI RCATGY 1 cut(s) 363
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.