RLG00000022400

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004816
Physical Location & Seq
Reverse (-)
83113797 .. 83115048
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022400

Sequence Viewer

Length: 1149 bp
ATGAACAACAGCAACCAAGAATCTCAACCACCTATGTACCCCTCCATTCCTCCTCCTCTCACAGCACACGAAGCAAACCCTTTTCTTCAATCTCATCCTACTCCCGCCCCAGATAATATCAATCCATTTTCTCAAACCACTAACCCACCAGAAGCCCCAAAACCTCAAACCCCCTCCATTACTGCCGACCACTTAGAAAAGTGGCAACCTGCTCCTCATGATCATGATCATCATCAAGATATTCCATCATCTTCATCTGAGCAAGTACCAGTAGCAGCAGCAGCAGCACCACCAACCTTTGACAGCGAAGATCTCAAAGCCAAGGCCGCGGCGGAGGCAGAGCCAGAGAAATGGGGCACTCACGTCATGGGACACCCGGCTGTCCCAACTTGCCACCCGGACAACAAAAAGGCAGCTCTATGGGGAGTTGCCGCCGATGGTACAGAAAAAGCTCAGAGCTTCCACTATCCGTACCTGCAGTACCAACCCATTGATCACAGCAACAAGGCCAGCAGCTCCAGCAATCCCGCTCAATCTATGCTCAATGTCTTCAACTCCTGGAGCCACAGAGCCGAGTCCATGGCCAACAACATCTGGCACAACCTTAAAACGGGGACATCTGTTTCCGGAGCAGCGTGGGCGAAGATGAACCTGCAGGCGAAAGCATTAACAGGAGGTGGATTTGAGGCTATCTACAAGCAGACATTTGCGACTTATCCCAACGAGCAGCTCCACAAATCATTCGCCTGTTATCTCTCCACATCCACCGGACCAGTCGCCGGAACTCTTTACCTGTCCAATATTCACACTGCTTTTTGCAGCGACCGGCCACTGTCATTTACTGCACCTTCCGGTCAGGTGACTTGGAGCTATTACAAGGTTATGGTGCCGCTGGCAAATGTTGCGACTATCAATCCAGTGGTGATGAGGGAGAATCCGACTGAGAAGTACCTCCAGATTGTGACCATTGACGGCCATGATTTCTGGTTCATGGGGTTTGTTAATTATGAAAAAGCATCCAAACATCTTACTCAGAGTATCTCCACTTTCGTGGCGTCTGGATTTGCAGTCCAACCTCCCCCATATGAAAAGCATGCTGGTGCTGAACATGATCATGATCATGATCACAAGAAGGGGGGTGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

383

Amino Acids

41.79

Weight (kDa)

6.12

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 233 - 345 1.5e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 217, 483, 660
AccB1I GGYRCC 1 cut(s) 886
AccBSI CCGCTC 1 cut(s) 530
AccII CGCG 1 cut(s) 329
AccIII TCCGGA 1 cut(s) 626
AciI CCGC 7 cut(s) 105, 327, 329, 332, 432, 528, 890
AcoI YGGCCR 3 cut(s) 582, 827, 973
AcyI GRCGYC 1 cut(s) 1055
AfaI GTAC 6 cut(s) 38, 267, 442, 473, 482, 950
AfiI CCNNNNNNNGG 2 cut(s) 610, 779
AgsI TTSAA 2 cut(s) 89, 553
AjiI CACGTC 1 cut(s) 364
AjnI CCWGG 1 cut(s) 557
AleI CACNNNNGTG 1 cut(s) 1049
AluBI AGCT 6 cut(s) 416, 452, 459, 516, 730, 870
AluI AGCT 6 cut(s) 416, 452, 459, 516, 730, 870
Aor13HI TCCGGA 1 cut(s) 626
AoxI GGCC 5 cut(s) 324, 507, 582, 827, 973
ApeKI GCWGC 9 cut(s) 275, 278, 281, 284, 413, 513, 632, 727, 819
AspS9I GGNCC 1 cut(s) 770
AsuC2I CCSGG 2 cut(s) 377, 398
AsuHPI GGTGA 2 cut(s) 871, 934
AvaII GGWCC 1 cut(s) 770
BaeGI GKGCMC 1 cut(s) 359
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BalI TGGCCA 1 cut(s) 584
BanI GGYRCC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 541
BbvI GCAGC 9 cut(s) 287, 290, 293, 296, 425, 525, 644, 739, 831
BccI CCATC 2 cut(s) 253, 431
BceAI ACGGC 1 cut(s) 988
BciT130I CCWGG 1 cut(s) 559
BclI TGATCA 6 cut(s) 220, 226, 493, 1111, 1117, 1123
BcnI CCSGG 2 cut(s) 377, 398
BfmI CTRYAG 2 cut(s) 476, 653
BfuAI ACCTGC 3 cut(s) 217, 483, 660
BglII AGATCT 1 cut(s) 310
Bme1390I CCNGG 3 cut(s) 377, 398, 559
Bme18I GGWCC 1 cut(s) 770
BmgBI CACGTC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 770
BmiI GGNNCC 2 cut(s) 563, 888
BmrFI CCNGG 3 cut(s) 377, 398, 559
BmsI GCATC 1 cut(s) 1025
BpiI GAAGAC 1 cut(s) 541
BpmI CTGGAG 3 cut(s) 502, 580, 938
BpuMI CCSGG 2 cut(s) 377, 398
BsaBI GATNNNNATC 4 cut(s) 225, 231, 1116, 1122
BsaHI GRCGYC 1 cut(s) 1055
BsaJI CCNNGG 3 cut(s) 321, 327, 579
BsaWI WCCGGW 3 cut(s) 626, 767, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 779
Bse118I RCCGGY 1 cut(s) 825
Bse1I ACTGG 3 cut(s) 269, 773, 917
Bse8I GATNNNNATC 4 cut(s) 225, 231, 1116, 1122
BseAI TCCGGA 1 cut(s) 626
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 3 cut(s) 321, 327, 579
BseGI GGATG 3 cut(s) 94, 761, 1016
BseJI GATNNNNATC 4 cut(s) 225, 231, 1116, 1122
BseLI CCNNNNNNNGG 2 cut(s) 610, 779
BseMII CTCAG 4 cut(s) 249, 467, 933, 1046
BseNI ACTGG 3 cut(s) 269, 773, 917
BseRI GAGGAG 3 cut(s) 42, 45, 204
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 9 cut(s) 287, 290, 293, 296, 425, 525, 644, 739, 831
BsgI GTGCAG 1 cut(s) 828
Bsh1236I CGCG 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 826
BshFI GGCC 5 cut(s) 326, 509, 584, 829, 975
BshNI GGYRCC 1 cut(s) 886
BsiEI CGRYCG 1 cut(s) 826
BsiSI CCGG 7 cut(s) 377, 398, 627, 768, 780, 826, 852
BslFI GGGAC 3 cut(s) 368, 384, 628
BslI CCNNNNNNNGG 2 cut(s) 610, 779
BsmFI GGGAC 3 cut(s) 368, 384, 628
BsnI GGCC 5 cut(s) 326, 509, 584, 829, 975
Bsp1286I GDGCHC 1 cut(s) 359
Bsp13I TCCGGA 1 cut(s) 626
Bsp143I GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 7 cut(s) 105, 327, 329, 332, 432, 528, 890
BspANI GGCC 5 cut(s) 326, 509, 584, 829, 975
BspCNI CTCAG 4 cut(s) 250, 466, 934, 1045
BspEI TCCGGA 1 cut(s) 626
BspFNI CGCG 1 cut(s) 329
BspHI TCATGA 4 cut(s) 217, 223, 1114, 1120
BspLI GGNNCC 2 cut(s) 563, 888
BspMAI CTGCAG 2 cut(s) 480, 657
BspMI ACCTGC 3 cut(s) 217, 483, 660
BspT107I GGYRCC 1 cut(s) 886
BsrBI CCGCTC 1 cut(s) 530
BsrFI RCCGGY 1 cut(s) 825
BsrI ACTGG 3 cut(s) 269, 773, 917
BssAI RCCGGY 1 cut(s) 825
BssECI CCNNGG 3 cut(s) 321, 327, 579
BssMI GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
BssNI GRCGYC 1 cut(s) 1055
BssT1I CCWWGG 2 cut(s) 321, 579
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 834
BstACI GRCGYC 1 cut(s) 1055
BstAPI GCANNNNNTGC 1 cut(s) 902
BstC8I GCNNGC 4 cut(s) 511, 657, 894, 1095
BstDEI CTNAG 5 cut(s) 193, 258, 453, 942, 1032
BstDSI CCRYGG 2 cut(s) 327, 579
BstF5I GGATG 3 cut(s) 94, 761, 1016
BstFNI CGCG 1 cut(s) 329
BstKTI GATC 7 cut(s) 223, 229, 313, 496, 1114, 1120, 1126
BstMBI GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
BstMCI CGRYCG 1 cut(s) 826
BstMWI GCNNNNNNNGC 8 cut(s) 71, 281, 284, 326, 335, 519, 638, 902
BstNI CCWGG 1 cut(s) 559
BstNSI RCATGY 1 cut(s) 1097
BstSCI CCNGG 3 cut(s) 375, 396, 557
BstSFI CTRYAG 2 cut(s) 476, 653
BstSLI GKGCMC 1 cut(s) 359
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 9 cut(s) 287, 290, 293, 296, 425, 525, 644, 739, 831
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 310
BstXI CCANNNNNNTGG 2 cut(s) 351, 1051
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 5 cut(s) 326, 509, 584, 829, 975
BtgI CCRYGG 2 cut(s) 327, 579
BtrI CACGTC 1 cut(s) 364
BtsCI GGATG 3 cut(s) 94, 761, 1016
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 3 cut(s) 807, 830, 924
BveI ACCTGC 3 cut(s) 217, 483, 660
Cac8I GCNNGC 4 cut(s) 511, 657, 894, 1095
CciI TCATGA 4 cut(s) 217, 223, 1114, 1120
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 1 cut(s) 770
Cfr42I CCGCGG 1 cut(s) 330
CseI GACGC 1 cut(s) 1044
Csp6I GTAC 6 cut(s) 37, 266, 441, 472, 481, 949
CviQI GTAC 6 cut(s) 37, 266, 441, 472, 481, 949
DdeI CTNAG 5 cut(s) 193, 258, 453, 942, 1032
DpnI GATC 7 cut(s) 222, 228, 312, 495, 1113, 1119, 1125
DpnII GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
EaeI YGGCCR 3 cut(s) 582, 827, 973
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 321, 579
Eco47I GGWCC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 557
EcoT14I CCWWGG 2 cut(s) 321, 579
ErhI CCWWGG 2 cut(s) 321, 579
FaqI GGGAC 3 cut(s) 368, 384, 628
FauI CCCGC 2 cut(s) 112, 535
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 6 cut(s) 220, 226, 493, 1111, 1117, 1123
FokI GGATG 3 cut(s) 81, 748, 1003
GsuI CTGGAG 3 cut(s) 502, 580, 938
HaeIII GGCC 5 cut(s) 326, 509, 584, 829, 975
HapII CCGG 7 cut(s) 377, 398, 627, 768, 780, 826, 852
HgaI GACGC 1 cut(s) 1044
Hin1I GRCGYC 1 cut(s) 1055
HinfI GANTC 3 cut(s) 20, 575, 934
HpaII CCGG 7 cut(s) 377, 398, 627, 768, 780, 826, 852
HphI GGTGA 2 cut(s) 871, 934
Hpy188I TCNGA 4 cut(s) 259, 456, 939, 1035
Hpy188III TCNNGA 8 cut(s) 218, 224, 236, 627, 955, 1059, 1115, 1121
HpyAV CCTTC 2 cut(s) 858, 1126
HpyCH4III ACNGT 1 cut(s) 834
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 5 cut(s) 478, 655, 819, 845, 1067
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 281, 284, 326, 335, 519, 638, 902
HpyF3I CTNAG 5 cut(s) 193, 258, 453, 942, 1032
HpySE526I ACGT 1 cut(s) 363
Hsp92I GRCGYC 1 cut(s) 1055
Kpn2I TCCGGA 1 cut(s) 626
Ksp22I TGATCA 6 cut(s) 220, 226, 493, 1111, 1117, 1123
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
LmnI GCTCC 6 cut(s) 217, 521, 561, 629, 735, 867
Lsp1109I GCAGC 9 cut(s) 287, 290, 293, 296, 425, 525, 644, 739, 831
LweI GCATC 1 cut(s) 1025
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 3 cut(s) 859, 961, 1139
MalI GATC 7 cut(s) 222, 228, 312, 495, 1113, 1119, 1125
MbiI CCGCTC 1 cut(s) 530
MboI GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
MboII GAAGA 5 cut(s) 77, 243, 320, 541, 655
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 1 cut(s) 1003
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 962, 1096
Mox20I TGGCCA 1 cut(s) 584
MroI TCCGGA 1 cut(s) 626
MscI TGGCCA 1 cut(s) 584
MseI TTAA 3 cut(s) 606, 668, 1002
MslI CAYNNNNRTG 5 cut(s) 222, 1049, 1098, 1113, 1119
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 329, 892
MspI CCGG 7 cut(s) 377, 398, 627, 768, 780, 826, 852
MspR9I CCNGG 3 cut(s) 377, 398, 559
MvaI CCWGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 8 cut(s) 71, 281, 284, 326, 335, 519, 638, 902
NciI CCSGG 2 cut(s) 377, 398
NcoI CCATGG 1 cut(s) 579
NdeI CATATG 1 cut(s) 1084
NdeII GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
NlaIV GGNNCC 2 cut(s) 563, 888
NmeAIII GCCGAG 1 cut(s) 598
NmuCI GTSAC 3 cut(s) 859, 961, 1139
NspI RCATGY 1 cut(s) 1097
OliI CACNNNNGTG 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 4 cut(s) 217, 223, 1114, 1120
PfeI GAWTC 2 cut(s) 20, 934
PfoI TCCNGGA 1 cut(s) 557
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 2 cut(s) 563, 888
PspPI GGNCC 1 cut(s) 770
PstI CTGCAG 2 cut(s) 480, 657
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 6 cut(s) 38, 267, 442, 473, 482, 950
RsaNI GTAC 6 cut(s) 37, 266, 441, 472, 481, 949
RseI CAYNNNNRTG 5 cut(s) 222, 1049, 1098, 1113, 1119
SacII CCGCGG 1 cut(s) 330
SaqAI TTAA 3 cut(s) 606, 668, 1002
Sau3AI GATC 7 cut(s) 220, 226, 310, 493, 1111, 1117, 1123
Sau96I GGNCC 1 cut(s) 770
SbfI CCTGCAGG 1 cut(s) 657
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 3 cut(s) 377, 398, 559
SdaI CCTGCAGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 359
SfaNI GCATC 1 cut(s) 1025
SfcI CTRYAG 2 cut(s) 476, 653
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 1 cut(s) 770
SmiMI CAYNNNNRTG 5 cut(s) 222, 1049, 1098, 1113, 1119
SphI GCATGC 1 cut(s) 1097
Sse8387I CCTGCAGG 1 cut(s) 657
Sse9I AATT 1 cut(s) 1003
SsiI CCGC 7 cut(s) 105, 327, 329, 332, 432, 528, 890
SspI AATATT 1 cut(s) 802
StyD4I CCNGG 3 cut(s) 375, 396, 557
StyI CCWWGG 2 cut(s) 321, 579
TaaI ACNGT 1 cut(s) 834
TaiI ACGT 1 cut(s) 366
TasI AATT 1 cut(s) 1003
TauI GCSGC 4 cut(s) 329, 332, 434, 892
TfiI GAWTC 2 cut(s) 20, 934
Tru1I TTAA 3 cut(s) 606, 668, 1002
Tru9I TTAA 3 cut(s) 606, 668, 1002
TscAI CASTG 3 cut(s) 814, 837, 924
TseFI GTSAC 3 cut(s) 859, 961, 1139
TseI GCWGC 9 cut(s) 275, 278, 281, 284, 413, 513, 632, 727, 819
Tsp45I GTSAC 3 cut(s) 859, 961, 1139
TspDTI ATGAA 6 cut(s) 17, 243, 662, 979, 1023, 1101
TspGWI ACGGA 1 cut(s) 459
TspRI CASTG 3 cut(s) 814, 837, 924
VpaK11BI GGWCC 1 cut(s) 770
XceI RCATGY 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.