RchiOBHm_Chr4g0429311

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004816
Physical Location & Seq
Forward (+)
54115981 .. 54117364
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39813

Sequence Viewer

Length: 873 bp
ATGTCGAGCAGACCGGAAGACCAGCTTTCTCCCCGGGAGTCTTATCCTCCCACCACAACCGTCCCCGACATTCACGCCTCGCAACCGCCGCCGCCGCCGCCTCCGCCGTCGGAGGAGGACGCCAAGAAATGGGGGACCCACATAATGGGAACTCCCGCAGCTCCGAACGTCCACCCGGATAACCAGAAAGCTGCTCTATGGAAAGCCGCTGACCACCAGCAAATCCCGCAGCAACCGCTCGAAACACAACAGCAACAACAGCCGTACGTTCAGTACTCTCCGATCAACAAGCCGACCAACAACCCCTTCGAGCCGGTGATCAACCTCTTCAACTCGTGGGGAAACAAGGCCGAGACCATCGCCCGGAACATCTGGCACAACCTCAAAACAGGGCCCTCTGTTTCCGAGGCCGCCTGGGGAAAAGTGAACTTGACGGCCAAGGCGATCACGGAAGGCGGGTTTGAGTCTCTGTTTAAGCAGATTTTCGCTACTGATCCCAACGAGAGGTTGAAGAAGACTTTCGCTTGCTATCTTTCGACTTCCACCGGACCTGTGGCCGGGACTCTGTATCTGTCGACGGCAAGAATCGGTTTTTGCAGCGACCGGCCGTTGACTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATTCCTATGACGAATGTAGGCAGAGTGAACCCTGTGGTGATTACGGAGACCCCGACGCCGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTCGAGAAAGCTTCGCATCACGTGCTAGACAGTGTGTCGAATTTCAGAGCAGTTGGGACCAATGAAGTGCAACCTGTTGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

290

Amino Acids

32.13

Weight (kDa)

6.45

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 157 - 266 4.9e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 129, 145
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 575
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 3 cut(s) 435, 555, 605
AcsI RAATTY 1 cut(s) 826
AcuI CTGAAG 1 cut(s) 719
AcvI CACGTG 1 cut(s) 808
AcyI GRCGYC 2 cut(s) 120, 719
AfaI GTAC 3 cut(s) 266, 275, 731
AfiI CCNNNNNNNGG 6 cut(s) 129, 145, 363, 504, 557, 721
AgsI TTSAA 2 cut(s) 331, 511
AhdI GACNNNNNGTC 1 cut(s) 820
AjnI CCWGG 1 cut(s) 413
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 5 cut(s) 25, 161, 191, 648, 797
AluI AGCT 5 cut(s) 25, 161, 191, 648, 797
Alw26I GTCTC 3 cut(s) 347, 471, 704
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 6 cut(s) 348, 392, 408, 435, 555, 605
ApaI GGGCCC 1 cut(s) 396
ApeKI GCWGC 4 cut(s) 158, 191, 229, 597
ApoI RAATTY 1 cut(s) 826
Asp700I GAANNNNTTC 1 cut(s) 518
AspS9I GGNCC 5 cut(s) 135, 392, 393, 548, 843
AsuC2I CCSGG 5 cut(s) 34, 35, 176, 364, 559
AsuHPI GGTGA 4 cut(s) 328, 670, 712, 736
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 3 cut(s) 135, 548, 843
BaeGI GKGCMC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 334
BbrPI CACGTG 1 cut(s) 808
BbsI GAAGAC 2 cut(s) 24, 521
BbvI GCAGC 4 cut(s) 170, 178, 241, 609
BccI CCATC 2 cut(s) 365, 746
BceAI ACGGC 5 cut(s) 91, 247, 450, 592, 594
BciT130I CCWGG 1 cut(s) 415
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 5 cut(s) 34, 35, 176, 364, 559
BcoDI GTCTC 3 cut(s) 347, 471, 704
BfaI CTAG 1 cut(s) 812
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 6 cut(s) 34, 35, 176, 364, 415, 559
Bme18I GGWCC 3 cut(s) 135, 548, 843
BmeRI GACNNNNNGTC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 135, 392, 393, 548, 843
BmiI GGNNCC 4 cut(s) 136, 137, 394, 844
BmrFI CCNGG 6 cut(s) 34, 35, 176, 364, 415, 559
BmsI GCATC 1 cut(s) 811
BpiI GAAGAC 2 cut(s) 24, 521
BpuMI CCSGG 5 cut(s) 34, 35, 176, 364, 559
BsaAI YACGTR 1 cut(s) 808
BsaHI GRCGYC 2 cut(s) 120, 719
BsaI GGTCTC 2 cut(s) 347, 704
BsaJI CCNNGG 5 cut(s) 32, 33, 405, 414, 438
BsaWI WCCGGW 2 cut(s) 13, 545
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 145, 363, 504, 557, 721
Bse118I RCCGGY 2 cut(s) 313, 603
BseBI CCWGG 1 cut(s) 415
BseDI CCNNGG 5 cut(s) 32, 33, 405, 414, 438
BseLI CCNNNNNNNGG 6 cut(s) 129, 145, 363, 504, 557, 721
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 396
BseX3I CGGCCG 1 cut(s) 605
BseXI GCAGC 4 cut(s) 170, 178, 241, 609
Bsh1285I CGRYCG 2 cut(s) 604, 608
BshFI GGCC 6 cut(s) 350, 394, 410, 437, 557, 607
BsiEI CGRYCG 2 cut(s) 604, 608
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 9 cut(s) 14, 34, 176, 314, 364, 546, 558, 604, 722
BsiWI CGTACG 1 cut(s) 264
BslFI GGGAC 4 cut(s) 47, 148, 574, 856
BslI CCNNNNNNNGG 6 cut(s) 129, 145, 363, 504, 557, 721
BsmAI GTCTC 3 cut(s) 347, 471, 704
BsmFI GGGAC 4 cut(s) 47, 148, 574, 856
BsnI GGCC 6 cut(s) 350, 394, 410, 437, 557, 607
Bso31I GGTCTC 2 cut(s) 347, 704
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 4 cut(s) 282, 318, 444, 493
BspANI GGCC 6 cut(s) 350, 394, 410, 437, 557, 607
BspLI GGNNCC 4 cut(s) 136, 137, 394, 844
BspPI GGATC 1 cut(s) 488
BspTNI GGTCTC 2 cut(s) 347, 704
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 2 cut(s) 313, 603
BssAI RCCGGY 2 cut(s) 313, 603
BssECI CCNNGG 5 cut(s) 32, 33, 405, 414, 438
BssMI GATC 4 cut(s) 282, 318, 444, 493
BssNI GRCGYC 2 cut(s) 120, 719
BssSI CACGAG 1 cut(s) 334
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 2 cut(s) 61, 818
Bst6I CTCTTC 1 cut(s) 332
BstACI GRCGYC 2 cut(s) 120, 719
BstAPI GCANNNNNTGC 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 1 cut(s) 526
BstKTI GATC 4 cut(s) 285, 321, 447, 496
BstMAI GTCTC 3 cut(s) 347, 471, 704
BstMBI GATC 4 cut(s) 282, 318, 444, 493
BstMCI CGRYCG 2 cut(s) 604, 608
BstMWI GCNNNNNNNGC 8 cut(s) 88, 94, 97, 103, 226, 235, 259, 808
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 6 cut(s) 32, 33, 174, 362, 413, 557
BstSLI GKGCMC 1 cut(s) 396
BstV1I GCAGC 4 cut(s) 170, 178, 241, 609
BstV2I GAAGAC 2 cut(s) 24, 521
BstZI CGGCCG 1 cut(s) 605
BsuRI GGCC 6 cut(s) 350, 394, 410, 437, 557, 607
BtgZI GCGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 823
Cac8I GCNNGC 1 cut(s) 526
Cfr10I RCCGGY 2 cut(s) 313, 603
Cfr13I GGNCC 5 cut(s) 135, 392, 393, 548, 843
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 2 cut(s) 128, 727
Csp6I GTAC 3 cut(s) 265, 274, 730
CviAII CATG 1 cut(s) 758
CviQI GTAC 3 cut(s) 265, 274, 730
DpnI GATC 4 cut(s) 284, 320, 446, 495
DpnII GATC 4 cut(s) 282, 318, 444, 493
DriI GACNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 3 cut(s) 435, 555, 605
EagI CGGCCG 1 cut(s) 605
Eam1104I CTCTTC 1 cut(s) 332
Eam1105I GACNNNNNGTC 1 cut(s) 820
EarI CTCTTC 1 cut(s) 332
EciI GGCGGA 1 cut(s) 93
EclXI CGGCCG 1 cut(s) 605
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 2 cut(s) 347, 704
Eco47I GGWCC 3 cut(s) 135, 548, 843
Eco52I CGGCCG 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 719
Eco72I CACGTG 1 cut(s) 808
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 3 cut(s) 135, 392, 393
EcoRII CCWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 1 cut(s) 761
FaiI YATR 5 cut(s) 143, 199, 671, 759, 773
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 4 cut(s) 47, 148, 574, 856
FatI CATG 1 cut(s) 757
FauI CCCGC 3 cut(s) 163, 234, 449
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 1 cut(s) 575
FriOI GRGCYC 1 cut(s) 396
FspBI CTAG 1 cut(s) 812
HaeIII GGCC 6 cut(s) 350, 394, 410, 437, 557, 607
HapII CCGG 9 cut(s) 14, 34, 176, 314, 364, 546, 558, 604, 722
HgaI GACGC 2 cut(s) 128, 727
Hin1I GRCGYC 2 cut(s) 120, 719
Hin1II CATG 1 cut(s) 761
HincII GTYRAC 3 cut(s) 576, 612, 784
HindII GTYRAC 3 cut(s) 576, 612, 784
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 5 cut(s) 38, 464, 562, 585, 664
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 9 cut(s) 14, 34, 176, 314, 364, 546, 558, 604, 722
HphI GGTGA 4 cut(s) 328, 670, 712, 736
Hpy166II GTNNAC 6 cut(s) 172, 427, 576, 612, 691, 784
Hpy188I TCNGA 6 cut(s) 112, 165, 282, 406, 738, 833
Hpy188III TCNNGA 2 cut(s) 630, 790
Hpy8I GTNNAC 6 cut(s) 172, 427, 576, 612, 691, 784
Hpy99I CGWCG 3 cut(s) 112, 580, 721
HpyAV CCTTC 3 cut(s) 316, 446, 743
HpyCH4III ACNGT 2 cut(s) 61, 818
HpyCH4IV ACGT 3 cut(s) 168, 267, 807
HpyCH4V TGCA 2 cut(s) 597, 856
HpyF10VI GCNNNNNNNGC 8 cut(s) 88, 94, 97, 103, 226, 235, 259, 808
HpySE526I ACGT 3 cut(s) 168, 267, 807
Hsp92I GRCGYC 2 cut(s) 120, 719
Hsp92II CATG 1 cut(s) 761
KflI GGGWCCC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 318
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 4 cut(s) 282, 318, 444, 493
LmnI GCTCC 3 cut(s) 166, 628, 645
Lsp1109I GCAGC 4 cut(s) 170, 178, 241, 609
LweI GCATC 1 cut(s) 811
MaeI CTAG 1 cut(s) 812
MaeII ACGT 3 cut(s) 168, 267, 807
MaeIII GTNAC 1 cut(s) 742
MalI GATC 4 cut(s) 284, 320, 446, 495
MbiI CCGCTC 1 cut(s) 238
MboI GATC 4 cut(s) 282, 318, 444, 493
MboII GAAGA 4 cut(s) 29, 319, 523, 526
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 1 cut(s) 826
MlyI GAGTC 3 cut(s) 47, 473, 556
MmeI TCCRAC 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 2 cut(s) 474, 783
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 9 cut(s) 14, 34, 176, 314, 364, 546, 558, 604, 722
MspR9I CCNGG 6 cut(s) 34, 35, 176, 364, 415, 559
MvaI CCWGG 1 cut(s) 415
MwoI GCNNNNNNNGC 8 cut(s) 88, 94, 97, 103, 226, 235, 259, 808
NciI CCSGG 5 cut(s) 34, 35, 176, 364, 559
NdeII GATC 4 cut(s) 282, 318, 444, 493
NlaIII CATG 1 cut(s) 761
NlaIV GGNNCC 4 cut(s) 136, 137, 394, 844
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 742
PcsI WCGNNNNNNNCGW 2 cut(s) 440, 713
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 585, 664
Pfl23II CGTACG 1 cut(s) 264
PflMI CCANNNNNTGG 2 cut(s) 129, 145
PleI GAGTC 3 cut(s) 46, 472, 556
PmaCI CACGTG 1 cut(s) 808
PmlI CACGTG 1 cut(s) 808
PpsI GAGTC 3 cut(s) 46, 472, 556
Ppu21I YACGTR 1 cut(s) 808
PpuMI RGGWCCY 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 413
PspCI CACGTG 1 cut(s) 808
PspGI CCWGG 1 cut(s) 413
PspLI CGTACG 1 cut(s) 264
PspN4I GGNNCC 4 cut(s) 136, 137, 394, 844
PspOMI GGGCCC 1 cut(s) 392
PspPI GGNCC 5 cut(s) 135, 392, 393, 548, 843
PspPPI RGGWCCY 1 cut(s) 135
RsaI GTAC 3 cut(s) 266, 275, 731
RsaNI GTAC 3 cut(s) 265, 274, 730
SalI GTCGAC 1 cut(s) 574
SaqAI TTAA 2 cut(s) 474, 783
Sau3AI GATC 4 cut(s) 282, 318, 444, 493
Sau96I GGNCC 5 cut(s) 135, 392, 393, 548, 843
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 3 cut(s) 47, 473, 556
ScrFI CCNGG 6 cut(s) 34, 35, 176, 364, 415, 559
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 1 cut(s) 811
SinI GGWCC 3 cut(s) 135, 548, 843
SmaI CCCGGG 1 cut(s) 35
Sse9I AATT 1 cut(s) 826
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 6 cut(s) 32, 33, 174, 362, 413, 557
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 61, 818
TaiI ACGT 3 cut(s) 171, 270, 810
TaqI TCGA 7 cut(s) 5, 240, 309, 536, 575, 789, 824
TasI AATT 1 cut(s) 826
TatI WGTACW 1 cut(s) 273
TauI GCSGC 6 cut(s) 91, 94, 97, 100, 209, 413
TfiI GAWTC 2 cut(s) 585, 664
Tru1I TTAA 2 cut(s) 474, 783
Tru9I TTAA 2 cut(s) 474, 783
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 4 cut(s) 158, 191, 229, 597
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 1 cut(s) 864
TspGWI ACGGA 3 cut(s) 464, 615, 722
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 1 cut(s) 823
Van91I CCANNNNNTGG 2 cut(s) 129, 145
VpaK11BI GGWCC 3 cut(s) 135, 548, 843
XapI RAATTY 1 cut(s) 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 1 cut(s) 812
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.