FvH4_6g54120

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004816
Physical Location & Seq
Reverse (-)
39541462 .. 39543303
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54120.t1

Sequence Viewer

Length: 1176 bp
ATGAACAACAACCACCAAGAATCTCAACCACCTATGTACCCCTCCATTCCTCCGCCTCTCACACAACAGGAAGCAAACCCTTTTCATCAATCTCATCCCACTCCCGCCCCAGAAGAAATCAATCCATTTTATCAAGCCCCTGAAGCCCCTAAACCTCAGACCCCCTCCATCACTGCTGACCACTTAGAAAAATGGCAACCTGCTCCTCCTCCTCCTCCTCCTCCTCCTCAAGATGTTGCATCATCATCTTCATCTGAGCAAGTACCAGTAGCAACACCACCACCCACCTTTGACAGTGAAGATCTCAAAGCCAAGGCCGCGGCGGAGGCAGAGCCAGAGAAATGGGGAACTCACGTCATGGGACACCCTGCTGTCCCAACATGCCACCCGGACAACAAAAAGGCAGCTCTCTGGGGAGTTAACGCCGATGGTCCGGAAAAGGCACAGAGCTTCCACTATCCCTACCTCCAGTACCAACCCATCGATAACAACAACAACAACAACAAGGCCAGCTCCAGCAATGCCCCACAATCTATGCTCAATGTCTTCAACTCCTGGAGCCACAAGGCCGAGTCCATGGCCAACAACATCTGGCGCAACCTTAAAACTGGGACATCAGTGTCGGGAGCAGCGTGGGCGAAGATGAACCTGCAAGCCAAAGCATTAACAGGAGGTGGATTTGAGGCTCTGTACAAGCAGTCATTTGCGACTTATCCCAACGAGAAGCTCCACAAATCATTCGCCTGCTATCTCTCCACATCCACCGGACCAGTCGCCGGAACTCTTTACCTCTCCAATATTCACACCGCTTTTTGCAGCGACCGGCCTCTGTCATTCACGGCGCCTTCCGGTCAGGTGACTTGGAGCTACTACAAGGTCATGGTGCCATTGGCAAATGTTGCGACTATCAATCCAGTAGTGATGAGGGAGAATACGACGGAGAAGTACCTCCAGATTTTGACCATTGACGGCCATGATTTCTGGTTCATGGGGTTTGTTAATTATGAAAAGGCATCCAAGCATCTTACCCAGAGTGTCTCCACTTTCGTAGCGTCTGGATTTGCAGTCCAGCCTCCCACATATGAAGGGCCTGCTGCTGCTGAATATGATCATGATCACAAGAAGGGGCCTGCTGCTGCTGAACATGATCATGATCACAAGAAGGGGGGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

392

Amino Acids

42.77

Weight (kDa)

6.04

Isoelectric Point (pI)

61.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 232 - 344 1.2e-14 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 619
Acc36I ACCTGC 2 cut(s) 208, 657
AccB1I GGYRCC 2 cut(s) 841, 883
AccII CGCG 1 cut(s) 320
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 6 cut(s) 53, 105, 318, 320, 323, 807
AcoI YGGCCR 2 cut(s) 579, 970
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 842
AfaI GTAC 5 cut(s) 38, 264, 473, 692, 947
AfiI CCNNNNNNNGG 1 cut(s) 776
AgsI TTSAA 1 cut(s) 550
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 554
AluBI AGCT 5 cut(s) 407, 450, 513, 727, 867
AluI AGCT 5 cut(s) 407, 450, 513, 727, 867
Alw26I GTCTC 1 cut(s) 1042
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 8 cut(s) 315, 507, 567, 579, 824, 970, 1088, 1127
ApeKI GCWGC 7 cut(s) 404, 629, 816, 1094, 1097, 1133, 1136
AspLEI GCGC 2 cut(s) 597, 844
AspS9I GGNCC 4 cut(s) 431, 767, 1088, 1127
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 868
AvaII GGWCC 2 cut(s) 431, 767
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 2 cut(s) 841, 883
BbsI GAAGAC 1 cut(s) 538
BbvI GCAGC 7 cut(s) 416, 641, 828, 1081, 1084, 1120, 1123
BccI CCATC 3 cut(s) 176, 422, 488
BceAI ACGGC 2 cut(s) 855, 985
BciT130I CCWGG 1 cut(s) 556
BclI TGATCA 4 cut(s) 1108, 1114, 1147, 1153
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 1042
BfaI CTAG 1 cut(s) 1174
BfoI RGCGCY 1 cut(s) 845
BfuAI ACCTGC 2 cut(s) 208, 657
BglII AGATCT 1 cut(s) 301
BisI GCNGC 9 cut(s) 318, 321, 405, 630, 817, 1095, 1098, 1134, 1137
BlsI GCNGC 9 cut(s) 319, 322, 406, 631, 818, 1096, 1099, 1135, 1138
Bme1390I CCNGG 2 cut(s) 389, 556
Bme18I GGWCC 2 cut(s) 431, 767
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 4 cut(s) 431, 767, 1088, 1127
BmiI GGNNCC 4 cut(s) 560, 843, 885, 1128
BmrFI CCNGG 2 cut(s) 389, 556
BmrI ACTGGG 1 cut(s) 618
BmsI GCATC 3 cut(s) 248, 1022, 1030
BmuI ACTGGG 1 cut(s) 618
BpiI GAAGAC 1 cut(s) 538
BpmI CTGGAG 4 cut(s) 452, 499, 577, 935
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 483
BsaBI GATNNNNATC 2 cut(s) 1113, 1152
BsaHI GRCGYC 1 cut(s) 842
BsaJI CCNNGG 3 cut(s) 312, 318, 576
BsaWI WCCGGW 3 cut(s) 433, 764, 848
Bsc4I CCNNNNNNNGG 1 cut(s) 776
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 5 cut(s) 266, 469, 613, 770, 914
Bse3DI GCAATG 1 cut(s) 526
Bse8I GATNNNNATC 2 cut(s) 1113, 1152
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 556
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 3 cut(s) 312, 318, 576
BseGI GGATG 3 cut(s) 94, 758, 1013
BseJI GATNNNNATC 2 cut(s) 1113, 1152
BseLI CCNNNNNNNGG 1 cut(s) 776
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 2 cut(s) 170, 246
BseNI ACTGG 5 cut(s) 266, 469, 613, 770, 914
BseRI GAGGAG 8 cut(s) 195, 198, 201, 204, 207, 210, 213, 216
BseXI GCAGC 7 cut(s) 416, 641, 828, 1081, 1084, 1120, 1123
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 823
BshFI GGCC 8 cut(s) 317, 509, 569, 581, 826, 972, 1090, 1129
BshNI GGYRCC 2 cut(s) 841, 883
BshVI ATCGAT 1 cut(s) 483
BsiEI CGRYCG 1 cut(s) 823
BsiSI CCGG 6 cut(s) 389, 434, 765, 777, 823, 849
BslFI GGGAC 3 cut(s) 359, 375, 625
BslI CCNNNNNNNGG 1 cut(s) 776
BsmAI GTCTC 1 cut(s) 1042
BsmFI GGGAC 3 cut(s) 359, 375, 625
BsnI GGCC 8 cut(s) 317, 509, 569, 581, 826, 972, 1090, 1129
Bsp13I TCCGGA 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 6 cut(s) 53, 105, 318, 320, 323, 807
BspANI GGCC 8 cut(s) 317, 509, 569, 581, 826, 972, 1090, 1129
BspCNI CTCAG 2 cut(s) 169, 247
BspDI ATCGAT 1 cut(s) 483
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 2 cut(s) 1111, 1150
BspLI GGNNCC 4 cut(s) 560, 843, 885, 1128
BspMI ACCTGC 2 cut(s) 208, 657
BspT107I GGYRCC 2 cut(s) 841, 883
BsrDI GCAATG 1 cut(s) 526
BsrFI RCCGGY 1 cut(s) 822
BsrGI TGTACA 1 cut(s) 690
BsrI ACTGG 5 cut(s) 266, 469, 613, 770, 914
BssAI RCCGGY 1 cut(s) 822
BssECI CCNNGG 3 cut(s) 312, 318, 576
BssMI GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
BssNI GRCGYC 1 cut(s) 842
BssT1I CCWWGG 2 cut(s) 312, 576
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 842
BstAPI GCANNNNNTGC 1 cut(s) 899
BstAUI TGTACA 1 cut(s) 690
BstC8I GCNNGC 5 cut(s) 511, 654, 745, 1092, 1131
BstDEI CTNAG 3 cut(s) 156, 184, 255
BstDSI CCRYGG 2 cut(s) 318, 576
BstF5I GGATG 3 cut(s) 94, 758, 1013
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 2 cut(s) 597, 844
BstKTI GATC 5 cut(s) 304, 1111, 1117, 1150, 1156
BstMAI GTCTC 1 cut(s) 1042
BstMBI GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
BstMCI CGRYCG 1 cut(s) 823
BstMWI GCNNNNNNNGC 5 cut(s) 143, 317, 326, 635, 899
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 2 cut(s) 387, 554
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 7 cut(s) 416, 641, 828, 1081, 1084, 1120, 1123
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 342
BstYI RGATCY 1 cut(s) 301
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 8 cut(s) 317, 509, 569, 581, 826, 972, 1090, 1129
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 2 cut(s) 318, 576
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 3 cut(s) 94, 758, 1013
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 3 cut(s) 171, 301, 624
BveI ACCTGC 2 cut(s) 208, 657
Cac8I GCNNGC 5 cut(s) 511, 654, 745, 1092, 1131
CciI TCATGA 2 cut(s) 1111, 1150
CfoI GCGC 2 cut(s) 597, 844
Cfr10I RCCGGY 1 cut(s) 822
Cfr13I GGNCC 4 cut(s) 431, 767, 1088, 1127
Cfr42I CCGCGG 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 483
CseI GACGC 1 cut(s) 1041
Csp6I GTAC 5 cut(s) 37, 263, 472, 691, 946
CviAII CATG 9 cut(s) 358, 381, 577, 880, 974, 988, 1112, 1145, 1151
CviQI GTAC 5 cut(s) 37, 263, 472, 691, 946
DdeI CTNAG 3 cut(s) 156, 184, 255
DinI GGCGCC 1 cut(s) 843
DpnI GATC 5 cut(s) 303, 1110, 1116, 1149, 1155
DpnII GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
DrdI GACNNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 2 cut(s) 579, 970
EciI GGCGGA 2 cut(s) 42, 338
Eco130I CCWWGG 2 cut(s) 312, 576
Eco47I GGWCC 2 cut(s) 431, 767
Eco57I CTGAAG 1 cut(s) 162
EcoO109I RGGNCCY 2 cut(s) 1088, 1127
EcoRII CCWGG 1 cut(s) 554
EcoT14I CCWWGG 2 cut(s) 312, 576
EgeI GGCGCC 1 cut(s) 843
EheI GGCGCC 1 cut(s) 843
ErhI CCWWGG 2 cut(s) 312, 576
FaeI CATG 9 cut(s) 361, 384, 580, 883, 977, 991, 1115, 1148, 1154
FaqI GGGAC 3 cut(s) 359, 375, 625
FatI CATG 9 cut(s) 357, 380, 576, 879, 973, 987, 1111, 1144, 1150
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 1081
FbaI TGATCA 4 cut(s) 1108, 1114, 1147, 1153
Fnu4HI GCNGC 9 cut(s) 318, 321, 405, 630, 817, 1095, 1098, 1134, 1137
FokI GGATG 3 cut(s) 81, 745, 1000
Fsp4HI GCNGC 9 cut(s) 318, 321, 405, 630, 817, 1095, 1098, 1134, 1137
FspBI CTAG 1 cut(s) 1174
GlaI GCGC 2 cut(s) 596, 843
GluI GCNGC 9 cut(s) 318, 321, 405, 630, 817, 1095, 1098, 1134, 1137
GsuI CTGGAG 4 cut(s) 452, 499, 577, 935
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 8 cut(s) 317, 509, 569, 581, 826, 972, 1090, 1129
HapII CCGG 6 cut(s) 389, 434, 765, 777, 823, 849
HgaI GACGC 1 cut(s) 1041
HhaI GCGC 2 cut(s) 597, 844
Hin1I GRCGYC 1 cut(s) 842
Hin1II CATG 9 cut(s) 361, 384, 580, 883, 977, 991, 1115, 1148, 1154
Hin6I GCGC 2 cut(s) 595, 842
HinP1I GCGC 2 cut(s) 595, 842
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 2 cut(s) 20, 572
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 6 cut(s) 389, 434, 765, 777, 823, 849
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 159, 256
Hpy188III TCNNGA 7 cut(s) 230, 434, 624, 952, 1056, 1112, 1151
Hpy8I GTNNAC 1 cut(s) 421
Hpy99I CGWCG 1 cut(s) 940
HpyAV CCTTC 4 cut(s) 855, 1079, 1117, 1156
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 4 cut(s) 239, 652, 816, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 317, 326, 635, 899
HpyF3I CTNAG 3 cut(s) 156, 184, 255
HpySE526I ACGT 1 cut(s) 354
Hsp92I GRCGYC 1 cut(s) 842
Hsp92II CATG 9 cut(s) 361, 384, 580, 883, 977, 991, 1115, 1148, 1154
HspAI GCGC 2 cut(s) 595, 842
KasI GGCGCC 1 cut(s) 841
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 4 cut(s) 1108, 1114, 1147, 1153
KspAI GTTAAC 1 cut(s) 421
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
LmnI GCTCC 6 cut(s) 208, 518, 558, 626, 732, 864
Lsp1109I GCAGC 7 cut(s) 416, 641, 828, 1081, 1084, 1120, 1123
LweI GCATC 3 cut(s) 248, 1022, 1030
MaeI CTAG 1 cut(s) 1174
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 2 cut(s) 856, 1169
MalI GATC 5 cut(s) 303, 1110, 1116, 1149, 1155
MboI GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
MboII GAAGA 5 cut(s) 125, 240, 311, 538, 652
MflI RGATCY 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 1 cut(s) 1000
MluNI TGGCCA 1 cut(s) 581
Mly113I GGCGCC 1 cut(s) 842
MlyI GAGTC 1 cut(s) 581
Mox20I TGGCCA 1 cut(s) 581
MroI TCCGGA 1 cut(s) 433
MscI TGGCCA 1 cut(s) 581
MseI TTAA 4 cut(s) 420, 603, 665, 999
MslI CAYNNNNRTG 1 cut(s) 1149
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 6 cut(s) 389, 434, 765, 777, 823, 849
MspR9I CCNGG 2 cut(s) 389, 556
MvaI CCWGG 1 cut(s) 556
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 5 cut(s) 143, 317, 326, 635, 899
NarI GGCGCC 1 cut(s) 842
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 1081
NdeII GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
NlaIII CATG 9 cut(s) 361, 384, 580, 883, 977, 991, 1115, 1148, 1154
NlaIV GGNNCC 4 cut(s) 560, 843, 885, 1128
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 2 cut(s) 856, 1169
NspI RCATGY 1 cut(s) 384
PagI TCATGA 2 cut(s) 1111, 1150
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PfeI GAWTC 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 554
PkrI GCNGC 9 cut(s) 319, 322, 406, 631, 818, 1096, 1099, 1135, 1138
PleI GAGTC 1 cut(s) 580
PluTI GGCGCC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 554
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 4 cut(s) 560, 843, 885, 1128
PspPI GGNCC 4 cut(s) 431, 767, 1088, 1127
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 5 cut(s) 38, 264, 473, 692, 947
RsaNI GTAC 5 cut(s) 37, 263, 472, 691, 946
RseI CAYNNNNRTG 1 cut(s) 1149
SacII CCGCGG 1 cut(s) 321
SaqAI TTAA 4 cut(s) 420, 603, 665, 999
SatI GCNGC 9 cut(s) 318, 321, 405, 630, 817, 1095, 1098, 1134, 1137
Sau3AI GATC 5 cut(s) 301, 1108, 1114, 1147, 1153
Sau96I GGNCC 4 cut(s) 431, 767, 1088, 1127
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 389, 556
SfaNI GCATC 3 cut(s) 248, 1022, 1030
SfoI GGCGCC 1 cut(s) 843
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 2 cut(s) 431, 767
SmiMI CAYNNNNRTG 1 cut(s) 1149
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 1 cut(s) 1000
SsiI CCGC 6 cut(s) 53, 105, 318, 320, 323, 807
SspDI GGCGCC 1 cut(s) 841
SspI AATATT 1 cut(s) 799
SspMI CTAG 1 cut(s) 1174
StyD4I CCNGG 2 cut(s) 387, 554
StyI CCWWGG 2 cut(s) 312, 576
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 483
TasI AATT 1 cut(s) 1000
TatI WGTACW 1 cut(s) 690
TauI GCSGC 2 cut(s) 320, 323
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 4 cut(s) 420, 603, 665, 999
Tru9I TTAA 4 cut(s) 420, 603, 665, 999
TscAI CASTG 3 cut(s) 178, 301, 624
TseFI GTSAC 2 cut(s) 856, 1169
TseI GCWGC 7 cut(s) 404, 629, 816, 1094, 1097, 1133, 1136
Tsp45I GTSAC 2 cut(s) 856, 1169
TspDTI ATGAA 7 cut(s) 17, 74, 240, 659, 976, 1020, 1098
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 3 cut(s) 178, 301, 624
VpaK11BI GGWCC 2 cut(s) 431, 767
XceI RCATGY 1 cut(s) 384
XspI CTAG 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.