Rmu_sc0000405.1_g000025

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000405.1
OG0004816
Physical Location & Seq
Reverse (-)
118826 .. 120074
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000405.1_g000025.1.cds

Sequence Viewer

Length: 1146 bp
atgaacaacagcaaccaagaatctcaaccacaaccacctatgtacccctccattcctcctcctctcacagcacacgaagcaaacccttttcttcaatctcatccagaagatatcaatccattttctcaaaccacttccccaccagaagccccaaaacctcaaaccccctccattactgccgaccacttagaaaaatggcaacctgctcctcatgatcatcatcatgatcatcatcatcaagatattccatcatcttcatctgagcaagtaccagtagcagcaccaccacccacctttgacagcgaagatctcaaagccaaggccgcggcggaggcagagcctgagaaatggggcactcacgtcatgggacacccggctgtcccaacttgccacccggacaacaaaaaggcagctctatggggagtagccgccgatagtacagagaaagctcagagcttccactatccgtacttccagtaccaacccattgatcacaacaacaaggccagcagctccagcaatcccactcaatctatgctcaatgtcttcaactcctggagccacaaagccgagtccatggcaaacaacatctggcacaaccttaaaacagggacatctgtgtccggagcagcatgggcgaagatgaacctgcaggcgaaagcattaacaggaggtggatttgaggctatctacaagcagacatttgcgacttatcccaacgagaagctcctcaaatcattcgcctgttatctctccacatccaccggaccagtcgccggaactctttacctgtccaatattcacacagctttttgcagcgaccggccattgtcattcactgcaccttccggtcaggtgacttggagctattacaaggttatggtgccgctggcaaatgttgcgactatcaatccagtggtgatgagggagaatccgactgagaagtacctccagattgtgaccattgacggccatgatttctggttcatggggtttgttaattatgaaaaagcatccaaacatcttactcagagtatctccactttcgtagcgtctggatttgcagtccaacctcccccatatgaaaagcatgctggtgctgaacatgatcatgatcatgatcacaagaagggaggtgacaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

381

Amino Acids

42.0

Weight (kDa)

6.18

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 619
Acc36I ACCTGC 2 cut(s) 211, 657
AccB1I GGYRCC 1 cut(s) 883
AccII CGCG 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 623
AciI CCGC 5 cut(s) 324, 326, 329, 429, 887
AcoI YGGCCR 2 cut(s) 824, 970
AfaI GTAC 6 cut(s) 44, 270, 439, 470, 479, 947
AfiI CCNNNNNNNGG 1 cut(s) 776
AgsI TTSAA 2 cut(s) 95, 550
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 554
AluBI AGCT 7 cut(s) 413, 449, 456, 513, 727, 809, 867
AluI AGCT 7 cut(s) 413, 449, 456, 513, 727, 809, 867
AlwNI CAGNNNCTG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 623
AoxI GGCC 4 cut(s) 321, 504, 824, 970
ApeKI GCWGC 5 cut(s) 278, 410, 510, 629, 816
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 2 cut(s) 374, 395
AsuHPI GGTGA 2 cut(s) 868, 931
AvaII GGWCC 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 356
BaeI ACNNNNGTAYC 2 cut(s) 26, 59
BanI GGYRCC 1 cut(s) 883
BbsI GAAGAC 1 cut(s) 538
BbvI GCAGC 5 cut(s) 290, 422, 522, 641, 828
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 985
BciT130I CCWGG 1 cut(s) 556
BclI TGATCA 6 cut(s) 214, 226, 490, 1108, 1114, 1120
BcnI CCSGG 2 cut(s) 374, 395
BfmI CTRYAG 1 cut(s) 650
BfuAI ACCTGC 2 cut(s) 211, 657
BglII AGATCT 1 cut(s) 307
BisI GCNGC 9 cut(s) 279, 324, 327, 411, 429, 511, 630, 817, 887
BlsI GCNGC 9 cut(s) 280, 325, 328, 412, 430, 512, 631, 818, 888
Bme1390I CCNGG 3 cut(s) 374, 395, 556
Bme18I GGWCC 1 cut(s) 767
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 2 cut(s) 560, 885
BmrFI CCNGG 3 cut(s) 374, 395, 556
BmsI GCATC 1 cut(s) 1022
BpiI GAAGAC 1 cut(s) 538
BpmI CTGGAG 3 cut(s) 499, 577, 935
BpuMI CCSGG 2 cut(s) 374, 395
BsaBI GATNNNNATC 5 cut(s) 114, 219, 231, 1113, 1119
BsaJI CCNNGG 3 cut(s) 318, 324, 576
BsaWI WCCGGW 3 cut(s) 623, 764, 848
Bsc4I CCNNNNNNNGG 1 cut(s) 776
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 4 cut(s) 272, 475, 770, 914
Bse8I GATNNNNATC 5 cut(s) 114, 219, 231, 1113, 1119
BseAI TCCGGA 1 cut(s) 623
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 3 cut(s) 318, 324, 576
BseGI GGATG 3 cut(s) 100, 758, 1013
BseJI GATNNNNATC 5 cut(s) 114, 219, 231, 1113, 1119
BseLI CCNNNNNNNGG 1 cut(s) 776
BseMII CTCAG 5 cut(s) 252, 333, 464, 930, 1043
BseNI ACTGG 4 cut(s) 272, 475, 770, 914
BseRI GAGGAG 4 cut(s) 48, 51, 198, 719
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 5 cut(s) 290, 422, 522, 641, 828
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 823
BshFI GGCC 4 cut(s) 323, 506, 826, 972
BshNI GGYRCC 1 cut(s) 883
BsiEI CGRYCG 1 cut(s) 823
BsiSI CCGG 7 cut(s) 374, 395, 624, 765, 777, 823, 849
BslFI GGGAC 3 cut(s) 365, 381, 625
BslI CCNNNNNNNGG 1 cut(s) 776
BsmFI GGGAC 3 cut(s) 365, 381, 625
BsnI GGCC 4 cut(s) 323, 506, 826, 972
Bsp1286I GDGCHC 1 cut(s) 356
Bsp13I TCCGGA 1 cut(s) 623
Bsp143I GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 5 cut(s) 324, 326, 329, 429, 887
BspANI GGCC 4 cut(s) 323, 506, 826, 972
BspCNI CTCAG 5 cut(s) 253, 334, 463, 931, 1042
BspEI TCCGGA 1 cut(s) 623
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 4 cut(s) 211, 223, 1111, 1117
BspLI GGNNCC 2 cut(s) 560, 885
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 2 cut(s) 211, 657
BspT107I GGYRCC 1 cut(s) 883
BsrFI RCCGGY 1 cut(s) 822
BsrI ACTGG 4 cut(s) 272, 475, 770, 914
BssAI RCCGGY 1 cut(s) 822
BssECI CCNNGG 3 cut(s) 318, 324, 576
BssMI GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
BssT1I CCWWGG 2 cut(s) 318, 576
Bst2UI CCWGG 1 cut(s) 556
BstAPI GCANNNNNTGC 1 cut(s) 899
BstC8I GCNNGC 4 cut(s) 508, 654, 891, 1092
BstDEI CTNAG 6 cut(s) 187, 261, 342, 450, 939, 1029
BstDSI CCRYGG 2 cut(s) 324, 576
BstF5I GGATG 3 cut(s) 100, 758, 1013
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 7 cut(s) 217, 229, 310, 493, 1111, 1117, 1123
BstMBI GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
BstMCI CGRYCG 1 cut(s) 823
BstMWI GCNNNNNNNGC 6 cut(s) 77, 323, 332, 516, 635, 899
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 1 cut(s) 1094
BstSCI CCNGG 3 cut(s) 372, 393, 554
BstSFI CTRYAG 1 cut(s) 650
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 5 cut(s) 290, 422, 522, 641, 828
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 4 cut(s) 323, 506, 826, 972
BtgI CCRYGG 2 cut(s) 324, 576
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 3 cut(s) 100, 758, 1013
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 2 cut(s) 837, 921
BveI ACCTGC 2 cut(s) 211, 657
Cac8I GCNNGC 4 cut(s) 508, 654, 891, 1092
CaiI CAGNNNCTG 1 cut(s) 341
CciI TCATGA 4 cut(s) 211, 223, 1111, 1117
Cfr10I RCCGGY 1 cut(s) 822
Cfr13I GGNCC 1 cut(s) 767
Cfr42I CCGCGG 1 cut(s) 327
CseI GACGC 1 cut(s) 1041
Csp6I GTAC 6 cut(s) 43, 269, 438, 469, 478, 946
CviQI GTAC 6 cut(s) 43, 269, 438, 469, 478, 946
DdeI CTNAG 6 cut(s) 187, 261, 342, 450, 939, 1029
DpnI GATC 7 cut(s) 216, 228, 309, 492, 1110, 1116, 1122
DpnII GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
DrdI GACNNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 2 cut(s) 824, 970
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 318, 576
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 767
EcoRII CCWGG 1 cut(s) 554
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 318, 576
ErhI CCWWGG 2 cut(s) 318, 576
FaqI GGGAC 3 cut(s) 365, 381, 625
FauNDI CATATG 1 cut(s) 1081
FbaI TGATCA 6 cut(s) 214, 226, 490, 1108, 1114, 1120
Fnu4HI GCNGC 9 cut(s) 279, 324, 327, 411, 429, 511, 630, 817, 887
FokI GGATG 3 cut(s) 87, 745, 1000
Fsp4HI GCNGC 9 cut(s) 279, 324, 327, 411, 429, 511, 630, 817, 887
GluI GCNGC 9 cut(s) 279, 324, 327, 411, 429, 511, 630, 817, 887
GsuI CTGGAG 3 cut(s) 499, 577, 935
HaeIII GGCC 4 cut(s) 323, 506, 826, 972
HapII CCGG 7 cut(s) 374, 395, 624, 765, 777, 823, 849
HgaI GACGC 1 cut(s) 1041
HinfI GANTC 3 cut(s) 20, 572, 931
HpaII CCGG 7 cut(s) 374, 395, 624, 765, 777, 823, 849
HphI GGTGA 2 cut(s) 868, 931
Hpy188I TCNGA 4 cut(s) 262, 453, 936, 1032
Hpy188III TCNNGA 9 cut(s) 104, 212, 224, 239, 624, 952, 1056, 1112, 1118
HpyAV CCTTC 2 cut(s) 855, 1123
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 4 cut(s) 652, 816, 842, 1064
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 323, 332, 516, 635, 899
HpyF3I CTNAG 6 cut(s) 187, 261, 342, 450, 939, 1029
HpySE526I ACGT 1 cut(s) 360
Kpn2I TCCGGA 1 cut(s) 623
Ksp22I TGATCA 6 cut(s) 214, 226, 490, 1108, 1114, 1120
KspI CCGCGG 1 cut(s) 327
Kzo9I GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
LmnI GCTCC 6 cut(s) 211, 518, 558, 626, 732, 864
Lsp1109I GCAGC 5 cut(s) 290, 422, 522, 641, 828
LweI GCATC 1 cut(s) 1022
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 3 cut(s) 856, 958, 1136
MalI GATC 7 cut(s) 216, 228, 309, 492, 1110, 1116, 1122
MboI GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
MboII GAAGA 6 cut(s) 83, 119, 246, 317, 538, 652
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 1 cut(s) 1000
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 2 cut(s) 959, 1093
MroI TCCGGA 1 cut(s) 623
MseI TTAA 3 cut(s) 603, 665, 999
MslI CAYNNNNRTG 4 cut(s) 222, 1095, 1110, 1116
MspA1I CMGCKG 2 cut(s) 326, 889
MspI CCGG 7 cut(s) 374, 395, 624, 765, 777, 823, 849
MspR9I CCNGG 3 cut(s) 374, 395, 556
MvaI CCWGG 1 cut(s) 556
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 6 cut(s) 77, 323, 332, 516, 635, 899
NciI CCSGG 2 cut(s) 374, 395
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 1081
NdeII GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
NlaIV GGNNCC 2 cut(s) 560, 885
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 3 cut(s) 856, 958, 1136
NspI RCATGY 1 cut(s) 1094
PaeI GCATGC 1 cut(s) 1094
PagI TCATGA 4 cut(s) 211, 223, 1111, 1117
PfeI GAWTC 2 cut(s) 20, 931
PfoI TCCNGGA 1 cut(s) 554
PkrI GCNGC 9 cut(s) 280, 325, 328, 412, 430, 512, 631, 818, 888
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 554
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 560, 885
PspPI GGNCC 1 cut(s) 767
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 6 cut(s) 44, 270, 439, 470, 479, 947
RsaNI GTAC 6 cut(s) 43, 269, 438, 469, 478, 946
RseI CAYNNNNRTG 4 cut(s) 222, 1095, 1110, 1116
SacII CCGCGG 1 cut(s) 327
SaqAI TTAA 3 cut(s) 603, 665, 999
SatI GCNGC 9 cut(s) 279, 324, 327, 411, 429, 511, 630, 817, 887
Sau3AI GATC 7 cut(s) 214, 226, 307, 490, 1108, 1114, 1120
Sau96I GGNCC 1 cut(s) 767
SbfI CCTGCAGG 1 cut(s) 654
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 3 cut(s) 374, 395, 556
SdaI CCTGCAGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 356
SfaNI GCATC 1 cut(s) 1022
SfcI CTRYAG 1 cut(s) 650
Sfr303I CCGCGG 1 cut(s) 327
SgrBI CCGCGG 1 cut(s) 327
SinI GGWCC 1 cut(s) 767
SmiMI CAYNNNNRTG 4 cut(s) 222, 1095, 1110, 1116
SphI GCATGC 1 cut(s) 1094
Sse8387I CCTGCAGG 1 cut(s) 654
Sse9I AATT 1 cut(s) 1000
SsiI CCGC 5 cut(s) 324, 326, 329, 429, 887
SspI AATATT 1 cut(s) 799
StyD4I CCNGG 3 cut(s) 372, 393, 554
StyI CCWWGG 2 cut(s) 318, 576
TaiI ACGT 1 cut(s) 363
TasI AATT 1 cut(s) 1000
TatI WGTACW 1 cut(s) 437
TauI GCSGC 4 cut(s) 326, 329, 431, 889
TfiI GAWTC 2 cut(s) 20, 931
Tru1I TTAA 3 cut(s) 603, 665, 999
Tru9I TTAA 3 cut(s) 603, 665, 999
TscAI CASTG 2 cut(s) 844, 921
TseFI GTSAC 3 cut(s) 856, 958, 1136
TseI GCWGC 5 cut(s) 278, 410, 510, 629, 816
Tsp45I GTSAC 3 cut(s) 856, 958, 1136
TspDTI ATGAA 6 cut(s) 17, 246, 659, 976, 1020, 1098
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 844, 921
VpaK11BI GGWCC 1 cut(s) 767
XceI RCATGY 1 cut(s) 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.