Rh2DG696200

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004816
Physical Location & Seq
Reverse (-)
91304257 .. 91306375
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG696200.1

Sequence Viewer

Length: 1137 bp
ATGAACAACAGCAACCAAGAATCTCAACCACAACCACCTATGTACCCCTCCATTCCTCCTCCTCTCACAGCACACGAAGCAAACCCTTTTCTTCAATCTCATCCAGAAGATATCAATCCATTTTCTCAAACCACTTCCCCACCAGAAGCCCCAAAACCTCAAACCCCCTCCATTACTGCCGACCACTTAGAAAAATGGCAACCTGCTCCTCATGATCATGATCATCATCAAGATATTCCATCATCTTCATCTGAGCAAGTACCAGTAGCAGCACCACCACCCACCTTTGACAGCGAAGATCTCAAAGCCAAGGCCGCGGCGGAGGCAGAGCCTGAGAAATGGGGCACTCACGTCATGGGACACCCGGCTGTCCCAACTTGCCACCCGGACAACAAAAAGGCCGCTCTATGGGGAGTAGCCGCCGATAGTACAGAAAAAGCTCAGAGCTTCCACTATCCGTACCTCCAGTACCAACCCATTGATCACAACAACAAGGCCAGCAGCTCCAGCAATCCCACTCAATCTATGCTCAATGTCTTCAACTCCTGGAGCCACAAAGCCGAGTCCATGGCAAACAACATCTGGCACAACCTTAAAACAGGGACATCTGTGTCCGGAGCAGCATGGGCGAAGATGAACCTGCAGGCGAAAGCATTAACAGGAGGTGGATTTGAGGCTATCTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTCCTCAAATCATTCGCCTGTTATCTCTCCACATCCACCGGACCAGTCGCCGGAACTCTTTACCTGTCCAATATTCACACAGCTTTTTGCAGCGACCGGCCATTGTCATTCACTGCACCTTCCGGTCAGGTGACTTGGAGCTATTACAAGGTTATGGTGCCTCTGGCAAATGTTGCGACTATCAATCCAGTGGTGATGAGGGAGAATCCGACTGAGAAGTACCTCCAGATTGTGACCATTGACGGCCATGATTTCTGGTTCATGGGGTTTGTTAATTATGAAAAAGCATCCAAACATCTTACTCAGAGTATCTCCACTTTCGTAGCGTCTGGATTTGCAGTCCAACCTCCCCCATATGAAAAGCATGCTGGTGCTGAACATGATCATGATCATGATCACAAGAAGGGAGGTGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

378

Amino Acids

41.54

Weight (kDa)

6.03

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 229 - 341 7.8e-14 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 610
Acc36I ACCTGC 2 cut(s) 211, 648
AccB1I GGYRCC 1 cut(s) 874
AccBSI CCGCTC 1 cut(s) 404
AccII CGCG 1 cut(s) 317
AccIII TCCGGA 1 cut(s) 614
AciI CCGC 5 cut(s) 315, 317, 320, 402, 420
AcoI YGGCCR 2 cut(s) 815, 961
AfaI GTAC 6 cut(s) 44, 261, 430, 461, 470, 938
AfiI CCNNNNNNNGG 2 cut(s) 408, 767
AgsI TTSAA 2 cut(s) 95, 541
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 1 cut(s) 545
AluBI AGCT 6 cut(s) 440, 447, 504, 718, 800, 858
AluI AGCT 6 cut(s) 440, 447, 504, 718, 800, 858
AlwNI CAGNNNCTG 1 cut(s) 332
Aor13HI TCCGGA 1 cut(s) 614
AoxI GGCC 5 cut(s) 312, 399, 495, 815, 961
ApeKI GCWGC 4 cut(s) 269, 501, 620, 807
AspS9I GGNCC 1 cut(s) 758
AsuC2I CCSGG 2 cut(s) 365, 386
AsuHPI GGTGA 2 cut(s) 859, 922
AvaII GGWCC 1 cut(s) 758
BaeGI GKGCMC 1 cut(s) 347
BaeI ACNNNNGTAYC 2 cut(s) 26, 59
BanI GGYRCC 1 cut(s) 874
BbsI GAAGAC 1 cut(s) 529
BbvI GCAGC 4 cut(s) 281, 513, 632, 819
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 976
BciT130I CCWGG 1 cut(s) 547
BclI TGATCA 6 cut(s) 214, 220, 481, 1099, 1105, 1111
BcnI CCSGG 2 cut(s) 365, 386
BfmI CTRYAG 1 cut(s) 641
BfuAI ACCTGC 2 cut(s) 211, 648
BglII AGATCT 1 cut(s) 298
BisI GCNGC 8 cut(s) 270, 315, 318, 402, 420, 502, 621, 808
BlsI GCNGC 8 cut(s) 271, 316, 319, 403, 421, 503, 622, 809
Bme1390I CCNGG 3 cut(s) 365, 386, 547
Bme18I GGWCC 1 cut(s) 758
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 2 cut(s) 551, 876
BmrFI CCNGG 3 cut(s) 365, 386, 547
BmsI GCATC 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 4 cut(s) 449, 490, 568, 926
BpuMI CCSGG 2 cut(s) 365, 386
BsaBI GATNNNNATC 5 cut(s) 114, 219, 225, 1104, 1110
BsaJI CCNNGG 3 cut(s) 309, 315, 567
BsaWI WCCGGW 3 cut(s) 614, 755, 839
Bsc4I CCNNNNNNNGG 2 cut(s) 408, 767
Bse118I RCCGGY 1 cut(s) 813
Bse1I ACTGG 4 cut(s) 263, 466, 761, 905
Bse8I GATNNNNATC 5 cut(s) 114, 219, 225, 1104, 1110
BseAI TCCGGA 1 cut(s) 614
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 309, 315, 567
BseGI GGATG 3 cut(s) 100, 749, 1004
BseJI GATNNNNATC 5 cut(s) 114, 219, 225, 1104, 1110
BseLI CCNNNNNNNGG 2 cut(s) 408, 767
BseMII CTCAG 5 cut(s) 243, 324, 455, 921, 1034
BseNI ACTGG 4 cut(s) 263, 466, 761, 905
BseRI GAGGAG 4 cut(s) 48, 51, 198, 710
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 4 cut(s) 281, 513, 632, 819
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 5 cut(s) 314, 401, 497, 817, 963
BshNI GGYRCC 1 cut(s) 874
BsiEI CGRYCG 1 cut(s) 814
BsiSI CCGG 7 cut(s) 365, 386, 615, 756, 768, 814, 840
BslFI GGGAC 3 cut(s) 356, 372, 616
BslI CCNNNNNNNGG 2 cut(s) 408, 767
BsmFI GGGAC 3 cut(s) 356, 372, 616
BsnI GGCC 5 cut(s) 314, 401, 497, 817, 963
Bsp1286I GDGCHC 1 cut(s) 347
Bsp13I TCCGGA 1 cut(s) 614
Bsp143I GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
Bsp19I CCATGG 1 cut(s) 567
BspACI CCGC 5 cut(s) 315, 317, 320, 402, 420
BspANI GGCC 5 cut(s) 314, 401, 497, 817, 963
BspCNI CTCAG 5 cut(s) 244, 325, 454, 922, 1033
BspEI TCCGGA 1 cut(s) 614
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 4 cut(s) 211, 217, 1102, 1108
BspLI GGNNCC 2 cut(s) 551, 876
BspMAI CTGCAG 1 cut(s) 645
BspMI ACCTGC 2 cut(s) 211, 648
BspT107I GGYRCC 1 cut(s) 874
BsrBI CCGCTC 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 813
BsrI ACTGG 4 cut(s) 263, 466, 761, 905
BssAI RCCGGY 1 cut(s) 813
BssECI CCNNGG 3 cut(s) 309, 315, 567
BssMI GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
BssT1I CCWWGG 2 cut(s) 309, 567
Bst2UI CCWGG 1 cut(s) 547
BstAPI GCANNNNNTGC 1 cut(s) 890
BstC8I GCNNGC 3 cut(s) 499, 645, 1083
BstDEI CTNAG 6 cut(s) 187, 252, 333, 441, 930, 1020
BstDSI CCRYGG 2 cut(s) 315, 567
BstF5I GGATG 3 cut(s) 100, 749, 1004
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 7 cut(s) 217, 223, 301, 484, 1102, 1108, 1114
BstMBI GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 6 cut(s) 77, 314, 323, 507, 626, 890
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 1 cut(s) 1085
BstSCI CCNGG 3 cut(s) 363, 384, 545
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 1 cut(s) 347
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 4 cut(s) 281, 513, 632, 819
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 5 cut(s) 314, 401, 497, 817, 963
BtgI CCRYGG 2 cut(s) 315, 567
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 3 cut(s) 100, 749, 1004
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 828, 912
BveI ACCTGC 2 cut(s) 211, 648
Cac8I GCNNGC 3 cut(s) 499, 645, 1083
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 4 cut(s) 211, 217, 1102, 1108
Cfr10I RCCGGY 1 cut(s) 813
Cfr13I GGNCC 1 cut(s) 758
Cfr42I CCGCGG 1 cut(s) 318
CseI GACGC 1 cut(s) 1032
Csp6I GTAC 6 cut(s) 43, 260, 429, 460, 469, 937
CviQI GTAC 6 cut(s) 43, 260, 429, 460, 469, 937
DdeI CTNAG 6 cut(s) 187, 252, 333, 441, 930, 1020
DpnI GATC 7 cut(s) 216, 222, 300, 483, 1101, 1107, 1113
DpnII GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
DrdI GACNNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 2 cut(s) 815, 961
EciI GGCGGA 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 309, 567
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 758
EcoRII CCWGG 1 cut(s) 545
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 309, 567
ErhI CCWWGG 2 cut(s) 309, 567
FaqI GGGAC 3 cut(s) 356, 372, 616
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 6 cut(s) 214, 220, 481, 1099, 1105, 1111
Fnu4HI GCNGC 8 cut(s) 270, 315, 318, 402, 420, 502, 621, 808
FokI GGATG 3 cut(s) 87, 736, 991
Fsp4HI GCNGC 8 cut(s) 270, 315, 318, 402, 420, 502, 621, 808
GluI GCNGC 8 cut(s) 270, 315, 318, 402, 420, 502, 621, 808
GsuI CTGGAG 4 cut(s) 449, 490, 568, 926
HaeIII GGCC 5 cut(s) 314, 401, 497, 817, 963
HapII CCGG 7 cut(s) 365, 386, 615, 756, 768, 814, 840
HgaI GACGC 1 cut(s) 1032
HinfI GANTC 3 cut(s) 20, 563, 922
HpaII CCGG 7 cut(s) 365, 386, 615, 756, 768, 814, 840
HphI GGTGA 2 cut(s) 859, 922
Hpy188I TCNGA 4 cut(s) 253, 444, 927, 1023
Hpy188III TCNNGA 9 cut(s) 104, 212, 218, 230, 615, 943, 1047, 1103, 1109
HpyAV CCTTC 2 cut(s) 846, 1114
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 643, 807, 833, 1055
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 314, 323, 507, 626, 890
HpyF3I CTNAG 6 cut(s) 187, 252, 333, 441, 930, 1020
HpySE526I ACGT 1 cut(s) 351
Kpn2I TCCGGA 1 cut(s) 614
Ksp22I TGATCA 6 cut(s) 214, 220, 481, 1099, 1105, 1111
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
LmnI GCTCC 6 cut(s) 211, 509, 549, 617, 723, 855
Lsp1109I GCAGC 4 cut(s) 281, 513, 632, 819
LweI GCATC 1 cut(s) 1013
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 3 cut(s) 847, 949, 1127
MalI GATC 7 cut(s) 216, 222, 300, 483, 1101, 1107, 1113
MbiI CCGCTC 1 cut(s) 404
MboI GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
MboII GAAGA 6 cut(s) 83, 119, 237, 308, 529, 643
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 2 cut(s) 991, 1132
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 2 cut(s) 950, 1084
MroI TCCGGA 1 cut(s) 614
MseI TTAA 3 cut(s) 594, 656, 990
MslI CAYNNNNRTG 4 cut(s) 216, 1086, 1101, 1107
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 7 cut(s) 365, 386, 615, 756, 768, 814, 840
MspR9I CCNGG 3 cut(s) 365, 386, 547
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 77, 314, 323, 507, 626, 890
NciI CCSGG 2 cut(s) 365, 386
NcoI CCATGG 1 cut(s) 567
NdeI CATATG 1 cut(s) 1072
NdeII GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
NlaIV GGNNCC 2 cut(s) 551, 876
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 3 cut(s) 847, 949, 1127
NspI RCATGY 1 cut(s) 1085
PaeI GCATGC 1 cut(s) 1085
PagI TCATGA 4 cut(s) 211, 217, 1102, 1108
PfeI GAWTC 2 cut(s) 20, 922
PfoI TCCNGGA 1 cut(s) 545
PkrI GCNGC 8 cut(s) 271, 316, 319, 403, 421, 503, 622, 809
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 2 cut(s) 551, 876
PspPI GGNCC 1 cut(s) 758
PstI CTGCAG 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 6 cut(s) 44, 261, 430, 461, 470, 938
RsaNI GTAC 6 cut(s) 43, 260, 429, 460, 469, 937
RseI CAYNNNNRTG 4 cut(s) 216, 1086, 1101, 1107
SacII CCGCGG 1 cut(s) 318
SaqAI TTAA 3 cut(s) 594, 656, 990
SatI GCNGC 8 cut(s) 270, 315, 318, 402, 420, 502, 621, 808
Sau3AI GATC 7 cut(s) 214, 220, 298, 481, 1099, 1105, 1111
Sau96I GGNCC 1 cut(s) 758
SbfI CCTGCAGG 1 cut(s) 645
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 365, 386, 547
SdaI CCTGCAGG 1 cut(s) 645
SduI GDGCHC 1 cut(s) 347
SfaNI GCATC 1 cut(s) 1013
SfcI CTRYAG 1 cut(s) 641
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 4 cut(s) 216, 1086, 1101, 1107
SphI GCATGC 1 cut(s) 1085
Sse8387I CCTGCAGG 1 cut(s) 645
Sse9I AATT 2 cut(s) 991, 1132
SsiI CCGC 5 cut(s) 315, 317, 320, 402, 420
SspI AATATT 1 cut(s) 790
StyD4I CCNGG 3 cut(s) 363, 384, 545
StyI CCWWGG 2 cut(s) 309, 567
TaiI ACGT 1 cut(s) 354
TasI AATT 2 cut(s) 991, 1132
TatI WGTACW 1 cut(s) 428
TauI GCSGC 4 cut(s) 317, 320, 404, 422
TfiI GAWTC 2 cut(s) 20, 922
Tru1I TTAA 3 cut(s) 594, 656, 990
Tru9I TTAA 3 cut(s) 594, 656, 990
TscAI CASTG 2 cut(s) 835, 912
TseFI GTSAC 3 cut(s) 847, 949, 1127
TseI GCWGC 4 cut(s) 269, 501, 620, 807
Tsp45I GTSAC 3 cut(s) 847, 949, 1127
TspDTI ATGAA 6 cut(s) 17, 237, 650, 967, 1011, 1089
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 2 cut(s) 835, 912
VpaK11BI GGWCC 1 cut(s) 758
XceI RCATGY 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.