Rh2BG682800

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004816
Physical Location & Seq
Reverse (-)
89158506 .. 89160623
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG682800.1

Sequence Viewer

Length: 1143 bp
ATGAACAACAGCAACCAAGAGTCTCAACCACAACCACCTATGTACCCCTCCATTCCTCCCCCTCTCACAGCACACGAAGCAAACCCTTTTCTTCAATCTCATCCAGAAGATATCAATCCATTTTCTCAAACCACTTCCCCACCAGAAGCCCCAAAACCTCAAACCCCCTCCATTACTGCCGACCAATTAGAAAAATGGCAACCTGCTCCTCCTGATCATGATCATCATCATCAAGATATTCCATCATCTTCATCTGAGCAAGTACCAGTAGCAGCACCACCACCCACCTTTGACAGCGAAGATCTCAAAGCCAAGGCCGCGGCGGAGGCAGAGCCTGAGAAATGGGGCACTCACGTCATGGGACACCCGGCTGTCCCAACTTGCCACCCGGACAACAAAAAGGCAGCTCTATGGGGAGTAGCCGCCGATAGTACAGAAAAAGCTCAGAGCTTCCACTATCCGTACCTCCAGTACCAACCCATTGATCACAACAACAAGCAGGCCAGCAGCTCCAGCAATCCCACTCAATCTATGCTCAATGTCTTCAACTCCTGGAGCCACAAAGCCGAGTCCATGGCCAACAACATCTGGCACAACCTTAAAACAGGGACATCTGTGTCCGGAGCAGCGTGGGCGAAGATGAACCTGCAGGCGAAAGCATTAACAGGAGGTGGATTTGAGGCTATCTACAAGCAGACATTTGCGACTTATCCCAACGAGAATCTCCACAAATCATTCGCCTGTTATCTCTCCACATCCACCGGACCAGTCGCCGGAACTCTTTACCTGTCCAATATTCACACAGCTTTTTGCAGCGACCGGCCATTGTCATTCACTGCACCTTCCGGTCAGGTGACTTGGAGCTATTACAAGGTTATGGTGCCGCTGGCAAATGTTGCGACTATCAATCCAGTGGTGATGAGGGAGAATCCGACTGAGAAGTACCTCCAGATTGTGACCATTGACGGCCATGATTTCTGGTTCATGGGGTTTGTTAATTATGAAAAAGCATCCAAACATCTTACTCAGAGTATCTCCACTTTCGTAGCGTCTGGATTTGCAGTCCAACCTCCCCCATATGAAAAGCATGCTGGTGCTGAACATGATCATGATCATGATCACAAGAAGGGAGGTGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

380

Amino Acids

41.78

Weight (kDa)

6.03

Isoelectric Point (pI)

57.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 231 - 343 6e-14 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 616
Acc36I ACCTGC 2 cut(s) 211, 654
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 1 cut(s) 320
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 5 cut(s) 318, 320, 323, 423, 884
AcoI YGGCCR 3 cut(s) 576, 821, 967
AfaI GTAC 6 cut(s) 44, 264, 433, 464, 473, 944
AfiI CCNNNNNNNGG 1 cut(s) 773
AgsI TTSAA 2 cut(s) 95, 547
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 551
AluBI AGCT 6 cut(s) 407, 443, 450, 510, 806, 864
AluI AGCT 6 cut(s) 407, 443, 450, 510, 806, 864
Alw26I GTCTC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 5 cut(s) 315, 501, 576, 821, 967
ApeKI GCWGC 5 cut(s) 272, 404, 507, 626, 813
AspS9I GGNCC 1 cut(s) 764
AsuC2I CCSGG 2 cut(s) 368, 389
AsuHPI GGTGA 2 cut(s) 865, 928
AvaII GGWCC 1 cut(s) 764
BaeGI GKGCMC 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 26, 59
BalI TGGCCA 1 cut(s) 578
BanI GGYRCC 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 535
BbvI GCAGC 5 cut(s) 284, 416, 519, 638, 825
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 982
BciT130I CCWGG 1 cut(s) 553
BclI TGATCA 6 cut(s) 214, 220, 484, 1105, 1111, 1117
BcnI CCSGG 2 cut(s) 368, 389
BcoDI GTCTC 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 647
BfuAI ACCTGC 2 cut(s) 211, 654
BglII AGATCT 1 cut(s) 301
BisI GCNGC 9 cut(s) 273, 318, 321, 405, 423, 508, 627, 814, 884
BlsI GCNGC 9 cut(s) 274, 319, 322, 406, 424, 509, 628, 815, 885
Bme1390I CCNGG 3 cut(s) 368, 389, 553
Bme18I GGWCC 1 cut(s) 764
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 764
BmiI GGNNCC 2 cut(s) 557, 882
BmrFI CCNGG 3 cut(s) 368, 389, 553
BmsI GCATC 1 cut(s) 1019
BpiI GAAGAC 1 cut(s) 535
BpmI CTGGAG 4 cut(s) 452, 496, 574, 932
BpuMI CCSGG 2 cut(s) 368, 389
BsaBI GATNNNNATC 5 cut(s) 114, 219, 225, 1110, 1116
BsaJI CCNNGG 3 cut(s) 312, 318, 573
BsaWI WCCGGW 3 cut(s) 620, 761, 845
Bsc4I CCNNNNNNNGG 1 cut(s) 773
Bse118I RCCGGY 1 cut(s) 819
Bse1I ACTGG 4 cut(s) 266, 469, 767, 911
Bse8I GATNNNNATC 5 cut(s) 114, 219, 225, 1110, 1116
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 312, 318, 573
BseGI GGATG 3 cut(s) 100, 755, 1010
BseJI GATNNNNATC 5 cut(s) 114, 219, 225, 1110, 1116
BseLI CCNNNNNNNGG 1 cut(s) 773
BseMII CTCAG 5 cut(s) 246, 327, 458, 927, 1040
BseNI ACTGG 4 cut(s) 266, 469, 767, 911
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 5 cut(s) 284, 416, 519, 638, 825
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 5 cut(s) 317, 503, 578, 823, 969
BshNI GGYRCC 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 820
BsiSI CCGG 7 cut(s) 368, 389, 621, 762, 774, 820, 846
BslFI GGGAC 3 cut(s) 359, 375, 622
BslI CCNNNNNNNGG 1 cut(s) 773
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 3 cut(s) 359, 375, 622
BsnI GGCC 5 cut(s) 317, 503, 578, 823, 969
Bsp1286I GDGCHC 1 cut(s) 350
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 5 cut(s) 318, 320, 323, 423, 884
BspANI GGCC 5 cut(s) 317, 503, 578, 823, 969
BspCNI CTCAG 5 cut(s) 247, 328, 457, 928, 1039
BspEI TCCGGA 1 cut(s) 620
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 3 cut(s) 217, 1108, 1114
BspLI GGNNCC 2 cut(s) 557, 882
BspMAI CTGCAG 1 cut(s) 651
BspMI ACCTGC 2 cut(s) 211, 654
BspT107I GGYRCC 1 cut(s) 880
BsrFI RCCGGY 1 cut(s) 819
BsrI ACTGG 4 cut(s) 266, 469, 767, 911
BssAI RCCGGY 1 cut(s) 819
BssECI CCNNGG 3 cut(s) 312, 318, 573
BssMI GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
BssT1I CCWWGG 2 cut(s) 312, 573
Bst2UI CCWGG 1 cut(s) 553
BstAPI GCANNNNNTGC 1 cut(s) 896
BstC8I GCNNGC 5 cut(s) 501, 505, 651, 888, 1089
BstDEI CTNAG 5 cut(s) 255, 336, 444, 936, 1026
BstDSI CCRYGG 2 cut(s) 318, 573
BstF5I GGATG 3 cut(s) 100, 755, 1010
BstFNI CGCG 1 cut(s) 320
BstKTI GATC 7 cut(s) 217, 223, 304, 487, 1108, 1114, 1120
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
BstMCI CGRYCG 1 cut(s) 820
BstMWI GCNNNNNNNGC 6 cut(s) 77, 317, 326, 513, 632, 896
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 1 cut(s) 1091
BstSCI CCNGG 3 cut(s) 366, 387, 551
BstSFI CTRYAG 1 cut(s) 647
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 5 cut(s) 284, 416, 519, 638, 825
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 5 cut(s) 317, 503, 578, 823, 969
BtgI CCRYGG 2 cut(s) 318, 573
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 3 cut(s) 100, 755, 1010
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 834, 918
BveI ACCTGC 2 cut(s) 211, 654
Cac8I GCNNGC 5 cut(s) 501, 505, 651, 888, 1089
CaiI CAGNNNCTG 1 cut(s) 335
CciI TCATGA 3 cut(s) 217, 1108, 1114
Cfr10I RCCGGY 1 cut(s) 819
Cfr13I GGNCC 1 cut(s) 764
Cfr42I CCGCGG 1 cut(s) 321
CseI GACGC 1 cut(s) 1038
Csp6I GTAC 6 cut(s) 43, 263, 432, 463, 472, 943
CviAII CATG 9 cut(s) 218, 358, 574, 971, 985, 1088, 1103, 1109, 1115
CviQI GTAC 6 cut(s) 43, 263, 432, 463, 472, 943
DdeI CTNAG 5 cut(s) 255, 336, 444, 936, 1026
DpnI GATC 7 cut(s) 216, 222, 303, 486, 1107, 1113, 1119
DpnII GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
DrdI GACNNNNNNGTC 1 cut(s) 616
DseDI GACNNNNNNGTC 1 cut(s) 616
EaeI YGGCCR 3 cut(s) 576, 821, 967
EciI GGCGGA 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 312, 573
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 551
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 312, 573
ErhI CCWWGG 2 cut(s) 312, 573
FaeI CATG 9 cut(s) 221, 361, 577, 974, 988, 1091, 1106, 1112, 1118
FaqI GGGAC 3 cut(s) 359, 375, 622
FatI CATG 9 cut(s) 217, 357, 573, 970, 984, 1087, 1102, 1108, 1114
FauNDI CATATG 1 cut(s) 1078
FbaI TGATCA 6 cut(s) 214, 220, 484, 1105, 1111, 1117
Fnu4HI GCNGC 9 cut(s) 273, 318, 321, 405, 423, 508, 627, 814, 884
FokI GGATG 3 cut(s) 87, 742, 997
Fsp4HI GCNGC 9 cut(s) 273, 318, 321, 405, 423, 508, 627, 814, 884
GluI GCNGC 9 cut(s) 273, 318, 321, 405, 423, 508, 627, 814, 884
GsuI CTGGAG 4 cut(s) 452, 496, 574, 932
HaeIII GGCC 5 cut(s) 317, 503, 578, 823, 969
HapII CCGG 7 cut(s) 368, 389, 621, 762, 774, 820, 846
HgaI GACGC 1 cut(s) 1038
Hin1II CATG 9 cut(s) 221, 361, 577, 974, 988, 1091, 1106, 1112, 1118
HinfI GANTC 4 cut(s) 20, 569, 721, 928
HpaII CCGG 7 cut(s) 368, 389, 621, 762, 774, 820, 846
HphI GGTGA 2 cut(s) 865, 928
Hpy188I TCNGA 4 cut(s) 256, 447, 933, 1029
Hpy188III TCNNGA 9 cut(s) 104, 212, 218, 233, 621, 949, 1053, 1109, 1115
HpyAV CCTTC 2 cut(s) 852, 1120
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 4 cut(s) 649, 813, 839, 1061
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 317, 326, 513, 632, 896
HpyF3I CTNAG 5 cut(s) 255, 336, 444, 936, 1026
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 9 cut(s) 221, 361, 577, 974, 988, 1091, 1106, 1112, 1118
Kpn2I TCCGGA 1 cut(s) 620
Ksp22I TGATCA 6 cut(s) 214, 220, 484, 1105, 1111, 1117
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
LmnI GCTCC 5 cut(s) 211, 515, 555, 623, 861
Lsp1109I GCAGC 5 cut(s) 284, 416, 519, 638, 825
LweI GCATC 1 cut(s) 1019
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 3 cut(s) 853, 955, 1133
MalI GATC 7 cut(s) 216, 222, 303, 486, 1107, 1113, 1119
MboI GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
MboII GAAGA 6 cut(s) 83, 119, 240, 311, 535, 649
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 350
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 2 cut(s) 185, 997
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 2 cut(s) 29, 578
MmeI TCCRAC 2 cut(s) 956, 1090
Mox20I TGGCCA 1 cut(s) 578
MroI TCCGGA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 578
MseI TTAA 3 cut(s) 600, 662, 996
MslI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 2 cut(s) 320, 886
MspI CCGG 7 cut(s) 368, 389, 621, 762, 774, 820, 846
MspR9I CCNGG 3 cut(s) 368, 389, 553
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 6 cut(s) 77, 317, 326, 513, 632, 896
NciI CCSGG 2 cut(s) 368, 389
NcoI CCATGG 1 cut(s) 573
NdeI CATATG 1 cut(s) 1078
NdeII GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
NlaIII CATG 9 cut(s) 221, 361, 577, 974, 988, 1091, 1106, 1112, 1118
NlaIV GGNNCC 2 cut(s) 557, 882
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 3 cut(s) 853, 955, 1133
NspI RCATGY 1 cut(s) 1091
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 3 cut(s) 217, 1108, 1114
PfeI GAWTC 2 cut(s) 721, 928
PfoI TCCNGGA 1 cut(s) 551
PkrI GCNGC 9 cut(s) 274, 319, 322, 406, 424, 509, 628, 815, 885
PleI GAGTC 2 cut(s) 28, 577
PpsI GAGTC 2 cut(s) 28, 577
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 2 cut(s) 557, 882
PspPI GGNCC 1 cut(s) 764
PstI CTGCAG 1 cut(s) 651
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 6 cut(s) 44, 264, 433, 464, 473, 944
RsaNI GTAC 6 cut(s) 43, 263, 432, 463, 472, 943
RseI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
SacII CCGCGG 1 cut(s) 321
SaqAI TTAA 3 cut(s) 600, 662, 996
SatI GCNGC 9 cut(s) 273, 318, 321, 405, 423, 508, 627, 814, 884
Sau3AI GATC 7 cut(s) 214, 220, 301, 484, 1105, 1111, 1117
Sau96I GGNCC 1 cut(s) 764
SbfI CCTGCAGG 1 cut(s) 651
SchI GAGTC 2 cut(s) 29, 578
ScrFI CCNGG 3 cut(s) 368, 389, 553
SdaI CCTGCAGG 1 cut(s) 651
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 1019
SfcI CTRYAG 1 cut(s) 647
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
SphI GCATGC 1 cut(s) 1091
Sse8387I CCTGCAGG 1 cut(s) 651
Sse9I AATT 2 cut(s) 185, 997
SsiI CCGC 5 cut(s) 318, 320, 323, 423, 884
SspI AATATT 1 cut(s) 796
StyD4I CCNGG 3 cut(s) 366, 387, 551
StyI CCWWGG 2 cut(s) 312, 573
TaiI ACGT 1 cut(s) 357
TasI AATT 2 cut(s) 185, 997
TatI WGTACW 1 cut(s) 431
TauI GCSGC 4 cut(s) 320, 323, 425, 886
TfiI GAWTC 2 cut(s) 721, 928
Tru1I TTAA 3 cut(s) 600, 662, 996
Tru9I TTAA 3 cut(s) 600, 662, 996
TscAI CASTG 2 cut(s) 841, 918
TseFI GTSAC 3 cut(s) 853, 955, 1133
TseI GCWGC 5 cut(s) 272, 404, 507, 626, 813
Tsp45I GTSAC 3 cut(s) 853, 955, 1133
TspDTI ATGAA 6 cut(s) 17, 240, 656, 973, 1017, 1095
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 2 cut(s) 841, 918
VpaK11BI GGWCC 1 cut(s) 764
XceI RCATGY 1 cut(s) 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.