Rh4DG293400

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004816
Physical Location & Seq
Forward (+)
51506224 .. 51509527
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG293400.1

Sequence Viewer

Length: 873 bp
ATGTCGAGCAGACCGGAAGACCAGCTTTCTCCCCGGGAGTCTTATCCGCCCACCACAACCGTCCCCGACATTCAGGCCTCGCAACCGCCGCCGCCGCCTCCGCCGTCGGAGGAGGAGGACGCCAAGAAATGGGGGACCCACATAATGGGAACTCCCGCAGCTCCGAACGTCCACCCGGATAACCAGAAAGCTGCTCTATGGAAAGCCGCCGACCACCAGCAAATCCCGCAGCAACCGCTCCAGCAACAACAGCCGTACGTTCAGTACTCTCCGATCAACAAGCCGACCAACAACCCCTTCGAGCCGGTGATCAACCTCTTCAACTCGTGGGGGAACAAGGCCGAGACCATCGCCCGGAACATCTGGCACAACCTCAAAACAGGGCCCTCTGTTTCCGAGGCCGCCTGGGGAAAAGTGAACTTGACGGCCAAGGCGATCACGGAAGGCGGGTTTGAGTCTCTGTTTAAGCAGATTTTCGCTACTGATCCCAACGAGAGGTTGAAGAAGACTTTCGCTTGCTATCTTTCGACTTCCACCGGACCTGTGGCCGGGACTCTGTATCTGTCGACGGCAAGAATCGGTTTTTGCAGCGACCGGCCGTTGACTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATTCCTATGACGAATGTAGGCAGAGTGAACCCTGTGGTGATTACGGAGACCCCGACGCCGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTCGAGAAAGCTTCGCATCACGTGCTAGACAGTGTGTCGAATTTCAGAGCTGTTGGGACCAATGAAGTGCAACAAGTGCAACCTGTTGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

290

Amino Acids

32.15

Weight (kDa)

6.38

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 154 - 263 4.9e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 129, 145
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 566
AclWI GGATC 1 cut(s) 479
AcoI YGGCCR 3 cut(s) 426, 546, 596
AcsI RAATTY 1 cut(s) 817
AcuI CTGAAG 1 cut(s) 710
AcvI CACGTG 1 cut(s) 799
AcyI GRCGYC 2 cut(s) 120, 710
AfaI GTAC 3 cut(s) 257, 266, 722
AfiI CCNNNNNNNGG 6 cut(s) 129, 145, 354, 495, 548, 712
AgsI TTSAA 2 cut(s) 322, 502
AhdI GACNNNNNGTC 1 cut(s) 811
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 6 cut(s) 25, 161, 191, 639, 788, 827
AluI AGCT 6 cut(s) 25, 161, 191, 639, 788, 827
Alw26I GTCTC 3 cut(s) 338, 462, 695
AlwI GGATC 1 cut(s) 479
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 7 cut(s) 75, 339, 383, 399, 426, 546, 596
ApaI GGGCCC 1 cut(s) 387
ApeKI GCWGC 4 cut(s) 158, 191, 229, 588
ApoI RAATTY 1 cut(s) 817
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 5 cut(s) 135, 383, 384, 539, 834
AsuC2I CCSGG 5 cut(s) 34, 35, 176, 355, 550
AsuHPI GGTGA 4 cut(s) 319, 661, 703, 727
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 3 cut(s) 135, 539, 834
BaeGI GKGCMC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 325
BbrPI CACGTG 1 cut(s) 799
BbsI GAAGAC 2 cut(s) 24, 512
BbvI GCAGC 4 cut(s) 170, 178, 241, 600
BccI CCATC 2 cut(s) 356, 737
BceAI ACGGC 5 cut(s) 88, 238, 441, 583, 585
BciT130I CCWGG 1 cut(s) 406
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 5 cut(s) 34, 35, 176, 355, 550
BcoDI GTCTC 3 cut(s) 338, 462, 695
BfaI CTAG 1 cut(s) 803
BisI GCNGC 9 cut(s) 89, 92, 95, 159, 192, 207, 230, 402, 589
BlsI GCNGC 9 cut(s) 90, 93, 96, 160, 193, 208, 231, 403, 590
BmcAI AGTACT 1 cut(s) 266
Bme1390I CCNGG 6 cut(s) 34, 35, 176, 355, 406, 550
Bme18I GGWCC 3 cut(s) 135, 539, 834
BmeRI GACNNNNNGTC 1 cut(s) 811
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 135, 383, 384, 539, 834
BmiI GGNNCC 4 cut(s) 136, 137, 385, 835
BmrFI CCNGG 6 cut(s) 34, 35, 176, 355, 406, 550
BmsI GCATC 1 cut(s) 802
BpiI GAAGAC 2 cut(s) 24, 512
BpmI CTGGAG 1 cut(s) 224
BpuMI CCSGG 5 cut(s) 34, 35, 176, 355, 550
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 120, 710
BsaI GGTCTC 2 cut(s) 338, 695
BsaJI CCNNGG 5 cut(s) 32, 33, 396, 405, 429
BsaWI WCCGGW 2 cut(s) 13, 536
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 145, 354, 495, 548, 712
Bse118I RCCGGY 2 cut(s) 304, 594
BseBI CCWGG 1 cut(s) 406
BseDI CCNNGG 5 cut(s) 32, 33, 396, 405, 429
BseLI CCNNNNNNNGG 6 cut(s) 129, 145, 354, 495, 548, 712
BseRI GAGGAG 2 cut(s) 125, 128
BseSI GKGCMC 1 cut(s) 387
BseX3I CGGCCG 1 cut(s) 596
BseXI GCAGC 4 cut(s) 170, 178, 241, 600
Bsh1285I CGRYCG 2 cut(s) 595, 599
BshFI GGCC 7 cut(s) 77, 341, 385, 401, 428, 548, 598
BsiEI CGRYCG 2 cut(s) 595, 599
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 9 cut(s) 14, 34, 176, 305, 355, 537, 549, 595, 713
BsiWI CGTACG 1 cut(s) 255
BslFI GGGAC 4 cut(s) 47, 148, 565, 847
BslI CCNNNNNNNGG 6 cut(s) 129, 145, 354, 495, 548, 712
BsmAI GTCTC 3 cut(s) 338, 462, 695
BsmFI GGGAC 4 cut(s) 47, 148, 565, 847
BsnI GGCC 7 cut(s) 77, 341, 385, 401, 428, 548, 598
Bso31I GGTCTC 2 cut(s) 338, 695
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 4 cut(s) 273, 309, 435, 484
BspANI GGCC 7 cut(s) 77, 341, 385, 401, 428, 548, 598
BspLI GGNNCC 4 cut(s) 136, 137, 385, 835
BspPI GGATC 1 cut(s) 479
BspTNI GGTCTC 2 cut(s) 338, 695
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 2 cut(s) 304, 594
BssAI RCCGGY 2 cut(s) 304, 594
BssECI CCNNGG 5 cut(s) 32, 33, 396, 405, 429
BssMI GATC 4 cut(s) 273, 309, 435, 484
BssNI GRCGYC 2 cut(s) 120, 710
BssSI CACGAG 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 2 cut(s) 61, 809
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 2 cut(s) 120, 710
BstAPI GCANNNNNTGC 2 cut(s) 799, 853
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 1 cut(s) 517
BstKTI GATC 4 cut(s) 276, 312, 438, 487
BstMAI GTCTC 3 cut(s) 338, 462, 695
BstMBI GATC 4 cut(s) 273, 309, 435, 484
BstMCI CGRYCG 2 cut(s) 595, 599
BstMWI GCNNNNNNNGC 8 cut(s) 88, 94, 100, 226, 235, 250, 799, 853
BstNI CCWGG 1 cut(s) 406
BstSCI CCNGG 6 cut(s) 32, 33, 174, 353, 404, 548
BstSLI GKGCMC 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 170, 178, 241, 600
BstV2I GAAGAC 2 cut(s) 24, 512
BstZI CGGCCG 1 cut(s) 596
BsuRI GGCC 7 cut(s) 77, 341, 385, 401, 428, 548, 598
BtgZI GCGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 814
Cac8I GCNNGC 1 cut(s) 517
Cfr10I RCCGGY 2 cut(s) 304, 594
Cfr13I GGNCC 5 cut(s) 135, 383, 384, 539, 834
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 2 cut(s) 128, 718
Csp6I GTAC 3 cut(s) 256, 265, 721
CviAII CATG 1 cut(s) 749
CviQI GTAC 3 cut(s) 256, 265, 721
DpnI GATC 4 cut(s) 275, 311, 437, 486
DpnII GATC 4 cut(s) 273, 309, 435, 484
DriI GACNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 3 cut(s) 426, 546, 596
EagI CGGCCG 1 cut(s) 596
Eam1104I CTCTTC 1 cut(s) 323
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 323
EciI GGCGGA 2 cut(s) 36, 90
EclXI CGGCCG 1 cut(s) 596
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 2 cut(s) 338, 695
Eco47I GGWCC 3 cut(s) 135, 539, 834
Eco52I CGGCCG 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 710
Eco72I CACGTG 1 cut(s) 799
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 3 cut(s) 135, 383, 384
EcoRII CCWGG 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 1 cut(s) 752
FaiI YATR 5 cut(s) 143, 199, 662, 750, 764
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 4 cut(s) 47, 148, 565, 847
FatI CATG 1 cut(s) 748
FauI CCCGC 3 cut(s) 163, 234, 440
FbaI TGATCA 1 cut(s) 309
FblI GTMKAC 1 cut(s) 566
Fnu4HI GCNGC 9 cut(s) 89, 92, 95, 159, 192, 207, 230, 402, 589
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 9 cut(s) 89, 92, 95, 159, 192, 207, 230, 402, 589
FspBI CTAG 1 cut(s) 803
GluI GCNGC 9 cut(s) 89, 92, 95, 159, 192, 207, 230, 402, 589
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 7 cut(s) 77, 341, 385, 401, 428, 548, 598
HapII CCGG 9 cut(s) 14, 34, 176, 305, 355, 537, 549, 595, 713
HgaI GACGC 2 cut(s) 128, 718
Hin1I GRCGYC 2 cut(s) 120, 710
Hin1II CATG 1 cut(s) 752
HincII GTYRAC 3 cut(s) 567, 603, 775
HindII GTYRAC 3 cut(s) 567, 603, 775
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 5 cut(s) 38, 455, 553, 576, 655
HpaI GTTAAC 1 cut(s) 775
HpaII CCGG 9 cut(s) 14, 34, 176, 305, 355, 537, 549, 595, 713
HphI GGTGA 4 cut(s) 319, 661, 703, 727
Hpy166II GTNNAC 6 cut(s) 172, 418, 567, 603, 682, 775
Hpy188I TCNGA 6 cut(s) 109, 165, 273, 397, 729, 824
Hpy188III TCNNGA 2 cut(s) 621, 781
Hpy8I GTNNAC 6 cut(s) 172, 418, 567, 603, 682, 775
Hpy99I CGWCG 3 cut(s) 109, 571, 712
HpyAV CCTTC 3 cut(s) 307, 437, 734
HpyCH4III ACNGT 2 cut(s) 61, 809
HpyCH4IV ACGT 3 cut(s) 168, 258, 798
HpyCH4V TGCA 3 cut(s) 588, 847, 856
HpyF10VI GCNNNNNNNGC 8 cut(s) 88, 94, 100, 226, 235, 250, 799, 853
HpySE526I ACGT 3 cut(s) 168, 258, 798
Hsp92I GRCGYC 2 cut(s) 120, 710
Hsp92II CATG 1 cut(s) 752
KflI GGGWCCC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 309
KspAI GTTAAC 1 cut(s) 775
Kzo9I GATC 4 cut(s) 273, 309, 435, 484
LmnI GCTCC 4 cut(s) 166, 243, 619, 636
Lsp1109I GCAGC 4 cut(s) 170, 178, 241, 600
LweI GCATC 1 cut(s) 802
MaeI CTAG 1 cut(s) 803
MaeII ACGT 3 cut(s) 168, 258, 798
MaeIII GTNAC 1 cut(s) 733
MalI GATC 4 cut(s) 275, 311, 437, 486
MbiI CCGCTC 1 cut(s) 238
MboI GATC 4 cut(s) 273, 309, 435, 484
MboII GAAGA 4 cut(s) 29, 310, 514, 517
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 817
MlyI GAGTC 3 cut(s) 47, 464, 547
MmeI TCCRAC 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 2 cut(s) 465, 774
MspI CCGG 9 cut(s) 14, 34, 176, 305, 355, 537, 549, 595, 713
MspR9I CCNGG 6 cut(s) 34, 35, 176, 355, 406, 550
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 8 cut(s) 88, 94, 100, 226, 235, 250, 799, 853
NciI CCSGG 5 cut(s) 34, 35, 176, 355, 550
NdeII GATC 4 cut(s) 273, 309, 435, 484
NlaIII CATG 1 cut(s) 752
NlaIV GGNNCC 4 cut(s) 136, 137, 385, 835
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 733
PceI AGGCCT 1 cut(s) 77
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 704
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 2 cut(s) 576, 655
Pfl23II CGTACG 1 cut(s) 255
PflMI CCANNNNNTGG 2 cut(s) 129, 145
PkrI GCNGC 9 cut(s) 90, 93, 96, 160, 193, 208, 231, 403, 590
PleI GAGTC 3 cut(s) 46, 463, 547
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
PpsI GAGTC 3 cut(s) 46, 463, 547
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 404
PspCI CACGTG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 404
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 4 cut(s) 136, 137, 385, 835
PspOMI GGGCCC 1 cut(s) 383
PspPI GGNCC 5 cut(s) 135, 383, 384, 539, 834
PspPPI RGGWCCY 1 cut(s) 135
RsaI GTAC 3 cut(s) 257, 266, 722
RsaNI GTAC 3 cut(s) 256, 265, 721
SalI GTCGAC 1 cut(s) 565
SaqAI TTAA 2 cut(s) 465, 774
SatI GCNGC 9 cut(s) 89, 92, 95, 159, 192, 207, 230, 402, 589
Sau3AI GATC 4 cut(s) 273, 309, 435, 484
Sau96I GGNCC 5 cut(s) 135, 383, 384, 539, 834
ScaI AGTACT 1 cut(s) 266
SchI GAGTC 3 cut(s) 47, 464, 547
ScrFI CCNGG 6 cut(s) 34, 35, 176, 355, 406, 550
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 1 cut(s) 802
SinI GGWCC 3 cut(s) 135, 539, 834
SmaI CCCGGG 1 cut(s) 35
Sse9I AATT 1 cut(s) 817
SseBI AGGCCT 1 cut(s) 77
SspMI CTAG 1 cut(s) 803
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 6 cut(s) 32, 33, 174, 353, 404, 548
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 2 cut(s) 61, 809
TaiI ACGT 3 cut(s) 171, 261, 801
TaqI TCGA 6 cut(s) 5, 300, 527, 566, 780, 815
TasI AATT 1 cut(s) 817
TatI WGTACW 1 cut(s) 264
TauI GCSGC 5 cut(s) 91, 94, 97, 209, 404
TfiI GAWTC 2 cut(s) 576, 655
Tru1I TTAA 2 cut(s) 465, 774
Tru9I TTAA 2 cut(s) 465, 774
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 4 cut(s) 158, 191, 229, 588
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 1 cut(s) 855
TspGWI ACGGA 3 cut(s) 455, 606, 713
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 1 cut(s) 814
Van91I CCANNNNNTGG 2 cut(s) 129, 145
VpaK11BI GGWCC 3 cut(s) 135, 539, 834
XapI RAATTY 1 cut(s) 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 1 cut(s) 803
ZrmI AGTACT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.