MD16G1127100.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004816
Physical Location & Seq
Reverse (-)
9245059 .. 9246171
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1127100.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCAGCTACACCTGAACAAACTCACCCCCATTCCGAACACCCCCCTCAGCAACCACCACCACCAGAGGCGGAGGACACTAAGAAATGGGGGACCCACATCATGGGCACCCCAGCAGCCCCGAACGTCCACCCAGACAACCAGCAAGCCGCCCTATGGAAAGCCGCCGACCACCAGCAAATCCCCCAGCAGCAGCCCTACGTCCAGCACTCTCCGATCGACAAACACACCAACAACCCCTTTGAGCCGGTTATCCAAGCGTTCAATTCCTGGAGCGCTAAGGCGGAGACCATGGCCAGGAACATCTGGTACAACCTCAAAACGGGGCACTCTGTTCCGGAAGCGGCGTGGGGGAAGGTGAACTTGACGGCGAAAGCCTTATCCGAAGGCGGATTCGAGTCGCTGTTTAAGCAGATTTTCGCGACTGACCCGAACGAGAAGTTGAAGAAGACGTTTGCCTGTTATCTTTCGACGTCGACGGGGCCGGTTGCCGGGACTCAGTATCTGTCGACTGCTAGACTGGCTTTTTGCAGCGATCGTCCTCTGACTTTTACTTCTCCGTCTGGACAAGCAGCTTGGAGTTACTACAAGGTGTCTATACCTTTGGGAAATATAAGCACCGTGAACCCTGTGGTGACGAAAGAGAATCCGCCGGAGAAGTACATTCAGATCGCCACCACAGATGGCCATGAATTCTGGTTTATGGGTTTTGTTAATTTCGACAAGGCATCCCATCACATCTTCGAATCTGTAGCAGATTTCAGAACTGCTGGGAATGCGGTGCAGCAAGTGCAGCCGGTTCCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

270

Amino Acids

29.7

Weight (kDa)

6.45

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 135 - 247 7.1e-16 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 476
AccB1I GGYRCC 1 cut(s) 107
AccB7I CCANNNNNTGG 1 cut(s) 103
AccI GTMKAC 2 cut(s) 476, 509
AccII CGCG 1 cut(s) 422
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 8 cut(s) 71, 150, 165, 284, 344, 390, 650, 779
AcoI YGGCCR 2 cut(s) 294, 685
AcsI RAATTY 1 cut(s) 692
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 2 cut(s) 311, 662
AfeI AGCGCT 1 cut(s) 277
AfiI CCNNNNNNNGG 6 cut(s) 103, 156, 297, 322, 491, 803
AgsI TTSAA 2 cut(s) 265, 445
AjnI CCWGG 3 cut(s) 269, 296, 802
AluBI AGCT 2 cut(s) 8, 575
AluI AGCT 2 cut(s) 8, 575
Alw26I GTCTC 1 cut(s) 281
AlwNI CAGNNNCTG 1 cut(s) 505
Aor13HI TCCGGA 1 cut(s) 337
Aor51HI AGCGCT 1 cut(s) 277
AoxI GGCC 3 cut(s) 294, 482, 685
ApeKI GCWGC 8 cut(s) 5, 116, 190, 193, 531, 572, 784, 793
ApoI RAATTY 1 cut(s) 692
ArsI GACNNNNNNTTYG 2 cut(s) 510, 542
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 93, 482
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 3 cut(s) 17, 370, 646
AsuII TTCGAA 1 cut(s) 744
AvaII GGWCC 1 cut(s) 93
BaeGI GKGCMC 2 cut(s) 110, 330
BalI TGGCCA 2 cut(s) 296, 687
BanI GGYRCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 455
BbvCI CCTCAGC 1 cut(s) 48
BbvI GCAGC 8 cut(s) 17, 128, 202, 205, 543, 584, 796, 805
BccI CCATC 2 cut(s) 677, 741
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 3 cut(s) 271, 298, 804
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 2 cut(s) 516, 808
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 279
Bme1390I CCNGG 4 cut(s) 271, 298, 493, 804
Bme18I GGWCC 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 93, 482
BmiI GGNNCC 5 cut(s) 94, 95, 109, 483, 801
BmrFI CCNGG 4 cut(s) 271, 298, 493, 804
BmsI GCATC 1 cut(s) 737
BpiI GAAGAC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 292
Bpu10I CCTNAGC 2 cut(s) 48, 279
Bpu14I TTCGAA 1 cut(s) 744
BpuMI CCSGG 1 cut(s) 493
BsaHI GRCGYC 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 6 cut(s) 103, 156, 297, 322, 491, 803
Bse118I RCCGGY 3 cut(s) 247, 484, 796
Bse1I ACTGG 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 337
BseBI CCWGG 3 cut(s) 271, 298, 804
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 728
BseLI CCNNNNNNNGG 6 cut(s) 103, 156, 297, 322, 491, 803
BseMII CTCAG 2 cut(s) 62, 512
BseNI ACTGG 1 cut(s) 525
BseSI GKGCMC 2 cut(s) 110, 330
BseXI GCAGC 8 cut(s) 17, 128, 202, 205, 543, 584, 796, 805
BseYI CCCAGC 3 cut(s) 112, 186, 770
BsgI GTGCAG 1 cut(s) 803
Bsh1236I CGCG 1 cut(s) 422
Bsh1285I CGRYCG 2 cut(s) 219, 538
BshFI GGCC 3 cut(s) 296, 484, 687
BshNI GGYRCC 1 cut(s) 107
BsiEI CGRYCG 2 cut(s) 219, 538
BsiSI CCGG 6 cut(s) 248, 338, 485, 492, 653, 797
BslFI GGGAC 2 cut(s) 106, 508
BslI CCNNNNNNNGG 6 cut(s) 103, 156, 297, 322, 491, 803
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 2 cut(s) 106, 508
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 3 cut(s) 296, 484, 687
Bso31I GGTCTC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 744
Bsp1286I GDGCHC 2 cut(s) 110, 330
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 3 cut(s) 216, 535, 669
Bsp19I CCATGG 1 cut(s) 291
Bsp68I TCGCGA 1 cut(s) 422
BspACI CCGC 8 cut(s) 71, 150, 165, 284, 344, 390, 650, 779
BspANI GGCC 3 cut(s) 296, 484, 687
BspCNI CTCAG 2 cut(s) 61, 511
BspEI TCCGGA 1 cut(s) 337
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 5 cut(s) 94, 95, 109, 483, 801
BspT104I TTCGAA 1 cut(s) 744
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 281
BsrFI RCCGGY 3 cut(s) 247, 484, 796
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 3 cut(s) 247, 484, 796
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 3 cut(s) 216, 535, 669
BssNI GRCGYC 1 cut(s) 473
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 3 cut(s) 271, 298, 804
Bst4CI ACNGT 1 cut(s) 622
BstACI GRCGYC 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 790
BstBI TTCGAA 1 cut(s) 744
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 48, 81, 279, 498
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 728
BstFNI CGCG 1 cut(s) 422
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 3 cut(s) 219, 538, 672
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 216, 535, 669
BstMCI CGRYCG 2 cut(s) 219, 538
BstMWI GCNNNNNNNGC 5 cut(s) 409, 521, 776, 790, 793
BstNI CCWGG 3 cut(s) 271, 298, 804
BstSCI CCNGG 4 cut(s) 269, 296, 491, 802
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 2 cut(s) 110, 330
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 8 cut(s) 17, 128, 202, 205, 543, 584, 796, 805
BstV2I GAAGAC 1 cut(s) 455
BsuRI GGCC 3 cut(s) 296, 484, 687
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 728
BtuMI TCGCGA 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 147
CaiI CAGNNNCTG 1 cut(s) 505
CfoI GCGC 1 cut(s) 278
Cfr10I RCCGGY 3 cut(s) 247, 484, 796
Cfr13I GGNCC 2 cut(s) 93, 482
Csp6I GTAC 2 cut(s) 310, 661
CviAII CATG 3 cut(s) 103, 292, 689
CviQI GTAC 2 cut(s) 310, 661
DdeI CTNAG 4 cut(s) 48, 81, 279, 498
DpnI GATC 3 cut(s) 218, 537, 671
DpnII GATC 3 cut(s) 216, 535, 669
EaeI YGGCCR 2 cut(s) 294, 685
EciI GGCGGA 4 cut(s) 86, 299, 405, 639
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 93
Eco47III AGCGCT 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 3 cut(s) 269, 296, 802
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 3 cut(s) 106, 295, 692
FaiI YATR 7 cut(s) 104, 157, 293, 599, 614, 690, 704
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 2 cut(s) 106, 508
FatI CATG 3 cut(s) 102, 291, 688
FblI GTMKAC 2 cut(s) 476, 509
FokI GGATG 1 cut(s) 715
FspBI CTAG 2 cut(s) 516, 808
GlaI GCGC 1 cut(s) 277
GsaI CCCAGC 3 cut(s) 116, 190, 774
GsuI CTGGAG 1 cut(s) 292
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 3 cut(s) 296, 484, 687
HapII CCGG 6 cut(s) 248, 338, 485, 492, 653, 797
HhaI GCGC 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 3 cut(s) 106, 295, 692
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 2 cut(s) 477, 510
HindII GTYRAC 2 cut(s) 477, 510
HinfI GANTC 5 cut(s) 393, 398, 496, 646, 746
HpaII CCGG 6 cut(s) 248, 338, 485, 492, 653, 797
HphI GGTGA 3 cut(s) 17, 370, 646
Hpy166II GTNNAC 5 cut(s) 130, 361, 477, 510, 625
Hpy188I TCNGA 6 cut(s) 37, 216, 385, 546, 669, 764
Hpy188III TCNNGA 3 cut(s) 338, 421, 564
Hpy8I GTNNAC 5 cut(s) 130, 361, 477, 510, 625
Hpy99I CGWCG 3 cut(s) 475, 478, 481
HpyAV CCTTC 2 cut(s) 349, 380
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 4 cut(s) 126, 201, 452, 473
HpyCH4V TGCA 3 cut(s) 531, 784, 793
HpyF10VI GCNNNNNNNGC 5 cut(s) 409, 521, 776, 790, 793
HpyF3I CTNAG 4 cut(s) 48, 81, 279, 498
HpySE526I ACGT 4 cut(s) 126, 201, 452, 473
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 3 cut(s) 106, 295, 692
HspAI GCGC 1 cut(s) 276
KflI GGGWCCC 1 cut(s) 93
Kpn2I TCCGGA 1 cut(s) 337
Kzo9I GATC 3 cut(s) 216, 535, 669
LmnI GCTCC 1 cut(s) 273
Lsp1109I GCAGC 8 cut(s) 17, 128, 202, 205, 543, 584, 796, 805
LweI GCATC 1 cut(s) 737
MaeI CTAG 2 cut(s) 516, 808
MaeII ACGT 4 cut(s) 126, 201, 452, 473
MaeIII GTNAC 2 cut(s) 581, 634
MalI GATC 3 cut(s) 218, 537, 671
MboI GATC 3 cut(s) 216, 535, 669
MboII GAAGA 3 cut(s) 457, 460, 733
MhlI GDGCHC 2 cut(s) 110, 330
MlsI TGGCCA 2 cut(s) 296, 687
MluCI AATT 3 cut(s) 265, 692, 715
MluNI TGGCCA 2 cut(s) 296, 687
MlyI GAGTC 2 cut(s) 407, 490
MnlI CCTC 5 cut(s) 57, 61, 67, 326, 552
Mox20I TGGCCA 2 cut(s) 296, 687
MroI TCCGGA 1 cut(s) 337
MscI TGGCCA 2 cut(s) 296, 687
MseI TTAA 2 cut(s) 408, 714
Msp20I TGGCCA 2 cut(s) 296, 687
MspI CCGG 6 cut(s) 248, 338, 485, 492, 653, 797
MspR9I CCNGG 4 cut(s) 271, 298, 493, 804
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 271, 298, 804
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 5 cut(s) 409, 521, 776, 790, 793
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 1 cut(s) 291
NdeII GATC 3 cut(s) 216, 535, 669
NlaIII CATG 3 cut(s) 106, 295, 692
NlaIV GGNNCC 5 cut(s) 94, 95, 109, 483, 801
NmuCI GTSAC 1 cut(s) 634
NruI TCGCGA 1 cut(s) 422
NspV TTCGAA 1 cut(s) 744
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 3 cut(s) 393, 646, 746
PflMI CCANNNNNTGG 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 269
Ple19I CGATCG 2 cut(s) 219, 538
PleI GAGTC 2 cut(s) 406, 490
PpsI GAGTC 2 cut(s) 406, 490
PpuMI RGGWCCY 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 3 cut(s) 269, 296, 802
PspFI CCCAGC 3 cut(s) 112, 186, 770
PspGI CCWGG 3 cut(s) 269, 296, 802
PspN4I GGNNCC 5 cut(s) 94, 95, 109, 483, 801
PspPI GGNCC 2 cut(s) 93, 482
PspPPI RGGWCCY 1 cut(s) 93
PsrI GAACNNNNNNTAC 2 cut(s) 293, 325
PstNI CAGNNNCTG 1 cut(s) 505
PvuI CGATCG 2 cut(s) 219, 538
RruI TCGCGA 1 cut(s) 422
RsaI GTAC 2 cut(s) 311, 662
RsaNI GTAC 2 cut(s) 310, 661
SalI GTCGAC 2 cut(s) 475, 508
SaqAI TTAA 2 cut(s) 408, 714
Sau3AI GATC 3 cut(s) 216, 535, 669
Sau96I GGNCC 2 cut(s) 93, 482
SchI GAGTC 2 cut(s) 407, 490
ScrFI CCNGG 4 cut(s) 271, 298, 493, 804
SduI GDGCHC 2 cut(s) 110, 330
SfaNI GCATC 1 cut(s) 737
SfcI CTRYAG 1 cut(s) 750
SfuI TTCGAA 1 cut(s) 744
SgrDI CGTCGACG 1 cut(s) 475
SinI GGWCC 1 cut(s) 93
Sse9I AATT 3 cut(s) 265, 692, 715
SsiI CCGC 8 cut(s) 71, 150, 165, 284, 344, 390, 650, 779
SspMI CTAG 2 cut(s) 516, 808
StyD4I CCNGG 4 cut(s) 269, 296, 491, 802
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 4 cut(s) 129, 204, 455, 476
TaqI TCGA 7 cut(s) 219, 396, 470, 476, 509, 720, 744
TasI AATT 3 cut(s) 265, 692, 715
TatI WGTACW 1 cut(s) 660
TauI GCSGC 3 cut(s) 152, 167, 347
TfiI GAWTC 3 cut(s) 393, 646, 746
Tru1I TTAA 2 cut(s) 408, 714
Tru9I TTAA 2 cut(s) 408, 714
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 8 cut(s) 5, 116, 190, 193, 531, 572, 784, 793
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 1 cut(s) 705
TspGWI ACGGA 1 cut(s) 549
Van91I CCANNNNNTGG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 93
XapI RAATTY 1 cut(s) 692
XmiI GTMKAC 2 cut(s) 476, 509
XspI CTAG 2 cut(s) 516, 808
ZraI GACGTC 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.