Rw4G025180

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004816
Physical Location & Seq
Forward (+)
51182501 .. 51183450
950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025180.1

Sequence Viewer

Length: 864 bp
ATGTCGAGCAGACCGGAAGACCAGCTTTCTCCCCGGGAGTCTTATCCGCCCACCACAACCGTCCCCGACATTCAGGCCTCGCAACCGCCTCCGCCGTCGGAGGAGGAGGACGCCAAGAAATGGGGGACCCACATAATGGGAACTCCCGCAGCTCCGAACGTCCACCCGGATAACCAGAAAGCTGCTCTATGGAAAGCCGCCGACCACCAGCAAATCCCGCAGCAACCGCTCCAGCAACAACAGCCGTACGTTCAGTACTCTCCGATCAACAAGCCGACCAACAACCCCTTCGAGCCGGTGATCAACCTCTTCAACTCGTGGGGGAACAAGGCCGAGACCATCGCCCGGAACATCTGGCACAACCTCAAAACAGGGCCCTCTGTTTCCGAGGCCGCCTGGGGAAAAGTGAACTTGACGGCCAAGGCGATCACGGAAGGCGGGTTTGAGTCTCTGTTTAAGCAGATTTTCGCTACTGATCCCAACGAGAGGTTGAAGAAGACTTTCGCTTGCTATCTTTCGACTTCCACCGGACCTGTGGCCGGGACTCTGTATCTGTCGACGGCAAGAATCGGTTTTTGCAGCGACCGGCCGTTGACTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATTCCTATGACGAATGTAGGCAGAGTGAACCCTGTGGTGATTACGGAGACCCCGACGCCGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTCGAGAAAGCTTCGCATCACGTGCTAGACAGTGTGTCGAATTTCAGAGCTGTTGGGACCAATGAAGTGCAACAAGTGCAACCTGTTGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

287

Amino Acids

31.85

Weight (kDa)

6.38

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 151 - 260 4.7e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 120, 136
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 557
AciI CCGC 9 cut(s) 47, 86, 92, 147, 198, 218, 227, 393, 438
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 3 cut(s) 417, 537, 587
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 1 cut(s) 701
AcvI CACGTG 1 cut(s) 790
AcyI GRCGYC 2 cut(s) 111, 701
AfaI GTAC 3 cut(s) 248, 257, 713
AfiI CCNNNNNNNGG 6 cut(s) 120, 136, 345, 486, 539, 703
AgsI TTSAA 2 cut(s) 313, 493
AhdI GACNNNNNGTC 1 cut(s) 802
AjnI CCWGG 1 cut(s) 395
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 6 cut(s) 25, 152, 182, 630, 779, 818
AluI AGCT 6 cut(s) 25, 152, 182, 630, 779, 818
Alw26I GTCTC 3 cut(s) 329, 453, 686
AlwI GGATC 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 7 cut(s) 75, 330, 374, 390, 417, 537, 587
ApaI GGGCCC 1 cut(s) 378
ApeKI GCWGC 4 cut(s) 149, 182, 220, 579
ApoI RAATTY 1 cut(s) 808
Asp700I GAANNNNTTC 1 cut(s) 500
AspS9I GGNCC 5 cut(s) 126, 374, 375, 530, 825
AsuC2I CCSGG 5 cut(s) 34, 35, 167, 346, 541
AsuHPI GGTGA 4 cut(s) 310, 652, 694, 718
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 3 cut(s) 126, 530, 825
BaeGI GKGCMC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 378
BauI CACGAG 1 cut(s) 316
BbrPI CACGTG 1 cut(s) 790
BbsI GAAGAC 2 cut(s) 24, 503
BbvI GCAGC 4 cut(s) 161, 169, 232, 591
BccI CCATC 2 cut(s) 347, 728
BceAI ACGGC 5 cut(s) 79, 229, 432, 574, 576
BciT130I CCWGG 1 cut(s) 397
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 5 cut(s) 34, 35, 167, 346, 541
BcoDI GTCTC 3 cut(s) 329, 453, 686
BfaI CTAG 1 cut(s) 794
BisI GCNGC 6 cut(s) 150, 183, 198, 221, 393, 580
BlsI GCNGC 6 cut(s) 151, 184, 199, 222, 394, 581
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 6 cut(s) 34, 35, 167, 346, 397, 541
Bme18I GGWCC 3 cut(s) 126, 530, 825
BmeRI GACNNNNNGTC 1 cut(s) 802
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 126, 374, 375, 530, 825
BmiI GGNNCC 4 cut(s) 127, 128, 376, 826
BmrFI CCNGG 6 cut(s) 34, 35, 167, 346, 397, 541
BmsI GCATC 1 cut(s) 793
BpiI GAAGAC 2 cut(s) 24, 503
BpmI CTGGAG 1 cut(s) 215
BpuMI CCSGG 5 cut(s) 34, 35, 167, 346, 541
BsaAI YACGTR 1 cut(s) 790
BsaHI GRCGYC 2 cut(s) 111, 701
BsaI GGTCTC 2 cut(s) 329, 686
BsaJI CCNNGG 5 cut(s) 32, 33, 387, 396, 420
BsaWI WCCGGW 2 cut(s) 13, 527
Bsc4I CCNNNNNNNGG 6 cut(s) 120, 136, 345, 486, 539, 703
Bse118I RCCGGY 2 cut(s) 295, 585
BseBI CCWGG 1 cut(s) 397
BseDI CCNNGG 5 cut(s) 32, 33, 387, 396, 420
BseLI CCNNNNNNNGG 6 cut(s) 120, 136, 345, 486, 539, 703
BseRI GAGGAG 2 cut(s) 116, 119
BseSI GKGCMC 1 cut(s) 378
BseX3I CGGCCG 1 cut(s) 587
BseXI GCAGC 4 cut(s) 161, 169, 232, 591
Bsh1285I CGRYCG 2 cut(s) 586, 590
BshFI GGCC 7 cut(s) 77, 332, 376, 392, 419, 539, 589
BsiEI CGRYCG 2 cut(s) 586, 590
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 9 cut(s) 14, 34, 167, 296, 346, 528, 540, 586, 704
BsiWI CGTACG 1 cut(s) 246
BslFI GGGAC 4 cut(s) 47, 139, 556, 838
BslI CCNNNNNNNGG 6 cut(s) 120, 136, 345, 486, 539, 703
BsmAI GTCTC 3 cut(s) 329, 453, 686
BsmFI GGGAC 4 cut(s) 47, 139, 556, 838
BsnI GGCC 7 cut(s) 77, 332, 376, 392, 419, 539, 589
Bso31I GGTCTC 2 cut(s) 329, 686
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 4 cut(s) 264, 300, 426, 475
BspACI CCGC 9 cut(s) 47, 86, 92, 147, 198, 218, 227, 393, 438
BspANI GGCC 7 cut(s) 77, 332, 376, 392, 419, 539, 589
BspLI GGNNCC 4 cut(s) 127, 128, 376, 826
BspPI GGATC 1 cut(s) 470
BspTNI GGTCTC 2 cut(s) 329, 686
BsrBI CCGCTC 1 cut(s) 229
BsrFI RCCGGY 2 cut(s) 295, 585
BssAI RCCGGY 2 cut(s) 295, 585
BssECI CCNNGG 5 cut(s) 32, 33, 387, 396, 420
BssMI GATC 4 cut(s) 264, 300, 426, 475
BssNI GRCGYC 2 cut(s) 111, 701
BssSI CACGAG 1 cut(s) 316
BssT1I CCWWGG 1 cut(s) 420
Bst2BI CACGAG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 2 cut(s) 61, 800
Bst6I CTCTTC 1 cut(s) 314
BstACI GRCGYC 2 cut(s) 111, 701
BstAPI GCANNNNNTGC 2 cut(s) 790, 844
BstBAI YACGTR 1 cut(s) 790
BstC8I GCNNGC 1 cut(s) 508
BstKTI GATC 4 cut(s) 267, 303, 429, 478
BstMAI GTCTC 3 cut(s) 329, 453, 686
BstMBI GATC 4 cut(s) 264, 300, 426, 475
BstMCI CGRYCG 2 cut(s) 586, 590
BstMWI GCNNNNNNNGC 5 cut(s) 217, 226, 241, 790, 844
BstNI CCWGG 1 cut(s) 397
BstSCI CCNGG 6 cut(s) 32, 33, 165, 344, 395, 539
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 161, 169, 232, 591
BstV2I GAAGAC 2 cut(s) 24, 503
BstZI CGGCCG 1 cut(s) 587
BsuRI GGCC 7 cut(s) 77, 332, 376, 392, 419, 539, 589
BtgZI GCGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 805
Cac8I GCNNGC 1 cut(s) 508
Cfr10I RCCGGY 2 cut(s) 295, 585
Cfr13I GGNCC 5 cut(s) 126, 374, 375, 530, 825
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 2 cut(s) 119, 709
Csp6I GTAC 3 cut(s) 247, 256, 712
CviAII CATG 1 cut(s) 740
CviQI GTAC 3 cut(s) 247, 256, 712
DpnI GATC 4 cut(s) 266, 302, 428, 477
DpnII GATC 4 cut(s) 264, 300, 426, 475
DriI GACNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 3 cut(s) 417, 537, 587
EagI CGGCCG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 314
Eam1105I GACNNNNNGTC 1 cut(s) 802
EarI CTCTTC 1 cut(s) 314
EciI GGCGGA 2 cut(s) 36, 81
EclXI CGGCCG 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 2 cut(s) 329, 686
Eco47I GGWCC 3 cut(s) 126, 530, 825
Eco52I CGGCCG 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 701
Eco72I CACGTG 1 cut(s) 790
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 3 cut(s) 126, 374, 375
EcoRII CCWGG 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 1 cut(s) 743
FaiI YATR 5 cut(s) 134, 190, 653, 741, 755
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 4 cut(s) 47, 139, 556, 838
FatI CATG 1 cut(s) 739
FauI CCCGC 3 cut(s) 154, 225, 431
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 6 cut(s) 150, 183, 198, 221, 393, 580
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 6 cut(s) 150, 183, 198, 221, 393, 580
FspBI CTAG 1 cut(s) 794
GluI GCNGC 6 cut(s) 150, 183, 198, 221, 393, 580
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 7 cut(s) 77, 332, 376, 392, 419, 539, 589
HapII CCGG 9 cut(s) 14, 34, 167, 296, 346, 528, 540, 586, 704
HgaI GACGC 2 cut(s) 119, 709
Hin1I GRCGYC 2 cut(s) 111, 701
Hin1II CATG 1 cut(s) 743
HincII GTYRAC 3 cut(s) 558, 594, 766
HindII GTYRAC 3 cut(s) 558, 594, 766
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 5 cut(s) 38, 446, 544, 567, 646
HpaI GTTAAC 1 cut(s) 766
HpaII CCGG 9 cut(s) 14, 34, 167, 296, 346, 528, 540, 586, 704
HphI GGTGA 4 cut(s) 310, 652, 694, 718
Hpy166II GTNNAC 6 cut(s) 163, 409, 558, 594, 673, 766
Hpy188I TCNGA 6 cut(s) 100, 156, 264, 388, 720, 815
Hpy188III TCNNGA 2 cut(s) 612, 772
Hpy8I GTNNAC 6 cut(s) 163, 409, 558, 594, 673, 766
Hpy99I CGWCG 3 cut(s) 100, 562, 703
HpyAV CCTTC 3 cut(s) 298, 428, 725
HpyCH4III ACNGT 2 cut(s) 61, 800
HpyCH4IV ACGT 3 cut(s) 159, 249, 789
HpyCH4V TGCA 3 cut(s) 579, 838, 847
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 226, 241, 790, 844
HpySE526I ACGT 3 cut(s) 159, 249, 789
Hsp92I GRCGYC 2 cut(s) 111, 701
Hsp92II CATG 1 cut(s) 743
KflI GGGWCCC 1 cut(s) 126
Ksp22I TGATCA 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 4 cut(s) 264, 300, 426, 475
LmnI GCTCC 4 cut(s) 157, 234, 610, 627
Lsp1109I GCAGC 4 cut(s) 161, 169, 232, 591
LweI GCATC 1 cut(s) 793
MaeI CTAG 1 cut(s) 794
MaeII ACGT 3 cut(s) 159, 249, 789
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 266, 302, 428, 477
MbiI CCGCTC 1 cut(s) 229
MboI GATC 4 cut(s) 264, 300, 426, 475
MboII GAAGA 4 cut(s) 29, 301, 505, 508
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 1 cut(s) 808
MlyI GAGTC 3 cut(s) 47, 455, 538
MmeI TCCRAC 1 cut(s) 78
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 2 cut(s) 456, 765
MspI CCGG 9 cut(s) 14, 34, 167, 296, 346, 528, 540, 586, 704
MspR9I CCNGG 6 cut(s) 34, 35, 167, 346, 397, 541
MvaI CCWGG 1 cut(s) 397
MwoI GCNNNNNNNGC 5 cut(s) 217, 226, 241, 790, 844
NciI CCSGG 5 cut(s) 34, 35, 167, 346, 541
NdeII GATC 4 cut(s) 264, 300, 426, 475
NlaIII CATG 1 cut(s) 743
NlaIV GGNNCC 4 cut(s) 127, 128, 376, 826
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 724
PceI AGGCCT 1 cut(s) 77
PcsI WCGNNNNNNNCGW 2 cut(s) 422, 695
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 2 cut(s) 567, 646
Pfl23II CGTACG 1 cut(s) 246
PflMI CCANNNNNTGG 2 cut(s) 120, 136
PkrI GCNGC 6 cut(s) 151, 184, 199, 222, 394, 581
PleI GAGTC 3 cut(s) 46, 454, 538
PmaCI CACGTG 1 cut(s) 790
PmlI CACGTG 1 cut(s) 790
PpsI GAGTC 3 cut(s) 46, 454, 538
Ppu21I YACGTR 1 cut(s) 790
PpuMI RGGWCCY 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 395
PspCI CACGTG 1 cut(s) 790
PspGI CCWGG 1 cut(s) 395
PspLI CGTACG 1 cut(s) 246
PspN4I GGNNCC 4 cut(s) 127, 128, 376, 826
PspOMI GGGCCC 1 cut(s) 374
PspPI GGNCC 5 cut(s) 126, 374, 375, 530, 825
PspPPI RGGWCCY 1 cut(s) 126
RsaI GTAC 3 cut(s) 248, 257, 713
RsaNI GTAC 3 cut(s) 247, 256, 712
SalI GTCGAC 1 cut(s) 556
SaqAI TTAA 2 cut(s) 456, 765
SatI GCNGC 6 cut(s) 150, 183, 198, 221, 393, 580
Sau3AI GATC 4 cut(s) 264, 300, 426, 475
Sau96I GGNCC 5 cut(s) 126, 374, 375, 530, 825
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 3 cut(s) 47, 455, 538
ScrFI CCNGG 6 cut(s) 34, 35, 167, 346, 397, 541
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 1 cut(s) 793
SinI GGWCC 3 cut(s) 126, 530, 825
SmaI CCCGGG 1 cut(s) 35
Sse9I AATT 1 cut(s) 808
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 9 cut(s) 47, 86, 92, 147, 198, 218, 227, 393, 438
SspMI CTAG 1 cut(s) 794
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 6 cut(s) 32, 33, 165, 344, 395, 539
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 61, 800
TaiI ACGT 3 cut(s) 162, 252, 792
TaqI TCGA 6 cut(s) 5, 291, 518, 557, 771, 806
TasI AATT 1 cut(s) 808
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 200, 395
TfiI GAWTC 2 cut(s) 567, 646
Tru1I TTAA 2 cut(s) 456, 765
Tru9I TTAA 2 cut(s) 456, 765
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 4 cut(s) 149, 182, 220, 579
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 1 cut(s) 846
TspGWI ACGGA 3 cut(s) 446, 597, 704
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 1 cut(s) 805
Van91I CCANNNNNTGG 2 cut(s) 120, 136
VpaK11BI GGWCC 3 cut(s) 126, 530, 825
XapI RAATTY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 1 cut(s) 794
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.