Rroxscaffold_2G00076600

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004816
Physical Location & Seq
Forward (+)
372049 .. 373748
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076600.1

Sequence Viewer

Length: 1431 bp
ATGAACAACAGCAACCAAGAATCTCAACCACCTATGTACCCCTCCATTCCTCCTCCCCTCACGGCACACGAAGCAAACCCTTTTCTTCAATCTCATCCTACTCCCGCCCCAGAAGATATCAATCCATTTTCTCAAACCACTTCCCCACCAGAAGCCCCAAAACCTCAAACCCCCTCCATTACTGCCGACCACTTAGAAAAATGGCAACCAGCTCCTCATGATCATCATCAACAACAAGATATCCCATCATCTTCATCTGAGCAAGTACCAGTAGCAGCACCACCACCCACCTTTGACAGCGAAGATCTCAAAGCCAAGGCCGCGGCGGAGGCAGAGCCTGAGAAATGGGGCACTCACGTCATGGGACACCCGGCTGTCCCAACTTGCCACCCGGACAACAAAAAGGCAGCTCTATGGGGAGTTGCCGCCGATGGTACAGAAAAAGCTCAGAGCTTCCACTATCCGTACCTGCAGTACCAACCCATTGATCACAACAACAAGGCCAGCAGCTCCAGCAATCCCACTCAATCTATGCTCAATGTCTTCAACTCCTGGAGCCACAAAGCCGAGTCCATGGCCAACAACATCTGGCACAACCTTAAAACAGGGACATCTGTGTCCGGAGCAGCGTGGGCGAAGATGAACCTGCAGGCGAAAGCATTAACAGGAGGTGGATTTGAGGCTATCTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTCCACAAATCATTCGCCTGTTATCTCTCCACATCCACCGGACCAGTCGCCGGAACTCTTTACCTGTCCAATATTCACACAGCTTTTTGCAGCGACCGGCCATTGTCATTCACTGCACCTTCCGGTCAGGTGACTTGGAGCTATTACAAGGTTATGGTGCCGCTGGCAAATGTTGCGACTATCAATCCAGTGGTGATGAGGGAGAATCCGACTGAGAAGTACCTCCAGATTGTGACCATTGACGGCCATGATTTCTGGTTCATGGGGTTTGTTAATTATGAAAAAGCATCCAAACATCTTACTCAGAGTATCTCCACTTTCGTAGCGTCTGGATTTGTAGTCCAACCTCCCCCATATGAAAAGCATGCTGGTGCTGAACATGATCATGATCATGATCACAAGAAGGGGGTGACAACTAGTACTAGTCTAGTTCATTGCCATTTGCCTGTGTTTTATCTACTGCTGAATTTGAGTTCTCTGCGTTCTCGTTTACATACAATAAGAGCATATATGGATTCATTGATTGTATTATTACTATTGCTTGTTTCATTTTGTGCTACATTTATACTGCTCTGCTTTCAATTTCTTTCCATCAAGAAATACATGTTGCAACTTCTCTATGCTGGTGTTGTTGTTCCGAATCGACCATTCCAACTATGTGATAAGAGGACTGGTGTTCGGAAAGAATTCAAAAGAGGAAATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

476

Amino Acids

52.88

Weight (kDa)

7.38

Isoelectric Point (pI)

56.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 231 - 343 9.7e-14 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 616
Acc36I ACCTGC 2 cut(s) 477, 654
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 6 cut(s) 105, 321, 323, 326, 426, 884
AcoI YGGCCR 3 cut(s) 576, 821, 967
AcsI RAATTY 2 cut(s) 1189, 1409
AfaI GTAC 7 cut(s) 38, 267, 436, 467, 476, 944, 1144
AfiI CCNNNNNNNGG 1 cut(s) 773
AflIII ACRYGT 1 cut(s) 1326
AgsI TTSAA 4 cut(s) 89, 547, 1304, 1414
AhlI ACTAGT 2 cut(s) 1139, 1145
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 1 cut(s) 551
AluBI AGCT 8 cut(s) 212, 410, 446, 453, 510, 724, 806, 864
AluI AGCT 8 cut(s) 212, 410, 446, 453, 510, 724, 806, 864
AlwNI CAGNNNCTG 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 5 cut(s) 318, 501, 576, 821, 967
ApeKI GCWGC 5 cut(s) 275, 407, 507, 626, 813
ApoI RAATTY 2 cut(s) 1189, 1409
Asp700I GAANNNNTTC 1 cut(s) 1409
AspS9I GGNCC 1 cut(s) 764
AsuC2I CCSGG 2 cut(s) 371, 392
AsuHPI GGTGA 3 cut(s) 865, 928, 1144
AvaII GGWCC 1 cut(s) 764
BaeGI GKGCMC 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BalI TGGCCA 1 cut(s) 578
BanI GGYRCC 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 535
BbvI GCAGC 5 cut(s) 287, 419, 519, 638, 825
BccI CCATC 3 cut(s) 253, 425, 1322
BceAI ACGGC 2 cut(s) 78, 982
BciT130I CCWGG 1 cut(s) 553
BclI TGATCA 5 cut(s) 220, 487, 1105, 1111, 1117
BcnI CCSGG 2 cut(s) 371, 392
BcuI ACTAGT 2 cut(s) 1139, 1145
BfaI CTAG 3 cut(s) 1140, 1146, 1151
BfmI CTRYAG 2 cut(s) 470, 647
BfuAI ACCTGC 2 cut(s) 477, 654
BglII AGATCT 1 cut(s) 304
BisI GCNGC 9 cut(s) 276, 321, 324, 408, 426, 508, 627, 814, 884
BlsI GCNGC 9 cut(s) 277, 322, 325, 409, 427, 509, 628, 815, 885
BmcAI AGTACT 1 cut(s) 1144
Bme1390I CCNGG 3 cut(s) 371, 392, 553
Bme18I GGWCC 1 cut(s) 764
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 764
BmiI GGNNCC 2 cut(s) 557, 882
BmrFI CCNGG 3 cut(s) 371, 392, 553
BmsI GCATC 1 cut(s) 1019
BpiI GAAGAC 1 cut(s) 535
BpmI CTGGAG 3 cut(s) 496, 574, 932
BpuMI CCSGG 2 cut(s) 371, 392
BsaBI GATNNNNATC 4 cut(s) 120, 225, 1110, 1116
BsaJI CCNNGG 3 cut(s) 315, 321, 573
BsaWI WCCGGW 3 cut(s) 620, 761, 845
Bsc4I CCNNNNNNNGG 1 cut(s) 773
Bse118I RCCGGY 1 cut(s) 819
Bse1I ACTGG 4 cut(s) 269, 767, 911, 1399
Bse3DI GCAATG 1 cut(s) 1156
Bse8I GATNNNNATC 4 cut(s) 120, 225, 1110, 1116
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 315, 321, 573
BseGI GGATG 3 cut(s) 94, 755, 1010
BseJI GATNNNNATC 4 cut(s) 120, 225, 1110, 1116
BseLI CCNNNNNNNGG 1 cut(s) 773
BseMI GCAATG 1 cut(s) 1156
BseMII CTCAG 5 cut(s) 249, 330, 461, 927, 1040
BseNI ACTGG 4 cut(s) 269, 767, 911, 1399
BseRI GAGGAG 2 cut(s) 42, 204
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 5 cut(s) 287, 419, 519, 638, 825
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 323
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 5 cut(s) 320, 503, 578, 823, 969
BshNI GGYRCC 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 820
BsiSI CCGG 7 cut(s) 371, 392, 621, 762, 774, 820, 846
BslFI GGGAC 3 cut(s) 362, 378, 622
BslI CCNNNNNNNGG 1 cut(s) 773
BsmFI GGGAC 3 cut(s) 362, 378, 622
BsnI GGCC 5 cut(s) 320, 503, 578, 823, 969
Bsp1286I GDGCHC 1 cut(s) 353
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 6 cut(s) 105, 321, 323, 326, 426, 884
BspANI GGCC 5 cut(s) 320, 503, 578, 823, 969
BspCNI CTCAG 5 cut(s) 250, 331, 460, 928, 1039
BspEI TCCGGA 1 cut(s) 620
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 3 cut(s) 217, 1108, 1114
BspLI GGNNCC 2 cut(s) 557, 882
BspMAI CTGCAG 2 cut(s) 474, 651
BspMI ACCTGC 2 cut(s) 477, 654
BspT107I GGYRCC 1 cut(s) 880
BsrDI GCAATG 1 cut(s) 1156
BsrFI RCCGGY 1 cut(s) 819
BsrI ACTGG 4 cut(s) 269, 767, 911, 1399
BssAI RCCGGY 1 cut(s) 819
BssECI CCNNGG 3 cut(s) 315, 321, 573
BssMI GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
BssT1I CCWWGG 2 cut(s) 315, 573
Bst2UI CCWGG 1 cut(s) 553
BstAPI GCANNNNNTGC 1 cut(s) 896
BstC8I GCNNGC 4 cut(s) 505, 651, 888, 1089
BstDEI CTNAG 6 cut(s) 193, 258, 339, 447, 936, 1026
BstDSI CCRYGG 2 cut(s) 321, 573
BstF5I GGATG 3 cut(s) 94, 755, 1010
BstFNI CGCG 1 cut(s) 323
BstKTI GATC 6 cut(s) 223, 307, 490, 1108, 1114, 1120
BstMBI GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
BstMCI CGRYCG 1 cut(s) 820
BstMWI GCNNNNNNNGC 6 cut(s) 71, 320, 329, 513, 632, 896
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 2 cut(s) 1091, 1330
BstSCI CCNGG 3 cut(s) 369, 390, 551
BstSFI CTRYAG 2 cut(s) 470, 647
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 5 cut(s) 287, 419, 519, 638, 825
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 5 cut(s) 320, 503, 578, 823, 969
BtgI CCRYGG 2 cut(s) 321, 573
BtrI CACGTC 1 cut(s) 358
BtsCI GGATG 3 cut(s) 94, 755, 1010
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 834, 918
BveI ACCTGC 2 cut(s) 477, 654
Cac8I GCNNGC 4 cut(s) 505, 651, 888, 1089
CaiI CAGNNNCTG 1 cut(s) 338
CciI TCATGA 3 cut(s) 217, 1108, 1114
Cfr10I RCCGGY 1 cut(s) 819
Cfr13I GGNCC 1 cut(s) 764
Cfr42I CCGCGG 1 cut(s) 324
CseI GACGC 1 cut(s) 1038
Csp6I GTAC 7 cut(s) 37, 266, 435, 466, 475, 943, 1143
CviQI GTAC 7 cut(s) 37, 266, 435, 466, 475, 943, 1143
DdeI CTNAG 6 cut(s) 193, 258, 339, 447, 936, 1026
DpnI GATC 6 cut(s) 222, 306, 489, 1107, 1113, 1119
DpnII GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
DrdI GACNNNNNNGTC 1 cut(s) 616
DseDI GACNNNNNNGTC 1 cut(s) 616
EaeI YGGCCR 3 cut(s) 576, 821, 967
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 315, 573
Eco32I GATATC 2 cut(s) 118, 241
Eco47I GGWCC 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 1409
EcoRII CCWGG 1 cut(s) 551
EcoRV GATATC 2 cut(s) 118, 241
EcoT14I CCWWGG 2 cut(s) 315, 573
ErhI CCWWGG 2 cut(s) 315, 573
FaqI GGGAC 3 cut(s) 362, 378, 622
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 1078
FbaI TGATCA 5 cut(s) 220, 487, 1105, 1111, 1117
Fnu4HI GCNGC 9 cut(s) 276, 321, 324, 408, 426, 508, 627, 814, 884
FokI GGATG 3 cut(s) 81, 742, 997
Fsp4HI GCNGC 9 cut(s) 276, 321, 324, 408, 426, 508, 627, 814, 884
FspBI CTAG 3 cut(s) 1140, 1146, 1151
GluI GCNGC 9 cut(s) 276, 321, 324, 408, 426, 508, 627, 814, 884
GsuI CTGGAG 3 cut(s) 496, 574, 932
HaeIII GGCC 5 cut(s) 320, 503, 578, 823, 969
HapII CCGG 7 cut(s) 371, 392, 621, 762, 774, 820, 846
HgaI GACGC 1 cut(s) 1038
HinfI GANTC 5 cut(s) 20, 569, 928, 1238, 1363
HpaII CCGG 7 cut(s) 371, 392, 621, 762, 774, 820, 846
HphI GGTGA 3 cut(s) 865, 928, 1144
Hpy166II GTNNAC 1 cut(s) 1214
Hpy188I TCNGA 6 cut(s) 259, 450, 933, 1029, 1362, 1404
Hpy188III TCNNGA 7 cut(s) 218, 621, 949, 1053, 1109, 1115, 1318
Hpy8I GTNNAC 1 cut(s) 1214
HpyAV CCTTC 2 cut(s) 852, 1120
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 5 cut(s) 472, 649, 813, 839, 1333
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 320, 329, 513, 632, 896
HpyF3I CTNAG 6 cut(s) 193, 258, 339, 447, 936, 1026
HpySE526I ACGT 1 cut(s) 357
Kpn2I TCCGGA 1 cut(s) 620
Ksp22I TGATCA 5 cut(s) 220, 487, 1105, 1111, 1117
KspI CCGCGG 1 cut(s) 324
Kzo9I GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
LmnI GCTCC 6 cut(s) 217, 515, 555, 623, 729, 861
Lsp1109I GCAGC 5 cut(s) 287, 419, 519, 638, 825
LweI GCATC 1 cut(s) 1019
MaeI CTAG 3 cut(s) 1140, 1146, 1151
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 853, 955, 1132
MalI GATC 6 cut(s) 222, 306, 489, 1107, 1113, 1119
MboI GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
MboII GAAGA 6 cut(s) 77, 125, 243, 314, 535, 649
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 5 cut(s) 997, 1189, 1304, 1409, 1423
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 3 cut(s) 956, 1090, 1399
Mox20I TGGCCA 1 cut(s) 578
MroI TCCGGA 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 1409
MscI TGGCCA 1 cut(s) 578
MseI TTAA 3 cut(s) 600, 662, 996
MslI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 2 cut(s) 323, 886
MspI CCGG 7 cut(s) 371, 392, 621, 762, 774, 820, 846
MspR9I CCNGG 3 cut(s) 371, 392, 553
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 6 cut(s) 71, 320, 329, 513, 632, 896
NciI CCSGG 2 cut(s) 371, 392
NcoI CCATGG 1 cut(s) 573
NdeI CATATG 1 cut(s) 1078
NdeII GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
NlaIV GGNNCC 2 cut(s) 557, 882
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 3 cut(s) 853, 955, 1132
NspI RCATGY 2 cut(s) 1091, 1330
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 3 cut(s) 217, 1108, 1114
PciI ACATGT 1 cut(s) 1326
PdmI GAANNNNTTC 1 cut(s) 1409
PfeI GAWTC 4 cut(s) 20, 928, 1238, 1363
PfoI TCCNGGA 1 cut(s) 551
PkrI GCNGC 9 cut(s) 277, 322, 325, 409, 427, 509, 628, 815, 885
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
PscI ACATGT 1 cut(s) 1326
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 2 cut(s) 557, 882
PspPI GGNCC 1 cut(s) 764
PstI CTGCAG 2 cut(s) 474, 651
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 7 cut(s) 38, 267, 436, 467, 476, 944, 1144
RsaNI GTAC 7 cut(s) 37, 266, 435, 466, 475, 943, 1143
RseI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
SacII CCGCGG 1 cut(s) 324
SaqAI TTAA 3 cut(s) 600, 662, 996
SatI GCNGC 9 cut(s) 276, 321, 324, 408, 426, 508, 627, 814, 884
Sau3AI GATC 6 cut(s) 220, 304, 487, 1105, 1111, 1117
Sau96I GGNCC 1 cut(s) 764
SbfI CCTGCAGG 1 cut(s) 651
ScaI AGTACT 1 cut(s) 1144
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 3 cut(s) 371, 392, 553
SdaI CCTGCAGG 1 cut(s) 651
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 1 cut(s) 1019
SfcI CTRYAG 2 cut(s) 470, 647
Sfr303I CCGCGG 1 cut(s) 324
SgrBI CCGCGG 1 cut(s) 324
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 3 cut(s) 1092, 1107, 1113
SpeI ACTAGT 2 cut(s) 1139, 1145
SphI GCATGC 1 cut(s) 1091
Sse8387I CCTGCAGG 1 cut(s) 651
Sse9I AATT 5 cut(s) 997, 1189, 1304, 1409, 1423
SsiI CCGC 6 cut(s) 105, 321, 323, 326, 426, 884
SspI AATATT 1 cut(s) 796
SspMI CTAG 3 cut(s) 1140, 1146, 1151
StyD4I CCNGG 3 cut(s) 369, 390, 551
StyI CCWWGG 2 cut(s) 315, 573
TaiI ACGT 1 cut(s) 360
TaqI TCGA 1 cut(s) 1366
TasI AATT 5 cut(s) 997, 1189, 1304, 1409, 1423
TatI WGTACW 1 cut(s) 1142
TauI GCSGC 4 cut(s) 323, 326, 428, 886
TfiI GAWTC 4 cut(s) 20, 928, 1238, 1363
Tru1I TTAA 3 cut(s) 600, 662, 996
Tru9I TTAA 3 cut(s) 600, 662, 996
TscAI CASTG 2 cut(s) 841, 918
TseFI GTSAC 3 cut(s) 853, 955, 1132
TseI GCWGC 5 cut(s) 275, 407, 507, 626, 813
Tsp45I GTSAC 3 cut(s) 853, 955, 1132
TspDTI ATGAA 9 cut(s) 17, 243, 656, 973, 1017, 1095, 1145, 1230, 1260
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 841, 918
VpaK11BI GGWCC 1 cut(s) 764
XapI RAATTY 2 cut(s) 1189, 1409
XceI RCATGY 2 cut(s) 1091, 1330
XmnI GAANNNNTTC 1 cut(s) 1409
XspI CTAG 3 cut(s) 1140, 1146, 1151
ZrmI AGTACT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.