pycom111g00380

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0004816
Physical Location & Seq
Forward (+)
278175 .. 279625
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00380.1

Sequence Viewer

Length: 921 bp
ATGAACCACCCCAACAACCAAGCATCCCAACCTCCACTATACCCCTCCATTTCTCCTCCCTCTCACCAAGACATAAACCCTTTCCTCCCAACTCCTCCGCCTGAAGATATCAACCCCTTTCATCAAACACCTGCGCCGATGCCACCGTCCTCGTCGCCGCAAACCCATTTCGAAACACCTCCTAGCGTATCCTCCCCTCAGAATGGGAAAACCGAAGAGCTAAAACGCACTACTCCTGATCAAGATGTTCCGTCGTCGTCAAGAGGCGCATCTCCTCCGTCAATCCCACCAACTCCAAGTGAGGAAGAAAAATCAGCGCAGTGGGGGACTCACGTCATGGGGCACCCTGCCGTCCCAACATGCCATCCGGACAACAAAAAAGCAGCTCTGTGGGGTGCTGCCGGAACCGAGCAAGCCCAGAATCCCTACCTCCAGTACAAACCCCTTGACAAGTCCAACAACACCAGTCCATTGGAATCCATGCTCCACGTCTTCAACTCCTGGAGCAACAAGGCCGAGTCCATGGCCAACAACATCTGGCACAACCTGAAAACGGGGACATCGGTGTCTGGAGCAGCGTGGGCGAAGATGAACGTGACGGCGAAAGCAATAACAGGAGGAGGATTTGAGGCTCTGTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTGTACAAGTCATTTGCCTGTTATCTGTCCACATCCACAGGACCAGTTGCCGGAACTCTTTACCTCTCTAATATCCACACAGCTTTCTGCAGCGACCGCCCTTTATCCTTCACCGCCCCCTCCGGCCAGGCGTCGAGGCATTTGACCCAGAGTATGTCAACATTTGTGGCATCTGGAGTTGCCGTCAAACCAATCGTTGCTGCAGACAATGACATTGCATACCAGAAGCCTGACAAGGCTGACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

307

Amino Acids

32.95

Weight (kDa)

6.59

Isoelectric Point (pI)

60.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 214 - 258 1.3e-06 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 4 cut(s) 98, 158, 769, 786
AcoI YGGCCR 2 cut(s) 525, 796
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 3 cut(s) 437, 638, 677
AfiI CCNNNNNNNGG 3 cut(s) 203, 553, 722
AgsI TTSAA 1 cut(s) 496
AjiI CACGTC 2 cut(s) 334, 490
AjnI CCWGG 2 cut(s) 500, 798
AluBI AGCT 4 cut(s) 220, 386, 673, 755
AluI AGCT 4 cut(s) 220, 386, 673, 755
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 3 cut(s) 513, 525, 796
ApeKI GCWGC 5 cut(s) 383, 398, 575, 762, 872
AspLEI GCGC 3 cut(s) 136, 269, 319
AspS9I GGNCC 1 cut(s) 713
AsuHPI GGTGA 2 cut(s) 56, 775
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 1 cut(s) 342
BbsI GAAGAC 1 cut(s) 484
BbvI GCAGC 5 cut(s) 385, 395, 587, 774, 859
BccI CCATC 1 cut(s) 372
BceAI ACGGC 3 cut(s) 335, 615, 839
BciT130I CCWGG 2 cut(s) 502, 800
BciVI GTATCC 1 cut(s) 199
BclI TGATCA 1 cut(s) 238
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 2 cut(s) 760, 873
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 199
BisI GCNGC 6 cut(s) 158, 384, 399, 576, 763, 873
BlsI GCNGC 6 cut(s) 159, 385, 400, 577, 764, 874
Bme1390I CCNGG 2 cut(s) 502, 800
Bme18I GGWCC 1 cut(s) 713
BmgBI CACGTC 2 cut(s) 334, 490
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 2 cut(s) 344, 406
BmrFI CCNGG 2 cut(s) 502, 800
BmsI GCATC 4 cut(s) 32, 129, 278, 851
BoxI GACNNNNGTC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 484
BpmI CTGGAG 4 cut(s) 416, 523, 591, 867
Bpu14I TTCGAA 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 803
BsaJI CCNNGG 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 553, 722
Bse1I ACTGG 3 cut(s) 433, 465, 716
Bse3DI GCAATG 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 2 cut(s) 502, 800
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 3 cut(s) 23, 364, 704
BseLI CCNNNNNNNGG 3 cut(s) 203, 553, 722
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 3 cut(s) 433, 465, 716
BseRI GAGGAG 4 cut(s) 45, 84, 264, 633
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 5 cut(s) 385, 395, 587, 774, 859
Bsh1285I CGRYCG 1 cut(s) 769
BshFI GGCC 3 cut(s) 515, 527, 798
BshNI GGYRCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 769
BsiSI CCGG 4 cut(s) 368, 402, 723, 795
BslFI GGGAC 3 cut(s) 338, 340, 571
BslI CCNNNNNNNGG 3 cut(s) 203, 553, 722
BsmFI GGGAC 3 cut(s) 338, 340, 571
BsnI GGCC 3 cut(s) 515, 527, 798
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 345
Bsp13I TCCGGA 1 cut(s) 367
Bsp1407I TGTACA 2 cut(s) 636, 675
Bsp143I GATC 1 cut(s) 238
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 4 cut(s) 98, 158, 769, 786
BspANI GGCC 3 cut(s) 515, 527, 798
BspCNI CTCAG 1 cut(s) 211
BspEI TCCGGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 344, 406
BspMAI CTGCAG 2 cut(s) 764, 877
BspMI ACCTGC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 885
BsrGI TGTACA 2 cut(s) 636, 675
BsrI ACTGG 3 cut(s) 433, 465, 716
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 803
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 502, 800
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 803
BstAUI TGTACA 2 cut(s) 636, 675
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 3 cut(s) 23, 364, 704
BstHHI GCGC 3 cut(s) 136, 269, 319
BstKTI GATC 1 cut(s) 241
BstMBI GATC 1 cut(s) 238
BstMCI CGRYCG 1 cut(s) 769
BstMWI GCNNNNNNNGC 2 cut(s) 581, 768
BstNI CCWGG 2 cut(s) 502, 800
BstNSI RCATGY 1 cut(s) 363
BstPAI GACNNNNGTC 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 500, 798
BstSFI CTRYAG 2 cut(s) 760, 873
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 5 cut(s) 385, 395, 587, 774, 859
BstV2I GAAGAC 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 3 cut(s) 515, 527, 798
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 2 cut(s) 334, 490
BtsCI GGATG 3 cut(s) 23, 364, 704
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 1 cut(s) 326
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 3 cut(s) 136, 269, 319
Cfr13I GGNCC 1 cut(s) 713
CseI GACGC 1 cut(s) 792
Csp6I GTAC 3 cut(s) 436, 637, 676
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 4 cut(s) 337, 360, 481, 523
CviQI GTAC 3 cut(s) 436, 637, 676
DdeI CTNAG 1 cut(s) 198
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 2 cut(s) 525, 796
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 522
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 713
Eco57I CTGAAG 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 500, 798
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 4 cut(s) 340, 363, 484, 526
FaiI YATR 8 cut(s) 40, 74, 338, 361, 482, 524, 827, 892
FaqI GGGAC 3 cut(s) 338, 340, 571
FatI CATG 4 cut(s) 336, 359, 480, 522
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 6 cut(s) 158, 384, 399, 576, 763, 873
FokI GGATG 3 cut(s) 10, 351, 691
Fsp4HI GCNGC 6 cut(s) 158, 384, 399, 576, 763, 873
FspBI CTAG 1 cut(s) 183
GlaI GCGC 3 cut(s) 135, 268, 318
GluI GCNGC 6 cut(s) 158, 384, 399, 576, 763, 873
GsuI CTGGAG 4 cut(s) 416, 523, 591, 867
HaeIII GGCC 3 cut(s) 515, 527, 798
HapII CCGG 4 cut(s) 368, 402, 723, 795
HgaI GACGC 1 cut(s) 792
HhaI GCGC 3 cut(s) 136, 269, 319
Hin1I GRCGYC 1 cut(s) 803
Hin1II CATG 4 cut(s) 340, 363, 484, 526
Hin6I GCGC 3 cut(s) 134, 267, 317
HinP1I GCGC 3 cut(s) 134, 267, 317
HincII GTYRAC 1 cut(s) 831
HindII GTYRAC 1 cut(s) 831
HinfI GANTC 4 cut(s) 328, 421, 476, 518
HpaII CCGG 4 cut(s) 368, 402, 723, 795
HphI GGTGA 2 cut(s) 56, 775
Hpy166II GTNNAC 2 cut(s) 702, 831
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 6 cut(s) 236, 242, 261, 368, 570, 846
Hpy8I GTNNAC 2 cut(s) 702, 831
Hpy99I CGWCG 4 cut(s) 157, 256, 259, 808
HpyAV CCTTC 1 cut(s) 790
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 3 cut(s) 333, 489, 594
HpyCH4V TGCA 3 cut(s) 762, 875, 890
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 768
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 3 cut(s) 333, 489, 594
Hsp92I GRCGYC 1 cut(s) 803
Hsp92II CATG 4 cut(s) 340, 363, 484, 526
HspAI GCGC 3 cut(s) 134, 267, 317
Kpn2I TCCGGA 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 1 cut(s) 238
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 3 cut(s) 489, 504, 572
Lsp1109I GCAGC 5 cut(s) 385, 395, 587, 774, 859
LweI GCATC 4 cut(s) 32, 129, 278, 851
MaeI CTAG 1 cut(s) 183
MaeII ACGT 3 cut(s) 333, 489, 594
MaeIII GTNAC 1 cut(s) 595
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 5 cut(s) 116, 227, 317, 484, 598
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 527
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 2 cut(s) 322, 527
MmeI TCCRAC 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 527
MroI TCCGGA 1 cut(s) 367
MscI TGGCCA 1 cut(s) 527
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 4 cut(s) 368, 402, 723, 795
MspR9I CCNGG 2 cut(s) 502, 800
MvaI CCWGG 2 cut(s) 502, 800
MwoI GCNNNNNNNGC 2 cut(s) 581, 768
NcoI CCATGG 1 cut(s) 522
NdeII GATC 1 cut(s) 238
NlaIII CATG 4 cut(s) 340, 363, 484, 526
NlaIV GGNNCC 2 cut(s) 344, 406
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 363
NspV TTCGAA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 210
PfeI GAWTC 2 cut(s) 421, 476
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 6 cut(s) 159, 385, 400, 577, 764, 874
PleI GAGTC 2 cut(s) 322, 526
PpsI GAGTC 2 cut(s) 322, 526
PshAI GACNNNNGTC 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 500, 798
PspGI CCWGG 2 cut(s) 500, 798
PspN4I GGNNCC 2 cut(s) 344, 406
PspPI GGNCC 1 cut(s) 713
PstI CTGCAG 2 cut(s) 764, 877
RsaI GTAC 3 cut(s) 437, 638, 677
RsaNI GTAC 3 cut(s) 436, 637, 676
SapI GCTCTTC 1 cut(s) 210
SatI GCNGC 6 cut(s) 158, 384, 399, 576, 763, 873
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 2 cut(s) 322, 527
ScrFI CCNGG 2 cut(s) 502, 800
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 4 cut(s) 32, 129, 278, 851
SfcI CTRYAG 2 cut(s) 760, 873
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 713
SsiI CCGC 4 cut(s) 98, 158, 769, 786
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 500, 798
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 3 cut(s) 336, 492, 597
TaqI TCGA 2 cut(s) 171, 806
TatI WGTACW 3 cut(s) 435, 636, 675
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 2 cut(s) 421, 476
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 5 cut(s) 383, 398, 575, 762, 872
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 17, 110, 605
TspGWI ACGGA 2 cut(s) 240, 267
TspRI CASTG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 713
XceI RCATGY 1 cut(s) 363
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.