AT5G16140

Chloroplastic group IIB intron splicing facilitator CRS2-B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003153
Physical Location & Seq
Reverse (-)
5270160 .. 5271963
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16140.2

Sequence Viewer

Length: 723 bp
ATGTTGTGCCCAACGTATACACCAAGTGTTTACCATTTTCAAACGCAAAAGCCAGTTTTCCGGAAACAGAGGTTTCGGGTTTGTTCATCTACTTCTGGTGGTGAGGATGATAGATTCAAAGTAGAGTACACTCCTTGGCTTATCGTTGGGTTGGGCAATCCAGGAAACAAGTATCATGGAACAAGACACAATGTTGGTTTCGAGATGATCGATGTGTTGGCTAGAAAAGAAGGGGTGTTGATGAACACTATACAGTCCAAAGCTCTAATCGGAATAGGTGCTATTGAAGATGTACCTATCTTGTTGGCTAAACCTCAGACTTATATGAACTTCAGCGGCGAATCGGTTGGATCTCTAGCTTCACACTATCGAGTACCATTGCGCCACATTTTAATGATTTACGATGAGATGGCTTTACCAAATGGGGTTTTGAGACTTCAACCGAAAGGAGGTCAAGGCTATCACAACGGAGTGAAGAGTGTAATGGGGCATTTGGATGGTCGTCGAAACTTTCCTCGTTTAAGCATAGGCATTGGTAAACCACCTGGAAACATGGATATGAAAGCTTTTCTTCTTCAGAAGTTTAGTCCATTGGAACAGAAACAGATCGAGGAAGCACTCGAGCAAGGAAGTGAAGCAGTGAAGACTCTTGTGCTTAATGGCTTCAATCAAGGAATTTCGAGATTTAATCTTGTGCAGAAATATAAATTCCATAAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

240

Amino Acids

26.93

Weight (kDa)

9.67

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 47 - 230 1.2e-59 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 17
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 1 cut(s) 336
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 2 cut(s) 675, 707
AcuI CTGAAG 2 cut(s) 316, 560
AdeI CACNNNGTG 1 cut(s) 26
AfaI GTAC 3 cut(s) 128, 294, 375
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 5 cut(s) 41, 118, 287, 440, 667
AjnI CCWGG 2 cut(s) 160, 544
AluBI AGCT 3 cut(s) 263, 359, 566
AluI AGCT 3 cut(s) 263, 359, 566
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 1 cut(s) 358
Ama87I CYCGRG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 60
ApoI RAATTY 2 cut(s) 675, 707
AspLEI GCGC 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 620
BaeGI GKGCMC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 650
BccI CCATC 2 cut(s) 403, 491
BciT130I CCWGG 2 cut(s) 162, 546
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 2 cut(s) 222, 356
BisI GCNGC 1 cut(s) 337
BlsI GCNGC 1 cut(s) 338
Bme1390I CCNGG 2 cut(s) 162, 546
BmeT110I CYCGRG 1 cut(s) 620
BmrFI CCNGG 2 cut(s) 162, 546
BpiI GAAGAC 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 377
BseAI TCCGGA 1 cut(s) 60
BseBI CCWGG 2 cut(s) 162, 546
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 2 cut(s) 112, 502
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 716
BshVI ATCGAT 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 427
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 11
Bsp13I TCCGGA 1 cut(s) 60
Bsp143I GATC 3 cut(s) 207, 350, 606
BspACI CCGC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 328
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 1 cut(s) 60
BspPI GGATC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 3 cut(s) 207, 350, 606
BssNAI GTATAC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 134
Bst1107I GTATAC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 162, 546
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 470
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 112, 502
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 3 cut(s) 210, 353, 609
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 3 cut(s) 207, 350, 606
BstNI CCWGG 2 cut(s) 162, 546
BstSCI CCNGG 2 cut(s) 160, 544
BstSLI GKGCMC 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BstZ17I GTATAC 1 cut(s) 18
Bsu15I ATCGAT 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 2 cut(s) 112, 502
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 1 cut(s) 645
CfoI GCGC 1 cut(s) 384
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 3 cut(s) 127, 293, 374
CviAII CATG 2 cut(s) 176, 553
CviQI GTAC 3 cut(s) 127, 293, 374
DdeI CTNAG 1 cut(s) 315
DpnI GATC 3 cut(s) 209, 352, 608
DpnII GATC 3 cut(s) 207, 350, 606
DraIII CACNNNGTG 1 cut(s) 26
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 134
Eco57I CTGAAG 2 cut(s) 316, 560
Eco88I CYCGRG 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 160, 544
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 2 cut(s) 179, 556
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FatI CATG 2 cut(s) 175, 552
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 1 cut(s) 337
FokI GGATG 2 cut(s) 119, 509
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 2 cut(s) 222, 356
GlaI GCGC 1 cut(s) 383
GluI GCNGC 1 cut(s) 337
HapII CCGG 1 cut(s) 61
HhaI GCGC 1 cut(s) 384
Hin1II CATG 2 cut(s) 179, 556
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 564
HinfI GANTC 3 cut(s) 114, 341, 646
HpaII CCGG 1 cut(s) 61
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 4 cut(s) 18, 31, 129, 539
Hpy188I TCNGA 3 cut(s) 272, 318, 579
Hpy188III TCNNGA 3 cut(s) 61, 202, 681
Hpy8I GTNNAC 4 cut(s) 18, 31, 129, 539
Hpy99I CGWCG 1 cut(s) 507
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 1 cut(s) 697
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 179, 556
HspAI GCGC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 207, 350, 606
LpnPI CCDG 7 cut(s) 66, 74, 81, 147, 174, 531, 558
MaeI CTAG 2 cut(s) 222, 356
MaeII ACGT 1 cut(s) 14
MalI GATC 3 cut(s) 209, 352, 608
MboI GATC 3 cut(s) 207, 350, 606
MboII GAAGA 5 cut(s) 299, 487, 563, 566, 655
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 2 cut(s) 675, 707
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 6 cut(s) 63, 97, 324, 443, 525, 604
MroI TCCGGA 1 cut(s) 60
MseI TTAA 4 cut(s) 392, 521, 657, 687
MslI CAYNNNNRTG 3 cut(s) 392, 495, 557
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 162, 546
MvaI CCWGG 2 cut(s) 162, 546
NdeII GATC 3 cut(s) 207, 350, 606
NlaIII CATG 2 cut(s) 179, 556
PaeR7I CTCGAG 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 114, 341
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 1 cut(s) 338
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
Psp6I CCWGG 2 cut(s) 160, 544
PspGI CCWGG 2 cut(s) 160, 544
PspXI VCTCGAGB 1 cut(s) 620
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 3 cut(s) 128, 294, 375
RsaNI GTAC 3 cut(s) 127, 293, 374
RseI CAYNNNNRTG 3 cut(s) 392, 495, 557
SaqAI TTAA 4 cut(s) 392, 521, 657, 687
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 3 cut(s) 207, 350, 606
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 2 cut(s) 162, 546
SduI GDGCHC 1 cut(s) 11
Sfr274I CTCGAG 1 cut(s) 620
SlaI CTCGAG 1 cut(s) 620
SmiMI CAYNNNNRTG 3 cut(s) 392, 495, 557
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 2 cut(s) 675, 707
SsiI CCGC 1 cut(s) 336
SspMI CTAG 2 cut(s) 222, 356
StyD4I CCNGG 2 cut(s) 160, 544
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 17
TaqI TCGA 7 cut(s) 201, 210, 370, 505, 609, 621, 680
TasI AATT 2 cut(s) 675, 707
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 2 cut(s) 114, 341
Tru1I TTAA 4 cut(s) 392, 521, 657, 687
Tru9I TTAA 4 cut(s) 392, 521, 657, 687
TscAI CASTG 1 cut(s) 645
TspDTI ATGAA 4 cut(s) 75, 257, 341, 575
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 1 cut(s) 645
XapI RAATTY 2 cut(s) 675, 707
XhoI CTCGAG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 2 cut(s) 222, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.