Rh2CG270800

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003153
Physical Location & Seq
Forward (+)
29491644 .. 29525003
33360 bp
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UTR
Exon/CDS
Intron
Rh2CG270800.1

Sequence Viewer

Length: 606 bp
ATGATTTTTGGATTGGGCAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTAGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGACGCAGTTCATGATGGAAACCATAACAGCTCTCATTGAGAAGTATGTTGGAGACACCCTGAATGAAGCTCATTATGTTAATGCCTTCAAAAGAGCAAGAGGAGATTCTCCGAAGCATGGAAGTTGGGCTGGCTTTCAAGGACTACGTTACTTGACAGAAAATATTGAATTTGAAAATCTACTTCACAAAACTTCAGTGTCTTTTTGTGCAACTCGAGTTGCTTCTTCAGGCCATGGTGGGTATTCGTATCTCTTGGAGCCTCTGTGCTGGTTGGGATTGATTACAAACTACTCAACTCTTGTAACGAACAAGCAGTATGTGTTTTATTCCAACATTGGTAGAGTAGGACATAAGTTAGAAGCTGCTATTTATCGATATTACCGGCAAAGTAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

201

Amino Acids

22.38

Weight (kDa)

8.97

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 1 - 78 2.9e-22 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 154
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 2 cut(s) 384, 417
AfaI GTAC 2 cut(s) 35, 156
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 5 cut(s) 65, 295, 344, 374, 380
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 5 cut(s) 182, 236, 275, 569, 600
AluI AGCT 5 cut(s) 182, 236, 275, 569, 600
Alw26I GTCTC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 1 cut(s) 569
ApoI RAATTY 1 cut(s) 374
Asp700I GAANNNNTTC 1 cut(s) 139
Asp718I GGTACC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 420
BanI GGYRCC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 556
BccI CCATC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 45
BisI GCNGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 420
BmiI GGNNCC 3 cut(s) 142, 156, 465
BmrFI CCNGG 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 22, 37, 439
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 24
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 22, 37, 439
BseLI CCNNNNNNNGG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 556
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 1 cut(s) 154
BshVI ATCGAT 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 1 cut(s) 589
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 252
BsnI GGCC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 420
Bsp19I CCATGG 2 cut(s) 37, 439
BspANI GGCC 1 cut(s) 438
BspDI ATCGAT 1 cut(s) 580
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 3 cut(s) 142, 156, 465
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 588
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 3 cut(s) 22, 37, 439
BssT1I CCWWGG 2 cut(s) 37, 439
Bst2UI CCWGG 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 337
BstDSI CCRYGG 2 cut(s) 37, 439
BstMAI GTCTC 1 cut(s) 252
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 580
BsuRI GGCC 1 cut(s) 438
BsuTUI ATCGAT 1 cut(s) 580
BtgI CCRYGG 2 cut(s) 37, 439
BtsIMutI CAGTG 2 cut(s) 125, 408
Cac8I GCNNGC 1 cut(s) 337
CciI TCATGA 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 588
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 1 cut(s) 217
Csp6I GTAC 2 cut(s) 34, 155
CviAII CATG 5 cut(s) 38, 187, 217, 323, 440
CviQI GTAC 2 cut(s) 34, 155
Eco130I CCWWGG 2 cut(s) 37, 439
Eco57I CTGAAG 2 cut(s) 384, 417
Eco88I CYCGRG 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 2 cut(s) 37, 439
ErhI CCWWGG 2 cut(s) 37, 439
FaeI CATG 5 cut(s) 41, 190, 220, 326, 443
FalI AAGNNNNNCTT 1 cut(s) 586
FatI CATG 5 cut(s) 37, 186, 216, 322, 439
Fnu4HI GCNGC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 570
FspBI CTAG 1 cut(s) 45
GluI GCNGC 1 cut(s) 570
HaeIII GGCC 1 cut(s) 438
HapII CCGG 1 cut(s) 589
HgaI GACGC 1 cut(s) 217
Hin1II CATG 5 cut(s) 41, 190, 220, 326, 443
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 1 cut(s) 311
HpaII CCGG 1 cut(s) 589
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 2 cut(s) 217, 603
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 301
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 1 cut(s) 416
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 5 cut(s) 41, 190, 220, 326, 443
KpnI GGTACC 1 cut(s) 158
LmnI GCTCC 1 cut(s) 463
LpnPI CCDG 9 cut(s) 9, 36, 77, 113, 278, 321, 420, 460, 602
Lsp1109I GCAGC 1 cut(s) 556
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 2 cut(s) 353, 508
MboII GAAGA 1 cut(s) 423
MluCI AATT 4 cut(s) 111, 159, 190, 374
MmeI TCCRAC 3 cut(s) 112, 235, 561
MnlI CCTC 4 cut(s) 145, 186, 299, 477
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 24
NcoI CCATGG 2 cut(s) 37, 439
NlaIII CATG 5 cut(s) 41, 190, 220, 326, 443
NlaIV GGNNCC 3 cut(s) 142, 156, 465
PaeR7I CTCGAG 1 cut(s) 420
PagI TCATGA 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 3 cut(s) 142, 156, 465
PspXI VCTCGAGB 1 cut(s) 420
RsaI GTAC 2 cut(s) 35, 156
RsaNI GTAC 2 cut(s) 34, 155
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 570
ScrFI CCNGG 1 cut(s) 24
SetI ASST 8 cut(s) 142, 156, 184, 238, 277, 355, 571, 602
Sfr274I CTCGAG 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 177, 420
SmoI CTYRAG 2 cut(s) 177, 420
Sse9I AATT 4 cut(s) 111, 159, 190, 374
SspI AATATT 1 cut(s) 370
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 2 cut(s) 37, 439
TaiI ACGT 1 cut(s) 355
TaqI TCGA 2 cut(s) 421, 580
TasI AATT 4 cut(s) 111, 159, 190, 374
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 132, 408
TseI GCWGC 1 cut(s) 569
TspDTI ATGAA 4 cut(s) 119, 203, 205, 285
TspRI CASTG 2 cut(s) 132, 408
XapI RAATTY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 420
XmnI GAANNNNTTC 1 cut(s) 139
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.