Rh5BG133000

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003153
Physical Location & Seq
Reverse (-)
13162462 .. 13185326
22865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG133000.1

Sequence Viewer

Length: 873 bp
ATGGCTGCTGTGAGGTTGAACGCAAAGATTTTCACCGACGGCTACTTCTCCTTGTACCCCTGCAAGCTCGAAGAGGTCAACATCAAGTGCGGCGAGATCTCAAAATTTACAGATCTCAAACTTGGCAGCTGTTCCAGTTTCACGCTCCACCTTCTTCATTTTATACATCGAGCGGTCTCTCTCTTTTCCTCTGCGACTAGTCTTCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTCGGTACCCTACCCGGTAGAACTGGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGCCGGAGAATAGCGATAGATTTAAAGTGGAATACACTCCATGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAATGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGACGCAGTTCATGATGGAAACCATGACAGCTCTCATTGAGAAGGGAAAGAAGCTGGGAGAAGAGAAGGATCTCATGGAAAAGGAGTTCATAATATATGCAGTTTATATTATGCATGTGAGTGGAGTTGTGGTATCCTGGTTCCAATTTAACATTAATTTTTTGGTCTATCTACTGAGGCGCATTTGTGCTACAGTAGTAGCAAACAATGCAAAACATGAGGAACAAAACTTTGCTGCAACTAAGGAACACAAGCTACTCCTTGAGACAAACAAAGCTATTTCCAACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

290

Amino Acids

32.29

Weight (kDa)

8.19

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 126 - 202 2.3e-22 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 260, 529
AccB1I GGYRCC 2 cut(s) 260, 529
AccB7I CCANNNNNTGG 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 173
AciI CCGC 3 cut(s) 90, 173, 230
AclWI GGATC 1 cut(s) 657
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 5 cut(s) 56, 262, 321, 410, 531
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 3 cut(s) 19, 306, 440
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 2 cut(s) 397, 716
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 2 cut(s) 181, 839
AlwI GGATC 1 cut(s) 657
ApeKI GCWGC 3 cut(s) 5, 126, 815
ApoI RAATTY 1 cut(s) 104
AseI ATTAAT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 514
Asp718I GGTACC 2 cut(s) 260, 529
AspLEI GCGC 1 cut(s) 762
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 3 cut(s) 25, 219, 556
BanI GGYRCC 2 cut(s) 260, 529
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 2 cut(s) 138, 802
BccI CCATC 1 cut(s) 589
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 2 cut(s) 399, 718
BciVI GTATCC 1 cut(s) 724
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 2 cut(s) 181, 839
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 420
BfmI CTRYAG 1 cut(s) 771
BfuI GTATCC 1 cut(s) 724
BglII AGATCT 2 cut(s) 96, 112
BisI GCNGC 4 cut(s) 6, 91, 127, 816
BlsI GCNGC 4 cut(s) 7, 92, 128, 817
Bme1390I CCNGG 3 cut(s) 270, 399, 718
BmiI GGNNCC 4 cut(s) 262, 517, 531, 722
BmrFI CCNGG 3 cut(s) 270, 399, 718
BpiI GAAGAC 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 537, 863
BpuMI CCSGG 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 3 cut(s) 371, 397, 412
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse1I ACTGG 3 cut(s) 135, 283, 507
Bse3DI GCAATG 1 cut(s) 233
BseBI CCWGG 2 cut(s) 399, 718
BseDI CCNNGG 3 cut(s) 371, 397, 412
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 3 cut(s) 135, 283, 507
BseXI GCAGC 2 cut(s) 138, 802
BseYI CCCAGC 1 cut(s) 634
BshNI GGYRCC 2 cut(s) 260, 529
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 2 cut(s) 270, 335
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 2 cut(s) 181, 839
Bso31I GGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 3 cut(s) 96, 112, 649
Bsp19I CCATGG 2 cut(s) 371, 412
BspACI CCGC 3 cut(s) 90, 173, 230
BspCNI CTCAG 1 cut(s) 747
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 4 cut(s) 262, 517, 531, 722
BspPI GGATC 1 cut(s) 657
BspT107I GGYRCC 2 cut(s) 260, 529
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 319
BsrI ACTGG 3 cut(s) 135, 283, 507
BssECI CCNNGG 3 cut(s) 371, 397, 412
BssMI GATC 3 cut(s) 96, 112, 649
BssT1I CCWWGG 2 cut(s) 371, 412
Bst2UI CCWGG 2 cut(s) 399, 718
Bst4CI ACNGT 1 cut(s) 775
Bst6I CTCTTC 2 cut(s) 66, 636
BstAPI GCANNNNNTGC 1 cut(s) 788
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 65, 221
BstDEI CTNAG 2 cut(s) 755, 822
BstDSI CCRYGG 2 cut(s) 371, 412
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 762
BstKTI GATC 3 cut(s) 99, 115, 652
BstMAI GTCTC 2 cut(s) 181, 839
BstMBI GATC 3 cut(s) 96, 112, 649
BstMWI GCNNNNNNNGC 2 cut(s) 229, 788
BstNI CCWGG 2 cut(s) 399, 718
BstNSI RCATGY 1 cut(s) 698
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 268, 397, 716
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 2 cut(s) 138, 802
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 3 cut(s) 96, 112, 649
BstYI RGATCY 3 cut(s) 96, 112, 649
BsuI GTATCC 1 cut(s) 724
BtgI CCRYGG 2 cut(s) 371, 412
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 65, 221
CciI TCATGA 1 cut(s) 591
CfoI GCGC 1 cut(s) 762
CseI GACGC 1 cut(s) 592
Csp6I GTAC 5 cut(s) 55, 261, 320, 409, 530
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviAII CATG 8 cut(s) 372, 413, 562, 592, 604, 655, 695, 797
CviQI GTAC 5 cut(s) 55, 261, 320, 409, 530
DdeI CTNAG 2 cut(s) 755, 822
DpnI GATC 3 cut(s) 98, 114, 651
DpnII GATC 3 cut(s) 96, 112, 649
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 66, 636
EarI CTCTTC 2 cut(s) 66, 636
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 371, 412
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 219
EcoO65I GGTNACC 1 cut(s) 392
EcoRII CCWGG 2 cut(s) 397, 716
EcoT14I CCWWGG 2 cut(s) 371, 412
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 371, 412
FaeI CATG 8 cut(s) 375, 416, 565, 595, 607, 658, 698, 800
FatI CATG 8 cut(s) 371, 412, 561, 591, 603, 654, 694, 796
Fnu4HI GCNGC 4 cut(s) 6, 91, 127, 816
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 4 cut(s) 6, 91, 127, 816
FspBI CTAG 2 cut(s) 198, 420
GlaI GCGC 1 cut(s) 761
GluI GCNGC 4 cut(s) 6, 91, 127, 816
GsaI CCCAGC 1 cut(s) 638
HapII CCGG 2 cut(s) 270, 335
HgaI GACGC 1 cut(s) 592
HhaI GCGC 1 cut(s) 762
Hin1II CATG 8 cut(s) 375, 416, 565, 595, 607, 658, 698, 800
Hin6I GCGC 1 cut(s) 760
HinP1I GCGC 1 cut(s) 760
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 324, 555
HpaII CCGG 2 cut(s) 270, 335
HphI GGTGA 3 cut(s) 25, 219, 556
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 1 cut(s) 592
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 41, 586
HpyAV CCTTC 3 cut(s) 161, 616, 640
HpyCH4III ACNGT 1 cut(s) 775
HpyCH4V TGCA 6 cut(s) 63, 466, 680, 694, 791, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 788
HpyF3I CTNAG 2 cut(s) 755, 822
Hsp92II CATG 8 cut(s) 375, 416, 565, 595, 607, 658, 698, 800
HspAI GCGC 1 cut(s) 760
KpnI GGTACC 2 cut(s) 264, 533
Kzo9I GATC 3 cut(s) 96, 112, 649
LmnI GCTCC 2 cut(s) 150, 216
Lsp1109I GCAGC 2 cut(s) 138, 802
MaeI CTAG 2 cut(s) 198, 420
MaeIII GTNAC 2 cut(s) 225, 392
MalI GATC 3 cut(s) 98, 114, 651
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 96, 112, 649
MboII GAAGA 5 cut(s) 83, 146, 194, 260, 653
MflI RGATCY 3 cut(s) 96, 112, 649
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 6 cut(s) 104, 486, 534, 565, 725, 736
MmeI TCCRAC 2 cut(s) 364, 487
MnlI CCTC 8 cut(s) 6, 67, 199, 249, 520, 561, 750, 793
Mph1103I ATGCAT 1 cut(s) 696
MroXI GAANNNNTTC 1 cut(s) 514
MseI TTAA 3 cut(s) 354, 729, 735
MslI CAYNNNNRTG 2 cut(s) 476, 699
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 2 cut(s) 270, 335
MspR9I CCNGG 3 cut(s) 270, 399, 718
MvaI CCWGG 2 cut(s) 399, 718
MwoI GCNNNNNNNGC 2 cut(s) 229, 788
NciI CCSGG 1 cut(s) 270
NcoI CCATGG 2 cut(s) 371, 412
NdeII GATC 3 cut(s) 96, 112, 649
NlaIII CATG 8 cut(s) 375, 416, 565, 595, 607, 658, 698, 800
NlaIV GGNNCC 4 cut(s) 262, 517, 531, 722
NmuCI GTSAC 1 cut(s) 225
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 1 cut(s) 698
PagI TCATGA 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 514
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 4 cut(s) 7, 92, 128, 817
PshBI ATTAAT 1 cut(s) 735
Psp124BI GAGCTC 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 397, 716
PspEI GGTNACC 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 2 cut(s) 397, 716
PspN4I GGNNCC 4 cut(s) 262, 517, 531, 722
PsrI GAACNNNNNNTAC 2 cut(s) 819, 851
PsuI RGATCY 3 cut(s) 96, 112, 649
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 5 cut(s) 56, 262, 321, 410, 531
RsaNI GTAC 5 cut(s) 55, 261, 320, 409, 530
RseI CAYNNNNRTG 2 cut(s) 476, 699
SacI GAGCTC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 354, 729, 735
SatI GCNGC 4 cut(s) 6, 91, 127, 816
Sau3AI GATC 3 cut(s) 96, 112, 649
ScrFI CCNGG 3 cut(s) 270, 399, 718
SduI GDGCHC 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 2 cut(s) 476, 699
SmlI CTYRAG 2 cut(s) 552, 842
SmoI CTYRAG 2 cut(s) 552, 842
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 6 cut(s) 104, 486, 534, 565, 725, 736
SsiI CCGC 3 cut(s) 90, 173, 230
SspMI CTAG 2 cut(s) 198, 420
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 3 cut(s) 268, 397, 716
StyI CCWWGG 2 cut(s) 371, 412
TaaI ACNGT 1 cut(s) 775
TaqI TCGA 3 cut(s) 69, 169, 241
TasI AATT 6 cut(s) 104, 486, 534, 565, 725, 736
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 93
Tru1I TTAA 3 cut(s) 354, 729, 735
Tru9I TTAA 3 cut(s) 354, 729, 735
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 3 cut(s) 5, 126, 815
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 5 cut(s) 146, 494, 578, 580, 658
TspRI CASTG 1 cut(s) 507
Van91I CCANNNNNTGG 1 cut(s) 213
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 1 cut(s) 104
XceI RCATGY 1 cut(s) 698
XmnI GAANNNNTTC 1 cut(s) 514
XspI CTAG 2 cut(s) 198, 420
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.