FvH4_5g29670

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003153
Physical Location & Seq
Reverse (-)
20600462 .. 20604761
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29670.t4

Sequence Viewer

Length: 828 bp
ATGGCAGCAGTCTCTCTCTTTTCCTCTGCTACTAGTCTCCCCACCATTTGGAGCTCGCTGTCACCGCCATTGCCTCGAGCCCAAGAAGCTCTTGGTACCCTGTCAATGTTTGATGCTGTGTCGTATCCACGAAGACCGGGTAGAACTCGGTTTCGGACAAAGTGTTCAGTTTCAGCAAGTGTTTCTGTACAAGCTCCCTTGCCGGAGAATGAGGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCGGGAAATAAGTACCATGGAACTCGGCACAATGTTGGTTTTGAGATGATAGATACTATTTCCAAAACACAAGGGATTGTGATGAATACAATTCAATCAAAGGCATTGGTTGGAATAGGTTCTATTGGAGAGGTGCCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAGTTACAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACGTCATATCTTATTGGTTTATGATGAGATGAGCCTACCAAATGGTGTTCTAAGATTGCAGCCGAAAGCAGGGCATGGCTATCACAATGGTGTGAAGAGTGTAGTGGGCCATCTGGATGGCTGCCGTGATTTTCCTCGCTTATGTATAGGTATTGGAAATCCACCAGGAACTATGGACATGAAAGCGTATCTTCTACAAAAATTTAGTACAGTAGAAAGACATCAGATTGATGCAGCATTGGGTCAAGGCGTTGAGGCTGTGAAGACACTGGTGCTGAATGGATTTGACCCGAATATCACTAGTTTCAATTTGAGGCAAAAGTACAAGCATCACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

276

Amino Acids

30.08

Weight (kDa)

9.47

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 82 - 265 9.9e-54 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 95, 397
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 2 cut(s) 65, 449
AcsI RAATTY 1 cut(s) 686
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 6 cut(s) 97, 189, 278, 480, 694, 809
AfiI CCNNNNNNNGG 2 cut(s) 48, 554
AgsI TTSAA 2 cut(s) 359, 793
AhlI ACTAGT 2 cut(s) 32, 785
AjnI CCWGG 1 cut(s) 649
AleI CACNNNNGTG 1 cut(s) 573
AloI GAACNNNNNNTCC 2 cut(s) 148, 180
AluBI AGCT 4 cut(s) 54, 89, 194, 425
AluI AGCT 4 cut(s) 54, 89, 194, 425
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 16, 41
Ama87I CYCGRG 2 cut(s) 75, 265
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 5 cut(s) 5, 464, 544, 606, 719
ApoI RAATTY 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 382
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 455, 592
AsuC2I CCSGG 3 cut(s) 138, 266, 267
AsuHPI GGTGA 2 cut(s) 54, 424
AvaI CYCGRG 2 cut(s) 75, 265
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 95, 397
BanII GRGCYC 2 cut(s) 56, 82
BbsI GAAGAC 2 cut(s) 139, 755
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 5 cut(s) 17, 476, 556, 593, 731
BccI CCATC 2 cut(s) 596, 603
BceAI ACGGC 1 cut(s) 594
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 135
BcnI CCSGG 3 cut(s) 138, 266, 267
BcoDI GTCTC 2 cut(s) 16, 41
BcuI ACTAGT 2 cut(s) 32, 785
BfaI CTAG 2 cut(s) 33, 786
BfuI GTATCC 1 cut(s) 135
BisI GCNGC 5 cut(s) 6, 465, 545, 607, 720
BlsI GCNGC 5 cut(s) 7, 466, 546, 608, 721
Bme1390I CCNGG 4 cut(s) 138, 266, 267, 651
Bme18I GGWCC 1 cut(s) 455
BmeT110I CYCGRG 2 cut(s) 75, 265
BmgT120I GGNCC 2 cut(s) 455, 592
BmiI GGNNCC 2 cut(s) 97, 399
BmrFI CCNGG 4 cut(s) 138, 266, 267, 651
BmsI GCATC 3 cut(s) 103, 706, 823
BpiI GAAGAC 2 cut(s) 139, 755
BpuEI CTTGAG 1 cut(s) 405
BpuMI CCSGG 3 cut(s) 138, 266, 267
BsaJI CCNNGG 3 cut(s) 239, 265, 280
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 554
Bse1I ACTGG 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 239, 265, 280
BseGI GGATG 2 cut(s) 249, 607
BseLI CCNNNNNNNGG 2 cut(s) 48, 554
BseMI GCAATG 1 cut(s) 68
BseNI ACTGG 1 cut(s) 759
BseXI GCAGC 5 cut(s) 17, 476, 556, 593, 731
BshFI GGCC 1 cut(s) 594
BshNI GGYRCC 2 cut(s) 95, 397
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 2 cut(s) 75, 265
BsiSI CCGG 3 cut(s) 137, 203, 266
BslI CCNNNNNNNGG 2 cut(s) 48, 554
BsmAI GTCTC 2 cut(s) 16, 41
BsnI GGCC 1 cut(s) 594
BsoBI CYCGRG 2 cut(s) 75, 265
Bsp1286I GDGCHC 2 cut(s) 56, 82
Bsp1407I TGTACA 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 2 cut(s) 65, 449
BspANI GGCC 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 97, 399
BspT107I GGYRCC 2 cut(s) 95, 397
BsrDI GCAATG 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 187
BsrI ACTGG 1 cut(s) 759
BssECI CCNNGG 3 cut(s) 239, 265, 280
BssT1I CCWWGG 2 cut(s) 239, 280
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 2 cut(s) 440, 697
Bst6I CTCTTC 1 cut(s) 575
BstAUI TGTACA 1 cut(s) 187
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 536
BstDSI CCRYGG 1 cut(s) 280
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 2 cut(s) 249, 607
BstMAI GTCTC 2 cut(s) 16, 41
BstMWI GCNNNNNNNGC 2 cut(s) 64, 86
BstNI CCWGG 1 cut(s) 651
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 136, 264, 265, 649
BstV1I GCAGC 5 cut(s) 17, 476, 556, 593, 731
BstV2I GAAGAC 2 cut(s) 139, 755
BstXI CCANNNNNNTGG 1 cut(s) 602
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 1 cut(s) 594
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 249, 607
BtsIMutI CAGTG 2 cut(s) 445, 752
Cac8I GCNNGC 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 455, 592
Cfr9I CCCGGG 1 cut(s) 265
Csp6I GTAC 6 cut(s) 96, 188, 277, 479, 693, 808
CspCI CAANNNNNGTGG 2 cut(s) 390, 425
CviAII CATG 4 cut(s) 281, 430, 560, 664
CviQI GTAC 6 cut(s) 96, 188, 277, 479, 693, 808
DdeI CTNAG 1 cut(s) 536
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 823
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 239, 280
Eco24I GRGCYC 2 cut(s) 56, 82
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 75, 265
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 239, 280
EcoT38I GRGCYC 2 cut(s) 56, 82
ErhI CCWWGG 2 cut(s) 239, 280
FaeI CATG 4 cut(s) 284, 433, 563, 667
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 660, 692
FatI CATG 4 cut(s) 280, 429, 559, 663
Fnu4HI GCNGC 5 cut(s) 6, 465, 545, 607, 720
FokI GGATG 2 cut(s) 256, 614
FriOI GRGCYC 2 cut(s) 56, 82
Fsp4HI GCNGC 5 cut(s) 6, 465, 545, 607, 720
FspBI CTAG 2 cut(s) 33, 786
GluI GCNGC 5 cut(s) 6, 465, 545, 607, 720
HaeIII GGCC 1 cut(s) 594
HapII CCGG 3 cut(s) 137, 203, 266
Hin1II CATG 4 cut(s) 284, 433, 563, 667
HindIII AAGCTT 1 cut(s) 423
HpaII CCGG 3 cut(s) 137, 203, 266
HphI GGTGA 2 cut(s) 54, 424
Hpy188I TCNGA 2 cut(s) 156, 711
Hpy188III TCNNGA 1 cut(s) 599
HpyCH4III ACNGT 2 cut(s) 440, 697
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 3 cut(s) 464, 544, 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 86
HpyF3I CTNAG 1 cut(s) 536
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 4 cut(s) 284, 433, 563, 667
KpnI GGTACC 1 cut(s) 99
LmnI GCTCC 2 cut(s) 51, 199
LpnPI CCDG 9 cut(s) 113, 150, 216, 279, 540, 584, 636, 663, 740
Lsp1109I GCAGC 5 cut(s) 17, 476, 556, 593, 731
LweI GCATC 3 cut(s) 103, 706, 823
MaeI CTAG 2 cut(s) 33, 786
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 3 cut(s) 60, 260, 434
MboII GAAGA 4 cut(s) 144, 592, 668, 760
MhlI GDGCHC 2 cut(s) 56, 82
MluCI AATT 4 cut(s) 354, 402, 686, 793
MmeI TCCRAC 3 cut(s) 232, 355, 433
MnlI CCTC 9 cut(s) 34, 84, 205, 388, 429, 492, 630, 733, 792
MroXI GAANNNNTTC 1 cut(s) 382
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 2 cut(s) 573, 600
MspI CCGG 3 cut(s) 137, 203, 266
MspR9I CCNGG 4 cut(s) 138, 266, 267, 651
MvaI CCWGG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 64, 86
NciI CCSGG 3 cut(s) 138, 266, 267
NcoI CCATGG 1 cut(s) 280
NlaIII CATG 4 cut(s) 284, 433, 563, 667
NlaIV GGNNCC 2 cut(s) 97, 399
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 573
PaeR7I CTCGAG 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 5 cut(s) 7, 466, 546, 608, 721
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 649
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 2 cut(s) 97, 399
PspPI GGNCC 2 cut(s) 455, 592
PspXI VCTCGAGB 1 cut(s) 75
RsaI GTAC 6 cut(s) 97, 189, 278, 480, 694, 809
RsaNI GTAC 6 cut(s) 96, 188, 277, 479, 693, 808
RseI CAYNNNNRTG 2 cut(s) 573, 600
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 5 cut(s) 6, 465, 545, 607, 720
Sau96I GGNCC 2 cut(s) 455, 592
ScrFI CCNGG 4 cut(s) 138, 266, 267, 651
SduI GDGCHC 2 cut(s) 56, 82
SetI ASST 9 cut(s) 56, 91, 196, 385, 399, 427, 484, 490, 637
SfaNI GCATC 3 cut(s) 103, 706, 823
Sfr274I CTCGAG 1 cut(s) 75
SinI GGWCC 1 cut(s) 455
SlaI CTCGAG 1 cut(s) 75
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 573, 600
SmlI CTYRAG 2 cut(s) 75, 420
SmoI CTYRAG 2 cut(s) 75, 420
SpeI ACTAGT 2 cut(s) 32, 785
Sse9I AATT 4 cut(s) 354, 402, 686, 793
SsiI CCGC 2 cut(s) 65, 449
SspMI CTAG 2 cut(s) 33, 786
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 4 cut(s) 136, 264, 265, 649
StyI CCWWGG 2 cut(s) 239, 280
TaaI ACNGT 2 cut(s) 440, 697
TaiI ACGT 1 cut(s) 490
TaqI TCGA 1 cut(s) 76
TasI AATT 4 cut(s) 354, 402, 686, 793
TatI WGTACW 3 cut(s) 187, 692, 807
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 2 cut(s) 445, 759
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 5 cut(s) 5, 464, 544, 606, 719
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 362, 680
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 2 cut(s) 445, 759
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 686
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 75
XmaI CCCGGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 382
XspI CTAG 2 cut(s) 33, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.