Rh4BG080600

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003153
Physical Location & Seq
Forward (+)
14059823 .. 14066226
6404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG080600.1

Sequence Viewer

Length: 813 bp
ATGGCTGCTGTGAGGTTGAACGCAAAGATTTTCACCGACGGCTACTTCTCCTTGTACCCCTGCAAGCTCGAAGAGGTCAACATCAAGTGCGGCGAGATCTCAAAATTTACAGATCTCAAACCCGGCAGCTGTTCCAGTTTCACGCTCCACCTTCTTCATTTTATACATCGAGCGGTCTCTCTCTTTTCCTCTGCGACTAGTCTTCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTTGGTACCCTACCCGGTAGAACTCGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGTCGGAGAATAGCGATAAATTTAAAGTGGAATACACTCCATGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAATGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGACGCAGTTCATGATGGAAACCATGACAGCTCTCATTGAGAAGGGAAACAAGCTGGGAGAAGAGAAGGATCTCATGGAAAAGGAGCACAAACTTTCCACCGAGGTGGATGATGATTCGGAATTGAGCACTGGAGTTAGTATTCCCAAAGTCAGCGACGGTGGATTTGCTAACACGATGTCGATGGCGTTGGTTTTCTCAACCCCGAATCAACTCTGGATCCCAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

270

Amino Acids

29.64

Weight (kDa)

6.09

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 126 - 202 2e-22 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 260, 529
AccB1I GGYRCC 2 cut(s) 260, 529
AccB7I CCANNNNNTGG 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 173
AciI CCGC 3 cut(s) 90, 173, 230
AclWI GGATC 3 cut(s) 657, 793, 806
AcsI RAATTY 2 cut(s) 104, 350
AfaI GTAC 5 cut(s) 56, 262, 321, 410, 531
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 3 cut(s) 19, 306, 440
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 397
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AleI CACNNNNGTG 1 cut(s) 683
AluBI AGCT 8 cut(s) 67, 129, 219, 254, 326, 557, 611, 634
AluI AGCT 8 cut(s) 67, 129, 219, 254, 326, 557, 611, 634
Alw21I GWGCWC 3 cut(s) 221, 669, 710
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 3 cut(s) 657, 793, 806
ApeKI GCWGC 2 cut(s) 5, 126
ApoI RAATTY 2 cut(s) 104, 350
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
Asp700I GAANNNNTTC 1 cut(s) 514
Asp718I GGTACC 2 cut(s) 260, 529
AsuC2I CCSGG 2 cut(s) 123, 270
AsuHPI GGTGA 3 cut(s) 25, 219, 556
BamHI GGATCC 1 cut(s) 798
BanI GGYRCC 2 cut(s) 260, 529
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 194, 799
Bbv12I GWGCWC 3 cut(s) 221, 669, 710
BbvI GCAGC 1 cut(s) 138
BccI CCATC 2 cut(s) 589, 757
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 399
BcnI CCSGG 2 cut(s) 123, 270
BcoDI GTCTC 1 cut(s) 181
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 3 cut(s) 198, 420, 811
BglII AGATCT 2 cut(s) 96, 112
BisI GCNGC 3 cut(s) 6, 91, 127
BlsI GCNGC 3 cut(s) 7, 92, 128
Bme1390I CCNGG 3 cut(s) 123, 270, 399
BmiI GGNNCC 4 cut(s) 262, 517, 531, 800
BmrFI CCNGG 3 cut(s) 123, 270, 399
BmrI ACTGGG 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 797
BpiI GAAGAC 2 cut(s) 194, 799
BpmI CTGGAG 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 537
BpuMI CCSGG 2 cut(s) 123, 270
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 4 cut(s) 371, 397, 412, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse1I ACTGG 4 cut(s) 135, 507, 715, 803
Bse3DI GCAATG 1 cut(s) 233
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 4 cut(s) 371, 397, 412, 681
BseGI GGATG 2 cut(s) 381, 694
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 233
BseNI ACTGG 4 cut(s) 135, 507, 715, 803
BseXI GCAGC 1 cut(s) 138
BseYI CCCAGC 1 cut(s) 634
BshNI GGYRCC 2 cut(s) 260, 529
BsiHKAI GWGCWC 3 cut(s) 221, 669, 710
BsiSI CCGG 2 cut(s) 123, 270
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 3 cut(s) 221, 669, 710
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 4 cut(s) 96, 112, 649, 798
Bsp19I CCATGG 2 cut(s) 371, 412
BspACI CCGC 3 cut(s) 90, 173, 230
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 4 cut(s) 262, 517, 531, 800
BspPI GGATC 3 cut(s) 657, 793, 806
BspT107I GGYRCC 2 cut(s) 260, 529
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 319
BsrI ACTGG 4 cut(s) 135, 507, 715, 803
BssECI CCNNGG 4 cut(s) 371, 397, 412, 681
BssMI GATC 4 cut(s) 96, 112, 649, 798
BssT1I CCWWGG 2 cut(s) 371, 412
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 740
Bst6I CTCTTC 2 cut(s) 66, 636
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 65, 221
BstDSI CCRYGG 2 cut(s) 371, 412
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 2 cut(s) 381, 694
BstKTI GATC 4 cut(s) 99, 115, 652, 801
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 4 cut(s) 96, 112, 649, 798
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNI CCWGG 1 cut(s) 399
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 121, 268, 397
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 2 cut(s) 194, 799
BstX2I RGATCY 4 cut(s) 96, 112, 649, 798
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 4 cut(s) 96, 112, 649, 798
BtgI CCRYGG 2 cut(s) 371, 412
BtsCI GGATG 2 cut(s) 381, 694
BtsIMutI CAGTG 2 cut(s) 500, 708
Cac8I GCNNGC 2 cut(s) 65, 221
CciI TCATGA 1 cut(s) 591
CseI GACGC 1 cut(s) 592
Csp6I GTAC 5 cut(s) 55, 261, 320, 409, 530
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviAII CATG 6 cut(s) 372, 413, 562, 592, 604, 655
CviQI GTAC 5 cut(s) 55, 261, 320, 409, 530
DpnI GATC 4 cut(s) 98, 114, 651, 800
DpnII GATC 4 cut(s) 96, 112, 649, 798
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 66, 636
EarI CTCTTC 2 cut(s) 66, 636
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 371, 412
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 219
EcoO65I GGTNACC 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 371, 412
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 371, 412
FaeI CATG 6 cut(s) 375, 416, 565, 595, 607, 658
FaiI YATR 8 cut(s) 164, 373, 414, 563, 570, 593, 605, 656
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 6 cut(s) 371, 412, 561, 591, 603, 654
Fnu4HI GCNGC 3 cut(s) 6, 91, 127
FokI GGATG 2 cut(s) 388, 701
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 6, 91, 127
FspBI CTAG 3 cut(s) 198, 420, 811
GluI GCNGC 3 cut(s) 6, 91, 127
GsaI CCCAGC 1 cut(s) 638
GsuI CTGGAG 1 cut(s) 732
HapII CCGG 2 cut(s) 123, 270
HgaI GACGC 1 cut(s) 592
Hin1II CATG 6 cut(s) 375, 416, 565, 595, 607, 658
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 324, 555
HinfI GANTC 2 cut(s) 695, 787
HpaII CCGG 2 cut(s) 123, 270
HphI GGTGA 3 cut(s) 25, 219, 556
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 288, 337, 700
Hpy188III TCNNGA 2 cut(s) 592, 796
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 3 cut(s) 41, 586, 740
HpyAV CCTTC 3 cut(s) 161, 616, 640
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4V TGCA 2 cut(s) 63, 466
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
Hsp92II CATG 6 cut(s) 375, 416, 565, 595, 607, 658
KpnI GGTACC 2 cut(s) 264, 533
Kzo9I GATC 4 cut(s) 96, 112, 649, 798
LmnI GCTCC 3 cut(s) 150, 216, 664
Lsp1109I GCAGC 1 cut(s) 138
MaeI CTAG 3 cut(s) 198, 420, 811
MaeIII GTNAC 2 cut(s) 225, 392
MalI GATC 4 cut(s) 98, 114, 651, 800
MbiI CCGCTC 1 cut(s) 173
MboI GATC 4 cut(s) 96, 112, 649, 798
MboII GAAGA 6 cut(s) 83, 146, 194, 260, 653, 799
MflI RGATCY 4 cut(s) 96, 112, 649, 798
MhlI GDGCHC 3 cut(s) 221, 669, 710
MluCI AATT 6 cut(s) 104, 350, 486, 534, 565, 701
MmeI TCCRAC 3 cut(s) 315, 364, 487
MnlI CCTC 7 cut(s) 6, 67, 199, 249, 520, 561, 676
MroXI GAANNNNTTC 1 cut(s) 514
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 2 cut(s) 476, 683
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 2 cut(s) 123, 270
MspR9I CCNGG 3 cut(s) 123, 270, 399
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 229
NciI CCSGG 2 cut(s) 123, 270
NcoI CCATGG 2 cut(s) 371, 412
NdeII GATC 4 cut(s) 96, 112, 649, 798
NlaIII CATG 6 cut(s) 375, 416, 565, 595, 607, 658
NlaIV GGNNCC 4 cut(s) 262, 517, 531, 800
NmuCI GTSAC 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 683
PagI TCATGA 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 514
PfeI GAWTC 2 cut(s) 695, 787
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 3 cut(s) 7, 92, 128
Psp124BI GAGCTC 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 397
PspEI GGTNACC 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 4 cut(s) 262, 517, 531, 800
PsuI RGATCY 4 cut(s) 96, 112, 649, 798
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 5 cut(s) 56, 262, 321, 410, 531
RsaNI GTAC 5 cut(s) 55, 261, 320, 409, 530
RseI CAYNNNNRTG 2 cut(s) 476, 683
SacI GAGCTC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 3 cut(s) 6, 91, 127
Sau3AI GATC 4 cut(s) 96, 112, 649, 798
ScrFI CCNGG 3 cut(s) 123, 270, 399
SduI GDGCHC 3 cut(s) 221, 669, 710
SmiMI CAYNNNNRTG 2 cut(s) 476, 683
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 6 cut(s) 104, 350, 486, 534, 565, 701
SsiI CCGC 3 cut(s) 90, 173, 230
SspMI CTAG 3 cut(s) 198, 420, 811
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 3 cut(s) 121, 268, 397
StyI CCWWGG 2 cut(s) 371, 412
TaaI ACNGT 1 cut(s) 740
TaqI TCGA 4 cut(s) 69, 169, 241, 761
TasI AATT 6 cut(s) 104, 350, 486, 534, 565, 701
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 2 cut(s) 695, 787
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 2 cut(s) 507, 715
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 2 cut(s) 5, 126
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 4 cut(s) 146, 494, 578, 580
TspRI CASTG 2 cut(s) 507, 715
Van91I CCANNNNNTGG 1 cut(s) 213
XapI RAATTY 2 cut(s) 104, 350
XmnI GAANNNNTTC 1 cut(s) 514
XspI CTAG 3 cut(s) 198, 420, 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.