RchiOBHm_Chr5g0050971

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003153
Physical Location & Seq
Forward (+)
51823332 .. 51823839
508 bp
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UTR
Exon/CDS
Intron
PRQ32849

Sequence Viewer

Length: 234 bp
ATGTGCTATGCAGAGACTAAGCTTTTTCATGTGGTTTTAGCATCTCAGGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTTAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCAGTGTGTTCTCATCATCTTTGTTTGCTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

77

Amino Acids

8.33

Weight (kDa)

6.23

Isoelectric Point (pI)

19.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 16 - 69 1.9e-09 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 148
AfaI GTAC 1 cut(s) 150
AgsI TTSAA 1 cut(s) 59
AluBI AGCT 2 cut(s) 22, 176
AluI AGCT 2 cut(s) 22, 176
Alw26I GTCTC 1 cut(s) 8
Asp700I GAANNNNTTC 1 cut(s) 133
Asp718I GGTACC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 175
BanI GGYRCC 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 136, 150
BmsI GCATC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 136, 150
BspT107I GGYRCC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 18, 45
BstMAI GTCTC 1 cut(s) 8
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 119, 207
Csp6I GTAC 1 cut(s) 149
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 2 cut(s) 29, 181
CviJI RGCY 3 cut(s) 22, 169, 176
CviKI_1 RGCY 3 cut(s) 22, 169, 176
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 2 cut(s) 18, 45
FaeI CATG 2 cut(s) 32, 184
FaiI YATR 4 cut(s) 9, 30, 182, 189
FatI CATG 2 cut(s) 28, 180
Hin1II CATG 2 cut(s) 32, 184
HindIII AAGCTT 2 cut(s) 20, 174
HphI GGTGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 18, 45
Hsp92II CATG 2 cut(s) 32, 184
KpnI GGTACC 1 cut(s) 152
LpnPI CCDG 3 cut(s) 32, 71, 107
LweI GCATC 1 cut(s) 50
MluCI AATT 3 cut(s) 105, 153, 184
MmeI TCCRAC 1 cut(s) 106
MnlI CCTC 2 cut(s) 139, 180
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 1 cut(s) 95
NlaIII CATG 2 cut(s) 32, 184
NlaIV GGNNCC 2 cut(s) 136, 150
PdmI GAANNNNTTC 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 136, 150
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 113
SetI ASST 5 cut(s) 24, 51, 136, 150, 178
SfaNI GCATC 1 cut(s) 50
SgeI CNNG 6 cut(s) 41, 59, 98, 134, 185, 193
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 3 cut(s) 105, 153, 184
TasI AATT 3 cut(s) 105, 153, 184
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 2 cut(s) 126, 207
TspDTI ATGAA 3 cut(s) 17, 113, 197
TspRI CASTG 2 cut(s) 126, 207
XmnI GAANNNNTTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.