Rh5BG347100

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003153
Physical Location & Seq
Forward (+)
53021176 .. 53023418
2243 bp
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UTR
Exon/CDS
Intron
Rh5BG347100.1

Sequence Viewer

Length: 318 bp
ATGTCCCAATCAATAAGAACATTTTTTGACATAGTGATTGTTGGATTGGGTAACCCTAGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTTAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCAACGCAGTTCACGATGGAAACCATAATAGCTCTCATTGAGAAGATGCAGGCTCAATTTTGTTATACTGAAGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

105

Amino Acids

11.7

Weight (kDa)

5.58

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 12 - 87 8.4e-22 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 187
AfaI GTAC 2 cut(s) 68, 189
AgsI TTSAA 1 cut(s) 98
AluBI AGCT 2 cut(s) 215, 269
AluI AGCT 2 cut(s) 215, 269
Asp700I GAANNNNTTC 1 cut(s) 172
Asp718I GGTACC 1 cut(s) 187
AsuHPI GGTGA 1 cut(s) 214
BanI GGYRCC 1 cut(s) 187
BccI CCATC 1 cut(s) 247
BfaI CTAG 3 cut(s) 57, 78, 316
BmiI GGNNCC 2 cut(s) 175, 189
BmsI GCATC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 175, 189
BspT107I GGYRCC 1 cut(s) 187
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 70
BstEII GGTNACC 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 67, 188
CspCI CAANNNNNGTGG 2 cut(s) 180, 215
CviAII CATG 2 cut(s) 71, 220
CviJI RGCY 4 cut(s) 208, 215, 269, 290
CviKI_1 RGCY 4 cut(s) 208, 215, 269, 290
CviQI GTAC 2 cut(s) 67, 188
Eco130I CCWWGG 1 cut(s) 70
Eco91I GGTNACC 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 74, 223
FaiI YATR 6 cut(s) 32, 72, 221, 228, 263, 303
FatI CATG 2 cut(s) 70, 219
FspBI CTAG 3 cut(s) 57, 78, 316
Hin1II CATG 2 cut(s) 74, 223
HindIII AAGCTT 1 cut(s) 213
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 249
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 1 cut(s) 249
HpyCH4V TGCA 1 cut(s) 286
Hsp92II CATG 2 cut(s) 74, 223
KpnI GGTACC 1 cut(s) 191
LpnPI CCDG 3 cut(s) 110, 146, 272
LweI GCATC 1 cut(s) 273
MaeI CTAG 3 cut(s) 57, 78, 316
MaeIII GTNAC 1 cut(s) 50
MboII GAAGA 1 cut(s) 292
MluCI AATT 4 cut(s) 144, 192, 223, 293
MmeI TCCRAC 2 cut(s) 22, 145
MnlI CCTC 2 cut(s) 178, 219
MroXI GAANNNNTTC 1 cut(s) 172
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 134
NcoI CCATGG 1 cut(s) 70
NlaIII CATG 2 cut(s) 74, 223
NlaIV GGNNCC 2 cut(s) 175, 189
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 172
PspEI GGTNACC 1 cut(s) 50
PspN4I GGNNCC 2 cut(s) 175, 189
RsaI GTAC 2 cut(s) 68, 189
RsaNI GTAC 2 cut(s) 67, 188
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 1 cut(s) 152
SetI ASST 4 cut(s) 175, 189, 217, 271
SfaNI GCATC 1 cut(s) 273
SgeI CNNG 9 cut(s) 69, 83, 90, 137, 173, 224, 232, 262, 299
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 4 cut(s) 144, 192, 223, 293
SspMI CTAG 3 cut(s) 57, 78, 316
StyI CCWWGG 1 cut(s) 70
TasI AATT 4 cut(s) 144, 192, 223, 293
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TscAI CASTG 1 cut(s) 165
TspDTI ATGAA 2 cut(s) 152, 236
TspRI CASTG 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 172
XspI CTAG 3 cut(s) 57, 78, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.