Rw7G030410

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003153
Physical Location & Seq
Reverse (-)
43164754 .. 43168389
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030410.1

Sequence Viewer

Length: 798 bp
ATGGCAGCAATCTCTCTCTTTTCCTCTGCGACTAGTCTCCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTCGGTACCCTACCCGTTAGAACTCGTTTTCTGAACAAGTGTTCGGTTTCAACAAGTTTTTCAGTACAAGCTTCCTCGCCGGAGAATAATAGCGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCACGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGTTGAATACAATTCAGTCAAAGTCGCTGGTTGGAATAGGTTCAATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACGTCATATATTATTGGTTTATGATGAGATGAGCCTACCAAATGGTGTTTTAAGATTGCAGCCAAAAGGAGGACATGGCTATCACAATGGTGTGAAGAGTGTAGTGGGCCATCTGGATGGCTGCCGTGATTTTCCTCGCTTATGTATTGGTATTGGAAATCCACCTGGTACTATGGACATGAAAGCCTATCTTCTACAAAAATTCAGTACAGTAGAAAGACATCAGATTGATGCAGCACTGGGTCAAGGCGTTGAGGCTGTGAAGACACTGGTGCTAAATGGATTTGACCCGAATATCACTAGTTTCAATTTGAGGCAAAAATACAAGTACCACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

265

Amino Acids

28.96

Weight (kDa)

9.28

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 72 - 255 4.8e-56 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 95, 367
AccB1I GGYRCC 2 cut(s) 95, 367
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 2 cut(s) 65, 419
AcsI RAATTY 1 cut(s) 656
AdeI CACNNNGTG 1 cut(s) 793
AfaI GTAC 8 cut(s) 97, 156, 248, 369, 450, 625, 664, 785
AfiI CCNNNNNNNGG 2 cut(s) 48, 524
AgsI TTSAA 5 cut(s) 141, 278, 319, 357, 763
AhlI ACTAGT 2 cut(s) 32, 755
AjnI CCWGG 2 cut(s) 235, 619
AleI CACNNNNGTG 1 cut(s) 543
AluBI AGCT 4 cut(s) 54, 89, 161, 395
AluI AGCT 4 cut(s) 54, 89, 161, 395
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 41
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 5 cut(s) 5, 434, 514, 576, 689
ApoI RAATTY 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 352
Asp718I GGTACC 2 cut(s) 95, 367
AspS9I GGNCC 2 cut(s) 425, 562
AsuHPI GGTGA 2 cut(s) 54, 394
AvaII GGWCC 1 cut(s) 425
BanI GGYRCC 2 cut(s) 95, 367
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 5 cut(s) 17, 446, 526, 563, 701
BccI CCATC 2 cut(s) 566, 573
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 2 cut(s) 237, 621
BcoDI GTCTC 1 cut(s) 41
BcuI ACTAGT 2 cut(s) 32, 755
BfaI CTAG 3 cut(s) 33, 258, 756
BisI GCNGC 5 cut(s) 6, 435, 515, 577, 690
BlsI GCNGC 5 cut(s) 7, 436, 516, 578, 691
Bme1390I CCNGG 2 cut(s) 237, 621
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 425, 562
BmiI GGNNCC 2 cut(s) 97, 369
BmrFI CCNGG 2 cut(s) 237, 621
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 1 cut(s) 676
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 375
BsaJI CCNNGG 3 cut(s) 209, 235, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 524
Bse1I ACTGG 2 cut(s) 699, 729
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 237, 621
BseDI CCNNGG 3 cut(s) 209, 235, 250
BseGI GGATG 2 cut(s) 219, 577
BseLI CCNNNNNNNGG 2 cut(s) 48, 524
BseMI GCAATG 1 cut(s) 68
BseNI ACTGG 2 cut(s) 699, 729
BseXI GCAGC 5 cut(s) 17, 446, 526, 563, 701
BshFI GGCC 1 cut(s) 564
BshNI GGYRCC 2 cut(s) 95, 367
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 48, 524
BsmAI GTCTC 1 cut(s) 41
BsnI GGCC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 56
BspACI CCGC 2 cut(s) 65, 419
BspANI GGCC 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 97, 369
BspT107I GGYRCC 2 cut(s) 95, 367
BsrDI GCAATG 1 cut(s) 68
BsrI ACTGG 2 cut(s) 699, 729
BssECI CCNNGG 3 cut(s) 209, 235, 250
BssT1I CCWWGG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 237, 621
Bst4CI ACNGT 1 cut(s) 667
Bst6I CTCTTC 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 250
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 2 cut(s) 219, 577
BstMAI GTCTC 1 cut(s) 41
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 2 cut(s) 237, 621
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 235, 619
BstV1I GCAGC 5 cut(s) 17, 446, 526, 563, 701
BstV2I GAAGAC 1 cut(s) 725
BstXI CCANNNNNNTGG 1 cut(s) 572
BsuRI GGCC 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 219, 577
BtsIMutI CAGTG 2 cut(s) 692, 722
Cac8I GCNNGC 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 425, 562
CsiI ACCWGGT 1 cut(s) 619
Csp6I GTAC 8 cut(s) 96, 155, 247, 368, 449, 624, 663, 784
CspCI CAANNNNNGTGG 2 cut(s) 360, 395
CviAII CATG 3 cut(s) 400, 530, 634
CviQI GTAC 8 cut(s) 96, 155, 247, 368, 449, 624, 663, 784
DraI TTTAAA 1 cut(s) 193
DraIII CACNNNGTG 1 cut(s) 793
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 235, 619
EcoT14I CCWWGG 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 3 cut(s) 403, 533, 637
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FatI CATG 3 cut(s) 399, 529, 633
Fnu4HI GCNGC 5 cut(s) 6, 435, 515, 577, 690
FokI GGATG 2 cut(s) 226, 584
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 5 cut(s) 6, 435, 515, 577, 690
FspBI CTAG 3 cut(s) 33, 258, 756
GluI GCNGC 5 cut(s) 6, 435, 515, 577, 690
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 170
Hin1II CATG 3 cut(s) 403, 533, 637
HindIII AAGCTT 2 cut(s) 159, 393
HpaII CCGG 1 cut(s) 170
HphI GGTGA 2 cut(s) 54, 394
Hpy188I TCNGA 2 cut(s) 123, 681
Hpy188III TCNNGA 1 cut(s) 569
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 3 cut(s) 434, 514, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 3 cut(s) 403, 533, 637
KpnI GGTACC 2 cut(s) 99, 371
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 5 cut(s) 17, 446, 526, 563, 701
LweI GCATC 1 cut(s) 676
MabI ACCWGGT 1 cut(s) 619
MaeI CTAG 3 cut(s) 33, 258, 756
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 2 cut(s) 60, 230
MboII GAAGA 4 cut(s) 95, 562, 638, 730
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 6 cut(s) 324, 357, 372, 403, 656, 763
MmeI TCCRAC 3 cut(s) 202, 325, 403
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 2 cut(s) 192, 506
MslI CAYNNNNRTG 2 cut(s) 543, 570
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 2 cut(s) 237, 621
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 2 cut(s) 237, 621
MwoI GCNNNNNNNGC 1 cut(s) 64
NlaIII CATG 3 cut(s) 403, 533, 637
NlaIV GGNNCC 2 cut(s) 97, 369
NmuCI GTSAC 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 352
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 5 cut(s) 7, 436, 516, 578, 691
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 235, 619
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 235, 619
PspN4I GGNNCC 2 cut(s) 97, 369
PspPI GGNCC 2 cut(s) 425, 562
RsaI GTAC 8 cut(s) 97, 156, 248, 369, 450, 625, 664, 785
RsaNI GTAC 8 cut(s) 96, 155, 247, 368, 449, 624, 663, 784
RseI CAYNNNNRTG 2 cut(s) 543, 570
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 2 cut(s) 192, 506
SatI GCNGC 5 cut(s) 6, 435, 515, 577, 690
Sau96I GGNCC 2 cut(s) 425, 562
ScrFI CCNGG 2 cut(s) 237, 621
SduI GDGCHC 1 cut(s) 56
SetI ASST 9 cut(s) 56, 91, 163, 355, 369, 397, 454, 460, 622
SexAI ACCWGGT 1 cut(s) 619
SfaNI GCATC 1 cut(s) 676
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 543, 570
SmlI CTYRAG 1 cut(s) 390
SmoI CTYRAG 1 cut(s) 390
SpeI ACTAGT 2 cut(s) 32, 755
Sse9I AATT 6 cut(s) 324, 357, 372, 403, 656, 763
SsiI CCGC 2 cut(s) 65, 419
SspMI CTAG 3 cut(s) 33, 258, 756
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 235, 619
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 1 cut(s) 460
TaqI TCGA 1 cut(s) 76
TasI AATT 6 cut(s) 324, 357, 372, 403, 656, 763
TatI WGTACW 2 cut(s) 154, 662
Tru1I TTAA 2 cut(s) 192, 506
Tru9I TTAA 2 cut(s) 192, 506
TscAI CASTG 2 cut(s) 699, 729
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 5 cut(s) 5, 434, 514, 576, 689
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 416, 650
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 2 cut(s) 699, 729
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 3 cut(s) 33, 258, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.