Rh7AG357600

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003153
Physical Location & Seq
Reverse (-)
45849938 .. 45854216
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG357600.1

Sequence Viewer

Length: 579 bp
ATGGCAGCAGTCTCTCTCTTTTCCTCTGCGACTAGTCTCCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTTGGTACCCTACCCGGTAGAACTCGTTTTCTGAACAAGTGTTCAGTTTCAACAAGTTTTTCAGTACAAGCTTCCTTGCCGGAGAATAATAGCGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCACGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAGACGCAGGGCATTGTGATGAATACAATTCAGTCAAAGTCGCTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACGTCATATATTATTGGTTTATGATGAGATGAGCCTACCAAATGGTGTTTTAAGATTGCAGCCAAAAGGAGGACATGGCTATCACAATGGGTATGTAACTATGTATGAACAGTACTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

192

Amino Acids

21.03

Weight (kDa)

8.63

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 72 - 188 5e-39 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 95, 367
AccB1I GGYRCC 2 cut(s) 95, 367
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 2 cut(s) 65, 419
AfaI GTAC 6 cut(s) 97, 156, 248, 369, 450, 569
AfiI CCNNNNNNNGG 2 cut(s) 48, 524
AgsI TTSAA 3 cut(s) 141, 278, 574
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 4 cut(s) 54, 89, 161, 395
AluI AGCT 4 cut(s) 54, 89, 161, 395
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 16, 41
ApeKI GCWGC 3 cut(s) 5, 434, 514
Asp700I GAANNNNTTC 1 cut(s) 352
Asp718I GGTACC 2 cut(s) 95, 367
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 54, 394
AvaII GGWCC 1 cut(s) 425
BanI GGYRCC 2 cut(s) 95, 367
BanII GRGCYC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 17, 446, 526
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 2 cut(s) 16, 41
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 2 cut(s) 33, 258
BisI GCNGC 3 cut(s) 6, 435, 515
BlsI GCNGC 3 cut(s) 7, 436, 516
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 2 cut(s) 105, 237
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 3 cut(s) 97, 355, 369
BmrFI CCNGG 2 cut(s) 105, 237
BpuEI CTTGAG 1 cut(s) 375
BpuMI CCSGG 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 209, 235, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 524
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 209, 235, 250
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 48, 524
BseMI GCAATG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 17, 446, 526
BshNI GGYRCC 2 cut(s) 95, 367
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 105, 170
BslI CCNNNNNNNGG 2 cut(s) 48, 524
BsmAI GTCTC 2 cut(s) 16, 41
Bsp1286I GDGCHC 1 cut(s) 56
BspACI CCGC 2 cut(s) 65, 419
BspLI GGNNCC 3 cut(s) 97, 355, 369
BspT107I GGYRCC 2 cut(s) 95, 367
BsrDI GCAATG 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 209, 235, 250
BssT1I CCWWGG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 567
BstC8I GCNNGC 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 250
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 1 cut(s) 219
BstMAI GTCTC 2 cut(s) 16, 41
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 1 cut(s) 237
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 103, 235
BstV1I GCAGC 3 cut(s) 17, 446, 526
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 425
CseI GACGC 1 cut(s) 310
Csp6I GTAC 6 cut(s) 96, 155, 247, 368, 449, 568
CspCI CAANNNNNGTGG 2 cut(s) 360, 395
CviAII CATG 2 cut(s) 400, 530
CviJI RGCY 8 cut(s) 54, 89, 161, 388, 395, 489, 517, 534
CviKI_1 RGCY 8 cut(s) 54, 89, 161, 388, 395, 489, 517, 534
CviQI GTAC 6 cut(s) 96, 155, 247, 368, 449, 568
DraI TTTAAA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 2 cut(s) 403, 533
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 2 cut(s) 399, 529
Fnu4HI GCNGC 3 cut(s) 6, 435, 515
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 3 cut(s) 6, 435, 515
FspBI CTAG 2 cut(s) 33, 258
GluI GCNGC 3 cut(s) 6, 435, 515
HapII CCGG 2 cut(s) 105, 170
HgaI GACGC 1 cut(s) 310
Hin1II CATG 2 cut(s) 403, 533
HindIII AAGCTT 2 cut(s) 159, 393
HpaII CCGG 2 cut(s) 105, 170
HphI GGTGA 2 cut(s) 54, 394
Hpy188I TCNGA 1 cut(s) 123
HpyCH4III ACNGT 1 cut(s) 567
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 2 cut(s) 434, 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 2 cut(s) 403, 533
KpnI GGTACC 2 cut(s) 99, 371
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 6 cut(s) 118, 183, 222, 249, 290, 326
Lsp1109I GCAGC 3 cut(s) 17, 446, 526
MaeI CTAG 2 cut(s) 33, 258
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 3 cut(s) 60, 230, 550
MboII GAAGA 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 324, 372, 403
MmeI TCCRAC 3 cut(s) 202, 325, 403
MnlI CCTC 6 cut(s) 34, 84, 358, 399, 462, 518
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 2 cut(s) 192, 506
MslI CAYNNNNRTG 1 cut(s) 314
MspI CCGG 2 cut(s) 105, 170
MspR9I CCNGG 2 cut(s) 105, 237
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 105
NlaIII CATG 2 cut(s) 403, 533
NlaIV GGNNCC 3 cut(s) 97, 355, 369
NmuCI GTSAC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 352
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 3 cut(s) 7, 436, 516
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 235
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 97, 355, 369
PspPI GGNCC 1 cut(s) 425
RsaI GTAC 6 cut(s) 97, 156, 248, 369, 450, 569
RsaNI GTAC 6 cut(s) 96, 155, 247, 368, 449, 568
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 2 cut(s) 192, 506
SatI GCNGC 3 cut(s) 6, 435, 515
Sau96I GGNCC 1 cut(s) 425
ScaI AGTACT 1 cut(s) 569
ScrFI CCNGG 2 cut(s) 105, 237
SduI GDGCHC 1 cut(s) 56
SetI ASST 8 cut(s) 56, 91, 163, 355, 369, 397, 454, 460
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 390
SmoI CTYRAG 1 cut(s) 390
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 3 cut(s) 324, 372, 403
SsiI CCGC 2 cut(s) 65, 419
SspMI CTAG 2 cut(s) 33, 258
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 103, 235
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 567
TaiI ACGT 1 cut(s) 460
TaqI TCGA 1 cut(s) 76
TasI AATT 3 cut(s) 324, 372, 403
TatI WGTACW 2 cut(s) 154, 567
Tru1I TTAA 2 cut(s) 192, 506
Tru9I TTAA 2 cut(s) 192, 506
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 3 cut(s) 5, 434, 514
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 3 cut(s) 332, 416, 576
TspGWI ACGGA 1 cut(s) 267
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 2 cut(s) 33, 258
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.