RLG00000002007

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003153
Physical Location & Seq
Forward (+)
24270984 .. 24274402
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002007

Sequence Viewer

Length: 810 bp
ATGGCAGCAGTCTCTCTCTTTTCCTCTGCAACTAGTCTCCCCACCCTTTGGAGCCATCGTCGCCTCAGCTCGCTTTCACCGCCATTGCCTCGAACCGAAGAAGCTCTCGGTACCCTACCCGGTAGAACTCGTTTTCTGAACAAGTGTTCTGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGCCGGAGAATAGCGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCGCTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACGTCATATATTATTGGTTTATGATGAGATGAGCCTACCAAATGGTGTTCTAAGATTGCAGCCAAAAGGAGGACATGGCTATCACAATGGTGTGAAGAGTGTAGTGGGCCATCTGGATGGCTGCCGTGATTTTCCTCGATTATGTATAGGTATTGGAAATCCACCTGGTACTATGGACGTGAAAGCCTATCTTCTACAAAAATTCAGTACAGTAGAAAGACATCAGATTGATGCAGCACTGGGTCAAGGCGTTGAGGCTGTGAAGACACTGGTGCTGAATGGATTTGACCCGAATATCACTAGTTTCAATTTGAGGCAAAAATACAAGTATCACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

270

Amino Acids

29.44

Weight (kDa)

9.47

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 76 - 259 1.9e-56 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 110, 379
AccB1I GGYRCC 2 cut(s) 110, 379
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 2 cut(s) 80, 431
AcsI RAATTY 1 cut(s) 668
AdeI CACNNNGTG 1 cut(s) 805
AfaI GTAC 7 cut(s) 112, 171, 260, 381, 462, 637, 676
AfiI CCNNNNNNNGG 2 cut(s) 48, 536
AgsI TTSAA 3 cut(s) 156, 290, 775
AhlI ACTAGT 2 cut(s) 32, 767
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 2 cut(s) 247, 631
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 4 cut(s) 69, 104, 176, 407
AluI AGCT 4 cut(s) 69, 104, 176, 407
Alw26I GTCTC 2 cut(s) 16, 41
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 5 cut(s) 5, 446, 526, 588, 701
ApoI RAATTY 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 364
Asp718I GGTACC 2 cut(s) 110, 379
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 2 cut(s) 437, 574
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 69, 406
AvaII GGWCC 1 cut(s) 437
BanI GGYRCC 2 cut(s) 110, 379
BbsI GAAGAC 1 cut(s) 737
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 5 cut(s) 17, 458, 538, 575, 713
BccI CCATC 3 cut(s) 63, 578, 585
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 2 cut(s) 249, 633
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 16, 41
BcuI ACTAGT 2 cut(s) 32, 767
BfaI CTAG 3 cut(s) 33, 270, 768
BfoI RGCGCY 1 cut(s) 353
BisI GCNGC 5 cut(s) 6, 447, 527, 589, 702
BlsI GCNGC 5 cut(s) 7, 448, 528, 590, 703
Bme1390I CCNGG 3 cut(s) 120, 249, 633
Bme18I GGWCC 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 437, 574
BmiI GGNNCC 4 cut(s) 53, 112, 367, 381
BmrFI CCNGG 3 cut(s) 120, 249, 633
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 1 cut(s) 688
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 1 cut(s) 737
Bpu10I CCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 387
BpuMI CCSGG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 221, 247, 262
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 536
Bse1I ACTGG 2 cut(s) 711, 741
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 2 cut(s) 249, 633
BseDI CCNNGG 3 cut(s) 221, 247, 262
BseGI GGATG 2 cut(s) 231, 589
BseLI CCNNNNNNNGG 2 cut(s) 48, 536
BseMI GCAATG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 2 cut(s) 711, 741
BseXI GCAGC 5 cut(s) 17, 458, 538, 575, 713
BshFI GGCC 1 cut(s) 576
BshNI GGYRCC 2 cut(s) 110, 379
BsiSI CCGG 2 cut(s) 120, 185
BslI CCNNNNNNNGG 2 cut(s) 48, 536
BsmAI GTCTC 2 cut(s) 16, 41
BsnI GGCC 1 cut(s) 576
Bsp1407I TGTACA 1 cut(s) 169
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 2 cut(s) 80, 431
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 53, 112, 367, 381
BspT107I GGYRCC 2 cut(s) 110, 379
BsrDI GCAATG 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 169
BsrI ACTGG 2 cut(s) 711, 741
BssECI CCNNGG 3 cut(s) 221, 247, 262
BssT1I CCWWGG 2 cut(s) 221, 262
Bst2UI CCWGG 2 cut(s) 249, 633
Bst4CI ACNGT 1 cut(s) 679
Bst6I CTCTTC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 65, 518
BstDSI CCRYGG 1 cut(s) 262
BstEII GGTNACC 1 cut(s) 242
BstF5I GGATG 2 cut(s) 231, 589
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 1 cut(s) 352
BstMAI GTCTC 2 cut(s) 16, 41
BstMWI GCNNNNNNNGC 2 cut(s) 60, 79
BstNI CCWGG 2 cut(s) 249, 633
BstPI GGTNACC 1 cut(s) 242
BstSCI CCNGG 3 cut(s) 118, 247, 631
BstV1I GCAGC 5 cut(s) 17, 458, 538, 575, 713
BstV2I GAAGAC 1 cut(s) 737
BstXI CCANNNNNNTGG 1 cut(s) 584
BsuRI GGCC 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 262
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 2 cut(s) 231, 589
BtsIMutI CAGTG 2 cut(s) 704, 734
Cac8I GCNNGC 1 cut(s) 71
CfoI GCGC 1 cut(s) 352
Cfr13I GGNCC 2 cut(s) 437, 574
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 7 cut(s) 111, 170, 259, 380, 461, 636, 675
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 3 cut(s) 263, 412, 542
CviQI GTAC 7 cut(s) 111, 170, 259, 380, 461, 636, 675
DdeI CTNAG 2 cut(s) 65, 518
DraI TTTAAA 1 cut(s) 205
DraIII CACNNNGTG 1 cut(s) 805
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 221, 262
Eco47I GGWCC 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 247, 631
EcoT14I CCWWGG 2 cut(s) 221, 262
ErhI CCWWGG 2 cut(s) 221, 262
FaeI CATG 3 cut(s) 266, 415, 545
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FatI CATG 3 cut(s) 262, 411, 541
Fnu4HI GCNGC 5 cut(s) 6, 447, 527, 589, 702
FokI GGATG 2 cut(s) 238, 596
Fsp4HI GCNGC 5 cut(s) 6, 447, 527, 589, 702
FspBI CTAG 3 cut(s) 33, 270, 768
GlaI GCGC 1 cut(s) 351
GluI GCNGC 5 cut(s) 6, 447, 527, 589, 702
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 1 cut(s) 576
HapII CCGG 2 cut(s) 120, 185
HhaI GCGC 1 cut(s) 352
Hin1II CATG 3 cut(s) 266, 415, 545
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 2 cut(s) 174, 405
HpaII CCGG 2 cut(s) 120, 185
HphI GGTGA 2 cut(s) 69, 406
Hpy188I TCNGA 2 cut(s) 138, 693
Hpy188III TCNNGA 1 cut(s) 581
Hpy99I CGWCG 1 cut(s) 63
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4IV ACGT 2 cut(s) 469, 645
HpyCH4V TGCA 4 cut(s) 29, 446, 526, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 79
HpyF3I CTNAG 2 cut(s) 65, 518
HpySE526I ACGT 2 cut(s) 469, 645
Hsp92II CATG 3 cut(s) 266, 415, 545
HspAI GCGC 1 cut(s) 350
KpnI GGTACC 2 cut(s) 114, 383
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 5 cut(s) 17, 458, 538, 575, 713
LweI GCATC 1 cut(s) 688
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 3 cut(s) 33, 270, 768
MaeII ACGT 2 cut(s) 469, 645
MaeIII GTNAC 1 cut(s) 242
MboII GAAGA 4 cut(s) 110, 574, 650, 742
MluCI AATT 5 cut(s) 336, 384, 415, 668, 775
MmeI TCCRAC 3 cut(s) 214, 337, 415
MroXI GAANNNNTTC 1 cut(s) 364
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 3 cut(s) 326, 555, 582
MspI CCGG 2 cut(s) 120, 185
MspR9I CCNGG 3 cut(s) 120, 249, 633
MvaI CCWGG 2 cut(s) 249, 633
MwoI GCNNNNNNNGC 2 cut(s) 60, 79
NciI CCSGG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 262
NlaIII CATG 3 cut(s) 266, 415, 545
NlaIV GGNNCC 4 cut(s) 53, 112, 367, 381
OliI CACNNNNGTG 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 364
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 5 cut(s) 7, 448, 528, 590, 703
Psp6I CCWGG 2 cut(s) 247, 631
PspEI GGTNACC 1 cut(s) 242
PspGI CCWGG 2 cut(s) 247, 631
PspN4I GGNNCC 4 cut(s) 53, 112, 367, 381
PspPI GGNCC 2 cut(s) 437, 574
RsaI GTAC 7 cut(s) 112, 171, 260, 381, 462, 637, 676
RsaNI GTAC 7 cut(s) 111, 170, 259, 380, 461, 636, 675
RseI CAYNNNNRTG 3 cut(s) 326, 555, 582
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 5 cut(s) 6, 447, 527, 589, 702
Sau96I GGNCC 2 cut(s) 437, 574
ScrFI CCNGG 3 cut(s) 120, 249, 633
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 1 cut(s) 688
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 3 cut(s) 326, 555, 582
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
SpeI ACTAGT 2 cut(s) 32, 767
Sse9I AATT 5 cut(s) 336, 384, 415, 668, 775
SsiI CCGC 2 cut(s) 80, 431
SspMI CTAG 3 cut(s) 33, 270, 768
StyD4I CCNGG 3 cut(s) 118, 247, 631
StyI CCWWGG 2 cut(s) 221, 262
TaaI ACNGT 1 cut(s) 679
TaiI ACGT 2 cut(s) 472, 648
TaqI TCGA 2 cut(s) 91, 604
TasI AATT 5 cut(s) 336, 384, 415, 668, 775
TatI WGTACW 2 cut(s) 169, 674
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 711, 741
TseI GCWGC 5 cut(s) 5, 446, 526, 588, 701
TspDTI ATGAA 2 cut(s) 344, 428
TspRI CASTG 2 cut(s) 711, 741
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 364
XspI CTAG 3 cut(s) 33, 270, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.