MD17G1245600.v1.1

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003153
Physical Location & Seq
Reverse (-)
29391697 .. 29396102
4406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1245600.v1.1.491

Sequence Viewer

Length: 930 bp
ATGAAAATAAATCTTAGATCTTTCTTATTAACTCTATCCTCTGAAACACCTCCGTCTGCGACTGCAAACCATAAACAACTTCCAAGCTCTCGTCGAGAAGCACCTCCTCTCTCTCTCTCCAACTTCCTCACATTCATATATCCCCACTTCAAATACAAGCTCCAAAATTTGCAGCCAGGTTCGTCAATGTGGTATGCTGTCTCTGCCCCAAACTATTGCATATCATATCCGAGAAGACCTTGCAGCCCTCCTTCTCTAAGGAAGTATTCGGTTTCCACAAGTTTACGTGTGAAGGCTTGCTTACCGGATAATACCAATGGCGCTAAAGTGGAGTACACTCCTTGGCTGATTGTTGGATTGGGTAACCCTGGCAACAAGTACCATGGGACTAGGCACAATATTGGTTTCGAGATGATTGATGGTATTGCTAAAGCAGAAGGCATTGCGATGAATACAATACAGTCGAAGGCCTTGATTGGAATAGGCTCCATTGGAGAAGTACCAATTTTGTTGGTGAAGCCTCAAGCCTACATGAATTATAGCGGAGAATCGGTTGGACCACTTGCGGCATATTATCAAGTACCCCTACGCCATATTTTACTGGTTTACGATGAGATGAGCTTACCAAATGGTGTTCTGAGGATTCAGCCAAAAGGTGGTCATGGCTATCACAATGGTGTAAAAAGTGTAATGGGCCATCTGGACGGTTGCCGTGAATTTCCTCGGCTGTGTATAGGTATTGGAAATCCACCTGGAACTATGGACATGAAAGCCTATCTTCTACAGAGATTCAGTACAGTCGAGAGACATCAGATTGATGCAGCACTGGATCAAGGGGTTGAAGCTGTAAGGACCCTGGTGCTGAATGGATTTGACCAGAGCATCACTAGTTTTAATTTGAGGCAGAAATACAAATATCACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

310

Amino Acids

34.28

Weight (kDa)

9.56

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 116 - 299 3.2e-58 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 656
AciI CCGC 2 cut(s) 543, 566
AclWI GGATC 1 cut(s) 837
AcsI RAATTY 2 cut(s) 166, 716
AdeI CACNNNGTG 1 cut(s) 925
AfaI GTAC 5 cut(s) 335, 380, 501, 582, 796
AfiI CCNNNNNNNGG 1 cut(s) 656
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 2 cut(s) 151, 842
AhlI ACTAGT 1 cut(s) 887
AjnI CCWGG 4 cut(s) 175, 367, 751, 855
AleI CACNNNNGTG 1 cut(s) 675
AluBI AGCT 4 cut(s) 87, 160, 621, 845
AluI AGCT 4 cut(s) 87, 160, 621, 845
Alw26I GTCTC 2 cut(s) 205, 799
AlwI GGATC 1 cut(s) 837
AoxI GGCC 2 cut(s) 468, 694
ApeKI GCWGC 3 cut(s) 172, 243, 821
ApoI RAATTY 2 cut(s) 166, 716
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 3 cut(s) 557, 694, 852
AsuHPI GGTGA 1 cut(s) 526
AvaII GGWCC 2 cut(s) 557, 852
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 3 cut(s) 184, 255, 833
BccI CCATC 2 cut(s) 413, 705
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 4 cut(s) 177, 369, 753, 857
BcoDI GTCTC 2 cut(s) 205, 799
BcuI ACTAGT 1 cut(s) 887
BfaI CTAG 2 cut(s) 390, 888
BfmI CTRYAG 1 cut(s) 782
BfoI RGCGCY 1 cut(s) 324
BglII AGATCT 1 cut(s) 17
BisI GCNGC 4 cut(s) 173, 244, 567, 822
BlsI GCNGC 4 cut(s) 174, 245, 568, 823
Bme1390I CCNGG 4 cut(s) 177, 369, 753, 857
Bme18I GGWCC 2 cut(s) 557, 852
BmgT120I GGNCC 3 cut(s) 557, 694, 852
BmiI GGNNCC 2 cut(s) 487, 854
BmrFI CCNGG 4 cut(s) 177, 369, 753, 857
BmsI GCATC 2 cut(s) 808, 891
BpiI GAAGAC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 287
BsaJI CCNNGG 5 cut(s) 341, 367, 382, 722, 855
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 606, 831
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 4 cut(s) 177, 369, 753, 857
BseDI CCNNGG 5 cut(s) 341, 367, 382, 722, 855
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 2 cut(s) 606, 831
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 3 cut(s) 184, 255, 833
BshFI GGCC 2 cut(s) 470, 696
BsiSI CCGG 1 cut(s) 305
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 2 cut(s) 205, 799
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 2 cut(s) 470, 696
Bsp143I GATC 2 cut(s) 17, 829
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 2 cut(s) 543, 566
BspANI GGCC 2 cut(s) 470, 696
BspCNI CTCAG 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 487, 854
BspPI GGATC 1 cut(s) 837
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 2 cut(s) 606, 831
BssECI CCNNGG 5 cut(s) 341, 367, 382, 722, 855
BssMI GATC 2 cut(s) 17, 829
BssT1I CCWWGG 2 cut(s) 341, 382
Bst2UI CCWGG 4 cut(s) 177, 369, 753, 857
Bst4CI ACNGT 3 cut(s) 462, 707, 799
BstBAI YACGTR 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 3 cut(s) 14, 257, 638
BstDSI CCRYGG 1 cut(s) 382
BstEII GGTNACC 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 2 cut(s) 20, 832
BstMAI GTCTC 2 cut(s) 205, 799
BstMBI GATC 2 cut(s) 17, 829
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstNI CCWGG 4 cut(s) 177, 369, 753, 857
BstPI GGTNACC 1 cut(s) 362
BstSCI CCNGG 4 cut(s) 175, 367, 751, 855
BstSFI CTRYAG 1 cut(s) 782
BstV1I GCAGC 3 cut(s) 184, 255, 833
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 2 cut(s) 470, 696
BtgI CCRYGG 1 cut(s) 382
BtgZI GCGATG 1 cut(s) 461
BtsIMutI CAGTG 1 cut(s) 824
Cac8I GCNNGC 1 cut(s) 298
CfoI GCGC 1 cut(s) 323
Cfr13I GGNCC 3 cut(s) 557, 694, 852
Csp6I GTAC 5 cut(s) 334, 379, 500, 581, 795
CviAII CATG 4 cut(s) 383, 532, 662, 766
CviQI GTAC 5 cut(s) 334, 379, 500, 581, 795
DdeI CTNAG 3 cut(s) 14, 257, 638
DpnI GATC 2 cut(s) 19, 831
DpnII GATC 2 cut(s) 17, 829
DraIII CACNNNGTG 1 cut(s) 925
Eco130I CCWWGG 2 cut(s) 341, 382
Eco147I AGGCCT 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 557, 852
Eco91I GGTNACC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 852
EcoO65I GGTNACC 1 cut(s) 362
EcoRII CCWGG 4 cut(s) 175, 367, 751, 855
EcoT14I CCWWGG 2 cut(s) 341, 382
ErhI CCWWGG 2 cut(s) 341, 382
FaeI CATG 4 cut(s) 386, 535, 665, 769
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 762, 794
FaqI GGGAC 1 cut(s) 400
FatI CATG 4 cut(s) 382, 531, 661, 765
Fnu4HI GCNGC 4 cut(s) 173, 244, 567, 822
Fsp4HI GCNGC 4 cut(s) 173, 244, 567, 822
FspBI CTAG 2 cut(s) 390, 888
GlaI GCGC 1 cut(s) 322
GluI GCNGC 4 cut(s) 173, 244, 567, 822
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 2 cut(s) 470, 696
HapII CCGG 1 cut(s) 305
HhaI GCGC 1 cut(s) 323
Hin1II CATG 4 cut(s) 386, 535, 665, 769
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 3 cut(s) 548, 643, 789
HpaII CCGG 1 cut(s) 305
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 3 cut(s) 284, 336, 607
Hpy188I TCNGA 4 cut(s) 43, 231, 639, 813
Hpy188III TCNNGA 4 cut(s) 95, 409, 701, 802
Hpy8I GTNNAC 3 cut(s) 284, 336, 607
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 4 cut(s) 261, 286, 431, 460
HpyCH4III ACNGT 3 cut(s) 462, 707, 799
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 5 cut(s) 65, 172, 219, 243, 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpyF3I CTNAG 3 cut(s) 14, 257, 638
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 4 cut(s) 386, 535, 665, 769
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 2 cut(s) 17, 829
LmnI GCTCC 2 cut(s) 165, 491
Lsp1109I GCAGC 3 cut(s) 184, 255, 833
LweI GCATC 2 cut(s) 808, 891
MaeI CTAG 2 cut(s) 390, 888
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 362
MalI GATC 2 cut(s) 19, 831
MboI GATC 2 cut(s) 17, 829
MboII GAAGA 2 cut(s) 246, 770
MflI RGATCY 1 cut(s) 17
MluCI AATT 5 cut(s) 166, 504, 535, 716, 895
MmeI TCCRAC 3 cut(s) 144, 334, 535
MseI TTAA 2 cut(s) 29, 894
MslI CAYNNNNRTG 2 cut(s) 446, 675
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 4 cut(s) 177, 369, 753, 857
MvaI CCWGG 4 cut(s) 177, 369, 753, 857
MwoI GCNNNNNNNGC 1 cut(s) 203
NcoI CCATGG 1 cut(s) 382
NdeII GATC 2 cut(s) 17, 829
NlaIII CATG 4 cut(s) 386, 535, 665, 769
NlaIV GGNNCC 2 cut(s) 487, 854
NmeAIII GCCGAG 1 cut(s) 703
OliI CACNNNNGTG 1 cut(s) 675
PceI AGGCCT 1 cut(s) 470
PfeI GAWTC 3 cut(s) 548, 643, 789
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 4 cut(s) 174, 245, 568, 823
Ppu21I YACGTR 1 cut(s) 287
PpuMI RGGWCCY 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 852
Psp6I CCWGG 4 cut(s) 175, 367, 751, 855
PspEI GGTNACC 1 cut(s) 362
PspGI CCWGG 4 cut(s) 175, 367, 751, 855
PspN4I GGNNCC 2 cut(s) 487, 854
PspPI GGNCC 3 cut(s) 557, 694, 852
PspPPI RGGWCCY 1 cut(s) 852
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 5 cut(s) 335, 380, 501, 582, 796
RsaNI GTAC 5 cut(s) 334, 379, 500, 581, 795
RseI CAYNNNNRTG 2 cut(s) 446, 675
SaqAI TTAA 2 cut(s) 29, 894
SatI GCNGC 4 cut(s) 173, 244, 567, 822
Sau3AI GATC 2 cut(s) 17, 829
Sau96I GGNCC 3 cut(s) 557, 694, 852
ScrFI CCNGG 4 cut(s) 177, 369, 753, 857
SfaNI GCATC 2 cut(s) 808, 891
SfcI CTRYAG 1 cut(s) 782
SinI GGWCC 2 cut(s) 557, 852
SmiMI CAYNNNNRTG 2 cut(s) 446, 675
SmlI CTYRAG 1 cut(s) 522
SmoI CTYRAG 1 cut(s) 522
SpeI ACTAGT 1 cut(s) 887
Sse9I AATT 5 cut(s) 166, 504, 535, 716, 895
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 2 cut(s) 543, 566
SspI AATATT 1 cut(s) 400
SspMI CTAG 2 cut(s) 390, 888
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 4 cut(s) 175, 367, 751, 855
StyI CCWWGG 2 cut(s) 341, 382
TaaI ACNGT 3 cut(s) 462, 707, 799
TaiI ACGT 1 cut(s) 289
TaqI TCGA 4 cut(s) 94, 408, 464, 801
TasI AATT 5 cut(s) 166, 504, 535, 716, 895
TatI WGTACW 2 cut(s) 333, 794
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 3 cut(s) 548, 643, 789
Tru1I TTAA 2 cut(s) 29, 894
Tru9I TTAA 2 cut(s) 29, 894
TscAI CASTG 1 cut(s) 831
TseI GCWGC 3 cut(s) 172, 243, 821
TspDTI ATGAA 5 cut(s) 17, 124, 464, 548, 782
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 1 cut(s) 831
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 557, 852
XapI RAATTY 2 cut(s) 166, 716
XspI CTAG 2 cut(s) 390, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.