Rorug06G0151900

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003153
Physical Location & Seq
Reverse (-)
21731958 .. 21733089
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0151900.1

Sequence Viewer

Length: 291 bp
ATGAAATTGACCAAGTCAACTCTCGCATACGACAGGGAAGATGAAACTGTTGAGATGTATGAGGATAGCGATAATGCGAGCAAGAACGAGTTTGAGGCTCTGCTCTCGGAAATTGTGAGATTGGAACTTATCACTTGCCTTCAGACGATTGATAGCAACAACTATCCTGAGACTTTGAATCGTATGTTTATCATCAATGCTGGTTCTGGTTTTAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACATCTGAAAAGATTAATGTCAGTATACCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

96

Amino Acids

11.05

Weight (kDa)

4.54

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 43 - 92 2.8e-12 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 280
AclWI GGATC 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 125
AgsI TTSAA 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 242
AseI ATTAAT 1 cut(s) 270
Asp700I GAANNNNTTC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 164
BseMII CTCAG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 160
BspPI GGATC 1 cut(s) 242
BssMI GATC 1 cut(s) 247
BssNAI GTATAC 1 cut(s) 281
Bst1107I GTATAC 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 49, 232
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 168
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 247
BstZ17I GTATAC 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 79
CviJI RGCY 1 cut(s) 98
CviKI_1 RGCY 1 cut(s) 98
DdeI CTNAG 1 cut(s) 168
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 125
FaiI YATR 4 cut(s) 28, 60, 185, 281
FblI GTMKAC 1 cut(s) 280
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 18, 281
Hpy188I TCNGA 3 cut(s) 109, 144, 262
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 2 cut(s) 18, 281
HpyAV CCTTC 2 cut(s) 149, 250
HpyCH4III ACNGT 2 cut(s) 49, 232
HpyF3I CTNAG 1 cut(s) 168
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 4 cut(s) 19, 180, 186, 192
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 2 cut(s) 5, 111
MnlI CCTC 2 cut(s) 55, 88
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 1 cut(s) 270
NdeII GATC 1 cut(s) 247
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 13
PshBI ATTAAT 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 270
Sau3AI GATC 1 cut(s) 247
SduI GDGCHC 1 cut(s) 230
SetI ASST 1 cut(s) 286
Sse9I AATT 2 cut(s) 5, 111
TaaI ACNGT 2 cut(s) 49, 232
TasI AATT 2 cut(s) 5, 111
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 17, 57
TspRI CASTG 1 cut(s) 235
Tth111I GACNNNGTC 1 cut(s) 13
VspI ATTAAT 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.